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At least 469 records · Page 26Linked to original sources
PubMed1985

Topographic organization of interaural intensity difference sensitivity in deep layers of cat superior colliculus: implications for auditory spatial representation.

Sensitivity to interaural intensity difference (IID) was examined for 103 neurons in the deep layers of superior colliculus (SC) in ketamine barbiturate-anesthetized cats. Noise stimuli were presented dichotically, and IID sensitivity functions were generated while keeping the average binaural intensity (ABI) of stimulation constant. Neurons of three binaural classes were found to be IID sensitive. Neurons receiving excitatory contralateral input and inhibitory ipsilateral input (EO/I cells, 55% of sample) had steplike IID functions, with maximum response at IIDs corresponding to contralateral azimuths (positive IIDs), total suppression at IIDs corresponding to ipsilateral azimuths (negative IIDs), and cutoffs at different positions along the IID axis for different neurons. Neurons responsive only to binaural stimulation (OO/F cells, 15% of sample) had IID functions with a sharp peak in the range of 0 to 10 dB IID. Cells receiving excitatory input contralaterally and a facilitatory ipsilateral input (EO/F cells, 7% of sample) had IID functions of intermediate shape, with a peak in the range of 10 to 20 dB IID and a sharper cutoff near zero IID than at larger positive IIDs. The sharpness of IID cutoff for EO/I cells was quantified by measuring an 80% IID dynamic range. Neurons with 80% IID dynamic ranges of less than 26 dB were judged to have sharp cutoffs. The position along the IID axis of the IID cutoff for these cells was quantified by recording the IID at which the response was at 50% of maximum (half-maximal IID). A topographic organization of EO/I cells with sharp IID cutoffs was found along the rostrocaudal axis of SC, such that rostral EO/I cells had IID functions with half-maximal IIDs near zero, while increasingly caudal EO/I cells had progressively larger (positive) half-maximal IIDs. Although detailed maps could not be obtained in individual animals, the topography was observed in each of nine experiments in which EO/I cells were located in two or more rostrocaudal locations (P = 0.00002). The effect of stimulus level on the stability of IID cutoff was examined for 13 EO/I cells. The majority (85%) showed less than 10 dB variation in half-maximal IID across a range of suprathreshold ABIs, indicating that EO/I cells in SC generally exhibit stability in cutoff with changes in intensity of broadband stimuli.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed2006

Combining low and high mass ion accumulation for enhancing shotgun proteome analysis by accurate mass measurement.

A simple procedure is described that increases sensitivity and dynamic range for the analysis of a proteome batch digest by FT-ICR mass spectrometry. Ions at the low and high mass ranges are preferentially collected using two different sets of tuning conditions. By combing data collected using tuning conditions that favor low mass (m/z < 2000) and high mass (m/z > 2000) ions, 277 proteins are identified for a whole cell lysate of Methanococcus maripaludis in a single HPLC-MALDI FT-ICR mass spectrometry experiment, a 70% improvement compared with previous analyses using a wide mass range acquisition. This procedure improves the detection of low abundance ions and thereby increases the range of proteins that are observed. Because the observed mass range is effectively narrower for each spectrum, mass calibration is more accurate than for the standard method that provides a wide range of masses. The trap plate potential on the analyzer cell may be set to a higher value than used for wide mass range measurements, increasing the ion capacity of the analyzer cell and extending the dynamic range, while still maintaining mass accuracy.

Bacterial Proteins↗
PubMed2000

Adaptive rescaling maximizes information transmission.

Adaptation is a widespread phenomenon in nervous systems, providing flexibility to function under varying external conditions. Here, we relate an adaptive property of a sensory system directly to its function as a carrier of information about input signals. We show that the input/output relation of a sensory system in a dynamic environment changes with the statistical properties of the environment. Specifically, when the dynamic range of inputs changes, the input/output relation rescales so as to match the dynamic range of responses to that of the inputs. We give direct evidence that the scaling of the input/output relation is set to maximize information transmission for each distribution of signals. This adaptive behavior should be particularly useful in dealing with the intermittent statistics of natural signals.

