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Stability and change in mothers' internal representations of their infants over time.

This study examined predictors of stability and change in women's maternal representations of their children. Participants were 180 women, recruited from the community, half of whom had experienced domestic violence during pregnancy. Maternal representations of were assessed with the Working Model of the Child Interview (WMCI; Zeanah, Benoit, Hirshberg, Barton, & Regan, 1994) during the last trimester of pregnancy and again at the child's first birthday. Results indicated that when collapsed into balanced and non-balanced categories, 71% of the sample was stable over time, and women who had balanced representations had significantly more stable representations than women who had non-balanced representations (p < .001). Income, single parenthood, abuse status, and depressive symptomatology predicted change. In addition, women who became non-balanced postnatally benefited from having balanced representations while pregnant to buffer the quality of their interactions with their children.

Adolescent↗

Parental representations, self-view, and interpersonal functioning of older adolescents.

This study examines the relationship between parental object representations, self-representations, and interpersonal relations in a nonclinical sample of older adolescents. It was hypothesized that individuals with parental representations that are conceptually low, hostile, and rejecting would be significantly related to a negative view of self and poor interpersonal relations. The results suggest that parental benevolence, ambitiousness, and conceptual level are significantly related to interpersonal functioning. Parental representations that are qualitatively poor were found to be associated with a negative view of self. However, a high conceptual level of mother was noted to be significantly related to a negative self-representation. The findings generally confirm the nature of the relationship between object representations and self, but also suggest that the structural component of parental representations may reflect a cognitive component of the self rather than an affective one. The difficulties of controlling for depression and method variance issues are also discussed.

Adolescent↗

Masters of the universe: children's toys as reflections on contemporary psychoanalytic theory.

The emergence of contemporary object relations theory and self psychology as potent theoretical forces has led to rich discussion with far-reaching implications for clinical, developmental, and metapsychological theory. This article examines these developments using as a medium the Masters of the Universe figures and stories, which have captured the imaginations of a broad spectrum of phallic-oedipal and oedipal-aged children. A series of interrelated theoretical questions will be discussed: (1) the nature and character of aggressive drives and whether these are innate and instinctual or disintegration products secondary to emphatic failure; (2) the extent of internalization of mental representations at this point in development; and (3) the status of Kohut's "grandiose self" and "idealized object" vis-à-vis traditional perspectives on phallic-oedipal and oedipal development.

Aggression↗

[Psychosomatic medicine--the future of an illusion].

This paper presents the phenomenology and the development of theories about alexithymia, a "behavioral neurosis" in psychosomatic patients, with background of the american (F. Alexander, R. Grinker and P. Knapp) and french (P. Marty) psychosomatic schools. Luminet's understanding comprises the combination of an object-relations-theory perspective of alexithyma with the clinical theory of "depréssion essentielle" of Marty. Primary and secondary shortcomings and dysregulations in the early mother-child-relation lead to essential insecurity in building up stable self and object representations with a specifically reduced libidinal tonus of the self and dependency of objects. These conditions lead to the formation of alexithymic disturbances in thought, interaction and object relations, as well as to impaired capacity for self reflection in perception and evaluation of affects, which do not become available as signals for the ego-functions. Moreover, as the capacity for self regulation, as a function of maternal care, could not be internalized, there is an increased proneness for somatisation. In literature, these characteristics were commonly taken for the main limitations for the success of psychoanalytic or psychodynamic treatments. Luminet's psychotherapeutic access in the treatment of alexithymic patients is based on activation and differentiation of the self and object representations through verbal and nonverbal techniques, aiming on actualisation and development of maternal functions; this implies the acknowledgement of the physical body within the therapeutic relationship and awareness of specific counter-transference reactions.

Affective Symptoms↗

An in vitro rapid-turnover assay for human immunodeficiency virus type 1 replication selects for cell-to-cell spread of virus.

