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[Minute chromosome rearrangement detected by human telomeric band painting probes].

In order to assess the value of applying human chromosomal telomeric band painting probes (TBPs) in genetic diagnosis, we used three human telomeric band painting probes in fluorescence in situ hybridization (FISH) to analyze two cases who had a history of habitual abortion and were suspected to carrying a minute translocation. The probes were prepared by microdissection, and the covered regions were 11q23.3-->qter12q24.1-->qter and 22q13.1-->qter, respectively. According to the FISH results, both the cases were cryptic minute translocation carriers. The involved chromosomes were chromosome 11 and 12 in case 1, and chromosome 11 and 22 in case 2. Combined with chromosome G banding, their breakpoints were 11q23.3, 12q24.1, and 22q13.1, respectively. The results showed that the telomeric band painting probes can be used as a tool to determine minute chromosomal abnormality and the precise breakpoints.

Abortion, Habitual↗

Acute effect of indoor exposure to paint containing bis(tributyltin) oxide--Wisconsin, 1991.

In January 1991, a woman in Wisconsin contacted her local public health department to report that she and her two children had become ill after her landlord painted the walls and ceilings of two rooms of her apartment. Reported symptoms included a burning sensation in the nose and forehead, headache, nose bleed, cough, loss of appetite, nausea, and vomiting. The woman, who was in the third trimester of pregnancy, also complained of a persistent odor from the paint and provided an empty bottle of a paint additive used for mildew control. The label indicated that this product contained 25% bis(tributyltin) oxide (TBTO) as its only active ingredient.

Acute Disease↗

[Modelling the transfer of pollutants from painted surfaces to the surrounding air].

As dried paintings may emit pollutants into indoor air by evaporation or sublimation processes, men living in such rooms my incorporate a certain amount of these substances via the respiratory tract or via the skin according to the pollutant concentration. Furthermore, foodstuff and feeds may as well absorb such substances. The chronic toxicity of the single compound and its daily up- take by man determine the risk of a possible danger to human health. For the toxicological evaluation of pollutants emanating from paints it is necessary that procedures are available to estimate the maximum indoor air concentration of these pollutants. Model experiments are described and equations are given for the calculation of the equilibrium concentration of pollutants occurring in indoor air after the application of paints. The validity of the equations has been ascertained taking as examples different fungicides and pesticides. The concentration values from model experiments were in good agreement with the results of field measurements.

Air Pollutants↗

[Chemical and physical reactions during the drying process of paints and their impact on indoor air quality].

Taking six different cases of paint application indoors (three "do-it-yourself" workers and three professional painters), the maximum solvent concentration to be expected in the indoor air is calculated. The results of gas chromatographic determinations of the emission of solvents from paints and lacquers and of old lacquer layers are used to show that the paints offered today to a wide extent fulfill the requirements of a protection of the environment and do not represent a danger to the user and the inhabitant of a room provided that one is ready to follow the instructions and security advices. The importance of a sufficient air exchange rate is stressed.

Air Pollutants↗

Toxic pyrolysis products of solvents, paints, and polymer films.

The organic chemicals used for solvents, paint, and polymer films are resistant to heat, but if the temperature is raised sufficiently, all will pyrolyze or degrade thermally to form new, lower-molecular-weight chemical species. The solvents include nonhalogenated and halogenated varieties, and their possible occupational exposures and reaction products are described. Paint and paint-like coatings, when pyrolyzed, always produce smoke, but toxic metals included as pigment may produce hazardous fumes. The condition "meat wrapper's asthma" is described to illustrate the effects once believed due to PVC pyrolysis.

Air Pollutants, Occupational↗

[The painting 'The anatomical lesson by Professor Louis Bolk' by Martin Monnickendam].

In 1992 the painting 'The anatomy of professor Louis Bolk' by the Dutch painter Martin Monnickendam (1874-1943), was finally returned to its place in the 'Tulp-room' of the Department of Anatomy and Embryology at the University of Amsterdam, after the department had been moved to new premises in 1985. The professor of anatomy at the University of Amsterdam, Louis Bolk, is seated in the middle surrounded by his, at that time, former pupils, from left to right: Boeke, Barge and van den Broek, authors of a Dutch textbook of anatomy. The four professors are gathered around a cadaver of an orang-utan, placed not on a dissection table but on a console. Bolk is facing the observer and holding a scalpel in one hand while steadying the animal's head with the other; he is ready to make an incision. In the back of the painting a bust of the 18th century anatomist Petrus Camper is visible. The four professors all wear a white coat over their suits. Bolk finishes this off with an artistic bow tie whilst the others use an ordinary necktie. The painting is signed in the left upper corner 'Martin Monnickendam 1925'.

Famous Persons↗

A reconstructive surgeon's taste in art: Dr Peter Parker and the Lam Qua oil paintings.

