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At least 451 records · Page 25Linked to original sources
PubMed1989

Basic fibroblast growth factor, albumin, and transferrin purified from rat rhodamine fibrosarcoma tissue are all essential for growth of primary tumor cells from the same tissue in serum-free medium.

Primary Rhodamine fibrosarcoma (RdF) cells from rats were shown to grow in serum-free medium supplemented with basic fibroblast growth factor (bFGF), albumin, and transferrin, all of which were purified from RdF tissue. Their growth rate with these supplements was similar to that of cells in medium supplemented with calf serum. bFGF purified from RdF tissue (Rd-bFGF), which was previously designated as DNA synthesis factor, stimulated the growth of primary RdF cells maximally at 30 ng/ml in the presence of the other two proteins. Albumin and transferrin were separated from partially purified tumor growth stimulating activity which was previously shown to stimulate growth of primary RdF cells in serum-free medium. The albumin (RdA) and transferrin (RdT) found in the extract of RdF tissue were not due simply to contamination of the tissue with blood, but to their accumulations in the tissue. The growth stimulatory activities of RdA and RdT on primary RdF cells in serum-free medium were maximal at 30 and 10 micrograms/ml, respectively. These results suggest that Rd-bFGF, RdA, and RdT, all of which accumulate in the tumor tissue, are essential for growth of RdF cells in the tissue.

Albumins↗
PubMed1993

A new method for the detection of viable cells in tissue sections using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT): an application in the assessment of tissue damage by surgical instruments.

This report describes a new method using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to visualize live viable tissue at the microscopic level. The visualization of the MTT stained tissue provides a metabolic assessment of tissue damage, and can be utilized as an extension of conventional hematoxylin-eosin (H & E) staining. In this report, several tissues were studied with MTT and H & E staining after incisions had been made by a variety of surgical dissecting instruments. A marked improvement in the detection of tissue damage was seen using MTT, regardless of how the damage was caused, i.e., physical, heat, or photon energy. In addition, a distinct zone of damage not noted on conventionally prepared and stained tissues is readily apparent. Thus MTT staining will have utility in both clinical and research studies, concerned with assessing the viability of tissues.

Animals↗
PubMed1988

Characterization of anti-tissue factor antibody and its use in immunoaffinity purification of human tissue factor.

Human brain tissue factor apoprotein was recently purified to homogeneity in this laboratory by affinity chromatography utilizing factor VII bound to immobilized anti-factor VII (Anal Biochem 165, 365-370, 1987). A potent polyclonal anti-tissue factor antibody has now been raised to the purified apoprotein. Immunostaining of purified tissue factor with this polyclonal IgG yielded a single major band with an apparent molecular weight of 47,000, which corresponds to the mobility on SDS-PAGE of tissue factor apoprotein. Immunostaining of a crude Triton extract of brain tissue yielded two additional bands, with apparent molecular weights of 54,000 and 40,000 Da. An anti-tissue factor IgG was coupled to Affi-Gel-15 to prepare an immunoadsorbant column. The two additional proteins in the crude Triton extract recognized by Western blotting did not bind to the column. This permitted its use to develop a simple, efficient technique for purification of human tissue factor apoprotein.

Antibodies↗
PubMed2006

The use of parental data to evaluate soft tissues in an Anatolian Turkish population according to Holdaway soft tissue norms.