We have developed a rapid-turnover culture system where the life span of a human immunodeficiency virus type 1-infected cell is controlled by periodic addition of a cytotoxic agent, mitomycin C. These mitomycin C-exposed cells are cocultured with a constant number of uninfected cells as new targets for the virus. Passage of the virus-infected cells under these conditions led to the emergence of a viral variant that was able to replicate efficiently in this culture system. After biologic and molecular cloning, we were able to identify a single frameshift mutation in the vpu open reading frame that was sufficient for growth of the mutant virus in the rapid-turnover assay. This virus variant spread more efficiently by cell-to-cell transfer than the parental virus did. Electron micrographs of cells infected with the delta vpu virus revealed a large number of mature viral capsids attached to the plasma membrane. The presence of these mature virus particles on the cell surface led to enhanced fusion and formation of giant syncytia with uninfected cells. Enhanced cell-to-cell transfer of the delta vpu virus provides an explanation for the survival of this mutant virus in the rapid-turnover culture system. The in vitro rapid-turnover culture system is a good representation of the in vivo turnover kinetics of infected cells and their continual replacement by host lymphopoietic mechanisms.

Amino Acid Sequence↗

The Fcgamma receptor genotype as a risk factor for generalized early-onset periodontitis in Japanese patients.

BACKGROUND: Genetic polymorphisms of immunoglobulin G (IgG) Fc receptors (FcgammaR) were recently shown to be associated with recurrence rates of adult periodontitis (AP). The purpose of this study was to evaluate whether FcgammaR polymorphisms are also associated with generalized early-onset periodontitis (G-EOP) in Japanese patients. METHODS: Thirty-eight Japanese patients with G-EOP and 83 Japanese patients with AP were identified according to established clinical criteria, including measurements of probing depth, clinical attachment level, and alveolar bone level. FcgammaR genotypes for 3 bi-allelic polymorphisms were determined in these G-EOP and AP patients and 104 race-matched healthy controls by means of allele-specific polymerase chain reactions. RESULTS: There was a significant difference in the distribution of FcgammaRIIIb genotypes between G-EOP patients and healthy controls (P = 0.02). Additionally, a significant over-representation of FcgammaRIIIb-NA2 allele was observed in G-EOP patients as compared to AP patients and controls (P= 0.02, P= 0.009, respectively). Moreover, we found a strong association between G-EOP and the composite genotype comprising FcgammaRIIIb-NA2 and FcgammaRIIIa-158F (G-EOP versus controls: odds ratio 2.4, 95% CI 1.0-6.0, chi2 = 4.13, P= 0.04). CONCLUSIONS: This study indicates that the FcgammaRIIIb-NA2 allele and possibly FcgammaRIIIa-158F could be associated with susceptibility to G-EOP in Japanese patients.

Adult↗

Trauma and the development of borderline personality disorder.

Prolonged and severe trauma, particularly trauma that occurs early in the life cycle, tends to result in a chronic inability to modulate emotions. When this occurs, people can mobilize a range of behaviors that are best understood as attempts at self-soothing. Some of these attempts include clinging and indiscriminate relationships with others in which old traumas are re-enacted over time, as well as more self-directed behaviors such as self-mutilation, eating disorders, and substance abuse. Usually, these behaviors will coexist. Patients with complicated trauma histories often repetitively attempt suicide or engage in chronic self-destructive behavior, and need to address issues of childhood trauma, neglect, and abandonment, both in the past and as re-experienced in current relationships. When treating these patients, therapists must anticipate that painful affects related to interpersonal safety, anger, and emotional needs may give rise to dissociative episodes, which may, in turn, be accompanied by increased self-destructive behavior. The therapy must clarify how current stresses are experienced as a return of past traumas and how small disruptions in present relationships are seen as a repetition of prior abandonment. As part of this, it is essential that the therapist provide validation and support, and avoid participating in a re-enactment of the trauma. Fear needs to be tamed in order for people to be able to think and be conscious of current needs. This bodily response of fear can be mitigated by safety of attachments, security of meaning schemes, and by a body whose reactions to environmental stress can be predicted and controlled. One of the great mysteries of the processing of traumatic experience is that as long as the trauma is experienced as speechless terror, the body continues to keep score and react to conditioned stimuli as a return of the trauma. When the mind is able to create symbolic representations of these past experiences, however, there often seems to be a taming of terror, a desomatization of experience. As Ducey and van der Kolk found in the Rorschachs of Vietnam veterans, patients were unresponsive to outside influences (good or bad) as long as they remained in a state of psychic numbing. Faced with intrusions of past trauma in their current emotional life, patients' initial sense of being overwhelmed was mastered only when a link between past trauma and current perceptions became understood.(ABSTRACT TRUNCATED AT 400 WORDS)

Borderline Personality Disorder↗

Further data on the effects of subliminal symbiotic stimulation on schizophrenics.

