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At least 433 records · Page 24Linked to original sources
PubMed2006

Equilibrium, kinetics and mechanism modeling and simulation of basic and acid dyes sorption onto jute fiber carbon: Eosin yellow, malachite green and crystal violet single component systems.

Batch experiments were carried out for the sorption of eosin yellow, malachite green and crystal violet onto jute fiber carbon (JFC). The operating variables studied are the initial dye concentration, initial solution pH, adsorbent dosage and contact time. Experimental equilibrium data were fitted to Freundlich, Langmuir and Redlich-Peterson isotherm by non-linear regression method. Langmuir isotherm was found to be the optimum isotherm for eosin yellow/JFC system and Freundlich isotherm was found to be the optimum isotherm for malachite green/JFC and crystal violet/JFC system at equilibrium conditions. The sorption capacities of eosin yellow, malachite green and crystal violet onto JFC according to Langmuir isotherm were found to 31.49 mg/g, 136.58 mg/g, 27.99 mg/g, respectively. A single stage batch adsorber was designed for the adsorption of eosin yellow, malachite green and crystal violet onto JFC based on the optimum isotherm. A pseudo second order kinetic model well represented the kinetic uptake of dyes studied onto JFC. The pseudo second order kinetic model successfully simulated the kinetics of dye uptake process. The dye sorption process involves both surface and pore diffusion with predominance of surface diffusion at earlier stages. A Boyd plot confirms the external mass transfer as the rate limiting step in the dye sorption process. The influence of initial dye concentration on the dye sorption process was represented in the form of dimensionless mass transfer numbers (Sh/Sc(0.33)) and was found to be agreeing with the expression:

Adsorption↗
PubMedDate not supplied

Methylene blue dye as an alternative to isosulfan blue dye for sentinel lymph node localization.

Isosulfan blue dye has been used with increasing frequency in localizing sentinel lymph nodes in breast cancer patients. Few alternative types of dye have been investigated. In a prospective study of 30 patients, methylene blue dye was used instead of isosulfan blue dye to localize the sentinel lymph node. The methylene blue dye localization technique was successful in 90% of patients. These results are similar to those for isosulfan blue dye. This study describes methylene blue dye localization as a successful alternative to isosulfan dye in identifying the sentinel node in breast cancer patients. The methylene blue dye technique offers a substantial cost reduction.

Adult↗
PubMed2001

Lymphatic mapping in breast cancer: combination technique.

The current standard of care for patients with invasive breast cancer is complete removal of the tumor by mastectomy or lumpectomy, with documentation of negative margins, followed by complete axillary dissection. Our group has examined the efficacy of lymphatic mapping of the sentinel node via the combination technique in 594 patients with breast cancer. A radiocolloid was injected at six intraparenchymal locations surrounding the tumor 2 hours prior to the operation. A 1% solution of isosulfan blue dye was injected into the breast just before prepping the patient. The breast was compressed and massaged for 5 minutes. The surgical and pathological data were prospectively collected. Lymphatic mapping of the sentinel node with a combination of blue dye and radiocolloid enhanced preop localization, operation efficiency, internal mammary node detection, and quantitative measure of detection. The combined technique also significantly reduced the learning curve for achieving proficiency.

Breast Neoplasms↗
PubMed1985

[Comparative study of chemosensitivity tests of human urogenital tumors between a clonogenic assay and a novel dye exclusion test].

There is a need for chemosensitivity tests of human tumors to become simpler and to have a better correspondence with their therapeutical record. We examined and compared two chemosensitivity tests in vitro. One was a clonogenic assay performed using a double agar system and the other was a novel dye exclusion method that was a modification of the traditional dye exclusion method of Weisenthal. Cultured cells and surgical speciners of urogenital tumors were used for the present experiments. Comparison of chemosensitivity between the clonogenic assay and the novel dye exclusion test resulted in a similar dose response for each, and both tests were suitable not only for cultured cells but also surgical speciners. Among surgical speciners, 17 of 25 cases (68%) tested by clonogenic assay and 8 of 11 cases (73%) tested by novel dye exclusion test were successful in detecting drug sensitivity. Renal cell carcinomas had higher plating efficiency and showed a higher success rate in the clonogenic assay than any other urogenital tumors. On the other hand, the novel dye exclusion method depended on tumor cell growth in suspension culture. Among 11 cases on which both the clonogenic assay and the novel dye exclusion method were performed, only in one case was there failure to estimate drug sensitivity. Therefore, more successful results in examining the chemosensitivity of urogenital tumors might be expected by using both the clonogenic assay and the novel dye exclusion method.