INTRODUCTION: The relative influence of genetic and environmental factors in the etiology of malocclusion has long been a matter for discussion. The aim of this study was to compare the soft tissue structures of parents and their prepubertal, pubertal, and postpubertal offspring according to the Holdaway soft tissue norms to determine the similarity among them. Differences related to age and sex between the parents and offspring were also evaluated. METHODS: Lateral cephalometric radiographs were used to determine the similarities in soft tissue structures in 120 families, which were divided into prepubertal (n = 40), pubertal (n = 40), and postpubertal (n = 40) groups according to the children's skeletal ages. A total of 12 variables (2 angular and 10 linear) were measured according to Holdaway's soft tissue analysis. Pairs were formed between family members (mother to son, mother to daughter, father to son, father to daughter, midparent (mean of the father's and mother's values) to son, and midparent to daughter) in each group. Descriptive statistics and multiple linear regression analysis were performed. RESULTS: Statistically significant correlations between parents and their offspring were found. The parental data showed that a mother's genetic influence on her child's soft tissues was greater than the father's. Moreover, it was found that daughters had greater genetic influences from their parents than did sons. As age increased, the similarity was greater. Soft tissue facial angle (beta: 0.671), soft tissue chin thickness (beta: 0.549), and basic upper lip thickness (beta: 0.537) were the most similar variables between parents and their offspring. CONCLUSIONS: A few selected cephalometric measurements of the parents might furnish important data to the clinician in predicting a child's facial growth characteristics and aging process.

Adolescent↗
PubMed2005

Tissue specific induction of cytochrome P450 (CYP) 1A1 and 1B1 in rat liver and lung following in vitro (tissue slice) and in vivo exposure to benzo(a)pyrene.

Cytochrome P-450s (CYPs) detoxify a wide variety of xenobiotics and environmental contaminants, but can also bioactivate carcinogenic polycyclic aromatic hydrocarbons, such as benzo(a)pyrene (BaP), to DNA-reactive species. The primary CYPs involved in the metabolism and bioactivation of BaP are CYP1A1 and CYP1B1. Furthermore, BaP can induce expression of CYP1A1 and CYP1B1 via the aryl hydrocarbon receptor. Induction of CYP1A1 and CYP1B1 by BaP in target (lung) and non-target (liver) tissues was investigated utilizing precision-cut rat liver and lung slices exposed to BaP in vitro. Tissue slices were also prepared from rats pretreated in vivo with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to induce expression of CYP1A1 and CYP1B1. In addition, in vivo exposure studies were performed with BaP to characterize and validate the use of the in vitro tissue slice model. In vitro exposure of liver and lung slices to BaP resulted in a concentration-dependent increase in CYP1A1 and CYP1B1 mRNA and protein levels, which correlated directly with the exposure-related increase in BaP-DNA adduct levels observed previously in the tissue slices [Harrigan, J.A., Vezina, C.M., McGarrigle, B.P., Ersing, N., Box, H.C., Maccubbin, A.E., Olson, J.R., 2004. DNA adduct formation in precision-cut rat liver and lung slices exposed to benzo(a)pyrene. Toxicological Sciences 77, 307-314]. Pretreatment of animals in vivo with TCDD produced a marked induction of CYP1A1 and CYP1B1 expression in the tissue slices, which was similar to the levels of CYP1A1 and CYP1B1 mRNA achieved in liver and lung following in vivo treatment with BaP. Following in vitro exposure to BaP, the levels of CYP1A1 were greater in the lung than the liver, while following all exposures (in vitro and in vivo), the levels of CYP1B1 mRNA were greater in lung tissue compared to liver. The higher expression of CYP1A1 and CYP1B1 in the lung was associated with higher levels of BaP-DNA adducts in the lung slices (Harrigan et al.'s work) and together, these results may contribute to the tissue specificity of BaP-mediated carcinogenesis.

Animals↗
PubMed2002

Doppler ultrasound detection of shear waves remotely induced in tissue phantoms and tissue in vitro.

In shear wave elasticity imaging (SWEI), mechanical excitation within the tissue is remotely generated using radiation force of focused ultrasound. The induced shear strain is subsequently detected to estimate visco-elastic properties of tissue and thus aid diagnostics. In this paper, the mechanical response of tissue to radiation force was detected using a modified ultrasound Doppler technique. The experiments were performed on tissue mimicking and tissue containing phantoms using a commercial diagnostic scanner. This scanner was modified to control both the pushing and probing beams. The pushing beam was fired repetitively along a single direction while interlaced probing beams swept the surrounding region of interest to detect the induced motion. The detectability of inhomogeneous inclusions using ultrasonic Doppler SWEI method has been demonstrated in this study. The displacement fields measured in elastic phantoms clearly reveal the oscillatory nature of the mechanical relaxation processes in response to impulsive load due to the boundary effects. This relaxation dynamics was also present in cooked muscle tissue, but was not detected in more viscous and less elastic phantom and raw muscles. Presence of a local heterogeneity in the vicinity of the focal region of the pushing beam results in generation of a standing wave field pattern which is manifested in the oscillatory response of the excited region of the tissue. There has been made an assumption that dynamic characteristics of the relaxation process may be used for visualization of inhomogeneities.

