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At least 433 records · Page 24Linked to original sources
PubMed2006

In vitro biofilm formation on the surface of resin-based dentine adhesives.

Prevention of root caries on exposed root surfaces in the aging population is a significant challenge. Bonding resins can be applied to exposed root surfaces as sealants; however, minimal data exists regarding biofilm formation on the surface of these resins. We hypothesized that an antibacterial dentine-bonding resin containing methacryloxydodecyl-pyridiniumbromide (MDPB) may reduce biofilm formation. Biofilms were produced in pooled stimulated natural whole saliva, supplemented with 1% sucrose, on the surface of 5 dentine-bonding resins (Clearfil SE, OptiBond Solo, Protect Bond, Protect Bond Primer, and Xeno III) using untreated root surfaces as controls. Biofilms were stained using the Live:Dead Baclight bacterial viability stain, viewed with confocal microscopy, and analyzed using ImageJ image-analysis software. Resin surfaces encouraged attachment of live bacteria compared with root surfaces. All resins showed similar bacterial colonization in sections adjacent to the resin surface, but in the central and outer portions of biofilms, Xeno III and Protect Bond Primer showed a viable bacterial load similar to that of the root surface. Fluoride-releasing resins (OptiBond Solo/Protect Bond) did not show reduced biofilm formation. Thus, antibacterial agents within the resins have a minimal effect on biofilm formation, particularly when directly adjacent to the root surface.

Anti-Bacterial Agents↗
PubMed2006

Connective tissue grafting on resin ionomer in localized gingival recession.

BACKGROUND: One of the main objectives of periodontal reconstructive surgery is the coverage of exposed roots that occur due to gingival recession. On some occasions, where a caries, root resorption, or amalgam restoration exists on the exposed root surface, the treatment planning becomes more complex. This case report describes the use of a subepithelial connective tissue graft (SCTG) on a resin ionomer-restored root surface to treat gingival recession that is complicated with the above-mentioned handicaps. METHODS: An amalgam restoration and carious lesion were removed following full-thickness flap reflection, and the cavity was restored with glass ionomer cement. An SCTG was placed onto the restoration, and the flap was coronally positioned. A porcelain crown restoration was performed 9 months after surgery. RESULTS: At 3-, 6-, and 9-month follow-ups, probing depths were reduced and gain in attachment level was obtained with no clinical signs of inflammation in gingiva. Monthly periodontal controls revealed that creeping attachment had occurred on the restoration during the follow-up periods. CONCLUSION: This single case report serves as a good example to show that SCTG can be successfully performed to treat gingival recession associated with a glass ionomer-restored root surface.

Adult↗
PubMed2004

[Curriculum reform in dental medicine at the University of Ghent].

The need for dental and oral treatment in the society is constantly changing. Epidemiological studies show that in the rapidly aging population in Western Europe, caries (except for root caries) is declining but more complex periodontal treatment is needed. The number of completely edentulous patients is decreasing. Patients have a longer life expectancy but are medically and psychologically more compromised. Many more patients are at high risk for medical complications. Therefore, a more medical orientation of the dental education is needed. The basic cellular and molecular knowledge in medicine is rapidly expanding. The practical application of this expanded knowledge has been introduced in dentistry such as use of DNA probes, genetic testing, vaccines etc. The graduating dentist should be aware of the scientific progress and be able to apply this technology in his future practice. Therefore, the urgent need was felt to reform the dental education fundamentally and to give it a more medical orientation. Teaching is organised in coherent blocks of lectures covering specific parts of' a discipline and discussing the content from different angles by different lecturers. Basic information (eg. physiology, microscopy, microbiology) is provided in the same block as the clinical and therapeutic information. Preclinical laboratories prepare the student for the clinical phase of a discipline and are not any longer devoted to dental technical laboratory work. More time is given to prosthetic planning, communication with the dental technician and to analyse the biological effects of prosthetic appliances. In the final year a large number of teaching hours is devoted to general medical pathology including physiopathology, dermatology, general head and neck pathology and surgery (ENT, oncology, orthognathic surgery) as well as gerodontology including general medical, psychological and nutritional themes. Finally, clinically the student has a multidisciplinary approach in his diagnosis and treatment of patients. The final aim is train and educated oral physicians.

