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[Duodenum-preserving total pancreatic head resection and pancreatic head resection with segmental duodenostomy].

A duodenum-preserving pancreatic head resection (DPPHR) was first reported by Beger et al. in 1980. However, its application has been limited to chronic pancreatitis because of it is a subtotal pancreatic head resection. In 1990, we reported duodenum-preserving total pancreatic head resection (DPTPHR) in 26 cases. This opened the way for total pancreatic head resection, expanding the application of this approach to tumorigenic morbidities such as intraductal papillary mucinous tumor (IMPT), other benign tumors, and small pancreatic cancers. On the other hand, Nakao et al. reported pancreatic head resection with segmental duodenectomy (PHRSD) as an alternative pylorus-preserving pancreatoduodenectomy technique in 24 cases. Hirata et al. also reported this technique as a new pylorus-preserving pancreatoduodenostomy with increased vessel preservation. When performing DPTPHR, the surgeon should ensure adequate duodenal blood supply. Avoidance of duodenal ischemia is very important in this operation, and thus it is necessary to maintain blood flow in the posterior pancreatoduodenal artery and to preserve the mesoduodenal vessels. Postoperative pancreatic functional tests reveal that DPTPHR is superior to PPPD, including PHSRD, because the entire duodenum and duodenal integrity is very important for postoperative pancreatic function.

Adenocarcinoma, Mucinous↗

Clip-assisted endoscopic method for placement of a nasoenteric feeding tube into the distal duodenum.

Gastroenterologists are often frustrated in their efforts to deliver a feeding tube by endoscopic guidance into the small bowel because of retrograde migration during the withdrawal of the endoscope. We describe a clip assisted endoscopic method whereby a nasoenteric feeding tube can be reliably delivered into the distal duodenum. A nasoduodenal tube with a 3-0 silk suture sewn on its distal tip is inserted into the stomach. The suture on the feeding tube is grasped by a clip-fixing device. Then, the endoscope with feeding tube is advanced into the distal duodenum and the tube is fixed on a mucosal fold by clipping. We used this technique to successfully place nasoenteric tubes into the distal duodenum in 9 patients. There were no procedure-related complications, and no bleeding or perforation due to removal of the feeding tubes was observed. We conclude that this clip-assisted endoscopic method is a reliable modality for placing nasoenteric tubes.

Adult↗

Influence of calcium removal from bath solution on isolated rat duodenum contractions.

In this study whether extracellular Ca++ is essential to produce an increase of tension of isolated rat duodenum by ACh, 5-HT, AVP and KCl-, was examined. KCl- and AVP-evoked contractions were almost totally prevented by Ca++ removal from bath solution. The increase of tension of isolated duodenum caused by ACh or 5-HT was totally prevented after adding nifedipine, a Ca++ channel blocker, into Ca free solution. Our results suggest that ACh or 5-HT utilizes intracellular as well as extracellular sources of Ca++ to produce contraction in rat duodenum, whereas AVP-or KCl evoked contraction was mainly due to influx of Ca from extracellular sources.

Acetylcholine↗

Abnormalities of the autonomic nervous system of the duodenum in streptozotocin-induced diabetic rats.

The changes in sensitivity of the rat duodenum to ATP were examined in preparations from control rats and from rats with streptozotocin (STZ)-induced diabetes. ATP was able to relax the duodenum of control animals in a concentration-dependent manner and this relaxation was significantly increased in STZ-diabetic rats. In preparations from diabetic rats, relaxation responses to ATP were greater than those of age-matched control groups. These results suggest that functional changes or degeneration of non-adrenergic, non-cholinergic nerves in the myenteric plexus may occur in STZ-diabetic rats, and that these alterations may result in the increased sensitivity of the relaxation of the duodenum to ATP.

Adenosine Triphosphate↗

Age- and disease-related methylation of multiple genes in nonneoplastic duodenum and in duodenal juice.

