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Effects of sarcoptic mange and its control with oil of Cedrus deodara, Pongamia glabra, Jatropha curcas and benzyl benzoate, both with and without ascorbic acid on growing sheep: assessment of weight gain, liver function, nutrient digestibility, wool production and meat quality.

The aim of this study was to evaluate the therapeutic efficacy of commonly used acaricidal drugs in India and also to assess the effect of ascorbic acid as adjunct therapy in 72 growing sheep with sarcoptic mange, aged 5-6 months and weighing 20.4-31.7 kg. Eight replicates of nine animals were formed based on sex, and day 0 body weight. Drugs were applied locally on the affected parts daily and recovery changes in skin lesions were observed at the time of every application. L-ascorbic acid was administered intramuscularly. Skin scrapings were collected daily from each group and examined for the presence of mites. Body weights were measured every 10th day from day 0 to 60. Nutrient digestiblity was evaluated by studying digestibility coefficients for dry matter, crude protein, ether extract, crude fibre, nitrogen free extract, total carbohydrates and nutrient balance (nitrogen, calcium and phosphorus) for a 30-day period. The liver function was evaluated by bromosulphophthalein (BSP) dye retention time. The animals were shorn on day 60 post-treatment (PT). Meat quality assesment was carried out by killing sheep at 60 days PT and estimating pH, water-holding capacity (WHC), tenderness, muscle colour, rib eye area and fat thickness. The lambs treated with oil of Jatropha curcas ascorbic acid had significantly (P < 0.05) greater mean daily body weight gains (63.29 g) than the infected untreated control (41.10 g). This was also higher than the mean daily weight gain in other treated groups. Infected untreated sheep showed significantly (P < 0.01) reduced digestibility coefficients for dry matter, crude protein, crude fibre, ether extract and total carbohydrate, but no significant differences for nitrogen-free extract. Treated sheep had significantly higher positive nitrogen, calcium and phosphorus balances compared with infested untreated sheep. Oil of J. curcas plus ascorbic acid (OJC-AA) treated group was better over all other treated groups with respect to nutrient digestibility. The BSP test revealed significant (P < 0.05) increase in BSP retention time in sheep with sarcoptic mange. Post -treatment, the BSP retention time decreased in all treated groups and the decrease was maximum in OJC-AA treated group. The carcasses of sheep treated with OJC-AA had significantly (P < 0.01) higher water holding capacity, rib eye area and back fat thickness than the untreated infected control group. The muscle pH and tenderness values were significantly lower in OJC-AA treated group post-slaughter than infested untreated control group. Muscle colour of OJC-AA treated group was maximum bright red. The lambs treated with OJC-AA had significantly (P < 0.05) greater clean fleece weight and fleece yield than the untreated infected group. It is concluded that OJC was the better therapy for sarcoptic mange of sheep and ascorbic acid as adjunct therapy is advisable. OJC-AA therapy may be better from the point of view of improving two most important production parameters in sheep, that is, wool yield and meat production.

Animals↗

Cost-benefit evaluation of artificial insemination for genetic improvement of wool-producing sheep.

The financial costs and benefits associated with the use of artificial insemination (AI) in commercial flocks are evaluated. Benefits are calculated in terms of net present values after summing the discounted value of benefits over 20 years. Two breeding strategies are evaluated. With the first, AI is used to produce flock ewes and wethers. The method is unlikely to be profitable unless high breeding value rams are available for AI programs with fresh semen. With the second, AI is used to produce home-bred rams, which in turn sire flock ewes and wethers. This approach is more likely to be profitable. The cost of AI per lamb weaned from laparoscopic AI programs is about $100. Benefits exceed this cost for rams of very high merit when wool prices are moderate or higher. Flock structure has a significant effect on the benefits. Flocks with low wether retention rates have benefits half that of flocks that retain most wethers to 6 years of age. AI with purchased semen also provides benefits to risk management for owners of commercial flocks who wish to breed their own replacement flock rams.

Animals↗

Rainfall and breed effects on the efficacy of ivermectin jetting fluid for the prevention of fly strike and treatment of infestations of lice in long-wooled sheep.

