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At least 451 records · Page 25Linked to original sources
PubMed1996

Attempt to obtain greater dermal depth of vascular injury using dye-enhanced laser technique: a new approach.

BACKGROUND AND OBJECTIVE: Although pulsed dye laser has been successfully used in the treatment of portwine stains, a number of patients had incomplete clearance because the depth of penetration by the pulsed dye laser was inadequate. This study was performed to establish the greater penetration depth of vascular injury using a dye-enhanced laser technique. STUDY DESIGN/MATERIALS AND METHODS: The ruby laser at 694.3 nm was used, and the corresponding specific dye was Prussian blue solution (maximum absorbance 694 nm). We compared the penetration depth of vascular injury by the ruby laser irradiation after the Prussian blue injection with that by the dye laser irradiation. A flashlamp dye laser with a pulse duration of 300 microseconds and a 5 mm diameter spot size was used to 6.2 J/cm2 at 590 nm. The Prussian blue solution was injected into the superficial epigastric artery of white male Japanese rabbits, immediately followed by the ruby laser exposure to 6.2 J/cm2 at a pulse duration of 283 microseconds in a 15 x 15 mm spotsize. Depth of penetration was measured from the dermoepidermal junction to the deepest site of vascular injury at 24 hours after laser exposure. RESULTS: Mean penetration of 590 nm of the dye laser light was 1.45 mm; mean penetration of the 694.3 nm ruby laser irradiation after the Prussian blue injection was 2.33 mm. Ruby laser penetration was greater than that of the dye laser. CONCLUSION: This study emphasizes that the ruby laser irradiation after the Prussian blue injection can induce deeper vascular injury than the dye laser inducing similar pathological changes.

Animals↗
PubMed2005

Cyanogenic glucosides in grapevine: polymorphism, identification and developmental patterns.

Twelve grapevine (Vitis vinifera L.) cultivars were surveyed for 'cyanide potential' (i.e. the total cyanide measured in beta-glucosidase-treated crude, boiled tissue extract) in mature leaves. Two related cultivars (Carignan and Ruby Cabernet) had mean cyanide potential (equivalent to 110 mgHCNkg-1fr.wt) ca. 25-fold greater than that of the other 10 cultivars, and so the trait is polymorphic in the species. In boiled leaf extracts of Carignan and Ruby Cabernet, free cyanide constituted a negligible fraction of the total cyanide potential because beta-glucosidase treatment was required to liberate the major cyanide fraction - which is therefore bound in glucosylated cyanogenic compound(s) (or cyanogenic glucosides). In addition, cyanide was liberated from ground leaf tissue of Ruby Cabernet but not Sultana (a cultivar with low cyanide potential). Hence, the high cyanide potential in Ruby Cabernet leaves is coupled with endogenous beta-glucosidase(s) activity and this cultivar may be considered 'cyanogenic'. A method was developed to detect and identify cyanogenic glucosides using liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). Two putative cyanogenic glucosides were found in extracts from leaves of Carignan and Ruby Cabernet and were identified as the epimers prunasin and sambunigrin. Cyanide potential measured at three times over the growing season in young and mature leaves, petioles, tendrils, flowers, berries, seeds and roots of Ruby Cabernet was substantially higher in the leaves compared with all other tissues. This characterisation of cyanogenic glucoside accumulation in grapevine provides a basis for gauging the involvement of the trait in interactions of the species with its pests and pathogens.

Chromatography, Liquid↗
PubMed1997

Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates.

The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely labeled motoneurons was found with Mini-Ruby, Fluoro-Gold and Fluoro-Ruby, while Fluoro-Emerald yielded a smaller number of labeled cells. With increasing survival time, all of these tracers exhibited a marked decrease in the number of labeled neurons. Fast Blue also produced very efficient staining after 1 week and, in addition, the number of Fast Blue-labeled cells remained constant over the entire time period studied. Also in embryonic spinal cord tissue exposed to Fast Blue. the label persisted for at least 6 months after transplantation into adult spinal cord. Double-labeling experiments combining Fast Blue with Fluoro-Gold, Mini-Ruby, Fluoro-Ruby or Fluoro-Emerald showed that all these substances were non-toxic and that the time-related decrease in the number of neurons labeled by the latter tracers was due to degradation or leakage of the dyes. Thus, Fast Blue would be the tracer of choice for motoneuronal labeling in long-term experiments, whereas the usage of the other tracers should be restricted to experiments of limited duration.

Amidines↗