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Persistence of 10-year old Exxon Valdez oil on Gulf of Alaska beaches: the importance of boulder-armoring.

Oil stranded as a result of the 1989 Exxon Valdez spill has persisted for >10 years at study sites on Gulf of Alaska shores distant from the spill's origin. These sites were contaminated by "oil mousse", which persists in these settings due to armoring of underlying sediments and their included oil beneath boulders. The boulder-armored beaches that we resampled in 1999 showed continued contamination by subsurface oil, despite their exposure to moderate to high wave energies. Significant declines in surface oil cover occurred at all study sites. In contrast, mousse has persisted under boulders in amounts similar to what was present in 1994 and probably in 1989. Especially striking is the general lack of weathering of this subsurface oil over the last decade. Oil at five of the six armored-beach sites 10 years after the spill is compositionally similar to 11-day old Exxon Valdez oil. Analysis of movements in the boulder-armor that covers the study beaches reveals that only minor shifts have occurred since 1994, suggesting that over the last five, and probably over the last 10 years, boulder-armors have remained largely unmoved at the study sites. These findings emphasize the importance of particular geomorphic parameters in determining stranded oil persistence. Surface armoring, combined with stranding of oil mousse, results in the unexpectedly lengthy persistence of only lightly to moderately weathered oil within otherwise high-energy wave environments.

Alaska↗

Accumulation of polycyclic aromatic hydrocarbons by Neocalanus copepods in Port Valdez, Alaska.

Sampling zooplankton is a useful strategy for observing trace hydrocarbon concentrations in water because samples represent an integrated average over a considerable effective sampling volume and are more representative of the sampled environment than discretely collected water samples. We demonstrate this method in Port Valdez, Alaska, an approximately 100 km(2) basin that receives about 0.5-2.4 kg of polynuclear aromatic hydrocarbons (PAH) per day. Total PAH (TPAH) concentrations (0.61-1.31 microg/g dry weight), composition, and spatial distributions in a lipid-rich copepod, Neocalanus were consistent with the discharge as the source of contamination. Although Neocalanus acquire PAH from water or suspended particulate matter, total PAH concentrations in these compartments were at or below method detection limits, demonstrating plankton can amplify trace concentrations to detectable levels useful for study.

Alaska↗

Gene expression in caged juvenile Coho Salmon (Oncorhynchys kisutch) exposed to the waters of Prince William Sound, Alaska.

The 1989 Exxon Valdez oil spill (EVOS) resulted in the release of 258,000 barrels of crude oil into the waters of Prince William Sound (PWS), Alaska. The current study, conducted in 2004, sought to use juvenile Coho salmon (Oncorhynchus kisutch) caged in situ to determine whether biomarker induction differed at sites where the adjacent shoreline contained buried residues from the 1989 oil spill compared to sites that were never oiled. Juvenile Coho salmon were caged at five sites; three oiled during the 1989 EVOS and two that were not oiled. Tissue samples were collected from organisms caged at each site as well as a control group housed onboard the research vessel. Analysis of CYP1A, superoxide dismutase (SOD), and glutathione peroxidase (GPO) gene expression was conducted using real time reverse transcriptase polymerase chain reaction (rtRT-PCR). Statistically significant levels of CYP1A expression were observed at some sites indicating increased hydrocarbon exposure. No patterns were observed regarding sites that were originally oiled or not oiled by the 1989 EVOS, indicating that sources of PAHs other than EVOS oil occur in PWS.

Alaska↗

Impact of heptavalent pneumococcal conjugate vaccine on invasive disease, antimicrobial resistance and colonization in Alaska Natives: progress towards elimination of a health disparity.

We evaluated invasive pneumococcal disease (IPD), antimicrobial resistance and nasopharyngeal colonization before and after introduction of pneumococcal conjugate vaccine (PCV7) in Alaska Natives (AN), a population with high IPD rates. We obtained IPD rates from population-based surveillance. Colonization was determined from annual surveys among rural AN of all ages and from urban children. After vaccine introduction, vaccine-type IPD rates declined by 91% among AN children <2 years, by 80% among non-Natives <2 years, and by 40% for adults of all races (P<0.001 each). IPD decreased for isolates resistant to penicillin, erythromycin and cotrimoxazole (P<0.001 each). Vaccine-type colonization decreased among rural and urban children <5 years and among rural adults (P<0.001 each). PCV7 vaccine has eliminated a longstanding disparity of vaccine-type IPD for AN children. Decreased vaccine-type colonization and IPD in adults demonstrate indirect vaccine effects.