An ivermectin-based jetting fluid was assessed for its efficacy in the prevention of blowfly strike and treatment of lice in long-wooled sheep. The ivermectin concentrate was diluted to 0.03 mg/mL and applied via a standard hand jetting technique. In insectary studies, using laboratory-reared Lucilia cuprina, sheep jetted with ivermectin were protected from induced breech and body strikes for 18 weeks after treatment. Merino and Corriedale sheep were equally protected, as were sheep subjected to 25 mm simulated rainfall at 1, 3 or 6 hours after jetting. Naturally occurring infestations of lice, Bovicola (Damalinia) ovis, were successfully treated with ivermectin jetting fluid in Merino and Corriedale sheep using a standard hand jetting technique. In addition, the efficacy of treatment was not affected by simulated rainfall at 1, 3 or 6 hours after treatment.

Administration, Topical↗

Byssinosis in carpet weavers exposed to wool contaminated with endotoxin.

All the 303 full time day workers in a carpet weaving factory were submitted to a physical examination, chest radiography, and vitalograph test, and answered a respiratory questionnaire. Fifty four healthy non-exposed subjects served as controls. Dust concentrations and concentrations of bacterial endotoxin were measured. Of the 303 workers, 259 (85.5%) had airway symptoms and 62 (20.5%) had maximum mid-expiratory flow (MMF) values of less than 60% compared with 9.2% of the controls. The symptoms in 68 workers (22%) were compatible with byssinosis and 36 of these workers underwent vitalography before starting work and after four hours work on Mondays when significant reductions of their FEV1 and MMF were found. Twenty one of these 36 workers were tested on Tuesday and no differences in these measurements were found between measurements before work started and four hours later. The airborne dust concentrations in the factory were high and bacterial endotoxin was found. These findings suggest that a large number of workers in this carpet weaving factory suffer from a disease indistinguishable from byssinosis even though wool is used almost exclusively, the only cotton being the warp. The finding of endotoxin together with the absence of cotton confirms the theory that "byssinosis" is due to bacterial endotoxin rather than to cotton per se.

Adult↗

Detection, pathogenesis, and prevention of damage to human granulocytes caused by interaction with nylon wool fiber. Implications for filtration leukapheresis.

Granulocytes collected by reversible adhesion to nylon wool fiber (NWF) function relatively well in standard in vitro tests; however, they have an abnormally shortened survival time in the circulation. Assuming that this rapid disappearance represents clearance and that recognition by phagocytes is important for such clearance, we used an autologous in vitro cell:cell recognition assay to determine whether phagocytes can detect cellular changes induced by exposure of normal granulocytes to NWF. Human granulocytes incubated with NWF 1 h at 37 degrees C, eluted with 20% acid citrate dextrose plasma, and washed stimulated the hexose monophosphate shunt activity of normal granulocytes an average of twofold (193+/-40% of controls), indicating a recognition response. NWF-induced granulocyte recognition was not dependent on plasma factors or activated complement components but was dependent on the time that the granulocyte was on the NWF and was maximal by 60 min of exposure. After elution from NWF, granulocytes demonstrated resting glucose oxidation rates only slightly higher than normal; however, during the first 20 min of exposure to NWF, granulocytes increased their rate of (14)CO(2) production from [1-(14)C]glucose three- to five-fold. Therefore, experiments were performed to determine whether toxic oxygen metabolites produced by NWF-adherent cells might contribute to recognition. The results showed that (a) normal granulocytes exposed to NWF in the presence of scavengers of superoxide anion (superoxide dismutase) or free radicals (ascorbate, mannitol, or benzoate) and washed before assay did not stimulate glucose oxidation of indicator granulocytes; and (b) NWF granulocytes prepared from cells unable to generate high levels of toxic oxygen metabolites, i.e. cells prepared anaerobically or from a patient with chronic granulomatous disease, also failed to stimulate indicator granulocytes. Human granulocytes placed in contact with NWF show an oxidative burst and become recognizable to other phagocytes. Free radical scavengers are effective in minimizing this recognition conferred on NWF-procured granulocytes.

Animals↗

Effect of some process parameters in enzymatic dyeing of wool.