Animals↗
PubMed1998

A peptide sequence from mouse tissue factor inhibits human tissue factor dependent factor X activation.

Synthetic peptides based on the putative factor X recognition site of human (Thr-Leu-Tyr-Tyr-Trp-Lys-Ser-Ser-Ser-Ser), rabbit (Thr-Leu-Tyr-Tyr-Trp-Arg-Ala-Ser-Ser-Thr), and murine tissue factor (Ile-Ile-Thr-Tyr-Arg-Lys-Gly-Ser-Ser-Thr) were dose-dependent inhibitors of human tissue factor/factor VIIa catalyzed factor X activation with IC50 values of 220, 17, and 33 microM, respectively. The mouse results were highly surprising given the low homology between the human and mouse sequence (40%) and that mouse tissue factor, in contrast with rabbit tissue factor, does not support the procoagulant activity of human factor VIIa on factor X. The inhibitory mechanism of the murine peptide was noncompetitive with respect to factor X but competitive with respect to tissue factor, indicating the peptide competes with tissue factor (or the tissue factor/factor VIIa complex) for binding to factor X. The peptide could be N-terminally truncated by two Ile without loss of inhibitory activity or changed inhibitory mechanism. Substitution of two Gly for the two Ile, which increased solubility, decreased IC50 to 17 microM whereas scrambling the peptide made it inactive.

Amino Acid Sequence↗
PubMed1999

Plasma tissue factor pathway inhibitor and tissue factor antigen levels after administration of heparin in patients with angina pectoris.

The hypercoagulability is associated with expression of tissue factor in patients with angina. Tissue factor pathway inhibitor regulates the extrinsic coagulation pathway mediated by tissue factor. Plasma samples were obtained from 14 patients with angina pectoris and 9 with chest pain syndrome before and 5, 30, 60, and 120 minutes after administration of heparin (50 IU/kg). The tissue factor and prothrombin fragment 1+2 levels before administration were elevated in patients with angina pectoris and were reduced to the levels of chest pain syndrome after the administration. The free tissue factor pathway inhibitor levels after the administration were higher in patients with angina pectoris than in patients with chest pain syndrome. Plasma tissue factor pathway inhibitor levels correlated positively with plasma tissue factor and prothrombin fragment 1+2 levels. We showed that plasma-free TFPI levels after administration of heparin, which may indicate endothelial cell associated TFPI levels, increased in patients with angina pectoris compared with patients with chest pain syndrome. Increased endothelial cell associated TFPI was associated with hypercoagulability in patients with angina pectoris. These may help to explain the reduction in thrombotic risk associated with the use of heparin.

Angina Pectoris↗
PubMed2000

Expression of tissue factor pathway inhibitor in human fetal and placental tissues.

The coagulation system differs markedly between fetuses, neonates, and adults, and fetal and neonatal thrombotic abnormalities are poorly understood. Tissue factor pathway inhibitor (TFPI) is an important inhibitor of the extrinsic pathway of coagulation. To begin examining the role of TFPI in the maternal-fetal relationship, TFPI expression in human fetal and placental tissues was studied by immunohistochemical staining. TFPI was widely expressed in human fetal tissues from 8-24 weeks gestation, particularly in epithelial and endothelial tissues. Fetal liver, lung, kidney, and intestine were notably positive for TFPI staining. Within the tissues, TFPI was expressed in pneumocytes, hepatocytes, renal tubular and glomerular cells, muscle fibers, and enterocytes. In placental tissues, TFPI was expressed in syncytiotrophoblasts, cytotrophoblasts, vascular endothelium, and extravillus trophoblasts from 10 weeks through term. Advancing gestation had little effect on TFPI expression. Further studies are needed to determine the functional role of TFPI in fetal and placental tissues and to define perturbations in its expression during fetal and neonatal disease states.

