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[Total bacterial count and the occurrence of Streptococcus mutans and Streptococcus sanguis in the air of various work areas].

In the various rooms of the Würzburg Dental School, the total germ contamination of the air and the incidence of S. mutans and S. sanguis strains was studied. The total number of organisms found as well as the number of the two polysaccharide-producing organisms varied considerably. The presence of the two streptococcal strains is not restricted to the air in dental surgeries. It is considered to be an additional risk that by far the highest incidence of the cariogenic S. mutans strain is found in the air of the rooms of the outpatient clinic for restorative dentistry and periodontology.

Air Microbiology↗

[The morphology of microcolonies as a criterion for recognizing bacteria in the differentiation of Streptococcus pyogenes and Streptococcus group B].

The authors studied the diagnostic importance of the morphology of microcolonies of S. pyogenes and group B streptococci in comparison with the currently used tests for the differentiation of these two species: the bacitracin test, the hydrolysis of sodium hippurate and the CAMP test. The standard tests proved to be positive in 94-97% and microcolonies had typical morphology in 86-95.7%. The statistical indices of the diagnostic effectiveness of differential tests varied within 93.8-98.9%. The diagnostic value of the study of the morphology of colonies was characterized by the following data: the sensitivity and prognostic negative value of the study were 95% for S. pyogenes and 86-89% for group B streptococci, while its specificity and prognostic positive value were 100% due to the absence of false positive results.

Bacteriological Techniques↗

A survey of Streptococcus pneumoniae, Streptococcus zooepidemicus, Salmonella spp., Bordetella bronchiseptica and Sendai virus in guinea pig colonies in Japan.

S. pneumoniae, S. zooepidemicus, Salmonella spp., B. bronchiseptica and Sendai virus were examined in a total of 45 guinea pig colonies (17 institutional and 28 breeder's colonies) of Hartley strain, and found to be positive in 6, 3, 5, 20 and 14 colonies, respectively. Sendai virus was highly prevalent among guinea pigs, showing so high rates positive as 80 to 100% of animals obtained from 11 of the 14 contaminated colonies. B. bronchiseptica was also shown to be prevalent within contaminated colonies by indication of rates positive more than 40% of animals examined in 14 of the 20 colonies. Infection rates of other 3 pathogens, however, ranged from lower than 20% to higher than 80% according to colonies. All strains of Salmonella isolated in this survey were identified as S. typhimurium and those of S. pneumoniae as serotype 19F.

Animals↗