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PubMed1977

Fine structure of the first optic ganglion (lamina) of the cockroach, Periplaneta americana.

The stuctural organization of the first optic ganglion (lamina) of the cockroach (Periplaneta americana) was investigated by the use of light and electron microscopy. Each compound eye of the cockroach is composed of up to 2000 visual units (ommatidia) of the fused rhabdom type. The ommatidia themselves consist of eight receptor cells which terminate as axons in either the first or second optic ganglion. Three different short visual fibre types end in two separate strata in the lamina, and one long fibre type ends in the second optic ganglion. Monopolar second-order neurons with wide field branching patterns in the middle stratum of the first synaptic region have postsynaptic contacts with sort visual fibres. Horizontal fibre elements with branching patterns at different levels of the lamina apparently from three horizontal plexuses with presynaptic and/or postsynaptic connections to first-and second-order neurons. The lack of well-organized fibre cartridges containing a constant number of first and second order neurons in each fascicle and the presence of only unistratified wide field monopolar cells could represent, as compared to other insect orders, a primitive stage in the development of the first optic ganglion.

Animals↗
PubMed1992

A bioassay for cobra cardiotoxin activity using semi-isolated cockroach heart.

A semi-isolated cockroach heart preparation was used to rapidly determine the activity of cobra cardiotoxin, monitored as a direct response on heart rate. This preparation produced a dose-response curve in the presence of active cardiotoxin and demonstrated that cardiotoxin retained its biological activity after boiling, although cardiotoxin activity was destroyed by heating in the presence of dithiothreitol. Experiments that cross-linked radiolabeled cardiotoxin to solubilized cockroach heart membranes suggested that cardiotoxin bound specifically to a 59,000 mol. wt membrane protein in this tissue.

Animals↗
PubMed1984

Effects of pre-training administration of scopolamine on learning and retention in the cockroach, P. americana.

The muscarinic cholinergic blocker, scopolamine, has no effect on acquisition or retention of the choice behavior of the cockroach, P. americana trained to turn right or left to avoid shock in a T-maze. Scopolamine does, however, prevent runway habituation during training, suggesting that such habituation may be dependent on central cholinergic synapses as there are no known peripheral cholinergic receptors in cockroaches. Previous work demonstrating that puromycin interferes with correct choice retention five hours after training taken together with our results demonstrating no effect of scopolamine on such retention, suggests that puromycin does not produce its amnesic effect on choice behavior by interferring with central cholinergic synaptic processes.

Animals↗
PubMed1988

Cockroach hypersensitivity: isolation and partial characterization of major allergens.

Two allergenic fractions in crude American cockroach extract have been isolated with Sephadex G-150 SF gel filtration and diethylaminoethylcellulose ion exchange chromatography. The partially purified fractions of Cr-PI and Cr-PII elicit 73% and 93% of patients sensitive to the crude extracts, respectively. Crude extract contains at least 29 antigenic components, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis. Eighteen allergenic arcs are identified by crossed radioimmunoelectrophoresis. Electroblotting analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals nine allergenic components of molecular weights of 120,000, 110,000, 90,000, 78,000, 72,000, 49,000, 45,000, 29,000, and 26,000 daltons that are able to bind specific IgE. Among these nine principal allergens, two components of molecular weights 78,000 and 72,000 daltons bound 100% of the sera of atopic patients tested. These two proteins are undoubtedly the major allergens of American cockroach extract.

Allergens↗
PubMed1990

Structure of the hypertrehalosemic neuropeptide of the German cockroach, Blattella germanica.

The structure of the hypertrehalosemic neuropeptide of the German cockroach, Blattella germanica, was found to be identical to that of the cockroach species, Blaberus discoidalis and Nauphoeta cinerea (Glp-Val-Asn-Phe-Ser-Pro-Gly-Trp-Gly-Thr-NH2). Since Blattella germanica is not closely related to Blaberus discoidalis and Nauphoeta cinerea, this supports the hypothesis that in this peptide family evolution of peptide structure may be related to evolution of peptide function.

Amino Acid Sequence↗
PubMed1982

Cell-mediated immune-type response of the American cockroach.

Cell-mediated immunity in the American cockroach, Periplaneta americana, was measured by counting nuclei in a transect through the mass of host hemocytes reacting against transplanted xenograft or autograft integument. Responses to xenografts (Leucophaea maderae) were significantly higher than control responses to autograft integument. Responses to Leucophaea xenografts increased with longer durations of xenograft exposure to hosts for up to eight days, then decreased by day sixteen. Responses to cockroach species from a phylogenetically graded series of donors were stronger and correlated with increasing phylogenetic distance between various donors and hosts (Periplaneta americana).

