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[University by radio and television in Great Britain. The Open University].

There are some 20 years since Great Britain was given a new and important University (established by a Royal Charter in 1969), especially intended for students wanting to achieve their education at home. The whole teaching, guided by tutors, is supported by various materials: booklets, audio- and videotapes which are completed by television and radio broadcasts for many courses and informations. After successful examinations are obtained the usual degrees and certificates delivered by the British Universities. Furthermore, this kind of education begins to be available in a few other countries (Belgium, Luxembourg, The Netherlands, Cyprus, Hong Kong).

Audiovisual Aids↗

[Environmental protection techniques used in the federal state of Baden-Württemberg in Germany].

Within the framework of the programme "Partnership for Sustainable Development" The Ministry of Environment of the German Federal State of Baden-Württemberg has come up with a project "Study Visit--Environmental Protection Techniques". It was intended as a three-week study visit for environmental protection experts from Central and Eastern European Countries (CEEC) to learn about the environmental protection techniques used in this federal state. Visits were paid to companies producing, applying or installing plants based on the state-of-the-art environmental protection techniques. The project started in 2005 and will last five years. The first visit to Baden-Württemberg was scheduled for 25 September-14 October 2005 for 12 experts from 12 countries: Bulgaria, Croatia, Cyprus, Czech Republic, Estonia, Hungary, Latvia, Lithuania, Poland, Romania, Slovakia and Ukraine.

Conservation of Natural Resources↗

Clinical, physiological and serological observations of a human following a venomous bite by Macrovipera lebetina lebetina (Reptilia: Serpentes).

A researcher, a 40 year-old male, was bitten on his finger accidentally by a 75 cm long, adult male blunt-nosed viper, Macrovipera lebetina lebetina (Linnaeus) that had been collected from northern Cyprus during laboratory work in the Zoology Section, Biology Department of the Ege University Faculty of Science. The clinical signs, physiological and biochemical changes soon after the venomous bite by the blunt-nosed viper as well as the electrophoretic separation of plasma proteins of the patient have been presented here. Serious important symptoms (edema, hypotension shock, hemorrhage, tissue necrosis, and melanoderma) developed and major quantitative differences in the fractions of albumin, globulin and albumin/globulin ratios were detected. On the other hand, the measurements carried out in blood smears and biochemical results indicated the decrease in RBC, MCV, MHV and a decrease in the diameter of red blood cells, as well as an increase in WBC.

Adult↗

[History of opium poppy and morphine].

Opium has been known for millennia to relieve pain and its use for surgical analgesia has been recorded for several centuries. The Sumerian clay tablet (about 2100 BC) is considered to be the world's oldest recorded list of medical prescriptions. It is believed by some scholars that the opium poppy is referred to on the tablet. Some objects from the ancient Greek Minoan culture may also suggest the knowledge of the poppy. A goddess from about 1500 BC shows her hair adorned probably with poppy-capsules and her closed eyes disclose sedation. Also juglets probably imitating the poppy-capsules were found in that period in both Cyprus and Egypt. The first authentic reference to the milky juice of the poppy we find by Theophrastus at the beginning of the third century BC. In the first century the opium poppy and opium was known by Dioscorides, Pliny and Celsus and later on by Galen. Celsus suggests the use of opium before surgery and Dioscorides recommended patients should take mandrake (contains scopolamine and atropine) mixed with wine, before limb amputation. The Arabic physicians used opium very extensively and about 1000 AD it was recommended by Avicenna especially in diarrhoea and diseases of the eye. Polypharmacy, including a mixture of nonsensical medications were often used. Fortunately for both patients and physicians many of the preparations contained opium. The goal was a panacea for all diseases. A famous and expensive panacea was theriaca containing up to sixty drugs including opium. Simplified preparations of opium such as tinctura opii were used up to about 2000 in Denmark. In the early 1800s sciences developed and Sertürner isolated morphine from opium and was the founder of alkaloid research. A more safe and standardized effect was obtained by the pure opium. Several morphine-like drugs have been synthesized to minimize adverse effects and abuse potential. Opioid receptors were identified and characterized in binding assays and their localization examined. However, the complexity of the system including interaction with several neurons and transmitters indicate the goal of nonaddictive opiates to be elusive. Combination therapy, innovative delivery systems and long-acting formulations may improve clinical utility.

