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[Food-related fungal infection risk in agranulocytosis. Mycological control of 273 food items offered to patients hospitalized in sterile units].

In sterile hospital units filamentous fungal infections are an unacceptable risk in patients with prolonged agranulocytosis. The occurrence of 3 successive cases in the Sterile unit of University Hospital of Grenoble prompted us to investigate in all elements of sterile unit, and notably food-processing. Two cooked rice dishes given to these patients were found to contain a substantial amount of Aspergillus fumigatus and were probably responsible for these infections. A more detailed study was carried out to evaluate the degree of food contamination by fungi in sterile unit. The study included 97 cooked dishes which had been sterilized in a 250 degrees oven for 40 minutes and 176 industrially packed foods delivered in single packs (e.g. milk and its derivatives, sweets and drinks). Each foodstuff was mycologically examined 3 to 5 times in different packs according to a procedure used in food analysis laboratories and raised to the standards of mycological examination. In this way, a 3-monthly food testing protocol in sterile unit could be set up. This protocol has a dual purpose: regular checking of the cooked dishes chain and maximum diversification of the dishes.

Agranulocytosis↗

Comparison between HPLC and MALDI-TOF MS analysis of anthocyanins in highbush blueberries.

High-performance liquid chromatography (HPLC) has been widely used as a reliable technique to quantify anthocyanins in food samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a new technique that is having a great impact on food analysis. This study is the first to compare HPLC and MALDI-TOF MS quantifications of anthocyanins. The analyses were carried out for highbush blueberries at different stages of anthocyanin formation. In general, both techniques provided comparable quantitative anthocyanin profiles for the samples. HPLC could distinguish anthocyanin isomers, whereas MALDI-TOF MS proved to be more rapid in the accurate identification and quantification of anthocyanins with different masses. A single MALDI-TOF MS run took just 4 min. MALDI-TOF MS analysis can serve as a rapid alternative to HPLC for the analysis of anthocyanins in fruits.

Anthocyanins↗

Determination of chlorinated pesticide residues in foods. II. Simultaneous analysis of chlorinated pesticide and phthalate ester residues by using AgNO3-coated Florisil column chromatography for cleanup of various samples.

A simplified method suitable for simultaneous analysis of chlorinated pesticide and phthalate ester residues in various foods was developed. Chemical residues were quantitatively extracted from fatty and vegetable samples with acetonitrile as follows: Chemical standard in 0.5 mL ethanol solution was added to 10 g homogenized sample. After 3 hr, pork and beef were extracted 3 times with 20 mL portions of acetonitrile. The acetonitrile layers were diluted with water and extracted with n-hexane. Rice samples were combined with 10 mL water, 5 mL acetonitrile and 1 mL ethanol and extracted 3 times with 20 mL portions of n-hexane. The n-hexane concentrate from each sample was submitted to AgNO3-coated Florisil column chromatography. The AgNO3 coating adequately adsorbed interfering coextractives. Extracts of fish and vegetable samples were separated into 2 fractions by the above column chromatography. Supplemental cleanup procedures were also developed to accurately determine phthalate esters eluted in the second fraction. Satisfactory gas chromatograms were obtained for most samples.

Animals↗

Comparison of gastric emptying of a solid and a liquid nutritional rehabilitation food.

OBJECTIVE: To compare gastric emptying of a solid and a liquid nutritional rehabilitation food. DESIGN: Cross-over trial. Eight volunteers took, in random order at least 1 week apart, 2100 kJ of a solid and a liquid nutritional rehabilitation food with added labelled [13C] glycine. SETTING: Research ward for healthy volunteers. SUBJECTS: Eight healthy volunteers. METHODS: Appearance of 13CO2 was monitored in expired gas every 15 min for 240 min after meal intake and compared between solid and liquid meals. Parameters of gastric emptying were then calculated. RESULTS: Gastric half-emptying time was slightly longer for solid meal compared to liquid meal (101+/-6.0 vs 88+/-9.8 min, P=0.24). 13CO2 excretion peaked 138+/-5.3 min after the solid meal compared with 119+/-8.6 min with liquid meal (P=0.06). 13CO2 excretion was higher for the liquid food up to 110 min after the meal but then became higher for the solid food. Analysis of variance for repeated measures of 13CO2 excretion showed a significant interaction term between the type of food and time indicating a delayed elimination of 13CO2 for the solid food (P=0.018). CONCLUSIONS: Attempts to replace liquid nutritional rehabilitation foods by a solid food in malnourished patients should take into account a possible slower gastric emptying. SPONSORSHIP: Supported by a grant from Nutriset (Malaunay, France) and ANVAR (Agence Nationale de la Valorisation de la Recherche, contract no. A9703021P).

Adult↗

Application of hazard analysis critical control points (HACCP) to organic chemical contaminants in food.