Animals↗
PubMed1985

Ontogeny of the invertebrate humoral immune response: studies on various developmental stages of the American cockroach (Periplaneta americana).

Earlier studies revealed that a specific adaptive humoral immune response can be induced in the American cockroach (Periplaneta americana) to the soluble protein complex, Honeybee venom (HBT). We have undertaken a series of ontogenetic studies to determine if there are differences between the protective responses of roaches representing different developmental stages. Our results indicated that the response to HBT in the immature cockroach (nymphs weighing 200-500 mg) was characterized by a significant lag period before immune protection began to develop. However, by the second week of the response, reactivity was comparable to that of the adult. Old adult male roaches (animals 5 months into adulthood) displayed a significant decline in reactivity during the early phases of the response in comparison to younger adults, and in general, appeared to be less vigorous in generating protection. Both the nymph and old adult roaches demonstrated good secondary responsiveness. The results from these experiments indicated that the developmental stage of the roach could be directly correlated to the degree of immunocompetency possessed by the animal. This is similar to the ontogenetic sequence typically found for immune reactivity in vertebrates.

Age Factors↗
PubMedDate not supplied

Transplantation immunity in the American cockroach, Periplaneta americana: the rejection of integumentary grafts from Blatta orientalis.

The results from several previous studies have indicated that the American cockroach is able to respond to integumentary xenografts, but doubt remained as to whether cockroaches could effectively discriminate between self and allogeneic differences. This was emphasized by the fact that while one study reported that Periplaneta americana responded to grafts donated by the closely related genus Blatta orientalis, the data reported elsewhere, using a different assay system, found no such reactivity. Since we have subsequently reported, using a direct histological assay, that Periplaneta can in fact recognize and destroy integumentary allografts, we initiated a study to hopefully sort out the enigma presented by previous data using Blatta as a transplant donor. Integument from Blatta orientalis was transplanted orthotopically onto Periplaneta americana, and at various time points post-transplant, scored histologically for the survival of the subcuticular epidermal layer. The results clearly demonstrated that Periplaneta reacted to the Blatta tissue, because approximately 82% of the grafts had the epidermal layer destroyed by day 7 posttransplant. The kinetics of the response to Blatta was more in line with our allograft data, which would be in agreement with other work indicating that the closer the donor and recipient are on the phylogenetic tree, the less intense the reactivity to the foreign transplant.

Animals↗
PubMed1987

Dissociation and maintenance in vitro of neurones from adult cockroach (Periplaneta americana) and housefly (Musca domestica).

A method is described for dispersal and subsequent maintenance in acute culture of neurones dissociated from ganglia of the adult cockroach (Periplaneta americana) and brain of the adult fly (Musca domestica). The effects of different media on the survival and attachment to different substrates have been investigated. About 25% of dissociated neurones survived well for periods greater than 6 days in a cockroach saline supplemented with foetal calf serum and the antibiotics fungizone and gentamicin. Fly neurones were more difficult to disperse. Fly saline supplemented with foetal calf serum and the antibiotics fungizone and gentamicin proved a successful short-term culture medium. Survival times were shorter, most neurones dying after 5 days. The demonstrated viability of such neurones has considerable potential for insecticide mode of action studies using the patch-clamp technique.

Animals↗
PubMed1994

Allatostatic neuropeptides from the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae). Identification, immunolocalization and activity.

Four allatostatic neuropeptides were isolated from extracts of the brain of the cockroach Blattella germanica. The primary structures of these peptides were assigned as Leu-Tyr-Asp-Phe-Gly-Leu-NH2 (BLAST-1), Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-2), Ala-Gly-Ser-Asp-Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-3) and Ala-Pro-Ser-Ser-Ala-Gln-Arg-Leu-Tyr-Gly-Phe-Gly-Leu-NH2 (BLAST-4). Each of the peptides showed C-terminal amino acid sequence similarity to cockroach allatostatins and blowfly callatostatins. The four peptides inhibited in vitro juvenile hormone production by corpora allata from virgin females of B. germanica. Immunoreactivity against allatostatins was seen in the lateral neurosecretory neurons and in the axonal pathway leading to the corpora allata.

Amino Acid Sequence↗
PubMed1996

Isolation of five tachykinin-related peptides from the midgut of the cockroach Leucophaea maderae: existence of N-terminally extended isoforms.