Endothelium↗
PubMed1997

Advances in tissue engineering of blood vessels and other tissues.

Tissue engineering is a new and rapidly expanding field, in which techniques are being developed for culturing a variety of tissues both in vitro and in vivo using polymer 'scaffolds' to support tissue growth. Polymer scaffolds used in tissue engineering are generally biodegradable, often involving compounds which are already approved for human implantation. In some cases, these polymers may be chemically modified to exhibit selective cell adhesion properties, which enhance cell attachment and subsequent tissue growth. Many cell types have been successfully cultured on these scaffolds, including smooth muscle cells, endothelial cells, hepatocytes and chondrocytes. Tissue engineering holds the potential for the in vitro development of autologous or allogeneic transplantable vascular conduits. Each year in the USA, there are approximately 1.4 million procedures performed which require arterial prostheses. Most of these procedures are in small calibre (< 6 mm) vessels, for which synthetic graft materials are not generally suitable. While autologous venous or arterial vessels are generally used, not all patients possess adequate conduit for revascularization. Tubular scaffolds have been specially designed for culturing small calibre arteries in vitro. Bovine aortic vascular cells were seeded and cultured on these polymer scaffolds, and grown under conditions of pulsatile pressure and intra-luminal flow. To minimize contamination during the weeks of tissue culture required to produce an arterial prosthesis, a sterile incubator system was developed. Preliminary studies have achieved good cell densities of both smooth muscle cells and endothelial cells on biodegradable polymer scaffolds.

Animals↗
PubMed1995

Analysis of time courses of metabolic precursors and products in heterogeneous rat brain tissue: limitations of kinetic modeling for predictions of intracompartmental concentrations from total tissue activity.

The efficacy of various kinetic models to predict time courses of total radioactivity and levels of precursor and metabolic products was evaluated in heterogeneous samples of freeze-blown brain of rats administered [14C]deoxyglucose ([14C]DG). Two kinetic models designed for homogeneous tissues, i.e., a no-product-loss, three-rate-constant (3K) model and a first-order-product-loss, four-rate-constant (4K) model, and a third kinetic model designed for heterogeneous tissues without product loss [Tissue Heterogeneity (TH) Model] were examined. In the 45-min interval following a pulse of [14C]DG, the fit of the TH Model to total tissue radioactivity was not statistically significantly better than that of the 3K Model, yet the TH Model described the time courses of [14C]DG and its metabolites more accurately. The TH- and 4K-Model-predicted time courses of [14C]DG and its metabolites were similar. Whole-brain glucose utilization (CMRglc) calculated with the TH or 3K Model, approximately 75 mumol 100 g-1 min-1, was similar to values previously determined by model-independent techniques, whereas CMRglc calculated with the 4K Model was 44% higher. In a separate group of rats administered a programmed infusion to attain a constant arterial concentration of [14C]DG that minimizes effects of tissue heterogeneity as well as any product loss, CMRglc calculated with all three models was 79 mumol 100 g-1 min-1 at 45 min after initiation of the infusion. Statistical comparisons of goodness of fit of total tissue radioactivity were, therefore, not indicative of which models best describe the tissue precursor and product pools or which models provide the most accurate rates of glucose utilization.

Animals↗
PubMed2003

TT virus has a ubiquitous diffusion in human body tissues: analyses of paired serum and tissue samples.

