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At least 451 records · Page 25Linked to original sources
PubMed1981

One to one correlation of histological and histochemical light microscopy with scanning electron microscopy.

Several histological stains were applied to specimens after scanning electron microscopic (SEM) processing. Histochemical stains were applied before SEM fixation. After staining, the specimens were processed and dried by SEM techniques. The specimens were taped to a microslide, specimen side up, scribed and covered with immersion oil. After light micrography (LM), the oil was removed and the specimens mounted and gold coated. The same cells were then relocated and photographed by SEM. Periodic acid Schiff was not usable as a specific tissue aldehyde stain, but did prove to be a useful counterstain for dehydrogenase stained specimens. Colloidal iron, as seen by SEM, resulted in a non-specific granular deposit over the specimen and the substrate. All the other histological stains examined--alcian blue, Grams, Feulgen and toluidine blue--were specific and did not change the specimen ultrastructure. The histochemical stains--acid and alkaline phosphatase and three dehydrogenases--were also specific. There were some minor ultrastructural changes with the AcPase and AlPase stains. However, the tissue surface was not significantly distorted. These and other techniques should prove to be a useful adjunct to SEM studies.

Amniotic Fluid↗
PubMed1996

Confocal in vivo microscopy and confocal laser-scanning fluorescence microscopy in keratoconus.

The purpose of this study was the determination of morphological changes in the corneal epithelium and the keratocyte network in keratoconus. In all, 33 eyes of 19 patients were examined in vivo using the confocal slit-scanning microscope Microphthal. After penetrating keratoplasty, recipients' trephanates were stained with the Live/Dead kit and examined using the confocal laser-scanning fluorescence microscope Diaphot 300/Odyssey. The fluorescence images were reconstructed three-dimensionally. All findings were compared with data from healthy corneas. Morphological alterations were found only in the area of the corneal apex; obviously elongated superficial epithelial cells arranged in a whorl-like fashion were found. Near Bowman's membrane, highly reflective changes and fold-like structures were visible. The anterior stroma also showed an increased reflectivity. In the posterior stroma, typical findings were Vogt's striae and keratocytes with extremely long processes arranged nearly in parallel. In scarred stroma the keratocytes were spindle-shaped and arranged irregularly. The spatial organization of the living keratocyte network could be demonstrated through three-dimensional reconstructions.

Adult↗