This investigation further examined the effects of activating unconscious symbiotic fantasies in schizophrenics. One hundred twenty-eight hospitalized schizophrenic men who qualified as "relatively differentiated" on the Adjective Rating Scale were randomly assigned to four groups. Each group was assessed for pathological thinking, pathological nonverbal behavior, and self-esteem before and after the subliminal exposure of an experimental and control stimulus. The control stimulus for all groups was the message PEOPLE ARE WALKING and the experimental stimuli were the messages MOMMY AND I ARE ONE, MOMMY IS ALWAYS WITH ME, MOMMY FEEDS ME WELL, and I CANNOT HURT MOMMY (one for each group). One half of each group was subliminally exposed to verbal messages only and one half to verbal messages accompanied by congruent pictures. The first stimulus (MOMMY AND I ARE ONE) was intended to activate unconscious symbiotic fantasies that in a number of prior studies reduced pathology in groups of relatively differentiated schizophrenics. The other stimuli were intended to activate reassuring unconscious fantasies about "mommy" that were not specifically symbiosis-related. Only the MOMMY AND I ARE ONE stimulus led to more adaptive behavior and did so on all three dependent variables. This supported the supposition, also borne out in two other studies, that it is specifically symbiosis-related gratifications that are ameliorative for schizophrenics. The above results were considerably stronger for the subgroup that was exposed to a picture accompanying the MOMMY AND I ARE ONE message. This was viewed as probably the result of the pictorial representation serving as a concretization of the more abstract verbal message and as such being more relevant to the relatively primitive mode of thinking in schizophrenia.

Adolescent↗

Malevolence, splitting, and parental ratings by borderlines.

Malevolent object relations as well as splitting have long been considered by psychodynamic theorists as central features of borderline personality disorder. We tested the hypotheses that borderlines would a) perceive their parents more negatively than both nonborderline major depressive patients and nonpatient normal controls, and b) split their representations of their parents into opposites more than the comparison subjects. Borderlines (N = 31), who were identified by the Diagnostic Interview for Borderlines, Research Diagnostic Criteria major depressives (N = 15), and nonpatient controls (N = 14) were asked to rate each parent on the Adjective Check List (ACL; Gough and Heilbrun, 1983). Seven ACL scales were studied: Favorable, Unfavorable, Critical Parent, Nurturing Parent, Nurturance, Aggression, and Dominance. Correlations were performed between scores for mother and father on the various scales for each of the three cohorts. Analysis of variance and one-way t-tests with Bonferroni correction were used to test group differences. Borderlines rated their parents, especially their fathers, not only as more unfavorable on negative scales than depressives or normals, but as less favorable on positive scales than the comparison groups. Analysis of covariance revealed that a significant portion of the variance in father scores, but not in mother scores, was related to age of respondent and history of sexual abuse. While borderlines did not appear to split their parents into one good and one bad parent, they did show significantly less correlation between parents on the Favorable scale when compared with either depressives or normal subjects. The results imply that borderlines have a greater tendency to view the world in negative, malevolent ways than to split their object representations.

Adult↗

An estimation of a model explaining the mechanism causing neurotic disorders using a theory of fuzzy sets.

Constructing and estimating a model to explain the mechanism of neurotic disorders is important and significant. The model helps the rearrangement or representation of knowledge obtained from professional physicians. However, it is a very difficult problem, because the objects requiring analysis are mental activities of human beings, and they originally include a comparatively large variance between individuals. The object data were obtained from patients with neurotic disorders who were diagnosed by several doctors for 10 years in a subagricultural areas in Japan. We analyzed the data and calculated the weights attached for personal information depending upon the similarity between the information and the kinds of neurotic disorders using the theory of fuzzy sets. From the results of our analysis, we constructed and estimated a model explaining the mechanism causing neurotic disorders as several linear equations. From data processing points of view, the estimation we attempted is placed in a kind of effective data compression with respect to discrete statistical data.

Adult↗

Amplification in the leader sequence of late polyoma virus mRNAs.

