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PubMed1987

Effect of lasalocid on weight gains, ruminal fermentation and forage intake of stocker cattle grazing winter wheat pasture.

Fifty fall-weaned heifers with initial weights of 209 kg (yr 1) and 222 kg (yr 2) were used to determine effects of lasalocid on weight gains, forage intake and ruminal fermentation of stocker cattle grazing winter wheat pasture. The heifers grazed a single wheat pasture for about 100 d each year, and were individually fed 1.06 kg of supplement (6 d/wk) pro-rated to supply 0, 100 or 200 mg lasalocid.head-1.d-1. Also, eight mature Hereford steers with large rumen cannula were used to evaluate further effects of lasalocid (0 or 300 mg) on ruminal fermentation during two grazing periods (immature and mature wheat forage) of yr 2 and an additional third year. Daily gains of heifers fed 200 mg lasalocid/d were .11 kg greater (P less than .05) than those of heifers fed 0 or 100 mg lasalocid/d. One hundred milligrams lasalocid did not increase weight gains. Digestibilities of forage dry matter (DM) and organic matter (OM) were similar (P greater than .05) among treatments, and lasalocid did not affect (P greater than .10) forage intake. Ruminal ammonia concentrations (10.57, 15.22 and 17.81 mg/dl +/- 1.71) were increased (P less than .05) by both levels of lasalocid in yr 1, but differences among treatment means of 8.32, 11.95 and 11.66 (SE +/- 1.44) were not significant in yr 2. Lasalocid did not consistently affect total volatile fatty acids concentrations. The acetic:propionic acid ratios in heifers were not different (P greater than .05) among treatments, but were decreased (P less than .10) by lasalocid in cannulated steers.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗
PubMed1988

Effects of forage source on ruminal microbial nitrogen metabolism and carbohydrate digestion in continuous culture.

Eight single-flow, continuous culture fermentors were used in Exp. 1 to study the effects of forage source on ruminal bacterial N metabolism and carbohydrate digestion. Forages included alfalfa, cicer milkvetch, birdsfoot trefoil and sainfoin with respective CP concentrations of 26.0, 28.7, 26.3 and 20.0%. Each forage provided 100% of the substrate for microbial metabolism and supplied 2.6 g N/d. Ammonia-N, protein degradation and efficiency of ruminal bacterial protein synthesis were lowest (P less than .05) for sainfoin. Protein degradation and efficiency of bacterial protein synthesis were higher (P less than .05) for birdsfoot trefoil than for alfalfa. Effluent flow of amino acids was highest (P less than .05) for sainfoin. Total nonstructural carbohydrate digestion tended to be highest for sainfoin and birdsfoot trefoil, whereas structural carbohydrate digestion was highest (P less than .05) for alfalfa and cicer milkvetch. In Exp. 2, mixed diets were supplied to dual-flow, continuous culture fermentors with alfalfa, cicer milkvetch, birdsfoot trefoil and sainfoin contributing 85% of the total dietary CP. Each diet contained approximately 12.9% CP. Ammonia-N concentration in the effluent and CP degradation tended to be lowest with the sainfoin diet and highest with the birdsfoot trefoil diet. Effluent flow of amino acids tended to be highest with the cicer milkvetch diet and lowest with the alfalfa and birdsfoot trefoil diet. Total structural and nonstructural carbohydrate digestion was not different (P greater than .05) among forages. Results from these experiments indicate that bacterial degradation of protein was lower for sainfoin than for alfalfa. Birdsfoot trefoil and cicer milkvetch appear to be comparable to alfalfa with regard to metabolism of N and carbohydrates by ruminal bacteria.

Animal Feed↗
PubMed2000

Performance of lactating dairy cows fed alfalfa or red clover silage as the sole forage.