Antineoplastic Agents↗
PubMed1995

Prolonged interference of blue dye "patent blue" with pulse oximetry readings.

Several blue dyes were reported to interfere with the accurate determination of oxygen saturation by pulse oximetry. However, in previous reports the interferences were observed only for a short duration. We have experienced a case in which the interference by a blue dye, "patent blue" continued for a long time. It was suggested that the blue dye, when it was injected intra-arterially and in an anaemic patient, interfered with the pulse oximetry measurement to a greater extent.

Catheterization, Peripheral↗
PubMed1975

The action of some triphenylmethane dyes on yeast and erythrocyte membranes.

The action of a series of triphenylmethane dyes on the membranes of two different cell types, yeast cells and erythrocytes, have been studied. The action of the dyes on yeast cells resembles the action of other positively charged bactericides. The sequence of the activity in the dye series is completely different for yeast cells and erythrocytes. Pararosaniline and crystal violet are much more active than malachite green and brilliant green on yeast cells, whereas the reverse sequence of activities applies in erythrocytes. The carbinol form of the dyes plays an important role as regards dye interaction with erythrocytes. Transition of the dye into the carbinol form is in water extremely slow, but is greatly accelerated in the presence of an organic phase, at least for malachite green and brilliant green, but not for crystal violet and pararosaniline. This explains the different action of the two categories of dyes on erythrocytes. Experiments with pure carbinol also support the hypothesis.

Animals↗
PubMedDate not supplied

Microleakage and the class V composite cavosurface.

The following conclusions may be made from this study: Substantial leakage occurs around unetched composite restorations. No procedure employed in this study consistently eliminated all leakage. Etching the peripheral enamel decreased penetration of the dye. The use of an intermediary bonding resin did not enhance marginal sealing over that obtained when only the etchant was used. The gingival margin was less resistant to penetration of the dye than the occlusal margin. Beveling the cavosurface margin produced a significant decrease in leakage, when employed with acid etching in Group II.

Acid Etching, Dental↗
PubMedDate not supplied

Different sites and modes of tracer injection for mapping the sentinel lymph node in patients with breast cancer.

Several studies have been published describing the techniques of identification of the "sentinel lymph node" (SN). There are marked differences in the techniques used by different investigators. Although agreement exists about the tracer particle size and the volume of injection, it is unknown what is the best site where to inject the tracer or the vital dye. The aim of the present study was to define the influence of different sites of injection on imaging of the lymphatic ducts and their SNs. We performed a pilot study in 30 consecutive patients with breast cancer who underwent SN biopsy by means of radioguided surgery and vital blue dye mapping. The patients were divided into six groups of five patients each; each patient was given a subdermal (ID) or peritumoral (IP) injection of radiotracer (300 microCi in 150 mL of 99mTc-HSA microcolloids; Albures, Amersham Sorin) above the tumor site in order to localize the SN. After the identification of the SN, a second injection of radiotracer was performed, which was different in each patient subset. In some cases more than one lymph node appeared on the lymphoscintigraphic scans after the second injection of radiotracer. When the peritumoral route was used it took longer to visualize the lymphatic pathways. For the ID route, injection at the exact skin projection over the tumor is optimal. Internal mammary lymph nodes were identified by both IP (2) and ID (1) injection, irrespective of the quadrant in which the tracer was injected. Our findings support the hypothesis of a precise topographic correspondence between the primary tumor and its specific SN. The subdermal route is more accurate than the intraparenchymal route, as it allows faster identification of the lymphatic vessels and SN. We believe these observations should be taken into account for the proper selection of the injection site of either vital dye or radiopharmaceuticals.

