The inactivation of penicillopepsin with 1,2-epoxy-3-(p-nitrophenoxy) propane, an active-site directed reagent.
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1. Biochemical changes in male, Wistar rats, treated with different doses of 1,2-dichloropropane (50-500 mg kg-1 body weight), were investigated at the end of a 4-week treatment and after a 4-week recovery period. 2. The behaviour of Phase I and Phase II metabolic steps and of the angiotensin converting enzyme activity of the renal proximal tubule brush border were determined. 3. Phase II is more affected by the solvent than Phase I metabolism, and liver metabolism is more affected than the kidney. 4. Angiotensin converting enzyme activity from the proximal tubule brush border appears to be the most sensitive parameter of kidney involvement during treatment. 5. After a 4-week recovery period all the metabolic indices together with angiotensin converting enzyme activity have returned to normal. Only liver reduced glutathione content shows a slight, but significant, increase for the highest dose (500 mg kg-1 body weight). 6. The results show that the biochemical changes induced in liver and kidney by 1,2-dichloropropane are reversible.
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In 1998, the Iowa Department of Public Health (IDPH) and Iowa State University (ISU) Extension Department, with the assistance of local health departments, investigated a series of carbon monoxide (CO) poisonings associated with the use of liquified petroleum gas (LPG)-powered forklifts in light industry. In each episode, forklifts emitting high CO concentration levels were operated in inadequately ventilated warehouse and production facilities, which resulted in high CO accumulations. Employees at each site developed symptoms of CO poisoning, and some employees received inadequate or inappropriate medical care. This report summarizes the investigations and provides recommendations to prevent such incidents.
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