Three Latin square trials, with 20 (two trials) or 24 (one trial) multiparous lactating Holstein cows (four in each trial with ruminal cannulae), compared the feeding value of red clover and alfalfa silages harvested over 3 yr. Overall, the forages contained similar amounts of neutral detergent fiber and acid detergent fiber; however, red clover silage contained more hemicellulose, less ash and crude protein (CP), and only 67% as much nonprotein N, as a proportion of total N, as did alfalfa silage. Diets were formulated with equal dry matter (DM) from alfalfa or red clover silage and contained on average 65% forage, 33 or 30% ground high moisture ear corn, and 0 or 3% low soluble fishmeal (DM basis). Diets fed in the Latin squares contained (mean dietary CP): 1) alfalfa (17.8% CP); 2) red clover (15.1% CP); 3) alfalfa plus fishmeal (19.6% CP); and 4) red clover plus fishmeal (16.9% CP). Although performance varied somewhat among trials, overall statistical analysis showed that replacing alfalfa with red clover reduced yields of milk, fat-corrected milk, fat, protein, lactose, and SNF; these effects were related to the 1.2 kg/d lower DM intake for cows fed red clover. Replacing alfalfa with red clover improved body weight gain and reduced concentrations of milk and blood urea and ruminal NH3. Apparent digestibility of DM, organic matter, neutral detergent fiber, acid detergent fiber, and hemicellulose all were greater when red clover was fed. There were no significant forage x fishmeal interactions for DM intake and yield of milk and milk components, indicating that supplementation with rumen undegradable protein gave similar increases in production on both forages. Net energy of lactation (NE(L)), estimated from maintenance, mean milk yield, and body weight change, in alfalfa and red clover silage were, respectively, 1.25 and 1.38 Mcal NE(L)/kg of DM, indicating 10% greater NE(L) in red clover.

Animal Feed↗
PubMed2002

Lactation performance by dairy cows fed wet brewers grains or whole cottonseed to replace forage.

Holstein cows starting at wk 8 of lactation were used to evaluate lactation performance when wet brewers grains, whole linted cottonseed, or starch-coated whole linted cottonseed (Easiflo) were substituted for forage. The wet brewers grains were added to diets to decrease forage neutral detergent fiber from 21% incrementally down to 15% while simultaneously decreasing nonfiber carbohydrate concentration from 40.3% down to about 33.8%. The cottonseed treatments all had similar concentrations of forage neutral detergent fiber (15%) and nonfiber carbohydrates (33.1 and 36.0%). Dry matter intake and milk production were similar across treatments. Milk fat percentage was decreased for Easiflo versus whole linted cottonseeds, but no other responses were detected. The current National Research Council (NRC) energy model was evaluated using individual cow data that were averaged over the entire 16-wk treatment period. For treatment means, the output of energy averaged 99% of the net energy of lactation intake, indicating very good corroboration of the model to account for energy usage for a group of cows. However, the ability to predict energy usage for individual cows was less accurate based on the comparison of residuals of observed and predicted body weight change regressed against predicted body weight change, apparently because of compounding of random errors in this prediction, which was alleviated over a larger number of observations. These results also corroborate current NRC guidelines for minimum forage neutral detergent fiber concentrations for lactating cows past the calving transition period.

Animal Feed↗
PubMed2002

Forage intake, meal patterns, and milk production of lactating dairy cows fed grass silage or pea-wheat bi-crop silages.

This study investigated the feed intake, milk production, and plasma nutrient status in dairy cows fed inter-cropped pea-wheat (bi-crop) silages comprised of contrasting ratios of pea to wheat. Spring peas (cv. Magnus) and wheat (cv. Axona) sown at either high (75:25) or low (25:75) pea inclusion rates were harvested after 13 (Cut 1) or 15 (Cut 2) wk. Eighteen Holstein-Friesian cows between wk 9 and 10 of lactation were used in a cyclical changeover design with three 28-d periods. Cows were fed the bi-crop silages and 6 kg of concentrates or second-cut grass silage supplemented with 6 (GS6) or 9 (GS9) kg/d of concentrates. Forage intakes were higher when bi-crops were fed (10.3 to 11.4 kg dry matter [DM]/d) than when grass silage was fed (8.6 kg DM/d). Total DM intake was similar among cows fed the bi-crop silages and GS9 diets, but intakes for GS6 were at least 1.7 kg DM/d lower. Increasing the pea inclusion rate increased the crude protein (CP) content of the ration, but it did not enhance forage quality or animal performance. The rate of intake of the different forages was similar, so that the higher intakes of bi-crop silages were associated with more time spent at the feedbunk and an increased number of meals. Diet digestibility ranged from 531 to 650 g/kg, and the highest value was given by the Cut 1 bi-crop silage diet. Milk yield tended to be similar for cows fed the Cut 2 bi-crop and GS9 diets, and these values were at least 1.7 kg higher than those for cows fed on other treatments. Generally, the bi-crop diets resulted in higher milk fat contents and lower polyunsaturated fatty acid contents. Milk protein content was highest for cows fed the GS9 diet. Blood metabolite content was unaffected by treatment except for blood urea nitrogen content, which was higher in cows fed the bi-crop silages, reflecting reduced N-use efficiency with these diets. The study showed that pea-wheat bi-crop silages can be used to replace moderate-quality grass silage in dairy cow rations, but their role as alternatives to high-quality forages requires additional investigation.

