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Results for “Optical Rotatory Dispersion”

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At least 433 records · Page 24Linked to original sources
PubMed2004

Non-invasive polarimetric measurement of glucose concentration in the anterior chamber of the eye.

BACKGROUND: Diabetes mellitus is one of the most common diseases in industrialized countries as well as in emerging economies such as India or China. One of the key technologies for diabetes therapy is semi-continuous monitoring of the glucose level of diabetics. METHODS: Compared with skin-perforating techniques, optical measurement techniques promising good results bear the potential for high patient compliance with more frequent measurements. Due to its excellent optical properties, the anterior chamber and the aqueous humor (AH) contained therein offer promise for non-invasive in vivo glucose measurements. However, a number of strongly limiting factors, such as the precise optical properties of the eye, laser safety regulations and subconscious eye movements during the measurement period have to be considered for in vivo applications. RESULTS: This article presents a high-resolution polarimetric measurement system that utilizes the optical rotatory dispersion (optical activity) of the glucose molecule for measurements of the glucose concentration in AH. CONCLUSION: Based on this example of a suitable optical measurement system, the special limitations and conditions that have to be considered for in vivo glucose measurement at the human eye are presented and analyzed. This includes the optical properties of the cornea and the anterior chamber, the impact of typical eye movements during a measurement and laser safety regulations.

Aqueous Humor↗
PubMed1971

Optical activity of membrane suspensions: calculation of artifacts by Mie scattering theory.

The circular dichroism, optical rotatory dispersion, and optical density of a suspension of erythrocyte ghosts are calculated from the measured optical properties of solubilized ghosts by classical general scattering theory (Mie theory). The ghost is represented by a solvent-filled spherical shell 7 nm (70 A) thick and 3.5 mum in radius. The 3- to 5-nm red shifts and unusual band shapes observed in the circular dichroism and optical rotary dispersion of suspensions of the intact ghosts, but not in the solubilized membranes, are reproduced by these calculations. Both differential absorption and differential scattering of left-and right-circularly polarized light contribute significantly to the calculated circular dichroism spectra. The artifacts of small membrane vesicles are shown to be less than those of intact ghosts. It is concluded that the characteristic anomalies in the optical activity of membrane suspensions are artifactual.

Cell Membrane↗
PubMed1969

Approximation of rotational strengths from molar rotation data and generation of rotatory dispersion curves for d-camphor-10-sulfonate.

Starting with the expression for optical rotatory dispersion in the absorption region that was arrived at by Condon, two series were considered for the purpose of achieving the experimentally observed, steeper wavelength dependence in the absorption region while retaining the established 1/lambda(2) law in regions removed from absorption. The first two terms of one series in which the second term exhibits a 1/lambda(6) wavelength dependence were found to calculate satisfactorily the optical rotatory dispersion curve of d-camphor-10-sulfonate from 400 mmu to 190 mmu when only three bands were considered. Evaluated at the extrema, the two-term expression can be approximated by a simple equation which allows calculation of the rotational strength of a nonoverlapping band by using only the wavelength and molar rotation of the extrema and the index of refraction of the solution. The rotational strengths calculated from optical rotatory dispersion data in this manner closely agree with those calculated from corresponding circular dichroism data. Thus when position and magnitude of rotatory dispersion extrema alone are reported for carbonyls, it is suggested that such published data may be converted to approximate rotational strengths.

Camphor↗
PubMed2005

Optical rotatory-dispersion-type spatial light modulator and characteristics of the modulated light.

Among known temporal-spatial light modulation methods, there is no realistic method that can precisely control a light pulse simultaneously in the temporal and spatial domains. By careful consideration of the symmetries and topological properties of electromagnetic waves, a novel spatial light modulator has been developed to create different far-field patterns for each wavelength of linearly polarized light composed of various wavelength components. The system consists of an optical rotatory dispersion device, which is like a Faraday rotator, and a spatial light modulator with parallel-alignment nematic liquid-crystal cells. Numerical simulation results show the effectiveness of this new spatial light modulation method.

Journal Article↗
PubMed1972

Studies of the denaturation and partial renaturation of ovalbumin.

1. The denaturation of ovalbumin by the reagents sodium dodecyl sulphate and guanidinium chloride was investigated, by following the changes in sedimentation velocity, optical rotatory dispersion and viscosity as a function of denaturant concentration. 2. With sodium dodecyl sulphate both the optical-rotatory-dispersion parameters a(0) and b(0) become more negative, the sedimentation coefficient decreases and the viscosity increases; significant differences in the denaturation profiles are observed. The change in each parameter is indicative of only limited denaturation. 3. With guanidinium chloride the transition occurs over the concentration range 1-4m: more extensive changes occur in all the physical parameters than with sodium dodecyl sulphate. The values of a(0) and b(0) are indicative of complete denaturation. Reduction by mercaptoethanol produces only minor further changes. 4. Renaturation was attempted from both denaturants, the removal of reagent being accomplished reversibly by controlled slow dialysis. Partial renaturation was observed, but aggregated or insoluble material was produced in both cases at relatively low concentrations of denaturant. Similar behaviour was observed with fully reduced protein in guanidinium chloride-mercaptoethanol; complete renaturation could not be brought about even at very low protein concentrations.

Dialysis↗