EFFECT OF SEQUENCE OF PREGNANCY ON LITTER SIZE AND GROWTH IN PEROMYSCUS POLIONOTUS.
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At autopsy the absolute and relative testicular weights of 6-week-old white-footed mice were significantly lower (P < 0.001) in animals exposed to a short photoperiod (8L : 16D) or exposed to a long photoperiod (16L : 8D) and treated with a 1 mg melatonin s.c. implant than in those kept only in a long photoperiod. The long photoperiod animals had tests of normal appearance and complete spermatogenesis, but there had been little development in the testes of the other animals and seminiferous tubule diameters were reduced.
Male white-footed mice were maintained from birth in chambers equipped with latitudinal timers programmed to simulate the natural progression of daylengths at 42 degrees N latitude. Mice were born into photoperiods starting on 1 April, 15 July, 15 September or 27 October. Testes and seminal vesicles of April- and July-born mice were mature at 60 days of age; puberty was delayed by several months in animals born into the September and October photoperiods but these mice became fecund at about 3.5 months of age, independent of current photoperiod. Therefore, increasing daylengths of late winter do not appear to synchronize the reproductive development of overwintering juveniles born at different times during the previous breeding season.
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Small mammals were trapped in northeastern Alberta, Canada during 1976. Blood samples from these animals were tested for virus by inoculation of suckling mice. Blood clots from two deer mice yielded isolates of the same virus. The virus was related antigenically to a number of flaviviruses which have been isolated from mammals in Central America and North America and was related most closely to Modoc virus. Physical, chemical, and biological properties of the virus were similar also to those of Modoc virus. It did not produce illness or death in deer mice inoculated in the laboratory. Neutralization tests indicated that 1/38 (3%) red squirrels (Tamiasciurus hudsonicus), 3/35 (9%) least chipmunks (Eutamius minimus), 13/109 (12%) deer mice, and 3/50 (6%) humans were infected naturally. This is the first reported evidence of infection of red squirrels and chipmunks with a Modoc-like virus. These data extend the range of Modoc-like viruses northward by 1,500 km and comprise the first isolate from mammals in the boreal forest of Canada.
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