Animal Nutritional Physiological Phenomena↗
PubMed2004

Comparison of brown midrib-6 and -18 forage sorghum with conventional sorghum and corn silage in diets of lactating dairy cows.

Total mixed rations containing conventional forage sorghum, brown midrib (bmr)-6 forage sorghum, bmr-18 forage sorghum, or corn silage were fed to Holstein dairy cows to determine the effect on lactation, ruminal fermentation, and total tract nutrient digestion. Sixteen multiparous cows (4 ruminally fistulated; 124 d in milk) were assigned to 1 of 4 diets in a replicated Latin square design with 4-wk periods (21-d adaptation and 7 d of collection). Diets consisted of 40% test silage, 10% alfalfa silage, and 50% concentrate mix (dry basis). Acid detergent lignin concentration was reduced by 21 and 13%, respectively, for the bmr-6 and bmr-18 sorghum silages when compared with the conventional sorghum. Dry matter intake was not affected by diet. Production of 4% fat-corrected milk was greatest for cows fed bmr-6 (33.7 kg/d) and corn silage (33.3 kg/d), was least for cows fed the conventional sorghum (29.1 kg/d), and was intermediate for cows fed the bmr-18 sorghum (31.2 kg/d), which did not differ from any other diet. Total tract neutral detergent fiber (NDF) digestibility was greatest for the bmr-6 sorghum (54.4%) and corn silage (54.1%) diets and was lower for the conventional (40.8%) and bmr-18 sorghum (47.9%) diets. In situ extent of NDF digestion was greatest for the bmr-6 sorghum (76.4%) and corn silage (79.0%) diets, least for the conventional sorghum diet (70.4%), and intermediate for the bmr-18 sorghum silage diet (73.1%), which was not different from the other diets. Results of this study indicate that the bmr-6 sorghum hybrid outperformed the conventional sorghum hybrid; the bmr-18 sorghum was intermediate between conventional and bmr-6 in most cases. Additionally, the bmr-6 hybrid resulted in lactational performance equivalent to the corn hybrid used in this study. There are important compositional differences among bmr forage sorghum hybrids that need to be characterized to predict animal response accurately.

Animal Nutritional Physiological Phenomena↗
PubMed2006

Effect of forage:concentrate ratio on fatty acid composition of rumen bacteria isolated from ruminal and duodenal digesta.

Four dairy cows were used to examine the effect of the dietary forage:concentrate ratio [35:65, 50:50, 65:35, and 80:20 on a dry matter (DM) basis] on the fatty acid composition of rumen bacteria isolated from the liquid (LAB) and solid (SAB) phase of the rumen and duodenal digesta. Rumen contents were sampled 4 h after the morning feeding. Solid and liquid phases were separated from rumen contents and duodenal bacteria from a composite duodenal sample by differential centrifugation. Total fatty acid content in bacterial DM was 1.6 to 2.8 times higher in SAB compared with LAB, and increased with dietary concentrate. In combination with published reports, the data show that bacterial fatty acid content and composition is closely related to dietary fatty acids except for C18:2n-6 and C18:3n-3. A decrease in forage:concentrate ratio increased bacterial concentration of trans-10 C18:1, and this increase was 3.4 times higher in LAB compared with SAB. Analysis of odd- and branched-chain fatty acids showed large differences between SAB and LAB, which probably reflected a difference in species composition. The variation in odd- and branched-chain fatty acids between SAB and LAB was used to estimate their relative proportions in duodenal bacteria by means of linear programming, and showed an increased proportion of SAB from 64.7 to 74.8% with increasing forage:concentrate ratio. In addition, increasing the proportion of dietary forage was closely related to the proportion of anteiso C15:0 in total odd- and branched-chain fatty acids (r(pearson) = -0.771). The bacterial concentration of iso C17:0 closely reflected the bacterial growth rate as shown by the relation with cytosine:N (r(pearson) = -0.729). These strong relationships suggest that odd- and branched-chain fatty acids might be used as tool to evaluate nutrient supply to rumen bacteria.

Animal Feed↗
PubMed1976

Response of crickets to amount of forage in the diet.