Adult↗
PubMed1999

[Preoperative angiographic landmarks of the gluteal muscle regions using Patent Blue V].

Use of a gluteus musculocutaneous flap is the most reliable technique for surgical repair of sacral ulcers. Surgery could be ideally performed when the flap is designed using only the upper or the lower half of the glutens maximus muscle depending on the superior gluteal artery or the inferior gluteal artery, respectively. The authors have developed a technique for preoperative demonstration of the vascular supply to both muscle and overlying skin from a single gluteal artery using superselective catheterization and arterial administration of a dye (Patent Blue V). This technique facilitates the surgical procedure but assessment of its usefulness will require further evaluation.

Angiography↗
PubMed1990

The influence of embedding on the stoichiometry of the pararosaniline-Feulgen stain in histological material.

In the present study the influence of the embedding technique on the dye-substrate-interaction of the Feulgen reagent has been investigated. Pieces of rat liver and of human lung tumours were fixed in formaline, Carnoy's or Bouin's solution or in SuSa and embedded in paraffin (P) or in glycolmethacrylate (GMA). Sections were stained with the pararosaniline-Feulgen-reagent. Mean optical density (MOD) of cell nuclei was measured with an image analyzer. GMA required longer times for hydrolysis and staining than P. the plateau phase of acid hydrolysis was longer in GMA. In general MOD was significantly lower in GMA than in P. The ratio of MOD of lymphocyte nuclei versus diploid basal cell nuclei in lung tissue was 0.97 in GMA and 0.88 in P, respectively. We presume that cross-linking of GMA monomers during polymerization stabilizes hydrolysed DNA. Steric factors are responsible for the slow diffusion of the dye-molecule. The influence of the embedding medium on the stoichiometry of the Feulgen stain has to be carefully considered for statistical evaluation of DNA-histograms.

Animals↗
PubMed2005

Dynamic MR lymphangiography and carbon dye for sentinel lymph node detection: a solution for sentinel lymph node biopsy in mucosal head and neck cancer.

BACKGROUND: The practical application of sentinel lymph node biopsy in squamous cell carcinoma of the head and neck is restricted by the time sensitivity of blue dye and lack of spatial resolution and nonspecific node enhancement with radiocolloid. This study evaluates the use of magnetic resonance (MR) lymphangiography and carbon dye labeling to circumvent these limitations. METHODS: Gadomer/carbon dye mixture was injected into the tongue and stifle of adult swine (n = 4). MR lymphatic mapping was followed by intraoperative mapping with isosulfan blue dye. Sentinel lymph node biopsy and completion node dissection were performed 60 minutes after injection in four nodal basins and at 7 days after injection in eight. RESULTS: The technique was successful in all 12 nodal basins. The sentinel lymph nodes were stained black at the time of the immediate and delayed dissections. CONCLUSIONS: MR lymphangiography provides temporal and anatomic localization of the sentinel lymph node with a single investigation. Carbon dye is a sensitive and persistent visual marker of MRI-targeted sentinel lymph nodes.

Animals↗
PubMed1993

A Coomassie brilliant blue G-250-based colorimetric assay for measuring activity of calpain and other proteases.

The activity of cytosolic calcium-dependent neutral protease (calpain) is commonly measured using casein as a substrate. A novel, modified caseinolysis assay is now developed. It involves incubation of calpain with substrate casein, followed by removal of an aliquot to which Coomassie brilliant blue G-250 dye reagent is added. The assay is based on the observation that the dye interacts only with protein but not the proteolytic products (small peptides and amino acids). Unlike the existing caseinolysis assay, this novel assay does not require separation of substrate from products and measurement is done in the visible range (595 nm). It is also more sensitive than existing colorimetric assays which use dye-conjugated protein substrates. The assay can also be used to measure the activity of other proteases such as trypsin and papain.