Hidden within the basement archives of Yale University's Historical Medical Library lie the original oil painting collection and personal papers of the first American surgeon to practice in China. The Reverend Dr Peter Parker, a graduate of Yale, established the first American hospital in Guangzhou (formerly Canton) in 1835 and successfully introduced Western surgical techniques including amputation, anesthesia, and reconstructive surgery. In addition to this distinguished career of medical missionary service, Parker commissioned the Chinese artist Lam Qua to paint a fascinating series of portraits depicting patients with extreme tumor growths. In this article, Parker's own journals are examined to reveal both his missionary zeal and his frustrations working in China. Selected examples of the Lam Qua paintings are presented along with Parker's corresponding patient descriptions. These detailed patient reports give insight into the extent of surgical pathology as well as the relative brutality of surgical techniques in the early to mid-1800s. The historical significance of the Peter Parker-Lam Qua collaboration within the context of the Western transformation of Chinese art and medicine is also discussed.

China↗

Compatibility of army face paint and insect repellent formulation.

The compatibility studies of a new controlled-release repellent formulation (NSN 6840-01-284-3982, insect repellent personal application) and a new camouflage face paint (NSN 6850-01-262-0635) indicated that the repellent did not interfere with the use of face paints when repellent was applied first, and the face paints did not reduce the effectiveness of the repellent against mosquitoes.

Aedes↗

Evaluation of paintings of nonretarded and retarded persons by judges with and without art training.

Correlations between creativity and intelligence are generally reported to be low. In the present investigation, we examined whether judges with and without art training (N = 40 for each group) would evalute the paintings of nonretarded and retarded persons differently. Five paintings each were obtained from 15 nonretarded persons, 11 moderately retarded persons, and 11 severely retarded persons. Judges who had art training did not differentially rate the art of nonretarded and retarded persons, but they gave significantly higher ratings than did non-art-trained judges to the paintings of retarded persons.

Adolescent↗

[Physician's role in Norwegian art of painting].

The author reviews paintings of doctors by Norwegian artists. Many interesting pictures have been painted, especially during the era of naturalism at the end of the last century. The artist and journalist Christian Krohg contributed to an early discussion of the role of the doctors in connection with social problems in Norway. In the 1930s, Finn Faaborg illustrated the relation between doctor and patient in the case of tuberculosis, and Carl von Hanno revived the problem of prostitution. Bendik Riis, himself a psychiatric patient, with his huge painting Castraktion, shows his fear of castration and lobotomy, and also his opinion of the health authorities' lack of respect for the patient. Norwegian painters have obviously contributed to a fruitful discussion of the role of the doctor in society.

History, 19th Century↗

Eye injuries during training exercises with paint balls.

The use of paint balls, increasingly popular for war games and survival games, has recently been introduced for certain military training exercises. We treated two soldiers who had severe permanent ocular damage after being hit by these balls. Both removed their goggles after their vision was obscured with paint from a hit on these protective lenses. They were then hit for a second time, resulting in the eye injury. Based on these cases and on a literature review, we conclude that protective eye gear should be mandatory when paint pellets are used. It is essential that service members participating in such exercises be instructed not to remove their protective goggles under any circumstances.

Adult↗

The application of a standard color coding system to paint in forensic science.

The procedure described for the measurement of paint colors represents an attempt to standardize the measurement of color in the forensic science service and provides a means of communicating colors between individuals. The effect of sample size on color comparisons has been discussed. It has been shown that color data collected from many control samples of paint flakes can provide the frequency of occurrence of paint colors. The errors involved in color comparisons have been assessed and the system has been shown to be a practicable one.

Color↗

Examination of paints by trace element analysis.

Trace element analysis using neutron activation analysis is effective and valuable, particularly in the examination of white household paint. Although physical appearance and resin composition are generally similar in these paint samples, trace element composition provides an effective way of distinguishing among them. In the case of automobile paint samples, NAA serves as an important additional technique for discrimination. The technique is important when sample sizes are very small. The technique developed takes a few minutes for sample preparation, a few hours of irradiation time (during which the examiner's presence is not required), and then a few minutes for counting and obtaining quantitative multielement concentration patterns. A technician can easily handle 30 to 50 samples per day.

Neutron Activation Analysis↗

The chemoprotective effect of coenzyme Q on lipids in the paint and lacquer industry workers.

The influence of coenzyme Q on the lipid parameters in the paint and lacquer industry workers is presented. The examinations were carried out in the group of 24 workers employed at the paint and lacquer production, who received coenzyme Q10 as a chemoprotective agent. Serum concentration of basic lipid parameters: total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), triglycerides (TG); lipid peroxidation products: malonyldialdehyde (MDA) together with 4-hydroxynonenal (4-HNE) and two antioxidant enzymes: superoxide dismutase (SOD) and glutathione peroxidase (GPx) were examined. The above parameters were measured in workers exposed to organic solvents and then after 4 weeks of coenzyme Q treatment. In order to explain, whether the occupational exposure is responsible for the changed level of some parameters, the reference group, not employed in the paint and lacquer industry, was used. The results indicated that the preliminary blood serum concentration of MDA + 4-HNE in workers exposed to organic solvents was significantly elevated in comparison to the control group. Statistically significant decrease in MDA + 4HNE concentration was observed after coenzyme Q treatment what lead to the conclusion that coenzyme Q could be considered as a protective agent against lipid peroxidation in occupational exposure. The changes in other parameters were statistically insignificant.