Three replicated tests were to define the conditions needed to use house crickets (Acheta domesticus) to evaluate forages. Crickets were placed on various proportion of forage-to-concentrate mixtures to determine the relative proportions best suited for cricket survival, growth, and maturity. The concentrate portion alone was adequate to support nominal cricket performance. Forages used were timothy, alfalfa, and alfalfatimothy (1:1). Forages replaced from 0 to 100%, in increments of 10% of the concentrate portion. A diet of 40% concentrate and 60% roughage was suitable for evaluating growth and survival effects. The 28-day tests were not long enough to evaluate fully the effects of diet on maturity.

Animal Feed↗
PubMed1980

All silage forage programs for dairy cattle. III. Health, survival, and reproduction.

Holstein-Friesian heifer calves were fed roughage consisting of corn silage only, corn silage and haycrop silage (60:40, dry matter), or corn silage and dry hay (60:40 dry matter) from birth through completion of three lactations. Forages were fed ad libitum throughout and supplemented as required. Objectives were to determine effects of forage on survival, health, and reproduction. Of the 15 calves allotted per treatment 6, 10, and 7 completed three lactations fed corn silage, corn silage plus haycrop silage, or corn silage plus hay. At any time fewer animals survived on corn silage treatment resulting in 22 completed lactations compared to 36 and 29 lactations for those receiving haycrop silage or hay. Primary reasons for removal from experiment were enzootic pneumonia, 7; reproductive failure, 8; and mastitis, 6; and not forage treatment. Fewer animals on corn silage treatment were primarily from calfhood pneumonia. Reproduction measured by age at first calving (24.6 mo), calving interval (398 days), and services per conception (2.1) was similar across treatments. Incidence of health problems was in normal ranges and similar across treatments. Forage treatment had no effect on incidence of ketosis, left displaced abomasum, or simple indigestion.

Animal Feed↗
PubMed1991

Effect of source and amount of fiber on kinetics of digestion and specific gravity of forage particles in the rumen.

This experiment investigated the relationship between kinetics of digestion and change in specific gravity during in situ incubation. Nine cows were fed three sources of fiber (corn silage, alfalfa silage, or alfalfa hay) in diets formulated to contain 25, 30, or 35% NDF in three simultaneous 3 x 3 Latin squares. Method of alfalfa preservation did not influence rate of digestion or rate of increase in specific gravity of forage particles measured by a flotation technique. Prior to incubation, specific gravity of forage particles was in increasing order: alfalfa hay, alfalfa silage, and then corn silage. Essentially, all particles with a specific gravity less than 1.0 shifted to a higher specific gravity fraction by hydration within the first 4 h of incubation. From 4 to 56 h of incubation, percentage of residual DM that settled in solution having specific gravity of 1.3 increased linearly from 21 to 27% for corn silage but exponentially from 3 to 20% for alfalfa forages. Fractional rates of DM and NDF digestion and increase in percentage of residual DM having a specific gravity greater than 1.3 increased with the amount of fiber in the alfalfa diets and were correlated positively, suggesting that rate of increase in specific gravity, which affects rate of passage from the rumen, is influenced by rate of digestion of forage particles.

Animal Feed↗
PubMed1991

Effect of forage source on retention of digesta markers applied to corn gluten meal and brewers grains for heifers.

Effect of forage source on retention time of dietary protein supplements in the rumen was measured, and solid and liquid markers of digesta passage were compared. Two markers for liquid, two for each of the forages, and two for each of the supplemental proteins were compared for estimating retention time. Eight Holstein heifers were allocated to a single-reversal design with two periods of 29 d each. Heifers were fed either alfalfa hay or corn husks and leaves combined with one of two resistant protein sources: corn gluten meal or wet brewers grains. Average rumen liquid retention times, measured with Co-EDTA or Cr-EDTA, were similar (16.2 to 16.9 h). Liquid retention times differed when alfalfa or corn residue was fed (14.1 and 18.9 h, respectively). Rumen retention times were similar for Sm and Ce (24.9 to 26.3 h) and across markers for forage sources (25.1 to 26.1 h). Total tract mean retention time was higher for corn residue than for hay (57.8 vs. 48.8 h). Rumen retention times for La and Yb (27.1 and 26.0 h) applied to protein sources were similar and were not different when applied to corn gluten meal or brewers grains (26.3 and 26.8 h). Forage source did not have an effect on the retention time of protein supplements. Low daily DMI of 2.22 and 1.91% of BW for hay and corn residue diets explain the rather long retention times of the protein supplements. Both Co-EDTA and Cr-EDTA were effective as liquid phase markers.

Animal Feed↗
PubMed1994

Influence of corn and sorghum starch on the in vitro kinetics of forage fiber digestion.

