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Epidemiology of Candida infection. II. Application of biochemical methods for typing of Candida albicans strains.

Biochemical profiles of 350 C. albicans isolates from five towns in Poland and from Freiburg in Germany were determined on the basis of nine biochemical tests of Odds and Abbott method. API 20 C AUX system and additionally a resistogram. The analysis of the strains according to Odds' and Abbotts's system showed that investigated strains can be typed into 9 profile codes of common biochemical patterns. There were some differences among the profiles according to their geographical origin and anatomical sources of the isolation. On the basis of the ability C. albicans strains to assimilate of carbon sources, 350 isolates were categorised into 13 separate auxotrophic profiles with the major one: 2,576,174 accounting for 81% of the total. The majority of the investigated isolates were susceptible to antifungal agents (83%). A disproportionate distribution of auxotrophic profiles limited the use of resistogram method and API 20 C AUX as systems for typing C. albicans strains. On the other hand, the method of Odds and Abbott provides valuable criteria for typing of C. albicans.

Antifungal Agents↗

Epidemiology of Candida infection. III. A proposal for a new typing system for Candida albicans strains.

A proposal of a new typing system for C. albicans strains in this paper is presented. Our system was elaborated on the basis of computer analysis of the results received using various already existing methods (killer system, resistogram, API system and Odd's and Abbott's method) for typing of 350 C. albicans strains. The strains originated from various anatomical and geographical sources. For the new system we have chosen three characters: sensitivity to two killer strains (Pichia sp. STUMM 1035, Hansenula sp. STUMM 1034) and urea assimilation test. The computer-aided program, used as an analytical tool, permitted the selection of 8 types among the strains. The majority of tested isolates belonged to types 6 and 7. We observed differences in the distribution of types according to the geographical and anatomical origin of the strains. The criteria adopted in our system can be useful in typing of C. albicans for epidemiological studies.

Bacterial Typing Techniques↗