Amino Acid Sequence↗
PubMed1980

Quantitative aspects of the cytochemical Feulgen-DNA procedure studied on model systems and cell nuclei.

Quantitative aspects of DNA losses during fixation and pararosaniline(SO2)-Feulgen staining of microscopic preparations were studied. The preparation of a new cytochemical model, consisting of DNA-protein layers (with thicknesses between 0.1 and 5.0 micrometer) on microscopic glass slides is described and potentialities and limitations of this model are discussed. Polyacrylamide films into which high molecular weight calf thymus DNA or chicken erythrocyte nuclei had been constrained served as another model. As biological objects chicken erythrocyte nuclei and rat liver nuclei either in suspension or on microscopical glass slides were used. The experimental results indicate a loss of about 5% of the DNA due to the fixation procedure applied. Hydrolysis in 5 N HCl at room temperature, staining with the pararosaniline-Schiff medium and rinsing with sulfurous acid induced losses of DNA too, varying in amount depending on the type of preparation used. About 10% of the original DNA content is lost in total from chicken erythrocyte nuclei and rat liver nuclei dried on microscopical glass slides, from chicken erythrocyte nuclei constrained in polyacrylamide films, and from DNA-protein layers on microscopic glass slides. For nuclei fixed and stained in suspension the total losses amount to about 40%. The differences in losses between various types of preparations are discussed. Biochemically, the content of DNA originally present per chicken erythrocyte nucleus was determined to be 2.52 pg, a value, which is in good accordance with reliable biochemical data published already. It is shown that calibration of cytochemical staining intensities into biochemical units or absolute amounts of material by use of a model system, is only reliable when it is known or to be expected that both the loss of material due to fixation and staining, and the stoichiometric relation between material present and dye molecules is identical. The same holds for the application of internal biological reference systems.

Animals↗
PubMed1988

Victoria blue B--a nuclear stain for cytology. A cytophotometric study.

The aim of this study was to investigate the staining characteristics of Victoria Blue B in alcohol solutions. Cytological specimens (liver and spleen tissue imprints, blood smears) were stained with methanol solutions of commercially available Victoria Blue B-Cl and with pure Victoria Blue B-BF4. The dye concentration, staining time, and protone concentration of the dye solution were varied. The dye solutions were characterized using spectrophotometry and thin-layer chromatography. Cytophotometry and image analysis were used to quantitate the staining pattern of cell nuclei. Feulgen-stained slides were used as controls. Victoria Blue B-BF4 gave excellent nuclear staining exhibiting a quantitative dye-substrate relationship, whereas commercial dyes resulted in lower staining intensity and less distinct nuclear texture. Dye concentration and staining time were, over wide ranges, not of critical importance for the quality of the staining. Under certain staining conditions, only cell nuclei were stained, with the background remaining completely unstained. We presume that, in alcohol solutions, Victoria Blue dye binds as a neutral dye molecule in conjunction with its anion. Victoria Blue B-BF4 staining provides a simple and reproducible staining technique for cytology which is suitable for use in automated cell-pattern recognition.

Animals↗
PubMed2006

[Intraoperative labeling of sentinel lymph nodes with a combination of vital dye and radionuclide tracer--results in sentinel lymph node-positive patients].

BACKGROUND: Sentinel lymph node biopsy enhances the accuracy of tumor staging in patients with malignant melanoma and can help select candidates for regional lymphadenectomy. There are two techniques for identifying the sentinel lymph node: intradermal injection of a radionuclide tracer or of a blue dye. We evaluated both methods to determine how they can be best utilized to locate a sentinel lymph node. PATIENTS AND METHODS: In a retrospective study, 323 patients with melanoma (tumor thickness > or = 0.75 mm) who underwent sentinel lymph node biopsy after both radionuclide and blue dye injection were evaluated. The labeling of lymph nodes showing micrometastasis by histopathological examination was determined. RESULTS: 63 patients showed sentinel lymph nodes with micrometastasis. All of these nodes (100 %) were labeled with radionuclide tracer, but only 90 % with blue dye. In 5 patients, only radionuclide labeling identified the histopathologically-positive lymph node. In 36 patients, several sentinel lymph nodes were identified, with the histopathologically-positive nodes usually showing a higher radioactive signal intensity than the negative ones. CONCLUSION: Since in some patients histopathologically-positive lymph nodes are only labeled by radionuclide tracer, radionuclide labeling is indispensable for locating sentinel lymph nodes. In contrast, labeling with blue dye represents a supplementary method, which can simplify the recognition of the sentinel lymph node during surgery.