This article reports on the dyeing of wool using an enzymatic system comprising laccase; dye precursor, 2,5-diaminobenzenesulfonic acid; and dye modifiers, catechol and resorcinol. Enzymatic dyeing was performed as a batchwise process at the temperature and pH of maximum enzyme activity. The effects of the process variables reaction time, enzyme, and modifier concentration on fabric color were studied, according to an appropriate experimental design. Different hues and depths of shades could be achieved by varying the concentration of the modifiers and the time of laccase treatment. The duration of the enzymatic reaction appeared to be the most important factor in the dyeing process. Thus, the dyeing process, performed at low temperature and mild pH, was advantageous in terms of reduced enzyme and chemical dosage.

Analysis of Variance↗

Treatment of ewes at different stages of pregnancy with epidermal growth factor: effects on wool growth and plasma concentrations of growth hormone, prolactin, placental lactogen and thyroxine and on foetal development.

Pregnant ewes at 57, 75 and greater than or equal to 101 days of gestation were infused with approximately 3 mg of epidermal growth factor (EGF) for a period of 24-28 h. The strength of attachment of the wool fibres to the skin became progressively reduced during the week following treatment. After 3 weeks, the mean forces required to pluck staples had declined to between 10 and 30% of the pre-infusion measurements and the fleeces were able to be removed easily by hand. Increases in growth hormone and placental lactogen occurred in plasma of ewes during EGF treatment. Prolactin was not affected by EGF but circulating levels of thyroxine declined. The concentrations of these hormones returned to approximate pretreatment values within 24 h of the end of infusion. EGF treatment at the dose levels used did not appear to significantly affect the development or viability of the foetus.

Animals↗

Simulated effects of reproductive performance on life-cycle efficiency of lamb and wool production at three lambing intervals.

A deterministic computer model of a purebreeding sheep production system was used to simulate effects on life-cycle efficiency from genetic changes in lambing rate (LB), fertility (FERT), precocity of fertility (PREC), and estrus season length (ES) under annual (Y), 4-mo (A), and 73-d (S) breeding intervals. Ewes and lambs of a 1,000-ewe flock were fed to requirement through five yr, beginning with ewe lambs at the optimal breeding season and accumulating feed input (TDN) and market lamb equivalent weight of output (EBW), including cull ewes and wool. For annual lambing, improvement in cost (TDN/EBW equivalence) per 10% increase in genetic potential was -9.8, -8.2, -1.3, and -.8% for LB, FERT, PREC, and ES, respectively. For the A and S lambing intervals, corresponding gains in TDN efficiency were -19 for FERT and -9% for LB when ES was 72 d, but only -6 and -5% when ES was 192 d. Increasing PREC by 10% improved cost -3% under accelerated systems when ES was 72 d, but less than -1% when ES was 192 d. Cost reduction from 10% longer ES under A and S breeding intervals was greater when other reproductive performance was low (-11 and -17%) rather than high (-2 and -3%). Reduction in costs for A and S vs Y lambing intervals was greatest with long ES and was greater with low (-43 and -53%) than with high reproductive performance (-20 and -24%). When ES was 72 d, A and S intervals would reduce costs for high (-9 and -11%) but increase costs for low reproductive traits. Corresponding increases in CP/TDN required in diet would reduce gains in TDN/EBW only slightly. These results should aid in development of optimum selection criteria for stocks used for pure or rotation crossbreeding or as maternal parents of terminal crosses.

Animal Husbandry↗

Effects of inbreeding on reproduction and wool production of Rambouillet, Targhee, and Columbia ewes.

