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PubMed2004

A novel laccase with fair thermostability from the edible wild mushroom (Albatrella dispansus).

A laccase with a novel N-terminal sequence was purified from fresh fruiting bodies of the edible wild mushroom Albatrella dispansus using a procedure that entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel and Con A-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. In contrast to most of the previously reported laccases from mushroom mycelia, the laccase was unadsorbed on DEAE-cellulose. Although it was also unadsorbed on Affi-gel blue gel, it was adsorbed on Con A-Sepharose, indicating that it is a glycoprotein. It exhibited a molecular mass of 62kDa in gel filtration and SDS-PAGE. The activity of the laccase increased with temperature from 20 to 70 degrees C, and notably remained high at 80 degrees C. The pH optimum for the enzyme was around 4. Enzyme activity was indiscernible at pH 8 and pH 9. The laccase did not exert any inhibitory activity toward HIV-1 reverse transcriptase at a concentration of 1mg/ml, unlike some previously reported mushroom proteins.

Agaricales↗

PubMed2005

Comparative study of mycelial growth and basidiomata formation in seven different species of the edible mushroom genus Hericium.

The potential of using several agricultural by-products as supplements of sawdust substrate for the production of edible mushroom Hericium was evaluated using seven Hericium species. All the tested supplements (rice bran, wheat bran, barley bran, Chinese cabbage, egg shell, and soybean powder) were found to be suitable for the mycelial growth of all the tested species. In mycelial growth, soybean powder was the best supplement for Hericium americanum, Hericium coralloides, and Hericium erinaceum while barley bran was the best for Hericium alpestre, Hericium laciniatum, and Hericium erinaceus. For Hericium abietis, rice bran and Chinese cabbage was the best. The possibility of mushroom production on oak sawdust substrate with 20% rice bran supplement was demonstrated with H. coralloides, H. americanum, H. erinaceus, and H. erinaceum which showed 26-70% biological efficiency. Our results also showed that strain selection is important to improve biological efficiency and mushroom yield in Hericium cultivation.

Agriculture↗

PubMed2004

Evaluation of a new biosensor-based mushroom (Agaricus bisporus) tissue homogenate: investigation of certain phenolic compounds and some inhibitor effects.

A biosensor based on mushroom tissue homogenate for detecting some phenolic compounds (PCs) and usage of the biosensor for quantifying certain substances that inhibit the polyphenol oxidase activity in mushroom (Agaricus bisporus) tissue homogenate is described. The mushroom tissue homogenate was immobilized to the top of a Clark-type oxygen electrode with gelatin and glutaraldehyde. Optimization of the experimental parameters was done by buffer system, pH, buffer concentration, and temperature. Besides, the detection range of eight phenolic compounds were obtained with the help of the calibration graphs. Thermal stability, storage stability, and repeatability of the biosensor were also investigated. A linear response was observed from 20 x 10(-3) to 200 x 10(-3) mM phenol. The biosensor retained approximately 74% of its original activity after 25 days of storage at 4 degrees C. In repeatability studies, variation coefficient (C.V.) and standard deviation (S.D.) were calculated as 2.44% and +/-0.002, respectively. Inhibition studies revealed that the proposed biosensor was applicable for monitoring benzoic acid and thiourea in soft drinks and fruit juices.

Agaricus↗

PubMed2005

Structure selectivity in degradation and translocation of polychlorinated biphenyls (Delor 103) with a Pleurotus ostreatus (oyster mushroom) culture.

The biodegradation of polychlorinated biphenyls (PCBs), formerly applied in technical mixtures called Delor 103, was realized by the white rot fungus Pleurotus ostreatus. Besides the efficiency of degradation the translocation of PCBs into the fruit bodies grown during cultivation on straw were of special interest. The selectivity of PCBs degradation is discussed on the basis of structural features of the individual congeners. The most important structural factors affecting the degradation efficiency are indicated with molecular descriptors. Basic dependences of the degradation efficiency of congeners on their chlorination grade and ortho chlorosubstitution could be proved. Additionally, other structural parameters such as the number and position of non-substituted carbon in the biphenyl molecule also affect the degradation results. Apart from the removal of Delor 103 the translocation of PCB isomers into the fruit bodies growing up during cultivation was observed. The structural features assisting the transport of PCB congeners from the straw substrate colonized with mushroom mycelium (straw/mycelium) to the mushroom fruit bodies are also relevant for the degradation mechanism but act reversely. Among the chlorination grade, structural parameters such as the number of para and adversely positioned chlorines or the number of occupied para and meta positions contribute to an accumulation of individual PCB congeners in the mushrooms. In total, only few PCB amounts (below 0.1% of the initially present PCBs in straw) were transported into the fruit bodies and a dangerous PCB contamination was never reached while the experiment. A combination of accelerated solvent extraction and GC-MS analysis was the analytical basis for the investigation presented.