Adaptation, Physiological↗
PubMed2003

Quantitative detection of protein arrays.

We introduce a quantitative method that utilizes scanning electron microscopy for the analysis of protein chips (SEMPC). SEMPC is based upon counting target-coated gold particles interacting specifically with ligands or proteins arrayed on a derivative microscope glass slide by utilizing backscattering electron detection. As model systems, we quantified the interactions of biotin and streptavidin and of an antibody with its cognate hapten. Our method gives quantitative molecule-counting capabilities with an excellent signal-to-noise ratio and demonstrates a broad dynamic range while retaining easy sample preparation and realistic automation capability. Increased sensitivity and dynamic range are achieved in comparison to currently used array detection methods such as fluorescence, with no signal bleaching, affording high reproducibility and compatibility with miniaturization. Thus, our approach facilitates the determination of the absolute number of molecules bound to the chip rather than their relative amounts, as well as the use of smaller samples.

Antibodies↗
PubMed2000

3D imaging: wave front sensing utilizing a birefringent crystal

Utilizing a rotatable thin birefringent crystal and two polarizers, the three-dimensional (3D) wave front of any light wave can be measured conveniently. In first experiments phase-front distortions as small as 15 &mgr;m are detected with a dynamic range of 3 mm and a spatial resolution of 50 &mgr;m. Such a dynamic range and spatial resolution exceed the performance of conventional wave front sensors of, e.g., the Shack-Hartmann type, significantly. Furthermore, the new system is rather insensitive against mechanical instabilities in opposition to interferometric and holographic techniques.

Journal Article↗
PubMed2006

From ultraslow to fast lithium diffusion in the 2D ion conductor Li0.7TiS2 probed directly by stimulated-echo NMR and nuclear magnetic relaxation.

7Li stimulated-echo NMR and classical relaxation NMR techniques are jointly used for the first time for a comprehensive investigation of Li diffusion in layer-structured Li0.7TiS2. One single 2D Li diffusion process was probed over a dynamic range of almost 10 orders of magnitude. So far, this is the largest dynamic range being measured by 7Li NMR spectroscopy directly, i.e., without the help of a specific theoretical model. The jump rates obey a strict Arrhenius law, determined by an activation energy of 0.41(1) eV and a preexponential factor of 6.3(1)x10(12) s-1, and range between 1x10(-1) s-1 and 7.8x10(8) s-1 (148-510 K). Ultraslow Li jumps in the kHz to sub-Hz range were measured directly by recording 7Li spin-alignment correlation functions. The temperature and, in particular, the frequency dependence of the relaxation rates fully agree with results expected for 2D diffusion.

Journal Article↗
PubMed2006

Fully automated quantitation of hepatitis B virus (HBV) DNA in human plasma by the COBAS AmpliPrep/COBAS TaqMan system.