Belgium↗
PubMedDate not supplied

Streptococcus mutans genotypes isolated from root and coronal caries.

The aim of the present study was to evaluate the colonization profile and clonal distribution of Streptococcus mutans isolated from oral cavities that presented coronal and root caries lesions. The isolation and biochemical identification of mutans streptococci were carried out by using saliva samples, dental plaque, and tissue from the caries lesions. In order to confirm their molecular identity, S. mutans and Streptococcus sobrinus were submitted to the PCR method, using specific primers for portions of the glucosyltransferase genes (gtfB and gtfI, respectively). The AP-PCR method was used to detect the genetic polymorphism of S. mutans strains. Among the isolated and identified species, S. mutans showed a significantly greater frequency of isolation (59.2%) than the other mutans streptococci. Each of the subjects harbored two to ten genotypes of S. mutans, randomly distributed in different sites. S. mutans genotypes showed no evidence of variability in colonizing noncarious and carious surfaces within the same individual, nor evidence of etiologic differences between coronal and root caries. This study showed that no particular genotype of S. mutans is uniquely associated with the initiation and progression of caries, and that root and coronal caries can emerge in the presence of a broad spectrum of S. mutans clones.

Aged↗
PubMed2003

Effect of smear layer on root demineralization adjacent to resin-modified glass ionomer.

The cariostatic effect of resin-modified glass ionomer (RMGI) on secondary root caries is well-documented. However, this beneficial effect may be dependent upon the mode of cavity surface treatment. To investigate this relationship, we studied 4 cavity surface treatments prior to the placement of RMGI: no treatment (None), polyacrylic acid (PAA), phosphoric acid (H(3)PO(4)), and Scotchbond Multi-Purpose adhesive (SMP) as a control. Specimens were aged for two weeks in synthetic saliva, thermocycled, and subjected to an artificial caries challenge (pH 4.4). Polarized light microscopy (PLM) and microradiography (MRG) showed significantly less demineralization with the H(3)PO(4) cavity surface treatment as revealed by ANOVA and Tukey's multiple comparisons (p < or = 0.05). Dentin fluoride profiles determined by electron probe microanalysis (EPMA) supported PLM and MRG findings. It may be concluded that removal of the smear layer with phosphoric acid provides significantly enhanced resistance to secondary root caries formation adjacent to RMGI restorations.

Acid Etching, Dental↗
PubMed2002

Penetration of an antibacterial dentine-bonding system into demineralized human root dentine in vitro.

In this study, the penetration of three proprietary dentine-bonding agents (Prime & Bond 2.1, Single Bond, Liner Bond 2) and experimental dentine-bonding systems incorporating an antibacterial monomer, 12-methacryloyloxydodecylpyridinium bromide (MDPB), into artificial root caries lesions was evaluated, and the bactericidal activity of each material against Streptococcus mutans or Lactobacillus casei impregnated into demineralized dentine blocks was assessed. All of the commercial dentine-bonding agents were capable of penetrating into the artificial carious lesions to more than 150 microm. The depth of penetration of the experimental systems, which were based on Liner Bond 2, was not significantly different from that of their parent product. Liner Bond 2 primer exhibited the greatest bactericidal effects among the three proprietary dentine-bonding agents tested. Bactericidal activities of experimental primers containing MDPB were greater than those of any other products, and the application of 4% MDPB-containing primer resulted in complete killing of bacteria in demineralized dentine. The results indicate that the penetration of dentine-bonding agents into extensively demineralized root dentine is possible in vitro, and the experimental dentine-bonding systems containing the antibacterial monomer MDPB are capable of killing bacteria within demineralized dentine. This could be of benefit when managing root caries lesions.

Acetone↗
PubMed1999

[A recent observation of clinical effects by combined application of lanthanum,cerium and fluoride-contained gel on root surface caries]

OBJECTIVE:The present study was to investigate the effects of combined application of lanthanum,cerium and fluoride-contained gel on root surface caries.METHODS:2000ppm lanthanum.cerium and fluoride-contained gels were used by various procedures to treat 164 teeth with root surface caries.RESULTS:The six months follow-up data revealed that combined procedure showed a better effect than each kind of trace element used alone.CONCLUSION:The study indicated that combined F/La(Ce) applying procedure has a potential value on the carious resisted root surface.

Journal Article↗