Because of a traditional interest in inbreeding as a tool for breed improvement, it was desirable to evaluate the effects of inbreeding on the most important products of the sheep industry, lamb and wool production. The data for this study were based on 13,807 ewe and 16,470 lamb records from Rambouillet (R), Targhee (T), and Columbia (C) sheep collected over 9 or 10 yr from 54 inbred lines. Average inbreeding of the lambs and ewes involved was near 25 and 20%, respectively. The combined effects of lamb's and dam's inbreeding reduced litter weight weaned at 120 d approximately .5 kg for each percentage of increase in inbreeding in every breed. For ewes and lambs of average inbreeding, the reductions relative to noninbred ewes amounted to 12.4, 10.3, and 11.3 kg for R, T, and C, respectively. The reductions constituted declines in weight weaned per ewe of 34, 25, and 28%. Similarly, net reproduction rate (lambs weaned per ewes put into breeding) was reduced more than one percentage point for each percentage of increase in inbreeding, with net declines at average inbreeding of 31.7, 23.5, and 25.7 percentage points for each breed, respectively. These reductions were equivalent to reductions in net rate of 29, 20, and 23%. The combined effect of lamb and dam inbreeding reduced weaning weight by 3.5, 2.6, and 2.2 kg, which constituted reductions of 10, 7, and 6%, respectively. Reduced weaning weight was approximately 30% as important as reduced net reproduction rate in contributing to the decline in litter weight weaned. Effects on fleece weight were curvilinear and amounted to reductions of .35, .18, and .00 kg, respectively, at levels of average inbreeding; however, Columbia fleece weights declined rapidly at levels exceeding 20% for ewes. Potential selection differentials were reduced 16% at inbreeding levels of 25 and 20% for lambs and dams, respectively, and 62% at levels of 55 and 50%. The average economic loss per ewe in value of production was estimated at $17 for average inbreeding and as high as $36 for inbreeding approaching 50%. It seems that the use of inbreeding as a tool for improving productive merit in sheep is much more certain to be a detriment, economically and genetically, than an advantage.

Animals↗

Estimation of genetic parameters for wool fiber diameter measures.

Genetic and phenotypic correlations and heritability estimates of side, britch, and core diameters; side and britch CV; side and britch diameter difference; and clean fleece weight were investigated using 385 western white-faced ewes produced by 50 sires and maintained at two locations on a selection study. Data were analyzed using analysis of variance procedures, and effects in the final model included breed of sire-selection line combination, sire within breed-selection line, and location. Heritabilities were estimated by paternal half-sib analysis. Sires within breed-selection line represented a significant source of variation for all traits studied. Location had a significant effect on side diameter, side and britch diameter difference, and clean fleece weight. Age of ewe only affected clean fleece weight. Phenotypic and genetic correlations among side, britch, and core diameter measures were high and positive. Phenotypic correlations ranged from .68 to .75 and genetic correlations ranged from .74 to .89. The genetic correlations between side and britch diameter difference and side diameter or core diameter were small (-.16 and .28, respectively). However, there was a stronger genetic correlation between side and britch diameter difference and britch diameter (.55). Heritability of the difference between side and britch diameter was high (.46 +/- .16) and similar to heritability estimates reported for other wool traits. Results of this study indicate that relatively rapid genetic progress through selection for fiber diameter should be possible. In addition, increased uniformity in fiber diameter should be possible through selection for either side and britch diameter difference or side or britch CV.

Age Factors↗

Performance of hair breeds and prolific wool breeds of sheep in southern Illinois: effect of breed of service sire on lamb production of Suffolk and Targhee ewes.

The effects of year, age of ewe, sex of lamb, breed of ewe, and breed of service ram on fertility, prolificacy, lamb survival, lamb weaning weight, and ewe productivity of Suffolk and Targhee ewes mated to Finnsheep, Combo-6, Booroola Merino, St. Croix, and Barbados rams were estimated. Matings were in four consecutive years using three rams per breed of service ram each year, with a total of 758 ewe-year observations. There was a significant effect of year on fertility, prolificacy, and ewe productivity. Two- and three-year-old ewes had lowest (P < .05) fertility rates, whereas 3-, 4-, and 5(+)-yr-old ewes had higher (P < .01) prolificacy and ewe productivity than 2-yr-old ewes. Ram lambs were .9 kg heavier (P < .01) at weaning than were ewe lambs. Suffolk ewes gave birth to .13 more (P < .05) lambs per ewe lambing, and their lambs were 1.4 kg heavier (P < .01) than lambs from Targhee ewes. Ewes mated to St. Croix and Barbados rams had higher (P < .10) fertility rates than ewes mated to Finnsheep rams. Lambs sired by Barbados, Finnsheep, and St. Croix rams had higher (P < .05) survival rates to weaning than lambs sired by Booroola Merino rams. The heaviest (P < .01) lambs at weaning were those sired by Finnsheep and Combo-6 rams. Ewes mated to hair-breed rams had greater (P < .05) performance for the fitness traits of fertility and lamb survival but lower (P < .01) lamb weaning weights than ewes mated to wool-breed rams.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗

Performance of hair breeds and prolific wool breeds of sheep in southern Illinois: lamb production of F1 ewe lambs.