Adolescent↗

Long-term investigation of benthic fauna and the influence of treated ballast water disposal in Port Valdez, Alaska.

Benthic faunal structure and hydrocarbon concentrations in sediments were examined annually for 10 years (1989-1998) in Port Valdez, Alaska, where a near-bottom permitted discharge of treated tanker ballast waters containing residual petroleum has occurred since 1977. Measured concentrations of hydrocarbons in sediments near the discharge showed detectable increases in concentration, but on only one occasion were these increases either to levels exceeding ecotoxicological thresholds (Effects-Range Low, ER-L) or associated with alterations in the benthic community. Changes in faunal structure indicating disturbance were observed at one station near the discharge from 1995 to 1997 and were apparent as increased numbers of opportunistic taxa and anomalous trends in abundance and diversity. Minimum-effect (ME) hypotheses provided evidence of negligible to small effects on benthic infauna from disposal of treated ballast water. ME hypotheses have the potential to assist both researchers and managers by providing a tool to relate scientific results to ecological importance and decision criteria.

Alaska↗

Mercury concentrations in the fur of steller sea lions and northern fur seals from Alaska.

We compared total mercury (THg) concentrations in the fur of northern fur seals (Callorhinus ursinus) from the depleted Pribilof Islands population with those of both declining and thriving populations of Steller sea lions (Eumetopias jubatus) from Prince William Sound (PWS) and Southeast Alaska (SEA), respectively. Relatively low wet weight concentrations (ranges) of THg were detected in the fur of Steller sea lion (SSL) pups (0.90-3.14 microg/g) and juveniles (0.56-6.75 microg/g) from both areas in 1998 and 2000 compared to northern fur seal (NFS) pups (3.15-8.14 microg/g) in 2000. The mean concentration +/- SD for SSLs sampled were 1.46 +/- 0.64 microg/g for pups (n = 22) and 2.74 +/- 2.89 microg/g for juveniles (n = 6). Analyses indicated higher THg concentrations from SSL pups from PWS compared to the SEA. Mean +/- SD. THg in the NFS pups was 4.90 +/- 1.42 microg/g (n = 34) and for post-partum dams was 7.84 +/- 1.78 microg/g (n = 12).

Alaska↗

Variations in benthic fauna underneath an effluent mixing zone at a marine oil terminal in Port Valdez, Alaska.

The distributions of hydrocarbons and infauna in sediments below a permitted mixing zone for the disposal of treated ballast waters in Port Valdez, Alaska were examined annually, 1999-2001. The associations of biological measures and the abundance of selected benthic organisms to total aromatic hydrocarbons (TARO) ranged up to large-sized effects, as compared against minimum-effect criteria (/r/> or =0.63). The apparent sensitivity of three polychaete worms to low levels of hydrocarbons makes them particularly useful as indicators of future changes in spatial distributions of hydrocarbons associated with discharged effluent. In 2001, sediment TARO concentrations decreased and the correlation values of TARO to biotic variables were generally less than in 1999 and 2000. Evidence supports a conclusion of a strong but spatially limited association of some fauna with accumulations of petroleum hydrocarbons in these sediments.

Alaska↗

Adjustment of benthic fauna following sediment disposal at a site with multiple stressors in Port Valdez, Alaska.

The shallow subtidal macrobenthos at Port Valdez, Alaska, was examined to assess faunal adjustment following disposal of dredged sediments over a three-year period. Prior to sediment disposal, the infauna consisted of a relatively species-rich assemblage dominated by sessile polychaetes and bivalves. Six months after disposal, virtually all taxa present prior to dredging and disposal were rare or absent with opportunistic taxa dominant. Surveys performed 1.5 years after sediment disposal indicated faunal adjustment was in progress; large, sessile polychaetes and bivalves were still present in low numbers after 2.5 years. At one station, increasing organic enrichment by fish-wastes from adjacent processing plants resulted in a shift to a highly disturbed benthic assemblage. The trends in the faunal assemblage suggest that environmental conditions were still in a state of flux 2.5 years after the dredging event.

Alaska↗

Heterogeneity of natural organic matter from the Chena River, Alaska.