BACKGROUND: The measurement of HBV DNA levels has become the most direct and reliable method used for accurate diagnosis and prognosis of acute and chronic HBV infection []. Nucleic acid amplification testing (NAT detection) also reduces the pre-seroconversion window period. The method can be used to aid in the management of HBV infection, by the identification of individuals with high levels of viral replication who might benefit from antiviral therapy, monitoring patients on therapy, and identification of the development of resistance. OBJECTIVES: Evaluation of the novel COBAS AmpliPrep/COBAS TaqMan HBV Test, which combines automated extraction of DNA on the COBAS AmpliPrep Instrument (CAP), coupled with real-time PCR on the COBAS TaqMan Analyzer (CTM), thus greatly reducing hands-on time during sample preparation and amplification/detection. The assay fulfils the current requirements: a highly sensitive HBV DNA detection reagent which is calibrated to the International WHO Standard to reliably quantify HBV genotypes A-G in plasma with a very broad measuring range, thereby minimizing laborious repeat testing. STUDY DESIGN: The test was evaluated for sensitivity, dynamic range, precision, clinical and analytical specificity, genotype inclusivity, interfering substances, and correlation with other tests for the detection of HBV DNA (COBAS Amplicor HBV Monitor Test, COBAS TaqMan HBV Test for Use with the High Pure System and VERSANT HBV DNA 3.0 Assay). RESULTS AND CONCLUSION: A fully automated system for sample preparation, amplification and quantitation of HBV DNA was developed that demonstrates a nearly 7-log dynamic range up to 1.1E+08IU/mL, an assay sensitivity (95% hit-rate) of 4-12IU/mL and an equivalent detection of genotypes A-G plus a prevalent pre-core mutant. The results obtained with the new test show a good correlation to titers obtained with other platforms.

Automation↗
PubMed1976

Physiology of peripheral neurons innervating otolith organs of the squirrel monkey. II. Directional selectivity and force-response relations.

1. The directional selectivity of peripheral otolith neurons was studied in the barbiturate-anesthetized squirrel monkey (Saimiri sciureus). Each unit's most sensitive axis was characterized by a functional polarization vector. The direction of a centrifugal force was varied with respect to the vector and to the macular plane. The neurons respond in an excitatory manner to shearing forces orthogonally disposed to the vector. The sensitivity to orthogonal shears was usually 10-15% of the sensitivity to parallel shearing forces. There was no significant response to orthogonal compressions, nor did compressions modify the response to shearing forces. 2. Force-response functions were obtained in the stimulus range of +/- 4.92 g. Forces were directed parallel to each unit's polarization vector. The functions are sigmoid shaped and possess both inhibitory and excitatory plateaus. The presumed physiological range of +/-1 g is represented in the lower (concave upward) portion of the function and has a dynamic range, expressed in terms of response magnitude, amounting to 20-40% of the potential dynamic range of the neuron. 3. There was considerable variation among units in their +/- 4.92 g force-response curves. The salient features of the functions are described by three factors, tentatively identified as a transduction gain, a receptor bias, and a mechanical gain. Both the resting discharge (do) and the +/-1 g sensitivity (so) vary in the same direction with changes in the factors related to transduction gain and receptor bias. It is shown that this covariation provides a quantitatively precise explanation for the positive relation between do and so.

Animals↗
PubMed1994

A new approach to optical imaging applied to rat barrel cortex.

Several groups have described using intrinsic optical imaging to form images of activity patterns in the cortex. Because the signal is small, the general approach has been to use expensive camera equipment with a high dynamic range to make these measurements. However, by using signal averaging to compensate for lower dynamic range, images can be obtained using equipment already available in many laboratories. This modified technique has been used for imaging activity in 'barrel' cortex of the rat. A map of the representation of a single whisker as determined by the imaging technique corresponded well with a similar map made using standard electrophysiology. A map of several whiskers was made by overlaying images of single-whisker representations. The details of the images differ from those previously described. Possible mechanisms for the signal are discussed.

Animals↗
PubMed1997

Response properties of neurons in the inferior colliculus of the monaurally deafened ferret to acoustic stimulation of the intact ear.

