T-cell ratios in New York City prostitutes.
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Adult↗
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Results for “CHESS”
Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.
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Uveal melanoma presenting after cataract extraction with intraocular lens implantation.
Four patients underwent cataract extraction with implantation of intraocular lenses. Preoperative ultrasonography did not include a comprehensive diagnostic B-scan analysis. Postoperative examination revealed uveal melanomas. The clinical presentations as well as the histopathologic findings of the two enucleated eyes are discussed. These cases emphasize the necessity for B-scan ultrasonography prior to cataract surgery when media opacity prevents adequate visualization of the fundus.
Aged↗
Candida retinitis in bare lymphocyte syndrome.
Bare lymphocyte syndrome (BLS) is a rare, severe combined immunodeficiency characterized by lack of expression of HLA A, B and C antigens and the absence of B2 microglobulins. Patients with BLS exhibit functional deficiency of both T and B cells resulting in bacterial as well as viral and fungal infection. Ophthalmic findings in this group of disorders have not been reported. We present a case of candida retinitis in a terminally ill 5-year-old girl with BLS.
Amphotericin B↗
A novel 26 kilodalton antigen expressed on the surface membrane of activated T cells.
We have identified and characterized the tissue distribution of the antigen recognized by a novel monoclonal antibody (mAb) 1B10, raised against an activated gammadelta T cell clone. Immunohistochemistry of tissue sections, and analysis of single cell suspensions by flow cytometry revealed that mAb 1B10 weakly reacted with <6% of normal human peripheral blood mononuclear cells (PBMC). After 5-6 days of in vitro culture of PBMC activated with phytohemagglutinin (PHA), 55% of the CD4+ and 25% of the CD8+ T cells became 1B10+. 1B10 expression was maintained on long term cultured interleukin 2 (IL-2)-dependent T cell receptor (TCR) alphabeta+ and gammadelta+ clones, and importantly, in contrast to resting T cells, the majority of in vivo activated synovial T lymphocytes from a patient with rheumatoid arthritis were 1B10+. In addition, myelo-monocytic U927 cells, tissue macrophages and some epithelia and fibroblasts were found to react with mAb 1B10. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of molecules immuno-precipitated by mAb 1B10 from radio-iodinated cell surface membrane lysates of T lymphocyte and U937 cells revealed 26 and 29 kiloDalton (kDa) glycoproteins respectively. In conclusion, mAb 1B10 recognizes a novel < > appearing 26 kDa T cell activation antigen that may be useful for further studies of activated T cells in health and disease.
Antibodies, Monoclonal↗
Fine-needle aspiration biopsy diagnosis of follicular variant of papillary thyroid cancer: therapeutic implications.
OBJECTIVE: Frozen-section analysis of follicular lesions is often inconclusive because capsular or vascular invasions are the hallmarks of malignancy with this technique and may not be seen in the particular field studied. Using preoperative fine-needle aspiration biopsy specimens, we attempted to identify malignant follicular lesions and compare these results against frozen-section analysis. METHODS: A retrospective study of 1000 consecutive thyroid fine-needle aspiration biopsy specimens was performed. Surgical pathologic correlation was available in 179 cases. RESULTS: Fine-needle aspiration biopsy yielded a suspicious or positive rate of 23%. Surgical pathology was available in 179 patients, of which 95% had thyroid cancer. The follicular variant of papillary cancer was identified in 26 cases, or 15% of the positive cases. Frozen-section analysis yielded false-negative results in 7 of these 26 cases (27%). CONCLUSIONS: Preoperative recognition of the follicular variant of papillary cancer by fine-needle aspiration biopsy may reduce the overall incidence of false-negative frozen-section findings.
Biopsy, Needle↗
A biomechanical analysis of intrascaphoid compression using the Herbert scaphoid screw system. An in vitro cadaveric study.
The purpose of this study was to evaluate the compressive capabilities of the Herbert scaphoid screw system. A transverse osteotomy was performed at the waist of eight scaphoids removing a 3 mm wafer of bone. A custom-designed load washer was inserted within this defect to measure intrascaphoid compression during Huene guide application, during screw insertion, after screw insertion and after guide release. The intrascaphoid compression was noted to peak during screw insertion; however it dramatically decreased without any clinical subjective evidence of decreased insertional torque by the surgeon. The average final compression as a percentage of maximal compression was 38 (SD 26) %.