The tissue tropism and possible correlation with liver disease of the TT virus (TTV) as well as its prevalence and genotype distribution remain undefined. TTV-DNA was investigated in paired sera and tissue samples from 144 patients, and sera and cerebrospinal fluids (CSF) from additional six subjects. Of the 144 tissue samples, 128 were liver biopsy specimens from subjects with hepatic disease while 16 were surgically obtained nonliver specimens from patients with extrahepatic disease. TTV cloning, sequencing and genotype analyses were performed on isolates from sera, tissue specimens and peripheral blood mononuclear cells of two patients with hepatic and four patients with extrahepatic pathologies, as well as from sera and CSFs of two subjects. TTV was found in 100% of the examined tissues and in 60.1 and 50% of sera from patients with hepatic and extrahepatic pathologies, respectively. Moreover, TTV was detected in four of the six CSFs analysed but only in two correspondent sera. Genotyping revealed the coexistence of multiple TTV genotypes and genetic variants in each infected individual, and the analysis of TTV mRNA showed the presence of transcripts in all the six different tissues studied. These results indicate that the entire adult population in our area is more likely infected by TTV, although several subjects are not viraemic and that TTV infects many different human tissues and is able to invade the central nervous system.

Adult↗
PubMed1991

A detailed description of soft tissue morphology in unilateral cleft lip and palate cases--a study applying a new soft tissue analysis.

More than 1,500 CLP patients have been treated in Berne using a protocol which has always remained fundamentally the same. Even though--from birth to 20 years of age--the finest possible plastic surgery, orthodontics and prosthodontics were undertaken, the specialists involved considered that the patient, at the end of treatment, still possessed a CLP appearance to some extent. Neither the cephalometric analyses, nor the occlusal findings, however, provided any explanation for this, and a new method of soft tissue analysis was therefore developed to provide a detailed picture of the tissue overlying the skeletal profile. This involved taking sagittal cephalometric hard tissue landmarks and locating the equivalent points on the soft tissues. The corresponding hard and soft tissue points were then related to each other both horizontally and vertically. In this way, the soft tissue width along the entire profile could be defined together with the angles around the curvature of the face. Such an analysis makes it possible to describe the soft tissue profile in individual cases and to compare cleft and non-cleft populations. In the present study 25 males (mean age 18) and 23 females (mean age 16.7) with unilateral complete CLP were analysed to define in detail the criteria forming the "cleft palate face".

Adolescent↗
PubMed1991

Tissue lactate content and tissue PCO2 in complete brain ischaemia: implications for compartmentation of H+.

It has recently been proposed by Kraig et al. that H+ is compartmentalized during complete ischaemia, with glial cells of hyperglycemic animals becoming excessively acidic (pH approximately 5.2), compared to neurons (pH approximately 6.2). In part, the evidence for that is a discontinuous delta PCO2/delta lactate relationship, with PCO2 precipitously increasing to a constant value at tissue lactate contents exceeding 20 mmol kg-1. Since the previous study was performed in hypercapnic animals, and encompassed only a small number of animals, we studied the relationship between ischaemic tissue lactate content and tissue PCO2 over the lactate range 8-49 mmol kg-1. Tissue glucose content was varied by glucose injection and ischaemia was induced by decapitation. Tissue PCO2 was measured in vitro at 37 degrees C on excised tissue samples. The results showed a continuous rise in tissue PCO2 over the entire range of lactate concentrations measured, with maximal values around 300 mm Hg. The results thus fail to reveal a discontinuous delta PCO2/delta lactate relationship, with PCO2 increasing to a constant value. The implication of the results for the hypothesis of pH compartmentation is discussed.

Animals↗
PubMed2005

Depletion of four nitrofuran antibiotics and their tissue-bound metabolites in porcine tissues and determination using LC-MS/MS and HPLC-UV.

Depletion of the nitrofuran antibiotics furazolidone, furaltadone, nitrofurantoin and nitrofurazone and their tissue-bound metabolites AOZ, AMOZ, AHD and SEM from pig muscle, liver and kidney tissues is described. Groups of pigs were given feed medicated with one of the nitrofuran drugs at a therapeutic concentration (400?mg?kg(-1)) for ten days. Animals were slaughtered at intervals and tissue samples collected for analysis for six weeks following withdrawal of medicated feed. These samples were analysed both for parent nitrofurans (using LC-MS/MS and HPLC-UV), and for tissue-bound metabolites (using LC-MS/MS). The parent drugs were detectable only sporadically and only in pigs subjected to no withdrawal period whatsoever. This confirms the instability of the four major nitrofuran antibiotics in edible tissues. In contrast, the metabolites accumulated to high concentrations in tissues (ppm levels) and had depletion half lives of between 5.5 and 15.5 days. The metabolites of all four drugs were still readily detectable in tissues six weeks after cessation of treatment. This emphasizes the benefits of monitoring for the stable metabolites of the nitrofurans.