Water samples were collected in July 2001 from the Chena River in central Alaska. The natural organic matter (NOM) was size fractionated into particulate (POM,>0.45 microm), colloidal (COM,1kDa-0.45 microm) and dissolved (DOM,<1k Da) organic matter fractions, using filtration and ultrafiltration. The size-fractionated organic matter was then analyzed for organic carbon (OC) and nitrogen (N), isotopic (delta13C and delta15N) and molecular composition, using continuous flow isotope ratio mass spectrometry and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). Results of phase partitioning showed that, on average, about 6% of OC and 16% of N occurred in the form of POM while 66% of OC and 57% of N occurred in the form of COM, and 28% of the OC and 27% of the N were in the DOM form. Organic matter in the river water was found to be highly heterogeneous in terms of chemical composition and isotopic signatures. The C/N ratio was as low as 16+/-1 in the POM (n=2) to as high as 48+/-1 in the COM (n=3) and 38+/-4 in the DOM (n=3), suggesting a diagenetically younger POM. Values of delta13C increased with decreasing size, varying from -29.59+/-0.45% in the POM to -27.47+/-0.06% in the COM to -16.93+/-0.08% in the DOM. In contrast, values of delta15N decreased with decreasing size, from 2.64% in POM to 1.64% in COM to 1.33% in DOM. These results, together with radiocarbon measurements, suggest a preferential decomposition of lighter C isotope (12C) and heavier N isotopic (15N) from POM to COM to DOM. Results of py-GC/MS showed that the percentage of polysaccharides decreased with decreasing size, further supporting a degradation pathway of NOM from POM to COM and DOM in Chena River waters. More studies are needed to examine the seasonal and spatial variations of size-fractionated organic matter.

Alaska↗

Baseline levels of Hsp 70, a stress protein and biomarker, in halibut from the Cook Inlet region of Alaska.

The levels of Hsp 70, a heat shock protein, was quantitatively determined in Pacific halibut, Hippoglossus stenolepis, from the Cook Inlet region in south central Alaska. A dot blot analysis using a monoclonal antibody for Hsp 70 was combined with a standard protein analysis to determine Hsp 70 levels in 26 samples from gills. The average Hsp 70 concentration was 4.6 micrograms/mg, with levels ranging from 2.2 to 14.5 micrograms/mg total protein. Mercury in gill tissue also was measured and, in the 26 samples, only three samples had concentrations of mercury (X = 0.10 mg/kg, range = 0.09-0.11) above the minimum detection level.

Alaska↗

Venous thromboembolism hospitalizations among American Indians and Alaska Natives.

Cardiovascular disease (CVD) has been reported to be on the increase in the American Indian/Alaska Native (AI/AN) population. The Indian Health Service (IHS) hospital discharge database was used to describe venous thromboembolism (VTE)-associated hospitalizations among patients receiving IHS-reported medical care in the United States from 1980 through 1996. The average overall VTE-associated hospitalization rate in the AI/AN population during 1980-1996 was 33.1 per 100,000, however, the rate significantly decreased from 38.4 per 100,000 AI/ANs in 1980-1982 to 33.2 in 1994-1996. The average age at hospitalization was 50.4 years, which was consistent during the 17-year period. The overall annual VTE hospitalization rate was higher for females than for males (38.0 versus 27.7 per 100,000). The female VTE hospitalization rates decreased significantly from 46.1 per 100,000 in 1980-1982 to 36.7 per 100,000 in 1994-1996 (risk ratio: RR=1.3; 95% confidence interval: CI=1.1-1.4), while the rates for males remained unchanged. The VTE hospitalization rates also varied by geographic region. The hospitalization rate was highest in the East region (52.2 per 100,000) and lowest in the Alaska region (16.1 per 100,000). These data indicate that the overall VTE rate for AI/ANs may not have only decreased, but appears lower than the reported rate for Caucasians.

Adolescent↗

HLA antigens, alleles and haplotypes among the Yup'ik Alaska natives: report of the ASHI Minority Workshops, Part II.