Aged↗
Load relaxation and forces with activity in Hoffman external fixators: a clinical study in patients with Colles' fractures.
A small-frame Hoffman external fixation bar instrumented with strain gauges to quantify bending and torsional forces was applied to 4 patients with a displaced metaphyseal fracture of the distal radius. Measurements were taken during surgery as well as at 1, 3, and 6 weeks after surgery during activities of daily living and hand therapy mobilization. Radiographs also were taken before and after reduction and at each subsequent visit. Force decay occurred after reduction of the fracture, averaging only 26% of the initial distraction forces by 5 minutes. These forces plateaued and did not significantly change over the subsequent 40-minute observation period. There was no correlation between carpal height index and the forces measured in the external fixator. Significant changes in external fixator forces were measured during activities of daily living and hand therapy mobilization, but these returned to baseline after the activities were performed. The most provocative activities studied were twisting a doorknob and lifting heavy objects. These activities should be performed with caution by patients with unstable distal radial fractures.
Activities of Daily Living↗
The role of T4 in immune function and dysfunction.
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Acquired Immunodeficiency Syndrome↗
The effect of cement restrictors on the occlusion of the humeral canal: an in vitro comparative study of 2 devices.
An in vitro study investigated the efficacy of cement restrictor devices on cement containment and penetration within the humerus. Eight pairs of preserved humeri were prepared using advanced cementing technique followed by insertion of 1 of 2 cement restrictors. Low-viscosity cement was injected followed by sham humeral stem insertion. Each specimen was sectioned into transverse 10-mm slices. Slices were photographed and digitized to quantify the cement/stem and canal cross-sectional area. Cement penetration was determined from the ratio of cement area to canal area. A significant increase in cement penetration was observed among slices from distal to proximal for both restrictors (P = .02). There was no significant difference in cement penetration, leakage, or migration between restrictors. Five of the 8 specimens migrated, with means of 21.5 +/- 25.0 mm and 24.0 +/- 36.0 mm for the polyethylene and silicone restrictors. Leakage or migration resulted in a significant decrease in cement penetration with the polyethylene restrictors (P = .001). In the silicone restrictor group, migration resulted in decreased cement penetration (P = .04). When using advanced cementing techniques, intramedullary restrictors allow improved cement penetration; however, they do not ensure cement containment.
Arthroplasty, Replacement↗
Patellar position after total knee arthroplasty: influence of femoral component malposition.
Patellar shift, tilt, and rotation were analyzed in 7 cadaveric knee specimens during simulated quadriceps loading, in the intact knee, and after implant reconstruction. Femoral component medialization, lateralization, and external rotation were also investigated. Relative motion of the patella with respect to the femur was measured using an electromagnetic tracking system. The spatial position of the patella did not change with standardized total knee arthroplasty (P <.05). After malpositioning of the femoral component, patellar rotation also did not change (P >.05); however, patellar tilt was altered by femoral component external rotation malposition (P <.05), and patellar shift was affected by all femoral component malpositions (P <.05). The spatial position of the patella relative to the femoral shaft was changed with any femoral component malposition, suggesting that the soft tissues were abnormally tensioned. This could result in subsequent wear on the patellar component and, therefore, early failure.
Arthroplasty, Replacement, Knee↗
Late disassembly of a modular acetabular component. A case report.
Disassembly of metal-backed acetabular components used for total hip arthroplasty is becoming more common in both preassembled and modular designs. This complication is usually associated with a dislocation of the femoral component or other radiographic evidence of dissociation. A case of late disassembly of the acetabular component without dislocation or radiographic evidence is presented. The authors believe this complication should be considered, along with infection and aseptic loosening, in all cases of late development of pain following total hip arthroplasty.
Adult↗
Effect of the posterior cruciate ligament in knee-joint proprioception in total knee arthroplasty.
The primary purpose of the study was to examine the role of the posterior cruciate ligament (PCL) in knee-joint proprioception after total knee arthroplasty (TKA). Knee-joint proprioception was measured in 10 patients with nonsacrificed PCL TKAs and 10 with sacrificed PCL TKAs. Knee-joint proprioception was evaluated through reproduction of static knee angles using a Penny and Giles electrogoniometer. The primary variable was absolute angular error (AAE). AAE was defined as the absolute value of the difference between the test angle and the patient's perceived version of the test angle. Proprioception deficit was compared to the WOMAC questionnaire which evaluates pain, stiffness, and physical function of the lower extremity. No significant difference was found between the nonsacrificed PCL TKA (4.33 degrees +/- 1.52 degrees) and sacrificed PCL TKA (4.38 degrees +/- 1.39 degrees) AAE values (P > .4). Furthermore, no significant differences were observed in the WOMAC questionnaire scores for all three parameters between the two types of knee prosthesis (P > .35). The current findings suggest that the preservation of the PCL in TKA may not improve knee-joint proprioception and subsequently may not improve TKA functional performance.