Using a radioimmunoassay (RIA) with an antiserum to the locust neuropeptide locustatachykinin I (LomTK I) and a cockroach hindgut contraction bioassay as monitors, we isolated 5 tachykinin-related peptides from an acidic extract of 600 midguts of the cockroach Leucophaea maderae. A series of 4 different reversed-phase high performance liquid chromatography (rpHPLC) column systems were required to obtain pure peptides. The sequences of the 5 isolated myostimulatory and LomTK immunoreactive peptides were determined by Edman degradation. Four of these were confirmed by mass spectrometry and chemical synthesis as: APSGFLGVRamide, NGERAPGSKKAPSGFLGTRamide, APAMGFQGVRamide and APSGFMGMRamide. The fifth peptide, APEESPKRAPSGFLGVRamide, was confirmed only by mass spectrometry. These peptides, which were designated Leucophaea tachykinin-related peptides 1-5 (LemTRP 1-5), are structurally related to tachykinin-related peptides previously isolated from a locust, blowfly and mosquito species, but showed a somewhat larger variability in their amino-acid sequence (including the carboxy terminus). The two N-terminally extended forms contain putative cleavage sites (KR and KK, respectively) and such extended tachykinins have not been previously identified in insects. All 5 LemTRPs are myotropic and induce increases in the tonus and frequency of spontaneous contractions of hindgut muscle in L. maderae. The potency of the different synthetic isoforms is very similar; they all have a stimulus threshold concentration of 2.5 x 10(-10) M and an ED50 of about 10(-9) M. The synthetic peptides were tested in RIA and found to cross react to different degrees with the antiserum to LomTK I, but it is likely that in immunocytochemistry performed earlier, all 5 forms were detected in the midgut. It is, however, not clear which isoforms are located in endocrine cells and neural fibers of the midgut, respectively.

Amino Acid Sequence↗
PubMed1989

Allometry of resting metabolic rate in cockroaches.

1. Resting VO2 at 22.7 degrees C was 0.217 +/- 0.007 ml O2/g/hr in Nauphoeta cinerea of 0.51 +/- 0.008 g body mass. 2. Whole animal resting metabolic rate for 11 cockroach species scaled allometrically: VO2 = 0.261 M0.776. 3. Allometry of resting VO2 among cockroach species is similar to that in vertebrates, and is consistent with models based on both "elastic similarity" and "biological similitude."

Animals↗
PubMed1994

Proctolin promotes vitellogenesis onset in the imaginal molt decapitated cockroach Blaberus craniifer.

The ovarian response of the imaginal molt decapitated cockroach, Blaberus craniifer, to nanomolar quantities of synthetic proctolin was evaluated. Sites binding anti-egg proteins were detected in the basal oocyte in 67% (18 out of 27) of the treated females, indicating that vitellogenin uptake is mediated by proctolin. The injected concentration of proctolin was consistent with a physiological role. The results suggest that proctolin, a putative neurotransmitter or neuromodulator, possibly acts on a non-excitable cell, the oocyte. At this level, the precise mechanism is discussed with respect to the calcium-dependent processes involved in both the proctolin myogenic effect and vitellogenin uptake in other species of cockroach.

Animals↗
PubMed1996

Free amino acids in the hemolymph of the cockroach, Blaberus discoidalis.

The concentrations of free amino acids in the hemolymph of adult males of the cockroach Blaberus discoidalis were determined by automated amino acid analysis. Eighteen of the twenty standard amino acids as well as two nonstandard amino acids (hydroxyproline and ornithine) were found. Aspartic acid and cysteine were not present. Proline levels were up to 100-fold higher than those of any other amino acid. Total amino acid concentration was comparable to those reported for most other cockroaches. The reported amino acid profile formed the basis for an organ culture medium which sustained metabolic viability of isolated B. discoidalis neuroendocrine glands and brain-gland complexes for at least 24 hr.

Amino Acids↗
PubMed1996

Isolation and characterization of eight myoinhibiting peptides from the desert locust, Schistocerca gregaria: new members of the cockroach allatostatin family.