Biodegradation, Environmental↗

PubMed2004

Mass spectrometric determination of acromelic acid A from a new poisonous mushroom: Clitocybe amoenolens.

As Clitocybe acromelalga, the mushroom Clitocybe amoenolens is responsible for erythermalgia. Acromelic acids isolated from C. acromelalga have been suspected to be to some extend the active principles. The objective was to develop a specific and sensitive liquid chromatographic-mass spectrometric method that would allow acromelic acid A identification and quantification in mushrooms. The method involved a single-step methanol-water extraction followed by a selective cleanup of the extract with solid-phase extraction cartridges (strong-anion exchange). The chromatographic separation was achieved on a porous graphitic carbon column with acetonitrile-water-formic acid as mobile phase. Detection was done with a mass analyzer equipped with a TurboIonSpray source, operated in the negative ionization mode. Acromelic acid A concentration was determined in dried mushroom at around 325 ng/mg in C. amoenolens and 283 ng/mg in C. acromelalga.

Agaricales↗

PubMed2005

Increased plasma creatine kinase activities triggered by edible wild mushrooms.

The consumption of wild mushrooms is mostly based on tradition and not scientific evidence. Recently the widely consumed Tricholoma flavovirens caused delayed rhabdomyolysis in humans. In this study, 42 mice and 4 humans consumed T. flavovirens mixed with regular food items. In mice, the plasma creatine kinase activity increased at 9 g kg(-1)day(-1). However, the same was observed with the well-known and commercially important Boletus edulis. The observed effect is probably not species-specific but represents an unspecific response and requires individual sensitivity and a great amount of ingested mushroom to manifest itself. A screening program of wild mushrooms is recommended to clarify how widespread this effect is.

Adult↗

PubMed2006

Analysis of hallucinogenic constituents in Amanita mushrooms circulated in Japan.

The constituents of seven mushrooms sold as Amanita muscaria or Amanita pantherina (five A. muscaria and two A. pantherina) and four "extracts purported to contain A. muscaria" products that are currently circulated in Japan were determined. All mushroom samples were identified as A. muscaria or A. pantherina by macroscopic and microscopic observation. The dissociative constituents, ibotenic acid (IBO) and muscimol (MUS), were extracted with 70% methanol twice and determined by gas chromatography/mass spectrometry. The IBO (as the hydrate)/MUS contents were in the range of <10-2845ppm/46-1052ppm in the cap of A. muscaria and 188-269ppm/1554-1880ppm in the cap of A. pantherina. In the caps, these compounds had a tendency to be more concentrated in the flesh than in the cuticle. On the other hand, the IBO/MUS contents in the stem were far lower than in the caps. In the "extracts purported to contain A. muscaria" products, IBO/MUS were detected below the lower limit of calibration curve (<10ppm/<25ppm) or not detected. However, these samples contained other psychoactive compounds, such as psychoactive tryptamines (5-methoxy-N,N-diisopropyltryptamine and 5-methoxy-N,N-dimethyltryptamine), reversible monoamine oxidase inhibitors (harmine and harmaline) and tropane alkaloids (atropine and scopolamine), which were not quantified. This is the first report of the chemical analysis of Amanita mushrooms that are circulated in the drug market.

Amanita↗

PubMed2005

Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea.

Studies have shown that certain foods contain compounds with antigenotoxic activities. Here, we ask if dried powders and/or extracts from three edible mushrooms, Agrocybe cylindracea, Lentinula edodes and Pleurotus ostreatus, have a mitigating effect on genotoxicity. We used two in vivo assays: the Drosophila DNA repair test and the Drosophila wing spot test (also known as SMART) which measures somatic mutation and recombination. Eight carcinogens were tested with the mushroom powders: 2-AAF, aflatoxin B1, DMBA, IQ, MeIQx, MNU NDMA, and 4NQO. We found that A. cylindracea and P. ostreatus powders can suppress DNA damage induced by each of the mutagens we tested. In contrast, L. edodes has an inhibitory effect on DNA damage induced by only a sub-set of mutagens, namely aflatoxin B1, NDMA, MNU and 4NQO. In addition, A. cylindracea extracts were able to suppress somatic cell mutation induced by aflatoxin B1, MMC, MNU, NDMA, NMOR and 4NQO. These results suggest that Agrocybe genus mushrooms contain factors with antigenotoxic activity, including anti-recombinogenic activity. Furthermore, the antigenotoxic activity of A. cylindracea powder can be extracted in water but not in ethyl acetate or methanol, and is sensitive to heat treatment. The data suggest that there is a novel antigenotoxic factor(s) in A. cylindracea, possibly in the form of a peptide or protein.