PURPOSE: Methylation of CpG islands contributes to gene silencing during cancer development, and although some methylation alterations are promising diagnostic markers of cancer, some CpG islands are also methylated in normal tissues. We have previously observed that some normally unmethylated CpG islands that undergo methylation in pancreatic cancers are normally methylated in the adjacent duodenum. Because duodenal methylation patterns are an important consideration when sampling pancreatic tissues for pancreatic cancer methylation alterations, we determined the DNA methylation patterns of 24 genes in the normal duodenum of patients with pancreatic disease and related these patterns to demographic factors. EXPERIMENTAL DESIGN: The nonneoplastic duodenal mucosa of 158 patients with pancreatic carcinoma and 41 patients with chronic pancreatitis was analyzed using methylation-specific PCR and combined bisulfite restriction analysis. Secretin-stimulated pancreatic/duodenal juice from 15 individuals undergoing endoscopic investigation for upper gastrointestinal disease was also analyzed. RESULTS: Low-level methylation was detectable by methylation-specific PCR in the nonneoplastic duodenum of many patients with pancreatic cancer and chronic pancreatitis as well as in the pancreaticoduodenal secretions of patients without pancreaticobiliary disease. For many genes, the prevalence of methylation increased with age and was more prevalent in patients with pancreatic cancer than in age-matched patients with chronic pancreatitis. Our results indicate that strategies to detect pancreatic cancer using aberrantly methylated genes should rely on analysis of pure pancreatic juice rather than on pancreatic juice collected within the duodenal lumen. Patients who develop pancreatic cancer may have a greater propensity to methylate CpG islands than age-matched controls.

Adult↗

Traumatic intramural hematoma of the duodenum: report of one case.

Traumatic intramural hematoma of the duodenum is a rare entity in children. A high clinical index of suspicion is necessary for early and accurate diagnosis. We report a 10-year-old girl presenting with a sudden onset of epigastralgia after impact on the epigastrium by the edge of a wooden bed while playing on it. Symptoms of high gastrointestinal obstruction were severe, with bilious and large amounts of nasogastric effluent. Image studies revealed a large hematoma extending from the second to the third portion of the duodenum. Her condition was managed successfully by conservative treatments with nasogastric decompression and total parenteral nutrition. Her hospital course was uneventful. This report serves as a reminder to clinicians to consider intramural hematoma of the duodenum in the differential diagnosis of blunt abdominal trauma.

Abdomen, Acute↗

[Simultaneous liver-pancreas-duodenum transplantation: one case report and review of the literature].

OBJECTIVE: To investigate the effect of treatment on end-stage liver disease and type-I diabetes mellitus with simultaneous liver-pancreas-duodenum transplantation. METHOD: In September 2003, one patient with chronic hepatitis B, liver cirrhosis, hepatic cellular cancer, and insulin-dependent diabetes received simultaneous orthotopic liver and heterotopic pancreas-duodenum transplantation. Liver and pancreas graft function was monitored after transplantation. RESULTS: The function of pancreas allograft was recovered immediately and the patient became insulin-independence postoperatively. The liver allograft was experienced an acute rejection episode and reversed by intravenous bolus methylprednisolone. The recipient was currently liver disease-free and insulin-free more than 21 months. CONCLUSIONS: The simultaneous liver-pancreas-duodenum transplantation is an effective method in the treatment of end-stage liver disease and type-I diabetes mellitus.

Diabetes Mellitus, Type 1↗

[The mechanisms of the stimulation of pancreatic secretion in drawing off its juice from the duodenum].

The effect of atropine on the pancreatic gland's secretion in conditions of partial or complete abduction of the gland's juice from the duodenum, was studied in dogs. Atropine inhibited the secretion if the latter was stimulated with hydrolysin (endogenous peptides) and exerted no such effect in stimulation of the secretion with exogenous peptides. Atropine inhibited the secretion in abduction of the secret from the duodenum. Administration of the secret into the duodenum abolished the atropine effect. Two mechanisms of the secretion inhibition seem to exist: M-cholinergic (low-threshold) and peptidergic (high-threshold) ones.

Animals↗

[Necrosis of the duodenum resulting from acute necrotising pancreatitis and treated with complete duodenopancreatectomy].