Ribonuclease T1 fingerprints of the three "late" polyoma virus mRNAs show that oligonucleotides of the leader sequence are present in multiple copies in each mRNA. These oligonucleotides, however, appear unimolar in fingerprints of complete, continuous transcripts of the late strand of the viral DNA. Oligonucleotides which are represented only once in the DNA are thus reiterated in the mature mRNAs. Consequently, when mRNA was hybridized to the leader region of immobilized viral DNA, those copies present in excess of their genomic representation failed to hybridize and were released by RNAase treatment. Analysis of the RNAase-resistant hybrids revealed a series of leader species with complex sequence arrangements. We suggest that these complicated reiterated sequences are generated during the processing of a precursor RNA which extends several times around the genome. This RNA would be shortened by a series of splicing reactions which conserve sequences from the leader region and attach them to a suitable coding sequence.

Animals↗

Resonant mirror biosensor detection method based on yessotoxin-phosphodiesterase interactions.

Yessotoxin (YTX) is a generic name for a group of lipophilic compounds recently discovered and chemically characterized. Association measurements were done in a resonant mirror biosensor. The instrument detects changes in the refractive index and/or thickness occurring within a few hundred nanometers form the sensor surface where a molecule is attached. We used aminosilane surfaces where phosphodiesterase 3',5'-cyclic-nucleotide-specific from bovine brain (PDEs) was immobilized. Over this immobilized ligand different amounts of YTX were added and typical association curve profiles were observed. These association curves fit a pseudo-first-order kinetic equation where the apparent association rate constant (k(on)) can be calculated. The value of this constant increases with YTX concentration. From the representation of k(on) versus YTX concentration we obtained the association rate constant (k(ass)) 248+/-40 M(-1)s(-1) and the dissociation rate constant (k(diss)) 9.36 x 10(-4)+/-1.72 x 10(-4)s(-1). From these values the kinetic equilibrium dissociation constant (K(D)) for YTX-PDEs association can be calculated. The value of this last constant is 3.74 x 10(-6)+/-8.25 x 10(-8)M YTX. The PDE-YTX association was used as a method suitable for determination of the toxin concentration in a shellfish sample. The assay had sufficient sensitivity and can be used on simple shellfish extracts.

3',5'-Cyclic-AMP Phosphodiesterases↗

Potential energy surfaces for small alcohol dimers I: methanol and ethanol.

Potential energy landscapes for homogeneous dimers of methanol and ethanol were calculated using counterpoise (CP) corrected energies at the MP26-311+G(2df,2pd) level. The landscapes were sampled at approximately 15 dimer separation distances for different relative monomer geometries, or routes, given in terms of a relative monomer yaw, pitch, and roll and the spherical angles between the monomer centers (taken as the C atom attached to the O). The 19 different routes studied for methanol and the 22 routes examined for ethanol include 607 CP corrected energies. Both landscapes can be adequately represented by site-site, pairwise-additive models, suitable for use in molecular simulations. A modified Morse potential is used for the individual pair interactions either with or without point charges to represent the monomer charge distribution. A slightly better representation of the methanol landscape is obtained using point charges, while the potential energy landscape of ethanol is slightly better without point charges. This latter representation may be computationally advantageous for molecular simulations because it avoids difficulties associated with long-range effects of point-charge-type models.

Journal Article↗

Adolescents' working models and styles for relationships with parents, friends, and romantic partners.

This study examined the links among adolescents' representations of their relationships with parents, friends, and romantic partners. Sixty-eight adolescents were interviewed three times to assess their working models for each of these types of relationships. Working models of friendships were related to working models of relationships with parents and romantic partners. Working models of relationships with parents and romantic partners were inconsistently related. A similar pattern of results was obtained for self-report measures of relational styles for the three types of relationships. Perceived experiences were also related. Specifically, support in relationships with parents tended to be related to support in romantic relationships and friendships, but the latter two were unrelated. On the other hand, self and other controlling behaviors in friendships were related to corresponding behaviors in romantic relationships. Negative interactions in the three types of relationships also tended to be related. Taken together, the findings indicate that the representations of the three types of relationships are distinct, yet related. Discussion focuses on the nature of the links among the three.

Adolescent↗

Spin-flip symmetry and synchronization.

In the context of optimization by evolutionary algorithms (EAs), epistasis, deception, and scaling are well-known examples of problem difficulty characteristics. The presence of one such characteristic in the representation of a search problem indicates a certain type of difficulty the EA is to encounter during its search for globally optimal configurations. In this paper, we claim that the occurrence of symmetry in the representation is another problem difficulty characteristic and discuss one particular form, spin-flip symmetry, characterized by fitness invariant permutations on the alphabet. Its usual effect on unspecialized EAs, premature convergence due to synchronization problems, is discussed in detail. We discuss five different ways to specialize EAs to cope with the symmetry: adapting the genetic operators, changing the fitness function, using a niching technique, using a distributed EA, and attaching a highly redundant genotype-phenotype mapping.

