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At least 469 records · Page 26Linked to original sources
PubMed2003

Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows.

A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.

Animals↗
PubMed1982

Modification of nucleic acids with muta-carcinogenic heteroaromatic amines in vivo. Identification of modified bases in DNA extracted from rats injected with 3-amino-1-methyl-5H-pyrido[4,3-b]indole and 2-amino-6-methyldipyrido[1,2-a3:3',2'-d]imidazole.

Potent muta-carcinogens isolated from amino-acid pyrolysates, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-1-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), were injected intraperitoneally into Male Wistar rats. DNA and rRNA modified with these muta-carcinogens were then extracted from the livers of the rats, and modified nucleic-acid bases were isolated and analysed by high-performance liquid chromatography (HPLC). These modified nucleic-acid bases were identified as 3-(C8-guanyl)amino-1-methyl-5H-pyrido[4,3-b]indole (Gua-Trp-P-2) and 2-(C8-guanyl)amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Gua-Glu-P-1) by comparison of their retention times on HPLC and UV spectra with those of authentic compounds.

Animals↗