In vitro digestion experiments were conducted to examine the interactions of starch source and pH on kinetics and apparent extent of ruminal NDF digestion. Alfalfa hay, bromegrass hay, and each hay with either raw sorghum, raw corn, or pure corn starch in ratios to simulate a 28% NDF diet were incubated at pH 5.5, 6.2, or 6.8. Ash-free NDF was measured at 0, 6, 12, 18, 24, 30, 36, 48, 72, and 96 h of fermentation; logarithmic transformation and linear regression were used to estimate digestion parameters. Significant forage by starch interactions on lag and rate of NDF digestion and significant forage and starch by pH interactions on rate and potential extent of NDF digestion indicated that forage type must be considered when the effect of pH and starch source on NDF digestion is predicted. For both forages, raw or pure corn starch increased lag more than sorghum starch, especially at low pH. Raw sorghum starch decreased the rate of NDF digestion more for bromegrass hay than for alfalfa hay, and pure corn starch had the largest negative impact on fiber digestion, especially at low pH. Sources of starch influenced lag and rate of NDF digestion differently at pH from 6.8 to 5.5, which led to dramatic differences in apparent extent of ruminal NDF digestion.

Animal Feed↗
PubMed1995

Effect of dietary neutral detergent fiber concentration and forage source on performance of lactating cows.

'Mott' dwarf elephantgrass, forage sorghum, 'Tifton 81' bermudagrass, and whole corn plant were stored as silage and fed as the only forage source in diets formulated to 31, 35, and 39% NDF. The 12 diets were fed for ad libitum intake as a TMR to midlactation Holstein cows, primarily to determine the effect of NDF concentration and forage source on DMI and milk production. Increased dietary NDF concentration linearly decreased DMI (3.69 to 3.35%) and OM intake (3.39 to 3.06%) as a percentage of BW and linearly increased NDF intake as a percentage of BW (1.15 to 1.32%). Production of milk (23.0 to 21.7 kg/d) and 4% FCM (20.7 to 19.6 kg/d) and percentage of milk protein (3.16 to 3.07%) decreased linearly as dietary NDF concentration increased. Cows fed dwarf elephantgrass silage or corn silage, which were higher quality forage sources based on greater rates and extents of fiber digestion, consumed more DM and produced more milk than cows fed sorghum silage or bermudagrass silage. Cows fed diets containing sorghum silage had the lowest DMI and milk production. Apparent digestibility of NDF and ADF from corn silage and dwarf elephantgrass silage was greater than that of sorghum silage and bermudagrass silage. In addition to dietary NDF concentration, digestibility of dietary fiber influences DMI and milk production.

Analysis of Variance↗
PubMed1995

Effects of inoculation and wilting on the preservation and utilization of wheat forage.

Wheat forage was harvested at an early head stage of maturity and ensiled in 12 900-kg experimental silos at three percentages of DM (20.8% for direct-cut forage and 27.9 or 39.3% for wilted forage) either with or without application of a lactic acid bacterial inoculant. The objective was to test the efficacy of the inoculant to alter silage fermentation, preservation, and nutritive value of wheat forage ensiled at different moisture percentages because of wilting. Wilting enhanced DM preservation and decreased fermentation end products. Inoculation made the fermentation more homolactic but did not enhance DM preservation. Silage rations (80% DM as silage) were fed at 1.8% of BW/d to six ruminally and abomasally fistulated steers (350 kg) in an experiment with a Latin-square design and a 3 x 2 factorial arrangement of treatments. Digestive responses to silage diets were not influenced by inoculation. Intake was depressed with direct-cut silage rations. Wilting improved fiber digestibility and was associated with changes in ruminal contents and fermentation end products. Wilting appears to be more effective than inoculation as a postharvest management tool to improve small grain silage.

Animal Feed↗
PubMed1996

A simple method for the analysis of particle sizes of forage and total mixed rations.

A simple separator was developed to determine the particle sizes of forage and TMR that allows for easy separation of wet forage into three fractions and also allows plotting of the particle size distribution. The device was designed to mimic the laboratory-scale separator for forage particle sizes that was specified by Standard S424 of the American Society of Agricultural Engineers. A comparison of results using the standard device and the newly developed separator indicated no difference in ability to predict fractions of particles with maximum length of less than 8 and 19 mm. The separator requires a small quantity of sample (1.4 L) and is manually operated. The materials on the screens and bottom pan were weighed to obtain the cumulative percentage of sample that was undersize for the two fractions. The results were then plotted using the Weibull distribution, which proved to be the best fit for the data. Convenience samples of haycrop silage, corn silage, and TMR from farms in the northeastern US were analyzed using the forage and TMR separator, and the range of observed values are given.