Aged↗
The understanding of contact-dependent T-cell helper function in molecular, cellular and physiological detail.
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Animals↗
The specification of olfactory neurons.
Recent studies have shed light on the different relationships between odorant receptor expression and the specification of neural identity in the olfactory systems of vertebrates and invertebrates. In mice, neuronal identity and axon guidance are specified by the single expressed olfactory receptor, whereas in C. elegans, neuronal identity appears to be independent of receptor expression.
Animals↗
Odorant receptors: axon contact-mediated diversity.
To make the most of a small number of neurons, the nematode olfactory system includes neurons that are bilaterally symmetrical in morphology, but differ in the sets of genes they express. An intriguing recent example is the axon contact-mediated asymmetry in expression of the str-2 odorant receptor gene.
Animals↗
Olfactory neurons are interdependent in maintaining axonal projections.
In mice, individual olfactory neurons express one of the thousand distinct olfactory receptor genes [1] [2] [3]. Neurons that express a given receptor converge on distinct loci in the olfactory bulb to form structures called glomeruli [4] [5] [6]. The olfactory receptor is involved in an instructive manner in this axonal convergence [6] [7] but little is known about the mechanisms involved in maintaining convergence. We have previously created a transgenic olfactory receptor locus that functions independently of the endogenous loci [8]. Here, we show that, although the projections of neurons expressing this ectopic transgenic olfactory receptor always converge in newborn mice, surprisingly, in adult mice, convergence is not always maintained. Moreover, in adult mice there is a positive correlation between the number of neurons expressing the transgenic receptor and the probability of maintaining convergence. These observations, taken together with the variability observed in wild-type [4] [6] and genetically manipulated mice ([6] and our unpublished observations), suggest that olfactory neurons require the presence of other similar axons to maintain a glomerulus. We call this phenomenon interdependence.
Animals↗
Allele-specific expression patterns of interleukin-2 and Pax-5 revealed by a sensitive single-cell RT-PCR analysis.
Autosomal genes that are subject to random allelic inactivation (RAI), like imprinted genes [1] and genes subject to X-inactivation [2], require mechanisms that dictate the differential transcriptional regulation of two sequence-identical alleles. RAI genes include olfactory receptor genes [3], and the various genes encoding antigen receptors on lymphocytes (immunoglobulin genes, T cell receptor genes and NK receptor genes [4] [5] [6] [7]). These observations raise the possibility that other genes might be similarly regulated. Moreover, an interesting possibility is that certain genes might be monoallelically expressed in some cells and biallelically expressed in others. Recently, reports of monoallelic expression of interleukin-2 (IL-2) [8] [9] and IL-4 [10] [11] have raised the possibility that the cytokine gene family may be subject to monoallelic expression. Another report suggests that the gene encoding the transcription factor Pax-5, which is involved in B-cell (and cerebellar) development [12] [13], is also subject to monoallelic expression [14]. Using a novel single-cell reverse transcription-polymerase chain reaction (RT-PCR) approach, we have analyzed the IL-2 and Pax-5 genes in mice. We found that IL-2 is monoallelically transcribed in some T cells and biallelically transcribed in others, raising interesting questions regarding cytokine gene regulation. Additionally, our analyses suggest that Pax-5 is consistently biallelically transcribed. Thus, the IL-2 gene and other cytokine genes may be regulated in a stochastic manner that results in 0, 1 or 2 alleles of a given cytokine gene expressed in each T cell. This type of regulation could account for the wide variety of different combinations of cytokine genes expressed in individual T cells and therefore plays a role in the generation of T cells with a range of different effector functions.
Alleles↗
Olfactory axons: a remarkable convergence.
Thousands of neurons expressing a given mouse odorant receptor project to a few glomeruli in the olfactory bulb. New observations on mice expressing small odorant receptor transgenes provide support for the idea that interdependence is involved in the maturation of this remarkable convergence.
Animals↗