The effects of year, sex of lamb, breed of dam, and breed of sire were estimated for age at puberty, breeding to lambing interval, breeding weight, ovulation rate, and ewe productivity (weight of lamb weaned per ewe exposed) and its component traits in F1 ewe lambs produced from Suffolk and Targhee ewes mated to rams of three wool breeds (Finnsheep, Combo-6, and Booroole Merino) and two hair breeds (St. Croix and Barbados). Ewe lambs were pasture-mated to Dorset rams when they were 8 to 10 mo of age. A total of 334 F1 ewe lambs, born in the late winters of 1986 through 1989, were exposed Year of birth was an important source of variation for age at puberty (P < .05), breding weight (P < .01), fertility (P < .05), prolificacy (P < .10), lamb survival (P < .10), lamb weaning weight (P < .05), and ewe productivity (P < .01). Ram lambs born to the F1 ewe lambs had lower (P < .10) survival rates to weaning but were heavier (P < .01) at weaning than contemporary ewe lambs. The F1 ewe lambs from Suffolk dams reached puberty earlier (P < .01), were heavier (P < .01) at breeding, and had higher (P < .01) ovulation rates and greater (P < .01) prolificacy than ewe lambs from Targhee dams.(ABSTRACT TRUNCATED AT 250 WORDS)

Adaptation, Physiological↗

Effects of fish meal and sodium bentonite on daily gain, wool growth, carcass characteristics, and ruminal and blood characteristics of lambs fed concentrate diets.

We evaluated the effects of replacing some soybean meal (SBM) protein with fish meal (FM) protein in diets adequate and slightly deficient in CP, with or without .75% sodium bentonite (NaB) on performance and ruminal and blood metabolites of individually fed Suffolk lambs. Diets were based on corn, SBM, and cottonseed hulls. In Exp. 1, five lambs were assigned to each of the three dietary treatments (11% CP with 3% FM, 13% CP with 0 or 3% FM). Lambs fed diets that contained 11% CP with 3% FM or 13% CP with 0% FM had similar DMI and ADG. Gain and feed efficiency were slightly improved (P = .18) by the 13% CP diet with 3% FM. In Exp. 2, 32 lambs were assigned to four dietary treatments (13.5% CP of DM) in a 2 x 2 factorial arrangement (0 or 3% FM, and 0 or .75% NaB on an as-fed basis). The DMI and ADG were increased (P < .05) by FM and NaB supplementation. Interactions (P < .05) revealed that NaB increased DMI, ADG, gain per feed (g/kg of DMI), and plasma urea N concentration in the absence of FM but not in the presence of FM in the diet. Neither FM nor NaB influenced (P = .25) wool growth. Total ruminal VFA were increased (P < .06) by FM and NaB. Differences in mineral content of phalanx bone, liver, and kidney were small and may be related to the mineral content of diets and the effect of NaB on mineral solubilities. Similar DMI and ADG of lambs fed FM and NaB separately and in combination suggest that their beneficial effect is not additive.

Animal Feed↗

Genotypic expression at different ages: II. Wool traits of sheep.

Genetic parameters for wool traits for Columbia, Polypay, Rambouillet, and Targhee breeds of sheep were estimated with single- and multiple-trait analyses using REML with animal models. Traits considered were fleece grade, fleece weight, and staple length. Total number of observations ranged from 11,673 to 34,746 for fleece grade and fleece weight and from 3,500 to 11,641 for staple length for the four breeds. For single-trait analyses, data were divided by age of ewe: young ages (age of 1 yr), middle ages (ages of 2 and 3 yr), and older ages (age greater than 3 yr). Heritability estimates averaged over breeds for fleece grade decreased from .42 at a young age to .37 for older ages. For fleece weight, heritability estimates averaged .52, .57, and .55 within the successively older groups. Heritability estimates for staple length averaged .54 for young and middle age classes. Few older ewes had staple length measurements. After single-trait analyses, new data sets were created for three-trait analyses with traits defined by three age classes when animals were measured. Heritability estimates with three-trait analyses, except for a few cases, were somewhat greater than those from single-trait analyses. For fleece grade, the genetic correlations averaged over breeds were .72 for young with middle, .42 for young with older, and .86 for middle with older age classes. For fleece weight, the average genetic correlations were .81, .83, and .98. For staple length, the average genetic correlation for young with middle age classes was .82. Estimates of genetic correlations across ages varied considerably among breeds. The average estimates of correlations suggest that fleece grade may need to be defined by age, especially for the Columbia and Rambouillet breeds. For fleece weight and staple length, however, the average correlations suggest no need to define those traits by age.