Hazard Analysis Critical Control Points (HACCP) is a systematic approach to the identification, assessment, and control of hazards that was developed as an effective alternative to conventional end-point analysis to control food safety. It has been described as the most effective means of controlling foodborne diseases, and its application to the control of microbiological hazards has been accepted internationally. By contrast, relatively little has been reported relating to the potential use of HACCP, or HACCP-like procedures, to control chemical contaminants of food. This article presents an overview of the implementation of HACCP and discusses its application to the control of organic chemical contaminants in the food chain. Although this is likely to result in many of the advantages previously identified for microbiological HACCP, that is, more effective, efficient, and economical hazard management, a number of areas are identified that require further research and development. These include: (1) a need to refine the methods of chemical contaminant identification and risk assessment employed, (2) develop more cost-effective monitoring and control methods for routine chemical contaminant surveillance of food, and (3) improve the effectiveness of process optimization for the control of chemical contaminants in food.

Consumer Product Safety↗

Chromatographic analysis of unsaponifiable compounds of olive oils and fat-containing foods.

The analysis of the "minor components" present in food lipids is usually hampered by the large diversity of compounds found in this fraction. High-purity degree reagents and solvents, good collection techniques and highly sensitive analysis are required in order to accurately identify and quantify these components. Chromatographic techniques have proven to be particularly suitable for these determinations, especially capillary gas chromatography. This study reports several analytical cases of the main classes of components of the unsaponifiable matter obtained from olive oils or food matrices.

Chromatography↗

Food security and perceptions of health status: a preliminary study in rural Appalachia.

Food insecurity is estimated to affect about 10% of the United States population. Rural areas experience even higher rates and intensity of food security problems related to poverty, food access, and higher food costs. Reports of the relationship between household food security and health status, however, are limited. This report examines the relationship between household food security and measures of functional health status in a rural Appalachian sample. A comprehensive health status survey was completed by 1,006 individuals seen either in a clinical (n = 605) or nonclinical (n = 401) community setting. The survey included the USDA Food Security Core Module, the SF-36, and demographic and health care access questions. Household food insecurity was reported by 23% of respondents. Food insecure respondents reported significantly poorerfunctional status on all SF-36 scales compared tofood secure respondents (all p < 0.05). After adjusting for demographic and access variables in a multiple regression analysis, food insecurity remained a significant independent predictor of responses for each SF-36 scale. Generalizability of results are limited by the convenience sampling methods and geographic region in which the study was conducted. In this preliminary study, even minimal levels of food insecurity are related to self-reported levels of health status as measured by the SF-36 spectrum. Health professionals must be able to identify individuals at risk for food insufficiency; policy makers must develop more effective programs for alleviating the basic causes of food insecurity.

Adult↗

Rapid assessment of food/package interactions by electrochemical impedance spectroscopy (EIS).

Food components migrating through organic layers are able to influence the adhesive properties of multilayer materials. In the case of coated metal substrates the interaction stability may be reduced by delamination and subsequent corrosion processes during storage. Impedance spectroscopy can be used to give a very early indication of a reduced interaction stability of a packaging material by measuring the delamination tendency. Applications are presented for laminated aluminium foil material. The delamination is induced by a cathodic treatment of samples prepared from the package. A comparison is made of material with no contact and with only a few days contact with the filled product. Main applications are foreseen as the replacement of long-term storage tests to establish the compatibility of food and package material and to work out the specific influence of individual food components.

Aluminum↗

[Identification of unknown peaks in foods during residual pesticide analysis].

Four unknown peaks were detected in GC chromatograms during analysis of organophosphorus pesticide residues in foods. They were identified by using GC/MS as triethyl phosphate (TEP), tributyl phosphate (TBP), diphenyl 2-ethylhexyl phosphate (DPEHP) and N-ethyltoluenesulfoneamide (NETSA), which are used as plasticizers or flame retardants in food packaging. These chemical substances were detected in the range of tr. (below 0.01 microg/g) to 11 micro/g from 29 samples, and they were also detected in the packaging. It was supposed that they were transferred to the foods from the packaging. Furthermore, they were detected in some cereals and cereal products which contain fat and had been preserved for a long time.

Food Analysis↗

Analysis of natural coloring matters in food. III. Application of methylation with diazomethane for the detection of lac color.

A new method has been proposed for detection of lac color in food. Lac color is a natural color additive derived from a secretion of the insect Coccus Laccae (Laccifer lacca Kerr). It is extracted from food with methanolic oxalic acid and eluted from a column of Amberlite XAD-2 with the same solvent. The fraction containing the lac color is treated with diazomethane to produce 2 reddish-orange markers. The marker species in the reaction mixture are detected by both thin-layer chromatography and reverse-phase liquid chromatography.

Animals↗

New metrological characteristics of analytical methods of analysis used for safety control of food and environmental materials.

MQS and MQSm are new metrological characteristics of the limit of determination of methods used for safety control of foods and environmental objects. MQS is the minimum quantity of an analyte that can be determined by any applicable method in an analytical sample. MQSm is the minimum quantity of analyte that can be measured in the test solution in an interlaboratory, method-performance study of a specific method, under the condition that MQSm < or = MQS. Examples are given of their calculation and use.

Chemistry Techniques, Analytical↗

Analysis of natural food pigments by capillary electrophoresis.