Agaricales↗

PubMed2006

Fatty acid composition of lipids from mushrooms belonging to the family Boletaceae.

The non-polar lipid content and fatty acid (FA) composition of 11 mushroom species of the family Boletaceae were determined. The non-polar lipid content ranged from 2.0 (Leccinum aurantiacum and Boletus erythropus) to 5.4 % (w/w) d.w. (Suillus grevillei) with an average value of 2.9 %. More than 25 different FAs were found in the mushroom lipids. Unsaturated FAs, mainly linoleic and oleic acids, accounted for about 83 % of the total FAs, while palmitic acid was the main saturated FA. Some FAs are identified for the first time in Boletaceae and in higher Basidiomycetes (cis-11,12-methyleneoctadecanoic acid, 7-cis,10-cis hexadecadienoic) or in fungi (cis-11,12-methyleneoctadecanoic acid). There were significant differences (P<0.05) in the contents of specific FAs between mushroom species.

Agaricales↗

PubMed2004

A novel ribonuclease from fruiting bodies of the common edible mushroom Pleurotus eryngii.

A ribonuclease with a temperature optimum of about 70 degrees C and a pH optimum of 6.5 was isolated from fruiting bodies of the mushroom Pleurotus eryngii. The ribonuclease was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and S-Sepharose. It possessed a molecular mass of 16 kDa, and exhibited higher ribonucleolytic activity toward poly A and poly G and lower ribonucleolytic activity toward poly C and poly U. Its N-terminal sequence was distinctly different from those of other mushroom ribonucleases, and resembled that of Pleurotus tuber-regium only by 40%. Furthermore, its thermostability characteristics, polyhomoribonucleotide specificity and molecular mass were dissimilar to those of other mushroom ribonucleases.

Amino Acid Sequence↗

PubMed2004

Beta-adenosine, a bioactive compound in grass chaff stimulating mushroom production.

Fructification and yield of the edible mushrooms Pleurotus pulmonarius and Stropharia rugosoannulata are clearly enhanced when wheat straw is supplemented with 30% Lolium perenne grass chaff. The bioactive compound in the methanol extract of grass chaff was identified as beta-adenosine. In vitro biological activity tests showed that 0.012 mg of beta-adenosine per ml of medium stimulated earlier fructification of Pleurotus pulmonarius. Mushroom fruiting trials showed that when 12 mg beta-adenosine was added to 1 kg wet wheat straw, primordia of Pleurotus pulmonarius appeared two days earlier and primordia of Stropharia rugosoannulata appeared 18 days earlier when compared to pure wheat straw substrate. This concentration of beta-adenosine had no impact on the mushroom yield of Pleurotus, but resulted in a 2.2 fold increase in yield for Stropharia. beta-Adenosine at 25 mg per kg wet wheat straw increased the yield of Pleurotus with 52% and the yield of Stropharia with 258%, but this concentration delayed primordial formation in Pleurotus.

Adenosine↗

PubMed1996

Mushroom-shaped choroidal hemangioma.

PURPOSE: We examined the clinicopathologic features of a mushroom-shaped choroidal tumor that originated in the right eye of a 13-year-old girl. METHODS: The clinical, ultrasonographic, and histopathologic features of the enucleated eye were studied. RESULTS: On clinical examination, the choroidal tumor appeared mushroom-shaped with ultrasonic characteristics of high internal reflectivity and choroidal excavation. The enucleation specimen contained a cavernous hemangioma that extended through Bruch's membrane. CONCLUSION: Choroidal hemangioma may extend through Bruch's membrane and be mushroom shaped.

Adolescent↗

PubMed1997

Free radical scavenging activities of mushroom polysaccharide extracts.