Animals↗
PubMed1996

Impedance spectra of tumour tissue in comparison with normal tissue; a possible clinical application for electrical impedance tomography.

Electrical characteristics of living tissues have been investigated for a long time in the search for further methods to complement the traditional investigations of pathology and physiology. Tumour tissue has been shown to exhibit a larger permittivity and conductivity than normal tissues. This might be associated with the fact that tumour cells have a higher water content and sodium concentration than normal cells, as well as different electrochemical properties of their cell membranes. To our knowledge only a few contributions on this subject have been published. This study describes an additional application on measurements of the complex impedance of tumour and normal tissues, in order to compare the impedance features of the two tissue types. The tissue sample is placed in a measuring cell in which the temperature is controlled. The measuring cell is connected to an impedance meter able to measure the complex impedance in terms of real and imaginary part curves for frequencies from 1.5 kHz to 700 kHz. The four-electrode principle is used with the current injected by the outer electrodes and the voltage difference measured between the inner electrodes. The current can be altered up to 1 mA. The instrument can be calibrated with known resistance and capacitance networks connected to the input of the instrument in order to minimize the measurement errors. The calibration routine uses a polynomial adaptation and can be applied interactively. Measurements performed by the instrument show promising results. Preliminary results show that this method can be extended to a new application for detection of tumour tissue by electrical impedance tomography (EIT).

Animals↗
PubMed1980

Reconstruction of the lower extremity with vascularized composite tissue: improved tissue survival and specific indications.

A retrospective assessment of 50 vascularized composite tissue transfers was carried out with 48 patients. Factors responsible for improved tissue survival included (1) the evaluation and proper selection of recipient vasculature, (2) the increased dependence on the vacularized latissimus dorsi musculocutaneous flap, and (3) the frequent use, wherever possible, of an end-to-side arterial anastomisis. Specific indications for reconstruction of the lower extremity with vascularized composite tissue include (1) avulsive injuries to the distal tibia and foot, (2) the failure of conventional methods, (3) the treatment of extensive chronic osteomyelitis, (4) deficiency of both soft tissue cover and skeletal support, (5) the restoration of form and contour with minimal secondary deformity of the donor site, and (6) extensive loss of soft tissue only. Reconstruction of the lower extremity with vascularized tissue is a reliable method with acceptable patient and tissue morbidity statistics that should be considered when specific indications are present.

Amputation, Traumatic↗
PubMed1991

Biochemical and tissue print analyses of hydroxyproline-rich glycoproteins in cell walls of sporophytic maize tissues.

In an effort to understand the role of hydroxyproline-rich glycoproteins (HRGPs) in plant cell wall structure, we studied the distribution and physical properties of PC-1-like proteins (PC-1 being the major pericarp HRGP) throughout sporophytic tissues of two maize (Zea mays L.) varieties. We determined total amounts of hydroxyproline, an indicator of HRGPs, and did tissue print and Western blot analysis. We found hydroxyproline in cell walls of stems, leaves, roots, tassels, and silks. We also observed reactivity of anti-PC-1 monoclonal antibodies with anatomical prints of these tissues on nitrocellulose paper. Stem nodes and silks contained the most hydroxyproline and exhibited the strongest reaction with the antibody. PC-1 was localized in vascular bundles and the epidermis of stem tissue. However, localization to a specific cell type in the silk could not be determined at the resolution of the tissue print. The stem node protein had the same electrophoretic mobility as the pericarp protein as determined on Western blots prepared from cationic neutral gels. Protein extracts from silk tissues of both varieties studied contained one protein of the same size/charge as that found in pericarp, as well as some minor variant bands. The data presented here document that cell wall proteins are present in many tissues of the maize plant, although they are primarily in cell types contributing to support.

Journal Article↗