Response properties of neurons in the central nucleus of the inferior colliculus (ICC) were investigated after unilateral cochlear removal at various ages during infancy. Nineteen ferrets had the right cochlea surgically ablated, either in adulthood or on postnatal day (P) 5, 25, or 40, 3-18 mo before recording. Adult ablations were made on the same day as ("acute," n = 3), or 2-3 mo before ("chronic," n = 3), recording. Two ferrets were left binaurally intact. Single-unit (n = 702) and multiunit (n = 1,819) recordings were made in the ICC of barbiturate-anesthetized ferrets ipsilateral (all ages) or contralateral (P5 and acute adult only) to the intact ear. In binaurally intact animals, tonal stimulation of the contralateral ear evoked excitatory activity at the majority (94%) of recording loci, whereas stimulation of the ipsilateral ear evoked activity at only 33% of recording loci. In acutely ablated animals, the majority of contralateral (90%) and ipsilateral (70%) loci were excited by tonal stimulation of the intact ear. In chronically ablated animals, 80-90% of loci were excited by ipsilateral stimulation. Single-unit thresholds were generally higher for low-best frequency (BF) than for high-BF units, and higher in the ipsilateral than in the contralateral ICC. Analysis of covariance showed highly significant differences between all of the ipsilateral and contralateral groups, but no effects of age at ablation or survival time following ablation, other than that the group ablated at P25 had higher mean ipsilateral thresholds than the groups ablated at P5 or, acutely, in adulthood. Cochlear ablation at P5, 25, or 40 resulted in a significant increase in dynamic ranges of ipsilateral ICC unit rate-intensity functions relative to acutely ablated animals. Dynamic ranges of units in the contralateral ICC of P5-ablated ferrets were also significantly increased compared with those of acutely ablated animals. Cochlear ablation at P5, 25, or 40 resulted in a significant increase in single-unit spontaneous discharge rates in the ICC ipsilateral but not contralateral (P5 only) to the intact ear. These data show that unilateral cochlear removal in adult ferrets leads to a rapid and dramatic increase in the proportion of neurons in the ICC ipsilateral to the intact ear that is excited by acoustic stimulation of that ear. In addition, the data confirm that, in ferrets, cochlear removal in infancy leads to a further increase in responsiveness of individual neurons in the ipsilateral ICC. Finally, the data show that responses in the ICC contralateral to the intact ear are largely but not completely unchanged by unilateral cochlear removal.

Acoustic Stimulation↗
PubMed2002

Significance of talar distortion for ankle mobility in idiopathic clubfoot.

The abnormal bony feature found most consistently in clubfeet is talar distortion. The significance of the talar distortion for mobility of the tibiotalar joint was investigated. Twenty-seven congenital clubfeet in 19 patients were examined at a minimal followup of 20 years. In all patients Turco's posteromedial release was done because of idiopathic clubfoot. Radiographic assessment of the feet included measurement of the talocalcaneal angle and index, and the tibiocalcaneal angle. The degree of talar flattening was estimated by the ratio of the curvature of the talar dome to the length of the talar bone (radius to length ratio). Three-dimensional gait analysis was done to assess the dynamic range of ankle motion. The static range of motion was measured with a goniometer. The degree of talar flattening correlated significantly with the dynamic range of ankle motion but not with the static mobility. For assessment of idiopathic clubfoot, evaluation of talar flattening should be done because of its significance for dynamic ankle mobility.

Adult↗
PubMed2000

Suprathreshold stochastic resonance in multilevel threshold systems

A new form of stochastic resonance (SR) that occurs in multilevel threshold signal detectors is reported. In contrast to classical SR, which extends the dynamic range of threshold detectors to subthreshold signal levels, this new form of SR extends the dynamic range to suprathreshold signal strengths. The effect is most dominant, and can outperform networks based on standard engineering design, when all thresholds adapt to the dc level of the signal. This has an interesting analogy to dc adaptation in neurons. The possible connection between these two effects is discussed.

Journal Article↗
PubMed1989

Forward masking patterns produced by intracochlear electrical stimulation of one and two electrode pairs in the human cochlea.

