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[Effect of the triazine herbicide herbazine-50 on the nitrate-nitrite concentration of corn].

Studied were the changes in the nitrate-nitrite content of maize grown on areas treated with herbazine-50 and artificial nitrogen fertilizers. The chemical analysis of various parts of the plant (stem. leaves, flowers and grains) revealed that the content of nitrates and nitrites was highest in the ears, and lowest in the leaves. Highest was also the content of nitrates and nitrites in all parts of the plant in maize from areas treated with herbazine-50 at the rate of 4 kg of a. i. per hectare as well as with artificial nitrogen fertilizers (ammonium nitrate and superphosphate at 450 kg/ha), followed by maize obtained from areas where ammonium nitrate and superphosphate at 450 kg/ha were introduced only. Slightly lower amounts of nitrates and nitrites were found in maize from areas treated with herbazine-50 at 4 kg/ha only, and lowest was their content in maize from areas that were neither fertilized nor treated with herbazine-50.

Herbicides↗

[Clinical and experimental studies of chronic Herbazin-50 poisoning in sheep].

Studied were the changes in some clinical and paraclinical indices in sheep treated with the triazine preparation herbazin-50 used at the rate of 1.4 mg per kg of live weight. The effect was also followed up of this dose on the course of pregnancy, the general health status of the newborn lambs, and the deposition of the preparation in the liver and the musculature of the fetus.

Animals↗

Cyanuric acid--a s-triazine derivative as a nitrogen source for some soil microorganisms.

Cyanuric acid was not toxic for soil microorganisms examined and was even observed to stimulate the growth of Azotobacter in chernozem. Some isolated fungi were capable of cleaving the ring of cyanuric acid. With the use of 15N-labeled cyanuric acid it was found that the nitrogen taken from this compound by Aspergillus minutus and Pseudogymnoascus sp. was incorporated into their proteins. About 70-90% of 15N derived from cyanuric acid was detected in the biomass of the examined fungi. The ability of soil microorganisms to cleave the triazine ring is of importance in the detoxication of soils treated with triazine herbicides.

Bacteria↗

[Effect of herbazin 50 on the course of suppurative surgical infection in sheep].

Twelve sheep, divided into two groups of 6 animals each, were used. The first (test) group was offered herbazin -50 with the feed in the course of 90 days after which the animals were infected via the joints with Corynebacterium pyogenes. The second (control) group was also infected with the same pathogen in the same way. During the time the preparation was given and 30 days post infection the animals were kept under observation, following up their clinical, condition, and the occasional morphological and biochemical changes taking place in the blood and the immunobiologic responsiveness. It was found that sheep treated with herbazin -50 with a following infection with Corynebacterium pyogenes raised their blood levels of sugar, total protein, potassium, sodium, magnesium, SGOT, SGPT, and manifested an increased ESR, and lower calcium, inorganic phosphorus, carotene, vitamin A, and cholesterine serum level. It was also established that surgical infections brought about through such introduction of C. pyogenes developed much more rapidly and ran a much more severe course in sheep after the preliminary treatment with herbazin -50. It is believed that they emerged after suppressing the immunologic responsiveness with the use of the preparation.

Animals↗

[Effect of heavy metals and pesticides on the antigenic behavior of mastitis staphylococci isolated from cows and sheep].

Evaluated was the effect produced by some heavy metals and pesticides on the antigenic behaviour of Staphylococcus aureus strains isolated from cows and sheep with mastitis. The experiments were carried out with 9 strains, their antigenic structure being determined with the use of 16 factor sera prior to and after being gradually acted upon with lead acetate, copper sulfate, zinc sulfate, cadmium, and herbazin-50. It was found that under the effect of these chemical factors the capsule-resembling layer grew. In connection with this the staphylococci grew within the nutrient media, forming mucoid colonies. The serologic analysis revealed lower agglutinin ability due to the release of deeply set antigens. The growth of the capsular layer was associated with the increase in the virulence of staphylococci, which was established in the conditions of the practice on a dairy farm adjacent to a Copper producing Works.

Animals↗

[Embryotropic action of the triazine herbicide polyzin 50].

The embryotropic effect of the new Bulgarian simtriazine herbicide polyzin 50 was studied in an experiment upon 90 pregnant albino rats - Wistar, via inhalation effect in concentrations 2 mg/m3 and 0.2 mg/m3, daily during the whole pregnancy and at a level of 2 mg/m3 during the first trimester of gestation. With both regimes, the preparation has a teratogenic and a more lightly manifested embryotoxic activity. In the absence of general toxic effect upon the pregnant organism, polyzin induces external malformations (hypognathus, oedema), anomalies in the development of the internal organs (hydrocephalus, intracerebral hematoma, hemorrhages in the abdominal cavity, infarctus-like foci in liver) and defects in the ossification of the cranial bones of fetus. The preparation has an embryotoxic activity (increased incidence of subcutaneous hematomas of the fetus), only with the daily penetration in organism of the pregnant animals in a concentration of 2 mg/m3. With both concentrations of antenatal effect of polyzin studied, a functional insufficiency of the liver of the progeny develops during the postnatal development period. On the grounds of the data obtained about the embryotoxic and teratogenic effect of polyzin with its inhalation effect in low concentrations - 2 mg/m3 and 0.2 mg/m3, a recommendation is proposed-prohibition of women's participation in its production at a fertile age.

Animals↗