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At least 487 records · Page 27Linked to original sources
PubMed1995

The cDNA sequence of the lactate dehydrogenase-A of the spiny dogfish (Squalus acanthias): corrections to the amino acid sequence and an analysis of the phylogeny of vertebrate lactate dehydrogenases.

The cDNA sequence of the lactate dehydrogenase-A (LDH-A) of the spiny dogfish was determined. The deduced amino acid sequence differed from a previously determined protein sequence by 5%. Separate maximum parsimony analyses of the two sequences along with LDHs of other vertebrates resulted in shorter trees with the sequence presented here, as well as fewer equally parsimonious trees. The new sequence also indicates a greater conservation of length among vertebrate LDHs than was previously suspected. Analyses of the phylogeny of vertebrate LDHs resulted in a monophyletic grouping of LDH-As, from within which mammalian LDH-C is derived. The phylogeny of LDH-As did not exactly match the phylogeny of the organisms, raising the possibility of multiple origins and losses of a muscle-predominant gene. LDH-Bs appear to have shared a single origin.

Amino Acid Sequence↗
PubMed1999

Classification and identification of strains of Lactobacillus brevis based on electrophoretic characterization of D-lactate dehydrogenase: relationship between D-lactate dehydrogenase and beer-spoilage ability.

The cell-free extracts of 60 strains which were identified phenotypically as being those of Lactobacillus brevis, including 48 isolates from the environment and 12 reference strains, were applied to polyacrylamide gel electrophoresis for extracting their NAD-dependent D- and L-lactate dehydrogenases (LDH). These strains were divided into 5 groups, i.e., Groups A, B, C, D, and E, on the basis of the electrophoretic mobilities of their D-LDH. The strains showed variations in their carbohydrate fermentation patterns. No relationship between the profile of D-LDH and the carbohydrate fermentation pattern was recognized. However, there appeared to be a relationship between the D-LDH profile and the beer-spoilage ability, because 40 out of 44 beer-spoilage strains identified as L. brevis were classified to Group B. We purified D-LDHs from the so-called complete beer-spoilage strain SBC 8002 of LDH Group B and from the non beer-spoilage strains JCM 1059T of LDH Group A and AHU 1508 of LDH Group C. Although the purified D-LDHs had the same molecular weight (84 kDa), each possessed a different optimum pH, optimum temperature, and isoelectric point. The aforementioned parameter values for the enzyme from the so-called complete beer-spoilage strain SBC 8002 of LDH Group B were 10.0, 50 degrees C, and 4.1, respectively; this strain was discriminated from the D-LDHs of the other two non beer-spoilage strains especially by its optimum temperature (50 degrees C).

Journal Article↗
PubMed2002

Is lactation mastitis and shooting breast pain experienced by women during lactation caused by Candida albicans?

The evidence relating Candida albicans with deep breast pain suffered by some women during breast feeding is reviewed. There is little direct evidence to support the aetiological role of Candida albicans in this condition, and therefore the prescription of fluconazole (an antifungal drug) to lactating women with deep breast pain as suggested by a number of authors of studies on this subject cannot be justified unless as part of a clinical trial.

Journal Article↗
PubMed2005

Saturation of the lactate clearance mechanisms different from the "lactate shuttle" determines the anaerobic threshold: prediction from the bioenergetic model.

It is demonstrated, that the bioenergetic model combined with the mathematical constraints determined by the experimental knowledge of the aerobic metabolism and the Lohmann reaction dictates the exact lactate (La)-time relationship during exercise. The theory predicts that La is necessarily produced (above the resting baseline), even during extremely low work loads, where the metabolism was usually considered in the past to be "pure" aerobic. The La rate of production increases linearly as a function of the work load. The anaerobic threshold is strictly determined by the saturation of the La clearance mechanisms of the body different from the "La shuttle" and not by the involvement of a sudden increased La production at the cellular level. These results imply that the half time of the PCr breakdown kinetics at the onset of a constant load exercise can be expressed as a function of the onset speed of the aerobic and of the anaerobic metabolism, even in the case of a very low mechanical power. The PCr half-time does not depend on the workload and represents a physiological invariant. The bioenergetic model was created during a long historical period, when it was believed that the La production was not present at all for very low exercise levels but, actually, the bioenergetic model predicts exactly the opposite result!

Anaerobic Threshold↗