The superoxide and hydroxyl radical scavenging activities of eight mushroom antitumor polysaccharide extracts were investigated using phenazin methosulphate-NADH-nitroblue tetrazolium system and ascorbic acid-Cu(2+)-cytochrome C system respectively. The results showed that six of eight mushroom polysaccharide extracts had superoxide and hydroxyl radical scavenging activities. The protein content of the polysaccharide extracts appeared to contribute a direct effect on free radical scavenging activity. However, none of the mushroom polysaccharide extracts had antioxidative activity as measured by detecting malondialdehyde (MDA) contents of liver microsomes.

Antineoplastic Agents↗

PubMed1997

The concentrations and bioconcentration factors of mercury in mushrooms from the Mierzeja Wiślana sand-bar, northern Poland.

The total concentration of mercury was determined in 15 species of wild growing mushrooms, each represented by 15 specimens including underlying substrate, collected from the unpolluted area of the sand-bar Mierzeja Wiślana in the northern part of Poland in 1993-1994. Mercury was measured by cold vapour atomic absorption spectrometry (CV-AAS) after wet digestion of the samples with concentrated nitric acid in a glass system. The mushroom species examined showed a wide range of mercury concentrations, i.e. from 5.6 +/- 2.1 micrograms/g dry wt. in Scleroderma citrinum to 1100 +/- 240 micrograms/g in the edible Macrolepiota procera. No bioconcentration of mercury was observed in Xerocomus badius, Paxilus involutus, Russula queletii and Lactarius rufus. In Hygrophorius aurantiaca, Armilariella mellea and Amanita vaginata the bioconcentration factor (BCF) values of mercury were close to one. Accumulation of mercury was slight in Oudemansiella platyphylla and Amanita citrica (BCF between 2.0 and 3.3 in the caps and 1.7 in the stalks) and high in Leccinum scabrurm, Amanita muscaria and Macrolepiota procera (BCFs between 11 and 35 in the caps and 6.7-18 in the stalks). In the whole fruiting body of Polyporus melanopsuss and Scleroderma citrinum the BCF values of mercury were 4.4 and 0.083, respectively. There was a high correlation between the concentrations of mercury in the caps and stalks of Macrolepiota procera and in the underlying substrate (P < 0.001), a slight correlation for Polyporus melanopsuss, and also for the caps of Leccinum scabrum, Oudemansiella platyphylla and Russula aeruginea and the stalks of Hygrophoropsis aurantiaca (P < 0.01) and L. scabrum (P < 0.05). For the other mushroom species investigated there was no correlation between mercury concentration in the fruiting bodies and underlying substrate.

Basidiomycota↗

PubMed1998

Eosinophilia and gastrointestinal symptoms after ingestion of shiitake mushrooms.

BACKGROUND: Shiitake mushrooms are a dietary staple in Asia and are increasingly popular worldwide. A cholesterol-lowering study with shiitake showed that 17 of 49 participants withdrew because of rash or abdominal discomfort, and two had marked eosinophilia. One of these latter participants was subsequently challenged for 14 days with shiitake powder and again had eosinophilia. OBJECTIVE: We investigated whether ingestion of shiitake mushroom powder induces eosinophilia or symptoms. METHODS: We studied 10 normal persons. Each participant ingested 4 gm shiitake powder (open label) daily for 10 weeks (trial 1), and the protocol was repeated in these same subjects after 3 to 6 months (trial 2). Blood counts and serum samples were obtained biweekly (trial 1) or weekly along with stool specimens (trial 2). Eosinophil major basic protein and IL-5, IgE, and IgG antishiitake antibodies were measured in sera. Eosinophil-derived neurotoxin was measured in stool extracts. We defined responders as subjects having peak eosinophil counts four or more times their average baseline counts. RESULTS: Each trial had four responders, and trial 2 had one new and three repeat responders. Eosinophilia ranged from 400 to 3900/mm3. Responders had increased blood eosinophils, serum major basic protein, stool eosinophil-derived neurotoxin, and factors that enhanced eosinophil viability. Antishiitake IgE was not detected, and antishiitake IgG increased in two responders. Gastrointestinal symptoms coincided with eosinophilia in two subjects. Symptoms and eosinophilia resolved after discontinuing shiitake ingestion. CONCLUSIONS: Daily ingestion of shiitake mushroom powder in five of 10 healthy persons provoked blood eosinophilia, increased eosinophil granule proteins in serum and stool, and increased gastrointestinal symptoms. Shiitake ingestion suggests a model to study the eosinophil's role in the blood and gastrointestinal tract. Finally, our report raises concerns of possible adverse systemic reactions to this increasingly popular food.

