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At least 487 records · Page 27Linked to original sources
PubMed2001

A molecular phylogenetic survey of sea-ice microbial communities (SIMCO).

16S rDNA clone library analysis was used to identify bacterial biodiversity in a variety of sea-ice microbial communities (SIMCO). DNA was extracted from seven Antarctic sea-ice samples and one Arctic sea-ice sample and 16S rDNA PCR-amplified using universal and Archaea-specific primers. Recombinant 16S rDNA clones were obtained and dereplicated using restriction fragment length polymorphism analysis (RFLP). After RFLP analysis, 100 distinct phylotypes (a unique clone or group of clones with sequence similarity of >0.98) were defined. From the clone libraries 16S rDNA sequences of bacterial and eukaryotic origin were detected, however Archaea were not detected either with universal or Archaea-specific 16S rDNA primer sets. Bacterial phylotypes grouped within the alpha and gamma proteobacteria, the Cytophaga-Flavobacterium-Bacteroides division, the Gram-Positive bacteria and the orders Chlamydiales and Verrucomicrobiales. The majority of bacterial phylotypes were affiliated with heterotrophic taxa and many grouped closely with cultivated genera and species. Eukaryotic clones were affiliated with a variety of autotrophic and heterotrophic nanoplankton and included a large number of chloroplast 16S rDNA genes. The findings of this investigation corroborated culture data indicating bacterial biodiversity increased in SIMCO displaying high levels of primary production, however the bacterial communities within SIMCO were highly heterogeneous at the genus/species-level between different samples. A comparison of Antarctic and Arctic SIMCO revealed certain sea-ice dwelling bacterial genera are common at both poles.

Journal Article↗
PubMed1972

Observations on the AIM-TWX service at the University of Virginia Medical Library.

This paper is an analysis of 100 short questionaires which were completed by patrons of the AIM-TWX computerized, on-line bibliographical search service at the Medical Library of the University of Virginia during the period from August 1971 to January 1972. The survey indicates strengths and weaknesses of the new system.

Computers↗
PubMed1991

Biotechnology awareness study, Part 2: Meeting the information needs of biotechnologists.

The second part of the biotechnology awareness study focused on health sciences libraries and how well they are meeting the needs of biotechnologists working in the study's nine medical centers. A survey was conducted over a three-month period to assess the demand for biotechnology-related reference services at nine libraries and the sources the librarians used to answer the questions. Data on monographic and current serial holdings were also collected. At the end of the survey period, librarians were asked for their perceptions about biotechnology research at their institutions and in their geographic areas. Their responses were compared to the responses the scientists at the nine schools gave to the same or similar questions. Results showed few biotechnology-related reference questions were asked of the librarians. The recorded questions dealt with a range of biotechnology subjects. MEDLINE was used to answer 77% of the questions received during the survey period. More detailed notes in MeSH and a guide to online searching for biotechnology topics were suggested by the librarians as ways to improve reference service to this group of researchers. Journal collections were generally strong, with libraries owning from 50% to 87% of the titles on a core list of biotechnology journals compiled for this study. All libraries subscribed to the five titles most often cited by the scientists surveyed. Generally, librarians were unaware of the biotechnology-related research being done on their campuses or in their geographic areas.

Awareness↗
PubMed2006

Brazilian plants with possible action on the central nervous system: a study of historical sources from the 16th to 19th century.

Brazil is a country rich in biodiversity, endemism, and cultural diversity, inhabited by different types of population. European expeditions and the migratory processes that began in the 16th century greatly contributed both to cultural diversity and to Brazilian popular therapeutics, and produced the first records on medicinal plants in Brazil. This study comprises a bibliographical survey of historic books found in Sao Paulo libraries (16th through 19th centuries) on medicinal plants exerting effects on the central nervous system (CNS). Thirty-four plants native to Brazil were selected from the reading of the books. Of these 34 plants, 13 were also recorded in ethnopharmacological studies among modern Brazilian communities and 16 have been studied phytochemically. Only eight have been the object of pharmacological studies, six of these, recently, with a request for a patent. Results showed that most of the species recorded in this study have been reported as medicinal for centuries, but have never been the object of pharmacological investigation down to the present time. Such results provide ideas for a selection of these species as potentially bioactive to be included in future pharmacological studies.

Books↗
PubMed2003

The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase.

The breast cancer-associated T2A10 clone was originally isolated from a cDNA library enriched for tumour messenger ribonucleic acids. Our survey of 125 microarrayed primary tumour tissues using affinity purified polyclonal antibodies has revealed that corresponding protein is overexpressed in invasive breast cancer and is weakly expressed in kidney and prostate tumours. Now known as RNF11, the gene encodes a RING-H2 domain and a PY motif, both of which mediate protein-protein interactions. In particular, the PPPPY sequence of RNF11 PY motif is identical to that of Smad7, which has been shown to bind to WW domains of Smurf2, an E3 ubiquitin ligase that mediates the ubiquitination and degradation of the TGFbeta receptor complex. Using various mutants of RNF11 in GST pulldown and immunoprecipitation assays, we found that RNF11 interacts with Smurf2 through the PY motif, leading to ubiquitination of both proteins. Smurf2 plays an active role in the repression of TGFbeta signalling, and our data indicate that overexpression of RNF11, through its interaction with Smurf2, can restore TGFbeta responsiveness in transfected cells.

Breast Neoplasms↗
PubMed1975

Home bases for private work and study. Are multidisciplinary laboratories the best answer?