Necrosis of the duodenum resulting from acute necrotising pancreatitis is a rare but potentially lethal complication. A small number of cases has been reported so far, the majority of which having, unfortunately, had a lethal outcome. We present the cases of two patients, a 21-year-old woman and a 54-year-old man, both suffering from extensive duodenal necrosis stemming from acute necrotising pancreatitis, and both cases involving the second and third sections of the duodenum, one of which was probably caused by the thrombosis of nutritive blood vessels, the other by an abscess of the head of the pancreas. Due to the extent of the necrosis of the duodenum, there was no option to close so large a hole or to employ any less drastic procedure, so that a complete duodenopancreatectomy had to be performed. One patient survived and has remained in good health for a period of almost ten years, to date. The other patient died six days after surgery due to infection, in spite of an abscence of any sort of anastomic complications.

Adult↗

Comparison of an H2 receptor antagonist and a neutralizing antacid on postprandial acid delivery into the duodenum in patients with duodenal ulcer.

Measurement of the postprandial rate of acid delivery into the duodenum directly assessed the efficacy of two radically different acid-reducing therapies for duodenal ulcer disease. Cimetidine, 400 mg, with an ordinary solid meal decreased the 4-hr delivery of titratable acid and hydrogen ion into the duodenum by 63 and 86%, respectively (P less than 0.01 versus control). Liquid Maalox, 30 ml, 1 and 3 hr after an identical meal reduced 4-hr delivery of acid by 47 and 74%, respectively (P less than 0.01 versus control). During the study period, the H2 receptor antagonist effected a continuous reduction in gastric acidity and the delivery of acid into the duodenum. The liquid neutralizing antacid produced a more fluctuating decrease in these parameters. However, given in these dosages, the magnitude and duration of the acid-reducing effect were similar for both treatments.

Aluminum Hydroxide↗

[The localization of neurons innervating the upper portion of the duodenum in the dorsal motor nucleus of the vagus nerve].

In anesthetized cats, using method of the retrograde transport of the horseradish peroxidase and electrophysiological techniques, the dorsal motor nucleus neurons innervating upper portion of the duodenum were found. Maximal number of such neurons were found within 1.0 to 2.5 mm rostral to the obex. The effects of the neurons stimulation on the electrical activity of the duodenum smooth muscles were studied. Possible role of the dorsal motor nucleus neurons in realisation of gastro-duodenum motor reflexes is discussed.

Action Potentials↗

Duodenum-preserving resection of the head of the pancreas in treatment of chronic pancreatitis.

Twenty-four patients underwent duodenum-preserving resection of the head of the pancreas for chronic pancreatitis of the head. All patients were reassessed in our follow-up clinic. Comparison with partial duodenopancreatectomy showed that both procedures can alleviate the majority of symptoms. However, duodenum-preserving resection of the head of the pancreas has lower early and late postoperative complication rates. It facilitates occupational rehabilitation, does not alter the patients glucose metabolism, and leaves fewer patients symptomatic in long-term follow-up. A higher rate of recurrence of pancreatitis of the tail is not found. Duodenum-preserving resection of the head of the pancreas is thus considered to be an alternative to partial duodenopancreatectomy in treatment of severe chronic pancreatitis of the head.

Adult↗

[Diagnosis and treatment of traumatic injuries of the duodenum and pancreas: 21 cases].

Twenty one consecutive patients who sustained injuries to the duodenum or/and pancreas were admitted to our hospital over a ten year period. Sixteen blunt injuries and 5 penetrating injuries were encountered. Penetrating injuries were always suspected and treated by time; following blunt injury diagnostic delay was encountered in 7 patients and insufficient surgical procedure because of intraoperative misinterpretation in 2 patients. Most of the patients had associated intra-abdominal organ injuries. Adjuncts to diagnosis such as abdominal roentgenograms, serum amylase levels and gastroduodenography were not helpful. CT-Scan and ultrasound allowed to confirm the suspected diagnosis in 3 cases only. Intraoperative diagnosis was also challenging. Complete mobilization of the structures surrounding the duodenum and the pancreas to provide entire exposure was necessary. In 6 patients treated first in a peripheral hospital, diagnosis of the injury have been missed at first laparotomy and reoperation was necessary in all of them. Suture closure of the duodenum and drainage of the pancreatic region were the most common reparative technique used. More complicated procedures with pancreatic and/or duodenal resection were performed in 6 patients. Overall mortality in patients surviving more than 24 hours was 14% (suture line dehiscence after delayed operation and one death due to brain injury).