Animal Feed↗
PubMed1997

Influence of level of concentrate allocation and fermentability of forage fiber on chewing behavior and production of dairy cows.

Nine midlactation dairy cows were offered one of three mixed silage rations with neutral detergent fiber (NDF) that was similar in concentration but different in fermentability. Differences in fermentability were achieved by substituting a high quality alfalfa silage for a low quality alfalfa silage and substituting a combination of ryegrass and timothy silages for a barley silage. In addition, concentrate was allocated at 0.30, 0.82, or 1.37 kg of dry matter/kg of dry matter intake (DMI) from the mixed silage ration. As expected, the NDF content of the mixed silage ration did not differ, although fermentability of NDF increased numerically as forage quality increased. Intake of NDF increased linearly, and DMI tended to increase linearly, as fiber fermentability of the mixed silage ration increased. In addition, cows produced more milk, milk fat, and milk protein and generated more total and milk energy. The calculated concentration of net energy for lactation of the total diet also increased. Results support the concept that NDF quality influences and can be used to predict voluntary feed intake, at least in relatively high producing dairy cows. The DMI increased, and intake of NDF and crude protein declined, as the allocation of concentrate increased. In addition, as concentrate allocation increased, cows spent less time eating and ruminating and more time resting and produced more milk, milk protein, and milk lactose. Cows also generated more total energy and milk energy, although, despite a sharp decrease in the forage proportion of the diet, the calculated energy density of the diet did not differ among concentrate levels. The lack of significant interactions between concentrate level and fiber fermentability for any parameter measured supports the contention that high quality forage is critical to a successful dairy ration, regardless of the proportion of forage in the diet.

Animal Feed↗
PubMed1998

Forage of different physical forms in the diets of lactating Granadina goats: nutrient digestibility and milk production and composition.

To determine whether the energy balance of goats or characteristics of the diet consumed were the principal factors that determined milk production, feeding and digestion trials were carried out using two groups of 5 Granadina goats. The concentrate fraction of both diets was the same, but the forage fraction of the diets differed. In diet 1, the forage was in the form of long alfalfa hay, and, in diet 2, forage was in the form of pelleted alfalfa. Intake and the forage to concentrate ratio of the two diets were not significantly different, although diet 2 was more digestible. The amount of fat and protein in the milk depended on energy intake and not on dietary treatment. The milk protein of goats fed diet 2 was higher in casein. No sensible differences were noted in the fatty acid composition of the milk. Nitrogen and metabolizable energy utilization for milk production was greater for goats fed diet 2. According to the results obtained, it would seem advantageous to use pelleted alfalfa rather than alfalfa hay in the diets of goats.

Animal Nutritional Physiological Phenomena↗
PubMedDate not supplied

Screening nitrate in forages with a test strip: collaborative study.

A collaborative study was conducted for screening nitrate in forages with a commercially available test strip. The method involves extracting a finely ground sample with deionized water. The test strip is dipped in the sample extract. The color of the reaction zone on the test strip changes from white to pink or purple depending on the nitrate concentration in sample extract. The nitrate present in the extract is determined by comparing the color of the test strip to the color scale on the test strip container. Six blind quintuplicates of forage samples were analyzed by 20 collaborators. Nitrate concentrations in forage samples tested ranged from < 1000 ppm nitrate to > 10,000 ppm nitrate on dry matter basis. Each collaborator was asked to assign each sample to one of the 4 following nitrate concentration ranges: (1) < 1000 ppm, (2) 1000 to 5000 ppm, (3) > 5000 ppm to 10,000 ppm, and (4) > 10,000 ppm. Nineteen of 20 collaborators reported results. Results from 2 laboratories were rejected as outliers by inspection and chi 2 test. Sensitivity rates (p+) ranged from 0.965 to 0.998, with standard errors of 0.006 to 0.16. Specificity rates (p-) ranged from 0.991 to 0.997 for the 4 ranges, with standard errors of 0.003 to 0.006. False-positive rates (pf+) ranged from 0.006 to 0.046, with standard errors of 0.006 to 0.025. False-negative rates (pf-) ranged from 0.003 to 0.007, with standard errors of 0.003 to 0.006. Screening nitrate in forages with a test strip has been adopted first action by AOAC INTERNATIONAL.

Animal Feed↗