Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Autolysis”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 451 records · Page 25Linked to original sources
PubMed1983

[Proton relaxation characteristics of human and animal tissue in vitro. Changes during autolysis and fixation].

The results of measurements of proton relaxation times of various tissues from rats, pigs and humans are reported; these were obtained by a resonance spectroscope at 20 MHz and 40 degrees C. There were specific differences in both relaxation times (T1 and T2) of the liver and spleen. There was a difference of more than 150 ms in the longitudinal relaxation time between grey and white cerebral tissue. Autolytic changes show an increase in both relaxation times. Fixation produced a reduction in T1 only. The significance of these findings for NMR tomography is discussed.

Animals↗
PubMed1996

Penicillin resistance and autolysis in enterococci.

Comparison of several cell wall-related properties of the ATCC 9790 strain and the R40 strain, a penicillin-resistant, PBP5 overproducing strain, and Rev14, a penicillin-hypersensitive, PBP5-deficient strain, is consistent with a role of the genetic element, psr, in the global regulation of lysozyme sensitivity, autolytic capacity, and wall-rhamnose-containing polysaccharide content. These parameters appear to be independently regulated by a system that involves psr in a currently unknown manner.

Autolysis↗
PubMed1978

Demonstration of the physiological role of autolysis by a comparative study with a wild-type and its non-autolytic mutant of Micrococcus lysodeikticus (luteus) cultivated with externally added proteolytic enzymes.

The log phase cells of autolytic Microccus lysodeikticus (luteus) IFO 3333 did not autolyze when grown in the presence of trypsin although the growth curve and morphology of the cells were not influenced. A non-autolytic mutant was obtained by subculture of the wild-type strain IFO 3333 on an agar slant containing 1% glucose. The mutant (strain MT) was wild-type IFO 3333 which occurred singly or in irregular masses. The mutant MT grown in a culture medium containing trypsin caused remarkable alteration in cell morphology: large cell packets consisting of a number of "unit tetrads" arranged regularly in three dimensions were formed by the addition of trypsin to the medium. The findings suggest that inhibition of the separation of divided cells is brought about by inactivation or suppression of a cell wall autolytic enzyme which plays an important role in the separation step and is accessible to externally added trypsin in the mutant cells but not in the wild-type cells. The possibility that there are two kinds or phases of autolytic enzymes "a physiological autolytic enzyme" and "a useless autolytic enzyme", is discussed.

Agglutination↗
PubMed1979

Glomerular immune deposits in kidneys from patients with no clinical or light microscopic evidence of glomerulonephritis. Assessment of the influence of autolysis on identification of immunoglobulins and complement.

Using a direct fluorescent staining technique, immunofluorescent microscopy (IFM) demonstrated glomerular deposits of IgG and IgM and/or fractions of complement in kidney tissue from 24% of 33 patients examined post mortem and in 39% of kidney biopsies obtained from 23 patients on lithium treatment. All the patients investigated had a normal blood pressure. There was no evidence of glomerulonephritis (GN) neither clinically, at light microscopy, nor on laboratory investigation. These "spontaneously" deposited immunoglobulins and complement fractions in glomeruli will obviously by demonstrated in kidney biopsies from patients with GN, even though they bear no relation to the disease. This will therefore preclude an immunopathological classification which relates to histological and clinical findings. A control study of the IFM findings in glomeruli on 13 surgically removed kidneys showed optimal identification and no further glomerular deposition of immunoglobulins during the 72 hours following nephrectomy, at temperatures below 10 degrees C. Clq and C3 were less stable and were only demonstrated with certainty up to 24 hours after nephrectomy.

Adult↗