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PubMed1982

The purification and characterization of alpha-L-fucosidase from the hepatopancreas of Octopus vulgaris.

We have isolated and purified, by affinity chromatography with Agarose-epsilon-amino-caproyl-fucosamine, an alpha-L-fucosidase [alpha-L-fucoside fucohydrolase EC 3.2.1.51] from the hepatopancreas of Octopus vulgaris. In the purified fraction only fucosidase activity could be detected. However, two protein bands, one major (about 95 per cent) and one minor (about 5 per cent), were evident on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Isoelectric focusing also revealed two activities, pI 8.1 (major) and pI 7.3 (minor). Under denaturing conditions the molecular weight of the major band was estimated to be 52,000 while that of the minor one was 43,000. Only one major activity peak with an apparent molecular weight of 70,000--75,000 was detected by gel filtration chromatography. The enzyme has two optimal pH values, and the relative activities are temperature-dependent; one optimum is at pH 5.5 +/- 0.2 and the other at pH 3.0 +/- 0.2. We found that the enzyme has a maximum activity at about 70 degrees C, but 50 per cent of the enzyme was inactivated at 70 degrees C after 5 min. The purified enzyme, using p-nitrophenyl-L-fucoside as substrate, has a specific activity of 38.9 units/mg of protein, Km of 3.58 x 10(-4) M and Vmax of 65 mumol/min/mg of protein. alpha-L-Fucose acts as a competitive inhibitor, with a K1 of 1.2 x 10(-3) M. alpha-L-Fucosidase released radioactive fucose from cellular glycopeptides, but no detectable free fucose was released fom 5 natural substrates.

Animals↗
PubMed1986

Octopus bite resulting in granuloma annulare.

The cause of granuloma annulare is unclear. I have reported the development of characteristic clinical and histologic lesions of granuloma annulare at the site of an octopus bite, with early signs following the bite by two weeks.

Bites and Stings↗
PubMed1979

Antigen-induced secretion in the optic gland of Octopus vulgaris.

The optic gland of the octupus responds to a variety of foreign proteins--1 to 2 days after the injection--by the mass production and release of a particulated material. In the electron microscope the antigen-induced substance shows as a non-membrane bound particle of size about 30 nm. When released into the blood it does not disintegrate instantly. The chemistry of this material, which is probably identical with the optic gland hormone, is not known. However, non-vesicular storage and release as well as the abundance of tubular mitochondria suggest that the hormone is a steroid. The optic gland hormone, which is known to control sexual maturation, feeding and death in Octopus, appears to be involved in a defence mechanism against non-octopus proteins.

Animals↗
PubMed1992

[14C]deoxyglucose labelling of functional activity in the cephalopod central nervous system.

For the first time, the [14C]deoxyglucose radioautographic technique has been successfully used to map physiological activity in cephalopod brains. In unilaterally blinded octopus and cuttlefish, the optic lobe of the deprived side showed a decreased uptake of the labelled tracer. This suggests that the uptake is related to functional activity. The potential of the [14C]deoxyglucose technique as a powerful tool in studying the functional organization of cephalopod brains is discussed.

Animals↗