Ancient Lands↗

[Nurse's role in a program of alcoholism rehabilitation].

As far back as history goes alcoholism is known to have caused various problems: psychological, pathological and social. Theories regarding their causes are many and they vary in each case. In Cyprus, a special detoxification programme is being used, in which the nurse's role is very important. Special importance is accorded to the withdrawal symptoms in the nursing assessment and nursing care.

Alcoholism↗

Geographical and age-related variation in the activity of some enzymes associated with insecticide detoxification in sandflies (Diptera: Phlebotominae).

The activity of glutathione s-transferase (GST), glucose 6-phosphate dehydrogenase (G6PD), and esterases (EST1, EST2) was measured using microtitre plate assays of laboratory colonised Phlebotomus papatasi originating in Afghanistan, Cyprus, India, Iraq, Saudi Arabia, Spain and Tunisia. The mean enzyme activity of the seven populations was significantly different. Enzyme activity did not vary significantly with age in sandflies, unlike some mosquitoes. A baseline enzyme activity for susceptibility is proposed for P. papatasi.

Aging↗

Demonstration projects for the integrated prevention and control of noncommunicable diseases (INTERHEALTH programme): epidemiological background and rationale. INTERHEALTH Sterring Committee.

Noncommunicable diseases--cardiovascular and cerebrovascular disease, pulmonary diseases, liver disease, cancer, diabetes, osteoporosis and trauma--constitute the major cause of death in developed countries and are predictably emerging as significant threats to health in countries at intermediate stages of the epidemiological transition. Based on the philosophy that diseases with common risk factors (inadequate prevention/control services, smoking, fat/salt diet, alcohol use, etc.) require common preventive strategies, the INTERHEALTH demonstration projects are designed to build regional capacities and to exchange social and medical technologies for broad-gauged noncommunicable disease prevention and control. Projects are at various stages of planning and implementation in all WHO regions: Africa (Mauritius, United Republic of Tanzania); the Americas (Chile, Cuba, United States); Eastern Mediterranean (Cyprus); Europe (Finland, Malta, USSR); South-East Asia (Sri Lanka, Thailand); the Western Pacific (Australia, China, Fiji, Japan). This article presents selected data which illustrate the long-term mortality trends and present noncommunicable disease risk-factor levels in participating countries at different stages of the epidemiological transition. The shift towards noncommunicable diseases as a cause of death is readily apparent and combinations of risk factors are present in each of the populations studied in the baseline phase of this research and demonstration programme. The use of data to estimate the noncommunicable disease-related mortality burden from different lifestyles and risk factors is illustrated and findings from the most advanced demonstration studies are briefly outlined.

Global Health↗

Mild and severe beta-thalassemia among homozygotes from Turkey: identification of the types by hybridization of amplified DNA with synthetic probes.

Through the procedure of gene amplification combined with hybridization to synthetic 19 base pair (bp) oligonucleotide probes, it has been possible to identify nine different mutations in the DNA of 47 subjects from Turkey and Northern Cyprus with a beta-thalassemia homozygosity. The IVS-I nucleotide (nt) 110 G----A and the IVS-I nt 6 T----C substitutions and the frameshift at codon 8 were most frequently observed. Direct correlations were made between these data and clinical observations; mild disease was associated with homozygosity for IVS-I nt 6 T----C, for frameshift at codon 8, for the C----G substitution at nt -87, and for IVS-I nt 5 G----T, and for a double heterozygosity for some of these conditions. Moderate disease, observed in some of the patients, could be explained by combinations of specific mutations. All mutations were associated with specific haplotypes, while in some the observed beta zero-thalassemia was of the mild type due to a considerable production of Hb F.