Female↗
PubMed1997

Reduction of malachite green to leucomalachite green by intestinal bacteria.

Intestinal microfloras from human, rat, mouse, and monkey fecal samples and 14 pure cultures of anaerobic bacteria representative of those found in the human gastrointestinal tract metabolized the triphenylmethane dye malachite green to leucomalachite green. The reduction of malachite green to the leuco derivative suggests that intestinal microflora could play an important role in the metabolic activation of the triphenylmethane dye to a potential carcinogen.

Aniline Compounds↗
PubMed2005

Is blue dye indicated for sentinel lymph node biopsy in breast cancer patients with a positive lymphoscintigram?

BACKGROUND: The use of isosulfan blue dye in sentinel node biopsy for breast cancer has been questioned because of its risk of allergic reaction. We hypothesized that blue dye could be safely omitted in the subgroup of patients who have evidence of successful sentinel node localization by lymphoscintigraphy. METHODS: A retrospective review of patients with breast cancer and sentinel node biopsy was conducted. Information was collected on lymphoscintigraphy results, use of blue dye, and intraoperative and pathologic findings of sentinel nodes. RESULTS: We identified 475 patients with breast cancer who underwent 478 sentinel node biopsies. Both dye and isotope were given in 418 cases, of which 380 had a positive lymphoscintigram. In 5 of the 380 cases with a positive lymphoscintigram, the sentinel nodes obtained were blue but not hot, for a 1.3% marginal benefit of dye in the technical success of the procedure. Sentinel nodes positive for metastasis were found in 102 of 380 cases; in 3 cases, the only positive sentinel node was blue but not hot. Omission of the blue dye tracer would have increased the false-negative rate of the sentinel node procedure by approximately 2.5%. CONCLUSIONS: Even in sentinel node biopsy cases with a positive lymphoscintigram, the use of blue dye is beneficial for both improving the technical success of the procedure and reducing the false-negative rate of the procedure. Because the marginal benefits of dye justify its routine use, strategies to minimize the toxicity of blue dye are warranted.

Axilla↗
PubMed1999

Effect of self-association and protein binding on the photochemical reactivity of triarylmethanes. Implications of noncovalent interactions on the competition between photosensitization mechanisms type I and type II.

We have explored the photochemical behavior of cationic triarylmethane dye monomers and dimers free in solution and noncovalently bound to bovine serum albumin (BSA) and examined how self-association and the formation of host-guest complexes involving biopolymers and photosensitizers affect the competition between the photosensitization type I and type II mechanisms. Our results have clearly indicated that tri-para-substituted triarylmethane dyes bind efficiently to albumin as monomers and dimers and, interestingly, that the formation of dye aggregates in aqueous solutions is actually assisted by the protein. Protein-assisted dye aggregation takes place under conditions of high biopolymer loading (high [dye]/[protein] ratios), as attested by the appearance of a hypsochromically shifted absorption band (H-band) that overlaps with the spectral shoulder of the respective dye monomer. As predicted by the molecular exciton theory, the intersystem crossing efficiency in H-type dimers is expected to be higher than in the respective dye monomers, and photoinduced electron transfer events are intrinsically favored in dye aggregates as a result of the physical contact between donor and acceptor. We have found that when triarylmethanes are noncovalently bound to BSA their photoreactivity undergoes a remarkable enhancement, and that the photooxidation mechanism type I is particularly favored in the macromolecular environment. A comparative examination of the behavior of triarylmethane dyes with that of methylene blue have shown that in the case of methylene blue the binding phenomenon also favor the type I mechanism.

Animals↗