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At least 469 records · Page 26Linked to original sources
PubMedDate not supplied

Modification of DNA patterns in plasma and nucleated blood cells from systemic sclerosis patients.

OBJECTIVE: To analyze the DNA patterns extracted from plasma and nucleated blood cells (lymphocytes) in systemic sclerosis (SSc) with a new MFC DNA extracting kit. METHODS: Ten SSc patients and 9 healthy controls were studied. Heparin containing blood samples were separated into plasma and buffy coat fractions and subjected to DNA extraction. The DNA pattern was revealed by 0.4% agarose electrophoresis and analyzed in a Gelblot Programme file (UVP Product). RESULTS: In control samples the DNA pattern observed in plasma extract was different from that of the buffy coat. For the plasma a series of peaks ranging from 2-23 Kb were present, and for the buffy coat we usually observed 2 to 3 principle bands, respectively, at around 33 Kb and 0.5 Kb. For SSc patients the DNA patterns that resulted from the plasma and buffy coat were totally different from the control samples, with some exceptions. CONCLUSION: We observed that SSc samples contain a distinctively different DNA pattern compared to healthy controls. Further studies are needed to establish whether or not this DNA pattern might be considered peculiar to SSc, and whether or not the method is a useful tool for pathogenic studies of the disease and for diagnostic purposes.

Adult↗
PubMedDate not supplied

[The kinetics of the chemical modification of DNA radiation transformations in multicomponent systems. An analytical review].

Most probable ways of chemical protection and sensitization of DNA macromolecules under the effect of ionizing radiation in aqueous solutions have been investigated. A kinetic model in proposed, based on the experimental data available, that consists of 25 reactions involving DNA (D), protein (P), glutathione (G), oxygen (O2), various protectors and sensitizers (A), active products of water radiolysis (O, e (H)), free and peroxide radicals of DNA and other components, and peroxides. General and partial rates of radiation transformations of DNA, depending on D, P, G, O2 and A concentrations are expressed in analytical terms. The relationships obtained are used for a qualitative description of some biological effects of ionizing radiation that is: a difference in radiosensitivity of different organs and tissues and its alteration during the cell cycle; oxygen effect; and the concentration relationships of the radioprotection and radiosensitization coefficients.

DNA↗
PubMed1991

[Modification of DNA typing of blood stains by textile stain carriers].

Samples of 20 microliters blood were applicated von 55 different textiles, containing all usual materials for clothes, straight from the fabric and after thorough washing. DNA profiling was influenced only by blue jeans and blue terry towel straight from the fabric; in some of these samples there was an inhibition of the restriction enzyme digest that could not be prevented by an additional dialysis step.

ABO Blood-Group System↗
PubMed1990

Modification of DNA repair by human interferons.

We have found a new biological function of interferons, namely, their capacity to protect human cells from the action of some physical and chemical mutagens. To evaluate the protective effect of interferons the following criteria were applied: formation of sister chromatid exchanges (SCE) and chromosomal aberrations (CA), as well as viability of cells and intensity of DNA repair synthesis. Pretreatment of cells with natural interferon decreased the number of sister chromatid exchanges and chromosomal aberrations, induced by different mutagens, and increased the intensity of DNA repair synthesis. This is attributed to the ability of interferon to enhance certain phases of DNA repair. In the case of photomutagenic action of 8-methoxypsoralen (8-MOP) on the lymphocytes, when monoadducts (MA) only, or both monoadducts and interstrand cross-links (ICL) are formed, the antimutagenic effect of interferon is exhibited only with respect to ICL. Unlike the natural interferon, the recombinant alpha 2-interferon failed to have any effect on the lymphocytes of clinically healthy donors exposed to gamma-radiation. In the repair- deficient cells (Marfan's syndrome) the protection of natural interferon against the action of 4-nitroquinoline-1'-oxide and gamma- radiation was found to be reduced significantly and that of alpha 2-interferon was not manifested at all. Thus, the capacity of interferons to alter the DNA repair, conceivably, depends on the type of interferon and on the cell genotype.

4-Nitroquinoline-1-oxide↗
PubMedDate not supplied

[Modification of DNA damage in rat hepatocytes following whole-body roentgen radiation using cysteamine and 8-bromocaffeine].

Using the elastoviscosimetric method it was shown that the level of residual DNA double-strand breaks in rat hepatocytes depended on conditions of total-body X-irradiation: it increased in the presence of a sensitizer, 8-bromocaffeine and decreased in the presence of a protector, cysteamine. This correlation confirms a major role of fixed DNA double-strand breaks in the formation of a lethal effect in a cell.

Animals↗