DNA↗

[Micropreparative thin-layer peptide maps in the molecular diagnosis of abnormal human hemoglobins].

General factors determining the possibility of application of peptide maps in thin layer of microcrystalline cellulose as a micropreparative method in molecular diagnostics of abnormal hemoglobins were studied. The effects of absorbtional capacity of cellulose and amino acid impurities in it, choice of eluent and elution technique, peptide structure and extent of its modification in staining as well as completeness and specificity of globin chain enzymatic digestion on peptides extraction from thin layer were analysed. The results of structural identification of Hb D Punjab beta 121 Glu----Gln at a Cypriot; Hb O Arab; beta 121 Glu----Lys at a Bulgarian woman, living in Kalinin region (the second case in the USSR); Hb M Saskatoon beta 63 His----Tyr at a woman from Georgia (the second case in the USSR); Hb Buenos Aires beta 85 Phe----Ser at a Russian girl (the first case in the USSR and the third case in the world); Hb Dagestan alpha 60 Lys----Glu at two members of a Lesgin family from Dagestan; Hb Agenogi beta 90 Glu----Lys at a Hungarian woman; Hb Setif alpha 94 Asp----Tyr at three patients from Cyprus and Hb Detroit beta 95 Lys----Asn at an Azerbaijanian woman (the first case in the USSR and the second case in the world) are presented.

Amino Acids↗

High frequency of K15 antigen in enterotoxigenic Escherichia coli: lack of correlation to adherence to epithelial cells or sensitivity to bactericidal effect of normal serum.

Typing of E. coli O and K antigens, testing of sensitivity to bactericidal effect of normal serum and adherence to uroepithelial as well as buccal epithelial cells was performed in enterotoxinogenic (ETEC) and normal E. coli strains. The 99 ETEC faecal strains were obtained from Swedish and Ethiopian children and adults and Swedish soldiers in Cyprus with diarrhoea. The reference faecal strains were isolated from 120 healthy Swedish children. E. coli K15 was significantly more often seen in ETEC strains than in the reference strains, always in combination with O6 antigen. K15 is hardly ever seen in nonenterotoxinogenic strains. There was no significant difference in sensitivity to bactericidal effect of serum or adherence to epithelial cells between ETEC strains and normal strains. The epidemiological significance of the findings is discussed.

Adult↗

Studies on bluetongue. VI. Animal transmission studies.

The Cyprus strain of bluetongue virus was successfully transmitted through six passages and the Station strain through one passage in calves. Although the animals developed no visible evidence of infection, viremia as shown by both passage and fluorescent antibody examination of infected foetal bovine kidney culture, and by serological conversion was nevertheless demonstrated. No enhancement of virulence for calves or sheep was shown following bovine passage. A ewe inoculated in late pregnancy with blood drawn from a calf 59 days after its infection, gave birth to a lamb from whose blood the virus was isolated. Significant complement-fixation titres persisted for at least 200 days.

Animals↗

Prevention and control of haemoglobinopathies.

In many developing countries the haemoglobinopathies (thalassaemias and sickle-cell disorder) are so common that they provide a convenient model for working out a genetic approach to control of chronic childhood diseases. At present, about 250 million people (4.5% of the world population) carry a potentially pathological haemoglobinopathy gene. Each year about 300,000 infants are born with major haemoglobinopathies. Haemoglobinopathy control programmes, based on WHO approaches and recommendations, have been established in different countries in all WHO Regions and have been successful in management of the problem. Following WHO recommendations the health burden of hereditary anaemias could be significantly reduced. This article summarizes the presentations and discussion made at a joint WHO/TIF (Thalassaemia International Federation) meeting, held in Cyprus in April 1993, and reviews the experience of programmes in several countries for the control of haemoglobinopathies in the world.

Global Health↗

Thermal strain and G protection associated with wearing an enhanced anti-G protection system in a warm climate.