Lac, cochineal, safflower, gardenia, Monascus and elderberry pigments are used as food color additives in Japan. These natural pigments can be analyzed by capillary electrophoresis (CE). CE has several advantages over thin layer chromatography, gas chromatography and high-performance liquid chromatography, such as low capillary cost, reduced operating costs, small sample amounts, low production of waste materials and short analysis time. CE is shown to be a useful technique for the analysis of these natural food pigments and the pigments extracted from commercial food samples by solid-phase extraction method.

Electrophoresis, Capillary↗

Prompting methods affect the accuracy of children's school lunch recalls.

OBJECTIVES: To evaluate the feasibility of implementing 3 specific prompting methods among students in the first and fourth grades (mean age = 7.2 and 10.1 years, respectively), to validate recall accuracy of first- and fourth-grade students against observation, to develop a single measure of inaccuracy that cumulated errors in reporting food items and amounts without allowing underreporting and overreporting to cancel each other, and to establish information regarding the measure of inaccuracy for use in future studies. DESIGN: Children were interviewed the morning after they were observed eating lunch provided by the school. Interviews included free recall, nonsuggestive prompted recall, and specific prompted recall (either preference, food category, or visual). SUBJECTS/SETTING: Ninety-six children (48 students per grade) were studied--32 per the specific prompting method--stratified by grade, sex, and ethnicity (African-American or white). Specific prompting method was randomly assigned to children within strata. STATISTICAL ANALYSIS: Food items were categorized and weighted as: combination entree = 2, condiments = 1/3, and other = 1. To calculate inaccuracy of recall, absolute differences between amounts reported and observed eaten were calculated for each item and then multiplied by each item's weight; these values were then summed across all items for each child. Inaccuracy of recall was determined before specific prompting and after specific prompting and for the difference (inaccuracy after specific prompting minus inaccuracy before specific prompting). RESULTS: Before specific prompting, median inaccuracy was 2.7 servings for the 48 first-grade students and 1.7 servings for the 48 fourth-grade students. The median difference in inaccuracy from before to after specific prompting was 0 for both grades. Specific prompting increased recall accuracy for 9 first-graders and 12 fourth-graders, but decreased recall accuracy for 21 first-graders and 7 fourth-graders, and did not change recall accuracy for 18 first-graders and 29 fourth-graders. Among children whose recall accuracy increased after specific prompting, 5 first-graders and 8 fourth-graders received prompting for food category. APPLICATIONS/CONCLUSIONS: Among first-grade students, specific prompting (either preference, food category, or visual) may hurt more than help recall accuracy. Among fourth-grade students, prompting for food category yields small gains in recall accuracy with minimal losses. Validation studies are needed with larger sample sizes to determine prompting methods that produce more accurate dietary recalls from children of various socioeconomic status groups, other racial/ethnic groups, and at other meals.

Analysis of Variance↗

Estimates of fruit and vegetable intake in childhood and adult dietary behaviors of African American women.

OBJECTIVE: This exploratory study examined how estimates of one's fruit and vegetable intake in childhood are related to 3 current dietary behaviors among African American women: intake of fruits and vegetables, exposure to and preference for fruits and vegetables, and preference for trying new foods. DESIGN: Baseline data from a randomized dietary intervention trial. SETTING: Ten urban public health centers in St. Louis, Missouri. PARTICIPANTS: 1227 African American women. VARIABLES MEASURED: A 33-item fruit and vegetable food frequency questionnaire, items measuring estimates of childhood fruit and vegetable intake, adult fruit and vegetable intake, exposure to and preference for fruit and vegetable, and preference for trying new foods. ANALYSIS: Linear regression evaluated the association between predictors and continuous measures; logistic regression determined the association between predictors and categorical measures. RESULTS: Estimates of one's vegetable intake as a child were significantly related to exposure and preference for both fruits and vegetables, trying of new foods, and intake of both fruits and vegetables in adulthood. Estimates of eating fruit as a child were not significantly associated with these adult dietary behaviors. CONCLUSIONS AND IMPLICATIONS: Developmental influences on adult dietary patterns may be stronger for vegetables than fruits among African American women. Additional emphasis is needed regarding exposure to and preference for vegetable intake in childhood.

Adolescent↗

Dietary intake of fluoride ashed (total fluoride) v. unashed (inorganic fluoride) analysis of individual foods.

Fluoride content in ninety-three individual food items from a hospital in a fluoridated area was determined by ashing (total fluoride) v. unashing (inorganic fluoride) analysis. No discrepancy between the two methods was found by food group but two dry cereals and black pepper did show significantly more fluoride after ashing. The reason for the unavailability before ashing was not determined. Daily fluoride intake was estimated at 1.783 mg which is midway between the 1.211 and 2.201 mg reported from studies in which composite diets were analysed. Daily intake from food at 0.4 mg was one-quarter of the daily total intake 1.8 mg; a ratio consistent with those previously reported in serum, urine and bone between residents from a non-fluoridated v. fluoridated community.

Diet↗