Three psychophysical forward masking studies were conducted on a multichannel cochlear implant patient. The first study investigated the masking pattern produced by a bipolar electrode pair at different stimulus currents. It was found that the masking pattern for a single-masker bipolar electrode pair had a maximum located at an electrode position where the masker and probe coincided. The spread of the masking pattern was not symmetrical about the maximum. The amount of masking decreased very rapidly toward the apical direction and less rapidly toward the basal direction from the position of the maximum. As the stimulus current increased, the amount of masking at the maximum increased and the masking pattern broadened toward the base. The second study investigated the masking pattern produced by the activation of single bipolar electrode pairs with different spatial extents. The spatial extent of a bipolar electrode pair is defined as the distance between the apical and basal electrode members of the bipolar pair. With a small spatial extent (1.5 mm), the more basal electrode pairs (higher threshold and smaller dynamic range) produced broader masking patterns than the more apical electrode pairs (lower threshold and wider dynamic range), suggesting that there was more current spread at the basal region. With a larger spatial extent (4.5 mm), an additional secondary masking maximum was observed in the vicinity of the apical electrode member of the masker; this was observed only when the apical electrode member lay within the low-threshold apical region. The third study investigated the masking patterns produced by two loudness balanced bipolar masker electrode pairs activated within a stimulus period (inverse of the pulse repetition rate). The biphasic current pulses delivered to the two electrode pairs were nonoverlapping in time. It was found that, at any probe electrode position, the amount of masking produced by the two combined bipolar electrode pairs approximately followed the greater of the two maskings produced respectively by the two individual bipolar masker electrode pairs.

Cochlea↗
PubMed2006

[Vocal efficiency parameters in children and adolescents: effect of physical development and singing activity].

The influence of physical development and singing activity on vocal efficiency in children and adolescents is well known from clinical experience. There is, however, no comparative study between singing and non-singing children which also considers the influence of age and gender. Therefore, standard values for evaluation in clinical practice are missing. We examined the following parameters in 164 healthy children and adolescents (90 boys, 74 girls; 11-16 years), 86 without singing activity (group A) and 78 members of children's and youth choirs (group B): frequency and dynamic range of voice and its borders with a voice range profile, mean fundamental frequency (normal and loud phonation), maximum voice intensity, and maximum duration of intonation. The statistical analysis was performed using three-way ANOVA. We found significantly higher ranges of frequency and intensity in singing children and adolescents (p<0.0001). The borders of the dynamic range and the upper border of the frequency range were significantly higher in group B (p<0.0001). The boys in group B used a higher mean fundamental frequency during loud phonation. There were no significant differences between groups in maximum voice intensity (p=0.051) but a tendency towards higher values in singing children. As an unexpected result, we found significantly higher values in maximum duration of intonation in group A (p<0.0001) independent of age and gender, which seems to be related to the methods used. Regular training of the singing voice results in positive effects on several voice parameters in children as well as adolescents. Our results can be used for estimating standard values in professional clinical and educational care of young singing voices and non-singing children. For this purpose, voice range profile is particularly suitable.

Adolescent↗
PubMed1995

Responses of neurons in ventrolateral orbital cortex to noxious visceral stimulation in the rat.

In pentobarbital-anesthetized rats, responses of single neurons in ventrolateral orbital cortex (VLO) to noxious visceral (colorectal distension, CRD) and cutaneous stimulation were recorded. Of 71 neurons identified on the basis of spontaneous activity, 44 responded to CRD. CRD caused inhibition of neuronal activity in 38, facilitation of activity in four and 'mixed' responses in two of these cells. Cutaneous receptive fields were identified in 31 CRD-responsive and 10 CRD-non-responsive neurons. Cutaneous receptive fields were large and bilateral. 25 CRD-responsive cells responded only to noxious cutaneous stimulation, six had wide dynamic range responses. Six CRD-non-responsive cells responded only to noxious stimuli, four had wide dynamic range responses. No VLO neuron responded only to innocuous stimuli. These data are consistent with involvement of VLO in visceral nociception, possibly in non-discriminative aspects of nociception.

Animals↗
PubMed2005

Simultaneous determination of pH, urea, acetylcholine and heavy metals using array-based enzymatic optical biosensor.