Adolescent↗

Muscarinic M1 receptors stimulate a nonadrenergic noncholinergic inhibitory pathway in the isolated rat duodenum.

We examined the effect of the muscarinic agonist McN-A-343 (4-m-chlorophenylcarbamoyloxy-2-butynyl trimethyl ammonium) on in vitro preparations of rat small intestine. McN-A-343 (0.1-10 microM) induced a concentration-dependent relaxation of duodenum, jejunum and ileum. This effect was due to activation of muscarinic receptors of the M1 subtype, inasmuch as it was antagonized by atropine (pA2, 8.93) and by the selective M1 antagonists pirenzepine and dicyclomine with high affinity (pA2, 8.09 and 8.14, respectively). Tetrodotoxin, but not hexamethonium, abolished McN-A-343 relaxation indicating involvement of neural pathways and absence of nicotinic transmission. Moreover, in the presence of apamin (0.1 microM) McN-A-343 induced a contractile response. Unlike McN-A-343, acetylcholine contracted the rat duodenum; its concentration-response curve was significantly potentiated by tetrodotoxin, suggesting stimulation of the McN-A-343 sensitive receptor by acetylcholine (P less than .01). Prior treatment of animals with reserpine reduced the potency of McN-A-343 only by 2.5-fold. The gamma-aminobutyric acid (GABA) antagonist bicuculline inhibited McN-A-343 with an affinity comparable to that found for GABA(A) receptors (pA2, 5.52), indicating involvement of GABAergic fibers. ATP is also likely to play a role, as desensitization experiments showed that alpha-beta-methylene ATP induced a comparable decrease of both McN-A-343 and its own response. In contrast, desensitization induced by GABA occurred to a minor extent. Thus, in the rat duodenum, activation of an M1 muscarinic receptor induces relaxation by releasing GABA, and possibly ATP, from myenteric neurons.

(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethy↗

["Wheeling-off" of the duodenum in gastric resection in hemorrhagic duodenal ulcer].

Truncal vagotomy associated with antrectomy is required in the treatment of terebrant and haemorrhagic ulcers of the first duodenum. The main problem lies in the closure of the duodenal stump. The "wheeling-off" procedure is a reliable technique which consist of separating the duodenum from the pancreas by carrying out dissection into the posterior wall of the duodenum, between its two muscular layers. Closure is obtained by means of a stappler. This procedure was performed in 23 patients without leakage or recurrent bleeding.

Adult↗

[Characteristics of the diagnosis and surgical treatment of injuries of the duodenum].

Specific features of diagnostics and surgical treatment of injuries of the duodenum in 41 patients with open and closed traumas of the abdomen are described. A wide access by means of mobilization of the right colic flexure should be used for the revision of the duodenum. In cases with closed injuries of the duodenum, in addition to maintenance of its reliable decompression, it is necessary to exclude the intestine from the passage of the gastric content. In order to prevent postoperative pancreatitis cholecystostoma should be made.

Duodenum↗

[Injury of the duodenum in closed abdominal trauma].

Analysis of 11 cases with injury to the duodenum showed that the presence of a hematoma, gelatinous pulmonary edema, and yellowish-green fatty tissue around the duodenum are evidence of the possible damage of its retroperitoneal part. Closure of the duodenal defect with double-row silk sutures and their peritonization with a free area of the parietal peritoneum is advisable. In retroperitoneal rupture of the duodenum the operation must be completed by drainage of the retroperitoneal space and decompression of the beginning of the small intestine with a nasogastric catheter. The possible complications are described. The Postoperative mortality was 36.3%.

Abdominal Injuries↗

[Analysis with immunoreactive CCKs and bioactive CCKs in rat duodenum and brain].

We extracted immunoreactive CCKs from rat duodenum and brain. We fractionated these samples by gel-filtration and analyzed with immunoreactivity and bioactivity of each fraction. 1) Elution profile of CCKs in duodenum and brain was well corresponding to that of bioactive CCKs. 2) Contents of CCKs determined by both RIA and Bioassay in each fraction were well correlated with each other. 3) Apparent molecular weights of CCKs of duodenum calibrated in column chromatography were approximately 2200, 1000 and 800. 4) Apparent molecular weight of CCK of brain seemed to be 1000.

Animals↗