Adult↗

PubMed2001

Effect of captopril on mushroom tyrosinase activity in vitro.

The study presented here demonstrates that the antihypertensive drug captopril ([2S]-N-[3-mercapto-2-methylpropionyl]-L-proline) is an irreversible non-competitive inhibitor and an irreversible competitive inhibitor of the monophenolase and diphenolase activities of mushroom tyrosinase when L-tyrosine and L-DOPA were assayed spectrophotometrically in vitro, respectively. Captopril was rendered unstable by tyrosinase catalysis because of the interaction between the enzymatic-generated product (o-quinone) and captopril to give rise to a colourless conjugate. Therefore, captopril was able to prevent melanin formation. The spectrophotometric recordings of the inhibition of tyrosinase by captopril were characterised by the presence of a lag period prior to the attainment of an inhibited steady state rate. The lag period corresponded to the time in which captopril was reacting with the enzymatically generated o-quinone. Increasing captopril concentrations provoked longer lag periods as well as a concomitant decrease in the tyrosinase activity. Both lag period and steady state rate were dependent of captopril, substrate and tyrosinase concentrations. The inhibition of both monophenolase and diphenolase activities of tyrosinase by captopril showed positive kinetic co-operativity which arose from the protection of both substrate and o-quinone against inhibition by captopril. Inhibition experiments carried out using a latent mushroom tyrosinase demonstrated that captopril only bound the enzyme at its active site. The presence of copper ions only partially prevented but not reverted mushroom tyrosinase inhibition. This could be due to the formation of both copper-captopril complex and disulphide interchange reactions between captopril and cysteine rich domains at the active site of the enzyme.

Agaricales↗

PubMed2002

A ubiquitin-like peptide from the mushroom Pleurotus sajor-caju exhibits relatively potent translation-inhibitory and ribonuclease activities.

The fruiting bodies of the edible mushroom Pleurotus sajor-caju were extracted with an aqueous buffer and then subjected to affinity chromatography on Affi-gel Blue gel, ion exchange chromatography on DEAE-cellulose and gel filtration on Superdex 75. From the fraction of the extract adsorbed on Affi-gel Blue gel and unadsorbed on DEAE-cellulose, a 9.5 kDa peptide with an N-terminal sequence similar to ubiquitin was isolated with a yield of 0.25 mg/kg mushroom. The peptide inhibited cell-free translation with an IC(50) of 30 nM. It exhibited a ribonuclease activity of 450 U/mg toward yeast transfer RNA. The activities were substantially more potent than those of previously isolated mushroom ubiquitin-like protein and peptide.

Fungal Proteins↗

PubMed2002

Anti-genotoxic effect of aqueous extracts of sun mushroom (Agaricus blazei Murill lineage 99/26) in mammalian cells in vitro.

The "sun mushroom" is the popular name for the Agaricus blazei Murill fungus, a mushroom native to south-eastern Brazil, which has been frequently used in popular medicine mainly in the form of tea to treat various ailments (stress, diabetes, etc.). In the present study, the genotoxic and/or anti-genotoxic effects ofA. blazei on mammalian cells in culture was assessed by checking the increase or reduction of micronucleus (MN) frequency and comets. The sun mushroom (lineage 99/26) was used as aqueous extracts prepared (2.5%) at three different temperatures (60, 25 and 4 degrees C). The in vitro micronucleus (MN) test in binucleated cells and comet assay were used in V79 cells cultivated in HAM-F10+DMEM medium (1:1), supplemented with 10% of fetal bovine serum. The experiments were divided into four treatment types: 1. Negative control; 2. Positive control with MMS; 3. Treatments with the three forms of extracts (60, 25 and 4 degrees C); and 4. Treatments with the extracts in different associations (simultaneous, pre-treatment, post-treatment and simultaneous after pre-incubation for 1 h) with MMS. None of the A. blazei extracts show genotoxic activity. In the comet assay no protecting effect was found. The results obtained in the MN test showed that the three forms of extracts used had protective activity, suggesting that the compound or active ingredients of A. blazei are always present in these extracts. The greater protective efficiency of the simultaneous treatment and simultaneous treatment with pre-incubation mixture with MMS suggests that the extracts have an antimutagenic action of the desmutagenic type.

Agaricus↗