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Applying a most probable number method for enumerating planktonic, dissimilatory, ammonium-producing, nitrate-reducing bacteria in oil field waters.

A most probable number (MPN) method was used to enumerate dissimilatory ammonium-producing, nitrate-reducing bacteria (DAP-NRB) in oil field waters and to determine whether they were stimulated by nitrate addition used to control hydrogen sulfide production. An ammonium production medium with 5 carbon and energy sources (acetate, glucose, glycerol, pyruvate, and succinate) and nitrate was used in a 3-tube MPN procedure to enumerate DAP-NRB. These bacteria were detected in 12 of 18 oil field water samples, but they were seldom detected in wellhead samples. Three oil field water samples were amended with nitrate in serum bottles and the numbers of different NRB were determined over a 38-day incubation time. This amendment stimulated increases in the numbers of heterotrophic NRB and autotrophic nitrate-reducing, sulfide-oxidizing bacteria, but DAP-NRB remained a minor portion of these communities. Overall, DAP-NRB were present in many of the oil field waters that were examined but their numbers were low. It appears that DAP-NRB would play a minor role in the consumption of nitrate injected into oil field waters for the control of hydrogen sulfide production.

Bacteria↗

Warmer winters: are planktonic algal populations in Sweden's largest lakes affected?

Winters in Sweden have become warmer in the 1990s, and as a consequence the timing of ice break-up and the growth and decline of spring phytoplankton has shifted, starting earlier. Even spring temperatures have become warmer, leading to an earlier beginning of the summer phytoplankton growth. The spring-ward shift in phytoplankton population growth has resulted in an extension of the growing season by at least one month. Although mean total phytoplankton biomass from May to October has not increased, the spring and early summer biomass of temperature-sensitive phytoplankton groups, such as cyanobacteria and chlorophytes, has increased in the 1990s. No increase was noted for other phytoplankton groups. Considering that some species of cyanobacteria that commonly occur during a summer bloom, such as Anabaena, Aphanizomenon, and Microcystis, can be toxic, the effect of warmer winters on aquatic ecosystems is potentially far-reaching.

Animals↗

[Enumeration of active cells in bacterial plankton of the Rybinsk reservoir with 5-cyano-2,3-ditolyltetrasolium: comparison with other methods].

The enumeration of actively respiring bacterial cells in different biotopes of the littoral zone of the Rybinsk Reservoir during the spring period of ice thaw using the fluorescent dye 5-cyano-2,3-ditolyl tetrazolium chloride showed that bacterial communities growing on the bottom surface of the ice cover and in water overgrown by higher aquatic plants were most active. The number of active cells among individual bacterial cells averaged 20% and reached about 40% among aggregated and filamentous bacterial cells. The results of the count of active bacteria by this method were compared with those obtained by other methods.

Animals↗