Adult↗

Diagnosis of four chromosome abnormalities of unknown origin by chromosome microdissection and subsequent reverse and forward painting.

A molecular cytogenetic method consisting of chromosome microdissection and subsequent reverse/forward chromosome painting is a powerful tool to identify chromosome abnormalities of unknown origin. We present 4 cases of chromosome structural abnormalities whose origins were ascertained by this method. In one MCA/MR patient with an add(5q)chromosome, fluorescence in situ hybridization (FISH), using probes generated from a microdissected additional segment of the add(5q) chromosome and then from a distal region of normal chromosome 5, confirmed that the patient had a tandem duplication for a 5q35-qter segment. Similarly, we ascertained that an additional segment of an add(3p) chromosome in another MCA/MR patient had been derived from a 7q32-qter segment. In a woman with a history of successive spontaneous abortions and with a minute marker chromosome, painting using microdissected probes from the whole marker chromosome revealed that it was i(15)(p10) or psu dic(15;15)(q11;q11). Likewise, a marker observed in a fetus was a ring chromosome derived from the paracentromeric region of chromosome 19. We emphasize the value of the microdissection-based chromosome painting method in the identification of unknown chromosomes, especially for marker chromosomes. The method may contribute to a collection of data among patients with similar or identical chromosome abnormalities, which may lead to a better clinical syndrome delineation.

Abnormalities, Multiple↗

Prevention of marine biofouling using a conductive paint electrode

Conductive paint electrode was used for marine biofouling on fishing nets by electrochemical disinfection. When a potential of 1.2 V vs. a saturated calomel electrode (SCE) was applied to the conductive paint electrode, Vibrio alginolyticus cells attached on the electrode were completely killed. By applying a negative potential, the attached cells were removed from the surface of the electrode. Changes in pH and chlorine concentration were not observed at potentials in the range -0.6 approximately 1.2 V vs. SCE. In a field experiment, accumulation of the bacterial cells and formation of biofilms on the electrode were prevented by application of an alternating potential, and 94% of attachment of the biofouling organisms was inhibited electrically on yarn used for fishing net coated with conductive paint. Copyright 1998 John Wiley & Sons, Inc.

Journal Article↗

The role of the integument as a barrier to penetration of ice into overwintering hatchlings of the painted turtle (Chrysemys picta).

Hatchlings of the North American painted turtle (Chrysemys picta) spend their first winter of life inside a shallow, subterranean hibernaculum (the natal nest) where they may be exposed for extended periods to ice and cold. Hatchlings seemingly survive exposure to such conditions by becoming supercooled (i.e., by remaining unfrozen at temperatures below the equilibrium freezing point for body fluids), so we investigated the role of their integument in preventing ice from penetrating into body compartments from surrounding soil. We first showed that hatchlings whose epidermis has been damaged are more likely to be penetrated by growing crystals of ice than are turtles whose cutaneous barrier is intact. We next studied integument from a forelimb by light microscopy and discovered that the basal part of the alpha-keratin layer of the epidermis contains a dense layer of lipid. Skin from the forelimb of other neonatal turtles lacks such a layer of lipid in the epidermis, and these other turtles also are highly susceptible to inoculative freezing. Moreover, epidermis from the neck of hatchling painted turtles lacks the lipid layer, and this region of the skin is readily penetrated by growing crystals of ice. We therefore conclude that the resistance to inoculation imposed by skin on the limbs of hatchling painted turtles results from the presence of lipids in the alpha-keratin layer of the epidermis. Neonates apparently are able to avoid freezing during winter by drawing much of the body inside the shell, leaving only the ice-resistant integument of the limbs exposed to ice in the environment. The combination of behavior and skin morphology enables overwintering hatchlings to exploit an adaptive strategy based on supercooling.

Adaptation, Physiological↗

Effect of skin painting with oxazolone on the local extravasation of mononuclear cells in sheep.

A characteristic feature of the induction of cell-mediated delayed hypersensitivity reactions by chemicals such as oxazolone is the enlargement of lymphocyte traffic areas in the paracortices of regional lymph nodes. In sheep oxazolone is a powerful immunogen but the cellular changes in lymph efferent from nodes draining areas of oxazolone-painted skin do not differ significantly from responses to conventional antigens. Specific complement-binding antibodies appear in the plasma of sensitized sheep, which respond to secondary challenges with an immediate Arthus reaction. In studies of peripheral lymph from areas of skin painted with oxazolone the number of mononuclear cells in the lymph increased 10--50-fold two days or so after skin painting. Most of these cells were small lymphocytes lacking surface immunoglobulin (presumptive 'T' cells). This big increase in lymphocyte traffic through the skin may be a consequence of the binding to local structural proteins of myriads of oxazolone epitopes. If so, and bearing in mind the large doses of immunogen used in experiments on mice, it is easy to envisage how the traffic areas of lymph nodes expand and become congested with lymphocytes after being flooded with a highly immunogenic and reactive chemical like oxazolone. Whether this is relevant to the induction of cell-mediated immunity is unknown.

Animals↗