A flight trial was conducted in Cyprus to assess the thermal strain associated with and the G protection provided by the prototype Eurofighter 2000 aircrew equipment assembly (AEA) in a warm climate. Six subjects flew a standardized sortie four times in a Hawk aircraft: two while wearing the Eurofighter 2000 AEA and two wearing standard Hawk summer AEA. The sortie included high-G turns and simulated air combat. Cockpit temperatures, rectal and skin temperatures, heart rate, and sweat rate were recorded. Subjective thermal comfort, fatigue, and G protection were also assessed. Skin temperatures of the back, chest and thighs, mean skin temperatures, and sweat rate were greater when the Eurofighter AEA was worn. Rectal temperature and heart rate did not differ significantly between the two conditions. Superior G protection was provided by the Eurofighter assembly. These findings suggest that wearing the Eurofighter AEA in a warm climate is associated with an increased but not unacceptable level of thermal stress while offering enhanced G protection. These results may not generalize when ambient temperatures are higher or more insulative protective clothing is worn.

Adult↗

Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes.

The serogroup specificity of the bluetongue virus (BTV) NS1 and VP3 gene probes was confirmed by means of northern blot hybridization. Under high-stringency conditions both probes hybridized to 22 BTV serotypes (18 South African serotypes, BTV3 from Cyprus and BTV16 from Pakistan) but not to serotypes that originate from Australia and India. Furthermore, NS1 gene probes of BTV and African horsesickness virus (AHSV) were used in a dot-spot in situ hybridization procedure to differentiate between BTV and AHSV in co-infected cell cultures. The method detects viral RNA directly i glutaraldehyde-fixed infected cell cultures without prior nucleic-acid extraction or purification. AHSV could be detected in cells infected with AHSV at a multiplicity of infection of 10(-4) PFU/cell in the presence of a hundred excess of co-infecting BTV. The method may have an application in epidemiological surveys to detect different orbiviruses in the same Culicoides population.

African Horse Sickness Virus↗

[New aero-allergens].

As the number of proteins recognized as causing allergic respiratory diseases increases, new aero allergens have appeared in the animal and vegetable realms, both in home and professional environments. Lepidoglyphus destructor and Blomia tropicalis, two mites found in storage areas, are particularly important in agricultural areas and in homes. Over the last ten years, the frequency of reactions to cockroaches has also increased in several countries. The allergenicity of non-biting insects is a frequent cause of allergy in certain countries including Japan. Chironomides cause respiratory diseases in professional and outdoor environments. The important role of Alternaria, a mold, in producing severe asthma has also been demonstrated. The pathophysiology of pollen-induced asthma has been shown to result from pollen allergens carried by particles less than 5 microns in diameter. Cyprus and ash tree pollen also cause an increasing number of pollinoses and flowers can cause rhinitis and asthma. Respiratory allergy to Ficus benjamina inaugurated a new type of allergies caused airborne allergens from non-pollinating plants. Allergy to latex raises a particular problem for health care workers. The immunochemical structures of the major and minor airborne allergens are now better known and the homologous structures of different allergens largely explains certain cross-reactions. In the future, recombinant allergens will probably be used to better understand the role of allergens in inducing and maintaining the allergic reaction and should help in our approach to diagnosis and therapy.

Acari↗

[The European mouflon, Ovis musimon].

Until a couple of decades ago, the European mouflon found on the islands of Corsica, Sardinia, and Cyprus was considered an independent wild species and last representative of the European wild sheep. However, recent research has shown that there have not been wild sheep in Europe since the late Pleistocene. Archeological investigation in Corsica has shown that the mouflon is not a wild sheep but a primitive domestic sheep brought to the islands by farmers from the Near East and which then became wild (feral).

Animals↗

Legislative approaches to the regulation of the chiropractic profession.