Aging↗

Relationships between hemoglobin type and reproduction, lamb, wool and milk production and health-related traits in crossbred ewes.

Relationships among hemoglobin (Hb) types and production traits were examined in 294 crossbred ewes from North Country Cheviot, Dorset, Finnsheep and Romney rams, and Suffolk and Columbia-type ewes. Hb BB ewe lambs were youngest at first estrus, while Hb AA ewe lambs were oldest. Ewe lamb fertility was similar for Hb AB and Hb BB ewes, and both were superior to Hb AA ewes. When Finnsheep crossbreds were excluded, from which Hb BB was absent, Hb BB ewes had higher ewe lamb fertility than did Hb AB ewes. There was a minor advantage of the A over the B allele in ewe lamb prolificacy. Hb BB ewes were highest, Hb AB ewes were intermediate and Hb AA ewes were lowest for average fertility, prolificacy per ewe lambing, lambs born per ewe exposed to mating, total number of lambs weaned and total kilograms of lamb weaned. Grease wool production, staple length, fiber diameter grade, incidence of medullated fibers and incidence of cotted fleeces all were similar across Hb types. Likewise, Hb type did not affect milk production or composition. Ewes with Hb AB had the lowest incidence of footrot; Hb AA and Hb BB ewes were similar. Ewes with Hb AA had the lowest fecal parasite egg counts, while ewes with Hb AB or Hb BB were similar. Ewes with Hb AA also had the lowest incidence of mastitis, Hb BB ewes were intermediate and Hb AB ewes were highest. The combination of overdominance (as for footrot resistance), beneficial effects of the A allele on health-related traits (as for mastitis and parasite resistance) and the beneficial effect of the B allele on reproductive traits, if real, could partly explain the genetic polymorphism commonly reported at the Hb locus in sheep.

Animals↗

Collaborative study of the glass wool filtration method for the recovery of virus inoculated into ground beef.

A method for estimating viral population levels in ground beef was studied collaboratively in 7 laboratories. The collaborators recovered virus from 6 inoculated samples. Three samples were replicates of the high virus concentration 050 plaque-forming units (pfu)/g) and 3 replicates represented the low concentration (10 pfu/g). Six of the 7 collaborators recovered acceptable levels of virus from the samples. The per cent of variation was 30.6 for the high concentration and 18.5 for the low concentration. Collaborators did not differ from one another significantly in the results obtained for the 10 pfu/g samples, but results from one collaborator were significantly low for the recovery of virus from the 50 pfu/g samples. The results indicate that the glass wool filtration method is adequate for the detection of a number of viruses that may be found in foods. The method has been adopted as official first action.

Animals↗

Keratinolytic fungi in Egyptian soils. I. Baited with hair and wool.

150 soil samples were collected, 90 from Nile Valley and Delta, 36 from desert and 24 from salt marshes. Human, buffalo and cow hair and sheep wool were used as baits at three incubation temperatures. Forty-four species which belong to twenty-one genera at 27 degrees C and forty-two which belong to twenty-two genera at 37 degrees C were collected. We isolated the following keratinophilic fungi Chrysosporium tropicum, C. keratinophilum, C. indicum, C. pannicola, C. queenslandicum, Trichophyton terrestre, T. mentagrophytes and Microsporum gypseum. Several other saprophytic fungi were isolated. No fungi were isolated at 45 degrees C.

Animals↗

[Electrophoretic studies of acid mucopolysaccharides in skin, prekeratin of hair follicles and in keratoses of wool].

Mucopolysaccharide complex isolated from skin, prekeratin of hair follicles and keratoses of wool by paper electrophoresis was separated into chondroitin sulphate and hyaluronic acid. The content of chondroitin sulphate in all the objects under investigation except for gamma-keratosis is higher than that of hyaluronic acid. The highest level of chondroitin-sulphuric acid is observed in the matrix of prekeratin of hair follicles and cuticule of wood fibre, i. e. beta-keratosis.

Animals↗