An array-based optical biosensor for the simultaneous analysis of multiple samples in the presence of unrelated multi-analytes was fabricated. Urease and acetylcholinesterase (AChE) were used as model enzymes and were co-entrapped with the sensing probe, FITC-dextran, in the sol-gel matrix to measure pH, urea, acetylcholine (ACh) and heavy metals (enzyme inhibitors). Environmental and biological samples spiked with metal ions were also used to evaluate the application of the array biosensor to real samples. The biosensor exhibited high specificity in identifying multiple analytes. No obvious cross-interference was observed when a 50-spot array biosensor was used for simultaneous analysis of multiple samples in the presence of multiple analytes. The sensing system can determine pH over a dynamic range from 4 to 8.5. The limits of detection (LODs) of 2.5-50 microM with a dynamic range of 2-3 orders of magnitude for urea and ACh measurements were obtained. Moreover, the urease-encapsulated array biosensor was used to detect heavy metals. The analytical ranges of Cd(II), Cu(II), and Hg(II) were between 10 nM and 100 mM. When real samples were spiked with heavy metals, the array biosensor also exhibited potential effectiveness in screening enzyme inhibitors.

Acetylcholine↗
PubMed1999

Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing.

Chemiluminescence detection was combined with capillary isoelectric focusing to perform protein analysis with high sensitivity. Luminol-H2O2 chemiluminescence was utilized, and heme proteins such as cytochrome c, myoglobin and peroxidase were analyzed. The proteins were focused by use of Pharmalyte 3-10 as ampholytes. Hydroxypropylmethyl-cellulose was added to the sample solution in order to easily reduce protein interactions with the capillary wall as well as the electroendoosmotic flow. The focused proteins were transported by salt mobilization to chemiluminescence detection cell equipped with an optical fiber. The present method showed significantly high sensitivity and wide dynamic range; the detection limit for cytochrome c was 6 x 10(-9) M and the linear dynamic range was greater than two-orders of magnitude (up to 2 x 10(-6) M).

Calibration↗
PubMed1997

Input from the medial nucleus of trapezoid body to an interaural level detector.

The medial nucleus of the trapezoid body (MNTB) contains components of a neural network that functions as an interaural level difference (ILD) detector. In the cat, lateral superior olivary (LSO) neurons compare the contralateral inhibitory input from the MNTB with an excitatory input form the ipsilateral anteroventral cochlear nucleus to extract information about binaural stimuli. To better specify the inhibitory inputs to the LSO and gain a better understanding of the inhibitory component of the LSO network, the response characteristics of MNTB neurons were examined in cats under stimulus conditions similar to those used to study LSO inhibitory responses. The inhibitory tuning curves of LSO units were wider than the tuning curves of MNTB units. Hence, MNTB neurons with similar, but not identical, characteristic frequencies converge to provide inhibitory input to single LSO neurons. Variations in the number of converging MNTB inputs produced a range of LSO excitatory-inhibitory threshold differences, thus creating a coding mechanism for representing the ILD. Convergence of MNTB inputs also increased the dynamic range over which contralateral stimulus level effects LSO binaural responses beyond the dynamic ranges of individual MNTB units, thus expanding the ILD range encoded by the LSO network. The differences between the first-spike latencies of MNTB and LSO tone burst responses were small and the precision of the LSO first-spike discharges was significantly greater than that of MNTB units. As tone bursts delivered simultaneously to the two ears can consistently inhibit LSO first-spike discharges, the inhibitory input must match the LSO precision by converging a number of the more variably timed MNTB discharges. Because of their precision LSO first-spike discharges may be used to encode interaural time-of-arrival differences of mid- to high-frequency transients. These findings add to the foundation for a comprehensive network model that describes the inputs to the LSO as point processes, delimits the biophysical mechanisms underlying excitatory and inhibitory interactions at the single neuron level, and reveals how these inputs determine the response to different binaural stimulus conditions.

Acoustic Stimulation↗