Traditional and complementary health care services have a growing and significant role in both developed and developing countries. In the United Kingdom the British Medical Association (BMA) has identified five complementary approaches to health care that should now be regarded as "discrete clinical disciplines" because they have "established foundations of training and have the potential for greatest use alongside orthodox medical care". These are acupuncture, chiropractic, herbalism, homeopathy and osteopathy. The BMA recommended that there should be legislation to regulate these disciplines and the Chiropractors' Act enacted in the U.K in 1994. The chiropractic profession was founded in the United States in 1895, and the practice of chiropractic has been regulated in the United States and Canada since the 1920s, in Australia since the late 1940s, in New Zealand and South Africa since the 1960s, and more recently in Asia, Europe, Latin America and elsewhere. Figure 1 lists the countries which currently recognize and regulate the chiropractic profession. Many countries, such as Japan with approximately 10,000 chiropractors with different levels of education, and Trinidad & Tobago with 5 chiropractors who are graduates of accredited chiropractic colleges in North America, are considering legislation. Croatia, with 3 chiropractors, is preparing legislation. Cyprus, with 6 chiropractors, has legislation. Even in countries such as these, where the profession is small, there are compelling public interest arguments for regulation. This is especially true in the 1990s. One reason is the growing incentive for lay healers and others without formal training to use the title "chiropractor" as chiropractic practice gains increasing acceptance. The majority of chiropractic practice involves patients with non- specific or mechanical back and neck pain. The chiropractic approach to management, which includes spinal adjustment or manipulation, other physical treatments, postural advice, rehabilitative exercises and early return to activities, formally only had empirical evidence of success. Now there is firm scientific support. Recent national, evidence- based, multi-disciplinary guidelines in Canada (neck pain), the United Kingdom (back pain), and the United States (back pain) support these methods as a first line of management for most patients. Another reason for regulation is that international standards of chiropractic education and scope of practice have been established by appropriate chiropractic organizations, including the World Federation of Chiropractic which represents national associations of chiropractors in 63 countries. This paper now reviews current legislation worldwide.

Back Pain↗

Selenium content of sulfide ores related to ophiolites of Greece.

Several deposits of sulfide mineralization have been described in the ophiolites of Greece. Based on their mineralogical and chemical composition and the host rocks, two types can be distinguished: (1) the Fe-Cu-Ni-Co type consisting of pyrrhotite, chalcopyrite, Co-pentlandite, pyrite, magnetite + arsenides, +/- chromite, hosted in serpentinites, gabbros or diabases, which have variable geochemical characteristics, and (2) sulfide mineralization of the Cyprus type containing variable proportions of pyrite, chalcopyrite, bornite, and sphalerite. The spatial association with shear zones and fault systems, which is a common feature in both types of mineralization, provided the necessary permeability for the circulation of the responsible mineralized hydrothermal fluids. The selenium (Se) content in representative samples of both types of mineralization from the ophiolites of Pindos (Kondro, Perivoli, and Neropriona), Othrys (Eretria and A. Theodoroi), Veria (Trilofon), and Argolis (Ermioni) shows a wide variation. The highest values of Se (130 to 1900 ppm) were found in massive Fe-Cu sulfide ores from Kondro, in particular the Cu-rich portions (average 1300 ppm Se). The average values of Se for the Othrys sulfides are low (< 40 ppm Se). The Se content in a diabase breccia pipe (50 x 200 m) with disseminated pyrite mineralization (Neropriona) ranges from < 1 to 35 ppm Se. The highest values were noted in strongly altered samples that also exhibited a significant enrichment in platinum (1 ppm Pt). Sulfide mineralization (irregular to lens-like masses and stringers) associated with magnetite, hosted in gabbros exposed in the Perivoli area (Tsouma hill), shows a content ranging from 40 to 350 ppm Se. The distribution of Se in the studied type of the sulfide mineralization may be of genetic significance, indicating that the Se level, which often is much higher than in typical magmatic sulfides related to mafic-ultramafic rocks (average 90-100 ppm Se), may positively affect the environment.

Environment↗