Algorithms↗

[Deglutition anxiety. Consideration of symptoms and psychodynamics of heart phobia].

Fear of swallowing as an accessory symptom or symptomatical precursor of heart-phobic development is pointed out along two short casuistic reports. In highly regressive states fearful inhibition of the act of swallowing proper occurred in relation to food and even own saliva. The symptom is discussed before the background of separation conflicts and pathological internalization processes with confusion of self- and object-representations in heart-phobic patients. The author argues that an understanding of this symptom provides insight into the--inspite of phobic phenomenology--essentially hypochondriac psychodynamics of heart-phobic patients with narcissistic traits in particular.

Adult↗

Rapid screening for HLA-B27 by a TaqMan-PCR assay using sequence-specific primers and a minor groove binder probe, a novel type of TaqMan trade mark probe.

HLA-B27 is strongly associated with ankylosing spondylitis (AS). As typing for HLA-B27 is routinely performed by serological methods, false-positive results can be generated. Therefore, several more accurate molecular methods have been developed for HLA-B27 genotyping. We describe a real-time PCR method for the detection of the HLA-B27 allele using sequence-specific primers (SSP) combined with a fluorogenic MGB probe (minor groove binder probe), a novel type of TaqMan probe. The MGB increases the melting temperature (T(m)) of the probe, allowing the use of shorter probes. Moreover, the use of a non-fluorescent quencher (NFQ) attached to the MGB probe improves the efficiency of fluorescence quenching, thus providing a very low fluorescent background. We tested this method on 150 subjects (41 HLA-B27 positive and 109 HLA-B27 negative) who underwent routine HLA-B27 serological testing by flow cytometry (FC). Serology and our TaqMan assay gave identical results in all cases and no false positive or negative results were observed. A graphical representation of fluorescence and normalized reporter signal (DeltaRn) values demonstrated that HLA-B27 positive and HLA-B27 negative samples formed two tight clusters making it possible to clearly differentiate between HLA-B27 positive and negative samples. This single tube PCR method for the detection of HLA-B27 should be particularly suitable for the routine analysis of large numbers of samples in the laboratory.

DNA Primers↗

Virtually all methicillin-resistant Staphylococcus aureus (MRSA) infections in the largest Portuguese teaching hospital are caused by two internationally spread multiresistant strains: the 'Iberian' and the 'Brazilian' clones of MRSA.

OBJECTIVE: To determine the nature (clonal type and antibiotic resistance pattern) of methicillin-resistant Staphylococcus aureus (MRSA) strains recovered from the largest teaching hospital in Portugal and to detect temporal trends in clonal types during three consecutive surveillance periods in 1992--93, 1994--95 and 1996. METHODS: MRSA strains were characterized by chromosomal SmaI macrorestriction patterns using pulsed-field gel electrophoresis (PFGE) and by DNA fingerprints---applied to ClaI digests---capable of probing two specific areas of the staphylococcal chromosome: (1) the vicinity of the mecA gene, and (2) the attachment site(s) and copy number of transposon Tn554. The combination of these methods can generate 'clonal types' useful for epidemiological tracking of MRSA strains. RESULTS: During the 1992--93 collection period, 65% of MRSA strains carried the mecA polymorph I, Tn554 pattern E and PFGE pattern A (I::E::A)---a clonal type that was used to define the 'Iberian MRSA', which is widely spread throughout southern Europe. The representation of this clone decreased to 42% in 1994--95 and to 20% in 1996. At the same time, a second multiresistant MRSA strain carrying mecA polymorph XI, Tn554 type B and PFGE pattern B (XI::B::B)---a clonal type characteristic of the so-called 'Brazilian MRSA'---increased from 5% in 1992--93 to 36% in 1994--95 and 29% in 1996. CONCLUSIONS: Throughout the four years of surveillance, the Iberian and Brazilian MRSA types and their single subtype variants together have been responsible for the overwhelming majority (close to 90%) of all MRSA infections in the largest teaching hospital of Portugal. The mechanism of epidemicity of these two multiresistant international MRSA clones remains to be elucidated.

Journal Article↗