Since 1970 we have measured the use of our multidisciplinary laboratories as home study bases for out-of-hours private work, and find that our students make little use of them in this way, preferring to work in study-bedrooms or libraries. We consider that there is no need to incorporate private study areas in the design of multidisciplinary laboratories, with the result that uncluttered areas with free sight lines can be used for a variety of purposes; we suggest that multidisciplinary laboratories will evolve in the future along this multi-purpose, multifunction line.

Curriculum↗
PubMed1992

Secondary data on health-related subjects: major sources, uses, and limitations.

Although secondary data are generally neglected by nurses, they are useful for nurse researchers, practitioners, educators, administrators, and policy makers. Major sources of data on health-related subjects are medical records, administrative records, the census, vital statistics, surveys of the National Center for Health Statistics, social science archives, and nursing archives, each with its specific uses and limitations.

Archives↗
PubMed2006

[Palliative care in cancer: elements for debating the guidelines].

The aim of this study was to present elements for debating guidelines on palliative care programs for cancer. A literature survey searched various databases (MEDLINE, LILACS, and Cochrane Library), homepages of palliative care organizations, publications by renowned authors in this area, reference textbooks on the subject, relevant articles cited by these books, and the thesis/dissertation database of CAPES (the Brazilian Coordinating Body for Training University Level Personnel). The data were grouped into four thematic categories: symptomatic palliation, organization of services, psychosocial characteristics, and spiritual characteristics. The article then proceeds to discuss cancer as a public health problem and its impact on individuals, the concept of palliative care and its context in health care, program models and their guidelines, death and dying, and care and caregivers. The study concludes by discussing the challenges for the Brazilian National Health System in structuring end-of-life programs as a consequence of the population's aging and increasing cancer incidence.

Attitude of Health Personnel↗
PubMed2006

Vignettes: Diverse library staff offering diverse bioinformatics services.

OBJECTIVES: The paper gives examples of the bioinformatics services provided in a variety of different libraries by librarians with a broad range of educational background and training. METHODS: Two investigators sent an email inquiry to attendees of the "National Center for Biotechnology Information's (NCBI) Introduction to Molecular Biology Information Resources" or "NCBI Advanced Workshop for Bioinformatics Information Specialists (NAWBIS)" courses. The thirty-five-item questionnaire addressed areas such as educational background, library setting, types and numbers of users served, and bioinformatics training and support services provided. Answers were compiled into program vignettes. DISCUSSION: The bioinformatics support services addressed in the paper are based in libraries with academic and clinical settings. Services have been established through different means: in collaboration with biology faculty as part of formal courses, through teaching workshops in the library, through one-on-one consultations, and by other methods. Librarians with backgrounds from art history to doctoral degrees in genetics have worked to establish these programs. CONCLUSION: Successful bioinformatics support programs can be established in libraries in a variety of different settings and by staff with a variety of different backgrounds and approaches.

Computational Biology↗
PubMed1991

[Literature searching on CD-ROM disks].

CD-ROM, Compact Disk Read Only Memory, is a powerful resource which combines the best features of both online and print access to the literature. In 1988 the Medical Library in Trondheim installed the Medline database from Dialog on CD-ROM for library users, and used the opportunity to launch a study to evaluate the new medium in the library (use, users, costs, etc.). The results are based on 651 questionnaires answered by 185 different users. Totally they searched for more than 1,200 different subjects during first six months that the library subscribed to the CD-ROM-disks. All users said that the system was easy to learn and to use (even the 50% who were not familiar with a PC). The study shows that retrieving information from CD-ROM databases is an easy and popular way to keep updated in a particular field.

CD-ROM↗
PubMed1988

Library use and information-seeking behavior of veterinary medical students.

Veterinary medical students at Iowa State University were assessed for general use of the veterinary library and for their information-seeking behavior. The library was most frequently used for studying and for making photocopies of materials. The typical respondent relied on course textbooks and handouts for current information on unfamiliar topics, instead of using indexes or abstracts for guidance to recent literature. Light use of library information resources raises the concern that students are developing an inadequate base of retrieval skills for finding information on new procedures, diseases and drugs. No differences were found between students with and without formal bibliographic instruction in their approaches to seeking information or in library use.

Attitude of Health Personnel↗
PubMed1997

Primary clientele as a predictor of interlibrary borrowing: a study of academic health sciences libraries.

Statistics obtained from the Association of Academic Health Sciences Library Directors (AAHSLD) member libraries were studied during 1991/92 to determine whether the size of the primary client pool is a better predictor of a library's interlibrary loan (ILL) borrowing than is collection size. In libraries with collections of fewer than 70,000 serial and monograph titles, size of the client base explained 25% of the variation in ILL borrowing. No measure of collection size helped explain a greater percentage of the variability. This relationship was not found in libraries with collections of more than 70,000 titles. Further research into the predictors of ILL borrowing volume in the relatively controlled environment of the OhioLINK system is proposed.

Faculty, Medical↗
PubMed1997

Health information networking via the Internet with the former Soviet Union.

Because of the severe financial hardships associated with the transition to a market economy in the Newly Independent States (NIS) of the former Soviet Union, the Internet has become a major link to health care resources for many health care workers. In 1992, the University of Illinois at Chicago Library of the Health Sciences (UIC LHS) initiated a special support project with goals of enhancing access to international biomedical information and facilitating international professional networking for interested NIS organizations and individuals. Project objectives included an information needs assessment, development of culturally sensitive Internet training applications, information and referral services, and follow-up e-mail consultations for NIS participants. This paper reviews the historical context of the health care partnerships between the United States and the NIS, and of the UIC LHS International Health Information Networking Project. In the context of UIC, international networking cross-cultural observations, teaching techniques, a Russian training experience, and the development of a Web-based course are reported.

Computer Communication Networks↗