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PubMed1963

NITROUS ACID REACTIVATION OF ULTRAVIOLET-IRRADIATED TRANSFORMING DNA FROM HEMOPHILUS INFLUENZAE.

Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating enzyme from bakers' yeast. The pretreatment with nitrous acid affords a slight protection for denatured C(25) DNA and Sm(250) DNA against ultraviolet irradiation, but this pretreatment sensitized the E(20) DNA to this irradiation.

DNA↗
PubMed1964

STREPTOMYCINOID ANTIBIOTICS: SYNERGISM BY PUROMYCIN.

Puromycin synergizes the lethal action of streptomycin and related antibiotics. This is interpreted to mean that puromycin action uncovers a sensitive site (or sites) on the 30S ribosome. The streptomycinoid antibiotics can then associate more readily with the ribosome and inhibit further synthesis of valid protein.

Anti-Bacterial Agents↗