As part of the American Society for Histocompatibility and Immunogenetics coordinated studies among minority populations, human leukocyte antigen (HLA) alleles were defined for 460 volunteer Yup'ik Eskimos from the Yukon Kuskokwim delta region of southwestern Alaska. The study group included 252 adults with no other first-degree relatives and 48 informative nuclear families. Full Yupik ancestry through both maternal and paternal grandparents was claimed by 81.1% of participants. HLA-A, -B, -Cw, -DRB1, and -DQB1 alleles were determined by SBT, SSOP, reverse SSOP, and/or RSCA according to the protocols of five participating laboratories. Polymorphism was limited with 3-6 alleles comprising > 80% of the alleles observed at each locus. Homozygosity was high, particularly at the HLA-A and -DQB1 loci, with 36.6% and 44% of individuals having a single allele defined at these respective loci. HLA-A, -B, and -DRB1 alleles were in Hardy-Weinberg equilibrium, whereas HLA-Cw and -DQB1 alleles gave significant deviation (p = 0.002; 0.005). Significant linkage disequilibrium (p < or = 0.00001) was observed in all pairwise evaluations. A new Cw*0806 allele was observed in high linkage disequilibrium with B*4801(Delta = 0.099; Delta(rel) = 1.0). Three extended haplotypes were found to have frequencies > 5%, the most prevalent being A*2402; B*4801; DRB1*0401; DQB1*0301 (0.0933). Comparison of available class I data indicate that the Yup'ik share several common alleles with other Native American populations, including: A*2402, *0206, *6801; B*1501, *2705, *3501, *4002, *4801, *5101; and Cw*0202, *0304, *0401. Comparisons of class II data also confirm a close relationship of the Yup'ik to two other Eskimo populations, Siberian and East Greenland Eskimos. DRB1*0401 and *1101, which occur in high frequency among these Eskimo populations, but not in other Native Americans, were also prevalent among the Yup'ik, with respective frequencies of 0.232 and 0.107.

Alaska↗

Environmental contaminants in four eider species from Alaska and arctic Russia.

Population declines in four species of eider; common (Somateria mollissima), king (Somateria spectabilis), spectacled (Somateria fischeri) and Steller's (Polysticta stelleri), have raised concerns about exposure to contaminants. Livers and kidney tissues were collected from eiders in Alaska and Russia for organic and elemental analyses. Results showed that organochlorine and many elemental levels were below toxic thresholds; however, in many cases, cadmium, copper, lead and serenium appeared high relative to other waterfowl and may warrant concern. With the exception of lead, local anthropogenic sources for these elements are not known. Although adverse physiological responses have not been documented in eiders, these four elements cannot be ruled out as contaminants of potential concern for some eider species.

Alaska↗

The effect of racial misclassification on estimates of end-stage renal disease among American Indians and Alaska Natives in the Pacific Northwest, 1988 through 1990.

Although several recent studies have documented a high incidence of end-stage renal disease (ESRD) among American Indians (AI) and Alaska Natives (AN), racial misclassification of AI/AN as members of other races on disease registries has not been accounted for. We conducted a study to link the Northwest Renal Network (NRN) ESRD database, which includes persons known to have begun dialysis in Idaho, Oregon, and Washington, and the Indian Health Service (IHS) patient registration file, which includes AI/AN enrolled for health care provided or paid for by IHS in the same states, for the period January 1, 1988 to December 31, 1990. Among the 3,356 persons included on the NRN ESRD database, 68 (2.0%) were coded as AI/AN. Of the 68 listed as AI/AN, 54 (79.4%) were identified on the IHS patient registration file. In addition to the 68 cases coded on the NRN as AI/AN, we identified 12 cases that were definitively matched to the IHS patient registration file but not coded AI/AN on the NRN. The age-adjusted annual incidence of ESRD treatment initiation among AI/AN in Idaho, Oregon, and Washington was 267.5 per million according to original racial coding on the NRN, but it increased to 311.6 per million after correction for racial misclassification. Among IHS-registered AI/AN, the annual incidence increased from 394.1 per million to 474.3 per million. Racial misclassification of AI/AN in ESRD registries may result in underestimates of AI/AN ESRD incidence for the United States.

Adolescent↗

Health, place and childhood asthma in southwest Alaska.

Social science theories of health and place posit that individuals perceive a relationship between characteristics of the geographic location in which they reside and their health, well-being, and self-identity. A number of ethnographies of health and place have studied how urban and suburban populations impacted by industrial pollution or waste have come to perceive a link between rates of cancer and their unhealthy environment. There has been little study of the applicability of the health and place framework to community perceptions of long-term chronic illness. This paper examines the asthma perceptions of Yup'ik parents of asthmatic children using data from semi-structured ethnographic interviews conducted in five villages and one town of the Yukon-Kuskokwim delta of southwest Alaska. Informants cited local climatic features, large-scale changes of the last 30 years to the village built landscape, and ongoing conditions of substandard housing and sanitation as etiological factors associated with childhood asthma. Our study suggests the need for further research concerning lay perceptions of one aspect of the epidemilogic transition-the association between chronic illness and place, especially in rural communities undergoing dramatic developmental change.