Eight myoinhibiting peptides were purified by high performance liquid chromatography from a methanolic extract of 7000 brains of the desert locust, Schistocerca gregaria. Complete sequences were obtained via a novel, combined approach employing: (1) chemical microsequencing and (2) post-source decay analysis on a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption/ionisation. Each of the peptides shows C-terminal amino acid sequence similarity to cockroach and cricket allatostatins and to blowfly callatostatins. Therefore, these novel peptides were designated Schistocerca gregaria allatostatins (Scg-ASTs) or schistostatins and their primary structures were determined to be: Ala-Tyr-Thr-Tyr-Val-Ser-Glu-Tyr-Lys-Arg-Leu-Pro-Val-Tyr-Asn-Phe-Gly-Leu- NH2 (Scg-AST-2), Ala-Thr-Gly-Ala-Ala-Ser-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-3), Gly-Pro-Arg-Thr-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-4), Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-5), Ala-Arg-Pro-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-6), Ala-Gly-Pro-Ala-Pro-Ser-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-7), Glu-Gly-Arg-Met-Tyr-Ser-Phe-Gly-Leu-NH2 (Scg-AST-8), and Ala-Pro-Ala-Glu-His-Arg-Phe-Ser-Phe-Gly-Leu-NH2 (Scg-AST-10). Synthetic Scg-AST peptides inhibit the peristaltic movements of the oviduct of S. gregaria. Although all eight peptides show potent inhibitory effects on juvenile hormone (JH) biosynthesis by corpora allata (CA) of the cockroach Diploptera punctata, no allatostatic effects were observed on CA of the desert locust (S. gregaria).

Amino Acid Sequence↗
PubMed1991

Insect tachykinin-like peptide: distribution of leucokinin immunoreactive neurons in the cockroach and blowfly brains.

Antisera were raised against leucokinin I, a cockroach myotropic neuropeptide with some resemblance to vertebrate tachykinins. These antisera were used for immunocytochemical mapping of neurons and neurosecretory cells in the brains of a cockroach and a blowfly species. The leucokinin immunoreactive cells are distinct from neurons that can be labeled with antisera against vertebrate type tachykinins. It is suggested that leucokinin-like peptides may have roles as neurohormones and neuromodulators in the insect nervous system.

Animals↗
PubMed1984

Isolation and partial characterization of five myotropic peptides present in head extracts of the cockroach Leucophaea maderae.

Five peptides were isolated by reverse-phase HPLC from head extracts of the cockroach Leucophaea maderae. Four of the peptides were inactivated by aminopeptidase M (APM). The inability of APM to digest the fifth peptide suggests a blocked NH2-terminus. Four of the peptides were inactivated by carboxypeptidase Y (CPY). The activity of the fraction which would have contained proctolin was decreased by about 20%. The complete deactivation of proctolin by CPY indicated that a second peptide, co-eluting with proctolin but refractory to CPY digestion, was responsible for 80% of the biological activity in that fraction. Concentrations of the peptides necessary to produce a threshold response from the isolated cockroach hindgut ranged from 0.009 to 0.083 head equivalents/ml.

Aminopeptidases↗
PubMed1985

Binding of N-[propionyl-3H]propionylated alpha-bungarotoxin and L-[benzilic-4,4'-3H] quinuclidinyl benzilate to CNS extracts of the cockroach Periplaneta americana.

The nerve cord of the cockroach (Periplaneta americana) contains distinct saturable components of specific binding for the ligands N-[propionyl-3H]propionylated alpha-bungarotoxin and L-[benzilic-4,4'-3H]quinuclidinyl benzilate. N-[Propionyl-3H]propionylated alpha-bungarotoxin bound reversibly to homogenates with a Kd of 4.8 nM and Bmax of 910 fmol mg-1. The association rate constant (1.9 X 10(5) M-1 s-1) and dissociation rate constant (1.2 X 10(-4) s-1) yielded a Kd of 0.6 nM. Nicotinic ligands were found to displace toxin binding most effectively. The binding sites characterized in this way showed many similarities with the properties of the vertebrate neuronal alpha-bungarotoxin binding site. For a range of cholinergic ligands, inhibition constants calculated from toxin binding studies closely corresponded to their effectiveness in blocking the depolarizing response to acetylcholine recorded by electrophysiological methods from an identified cockroach motoneurone. The N-[propionyl-3H]propionylated alpha-bungarotoxin binding component therefore appears to be a constituent of a functional CNS acetylcholine receptor. Binding of L-[benzilic-4,4'-3H]quinuclidinyl benzilate was reversible with a Kd of 8 nM and Bmax of 138 fmol mg-1, determined from equilibrium binding experiments. The Kd calculated from the association rate constant (2.4 X 10(5) M-1 s-1) and dissociation rate constant (1.3 X 10(-4) s-1) was 1.9 nM. Muscarinic ligands were the most potent inhibitors of quinuclidinyl benzilate binding. The characteristics of this binding site resembled those of vertebrate CNS muscarinic cholinergic receptors. In contrast with vertebrate CNS, the nerve cord of Periplaneta americana contains more (approximately X 7) alpha-bungarotoxin binding sites than quinuclidinyl benzilate binding sites.

Animals↗