Adolescent↗

Oxygen release kinetics from solid phase oxygen in Arctic Alaska.

Child's Pad is a gravel construction surface that was contaminated with petroleum during oil-field service operations in Deadhorse, Alaska. As part of a remedial action plan, a buffer strip of uncontaminated sandy gravel was placed along sections of the pad boundary. A magnesium peroxide formulation manufactured by Regenesis, and sold as Oxygen Release Compound (ORC), was placed in the buffer strips. The ORC was intended to supply oxygen to aerobic microorganisms capable of degrading petroleum. Studies were conducted in the laboratory to determine initial oxygen release kinetics from ORC in contact with barrier soil. Studies quantified the biotic and abiotic catalytic mechanisms for converting hydrogen peroxide (a possible MgO2 intermediate) and ORC to oxygen and water, the effects of temperature on oxygen release from ORC, and the effect of field exposure on ORC viability. Barrier soil exhibited sufficient catalytic activity to convert hydrogen peroxide to oxygen faster than the expected biological demand. The oxygen evolution rate (OER) from ORC was lower at 7 degrees C than 21 degrees C by more than two times. The ORC recovered from Child's Pad after less than 1 year retained nearly all of the original available oxygen, although physical bridging was evident.

Alaska↗

Population studies on three Native Alaska population groups using STR loci.

Allele distributions for 13 short tandem repeat (STR) loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO and D16S539 were determined for three Native Alaskan population groups-Athabaskans, Inupiats, and Yupiks. Although genetic diversity is lower in the Native Alaskan populations compared with Caucasians and Africans, almost all loci are highly polymorphic in all three Native Alaskan groups. There was little evidence for departures from Hardy-Weinberg expectations (HWE) in any of the populations. Only two examples of detectable departures from HWE out of 39 locus-population tests performed were observed based on the exact test (Yupik, D8S1179, P=0.030; Yupik, D5S818, P=0.016). The F(ST) estimate overall 13 STR loci is 0.0309 for the Native Alaskan populations. Based on a genetic distance measure, Athabaskans are more closely related to Apaches and Navajos (all of the same linguistic group) than the other Native Alaskan groups. The F(ST) estimate for Athabaskans, Apaches and Navajos is 0.0180 and for Inupiats and Yupiks is 0.0167. The allele frequency data can be used for estimating DNA profile frequencies for Native Americans residing in Alaska.

Alaska↗

Hospitalization for motor vehicle injuries among American Indians and Alaska Natives in Washington.

OBJECTIVE: Little is known about the epidemiology of hospitalization for motor vehicle injury among American Indians and Alaska Natives (AI/ANs) in the Pacific Northwest. Current secondary data sources are inadequate to track this significant health problem. The purpose of this study was to determine the rate of hospitalization for motor vehicle injury in this population through linkage of Indian Health Service (IHS) patient registration data to a statewide hospital discharge database. METHODS: To create the numerator, IHS patient registration data were linked to Washington State hospital discharge abstracts from 1990 to 1994 for motor vehicle injury (ICD-9 E-codes 810-819). The denominator for this population was derived from the total number of IHS enrollees in 1992. Comparative numerator and denominator data for all residents were derived from the discharge database and Washington State intercensal population estimates, respectively. RESULTS: AI/ANs experienced a nearly two-fold higher rate of motor vehicle injury hospitalization (N = 588) compared to all residents [Incidence Ratio (I.R.): 1.82; 95% C.I. 1.52-2.19]. The greatest disparity in incidence rates occurred among 25-34 year olds (I.R. 2.18; 95% C.I. 1.53-3.10) and 35-44 year olds (I.R. 2.18; 95% C.I. 1.36-3.47). In-hospital mortality, severity of injury and length of stay were not different between the 2 groups. Median charges for American Indian hospitalizations were $6188 and the IHS was payer in, at most, 24% of hospitalizations. CONCLUSIONS: AI/ANs are at higher risk of hospitalization for motor vehicle injuries but, compared to all residents of Washington, appear to have similar severity of injuries and outcomes. Motor vehicle injury hospitalization among AI/ANs incurs substantial health care costs.

Accidents, Traffic↗