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PubMed1988

The determination of urinary 3-trans-hydroxyproline (3 OHP). I. Normal values in school children and adults.

3-trans-hydroxyproline is a hydroxyproline isomer present in collagens. It is more abundant in basement membrane collagen (collagen type IV) where it can be found in a relation of 12 residues per 1000 amino acid residues. This implicates its probable use as a marker substance for collagen type IV metabolism. Up to now only hydrolyzed urine samples were examined for the presence of this amino acid, thus indicating total urinary excretion. We are reporting the free urinary 3 OHP content of adults and school children. School children (age group 8-18 years) showed mean excretion of 1.066 micrograms/mg creatinine (SD +/- 0.376), SEM 0.054, min. 0.516; max. 2.397, range: 1.881. Adults (age 19-29 years) showed mean excretion of 0.618 micrograms/ml creatinine (SD +/- 0.465), SEM 0.134, min. 0.055; max. 1.634, range: 1.579. As given by the statistical calculation of unpaired t-test, adults showed significantly lower urinary 3 OHP levels: t = 3.518 and p = less than 0.0005. This can be explained by increased collagen metabolism of the growing organism and is in congruence with data reported in literature for total urinary 3 OHP excretion.

Adolescent↗
PubMed1987

Impact of smoking on the concentration and activity of alpha-1-antitrypsin in serum in relation to the urinary excretion of hydroxyproline.

The impact of active and passive smoking on the serum levels of alpha 1-AT, the trypsin inhibitory capacity (TIC), the trypsin inhibitory activity (TIA) and the urinary hydroxyproline to creatinine ratio (HOP-ratio) was studied. The subjects used in the study on active smoking were 167 healthy adult men and in the study on passive smoking 189 healthy primary school children. Serum levels of alpha 1-AT in active smokers were significantly higher than those in non-smokers. The TIC as well as the TIA in active smokers decreased with increasing number of cigarettes smoked. The urinary HOP-ratio increased significantly with increasing number of cigarettes smoked. On the other hand, in the case of passive smokers a significant difference was obtained only for the HOP-ratio. The correlations between all markers in active smokers were significant. Less significant correlations were found in the case of passive smokers. These results suggest that the urinary excretion of hydroxyproline can be considered as a marker for the imbalance between proteases and anti-proteases as a result of smoking.

Adult↗
PubMed1986

[Hydroxyproline in the rat lung following exposure to industrial ashes and surface dusts].

Experimental animals (female white rats of Wistar strain) were given intratracheally a suspension of soil dusts and electroenergetic ashes. 3 months later, hydroxyproline contents in lung tissue was determined with PROKOP'S and UNDENFRIEND'S method modified by TOMASZEWSKI and HANZLIK. The highest hydroxyproline levels were found in animals from experimental groups, which had been administered electroenergetic ashes.

Animals↗
PubMed1985

Preliminary studies of urinary hydroxyproline levels in rodents and in smokers.

Groups of rats were exposed to four dilutions of cigarette smoke over a period of our weeks. Compared to the control (untreated) group of animals, the 24-hour hydroxyproline output (relative to creatinine) was lower for all dilutions of smoke and showed a negative dose-response relationship. In human smoking studies, in which groups of 20 male subjects and 20 female subjects were switched between various cigarettes for periods of 2 weeks, hydroxyproline output (relative to creatinine) for each sex did not change with increasing nicotine uptake although the outputs for men and women were significantly different.

Adult↗
PubMed1979

[Urinary excretion of hydroxyproline during the healing period of bone fractures (author's transl)].

This article refers to the total hydroxyproline excretion during the healing period of bone fractures. The hydroxyprolyne excretion is twice as high in patients with fractures than in persons in good health. Its amount depends on the localisation of the fracture and the therapy chosen as well as the age of the patient. However, no significant therapeutic conclusions can be drawn from the quantitative analysis of hydroxyproline during the healing period of the bone fracture as far as the course of stabilization of the fracture is concerned.

Adolescent↗
PubMedDate not supplied

[Findings on total hydroxyproline excretion in patients with scoliosis].

Variations in the metabolism of collagen of patients with idiopathic scoliosis were studied by measuring the quantity of total hydroxyproline excreted in the urine in 24 hours. Results were correlated with the degree of deviation and the degree of rapid vertebral growth. From the results a hypothesis can be formulated that scoliosis is caused by altered metabolism of connective tissue with increased destruction of collagen demonstrated by the increased excretion of urinary hydroxyproline.

Adolescent↗
PubMed1979

[Hydroxyproline and acid glycosaminoglycans in acute cerebral circulatory disorders].

In patients with acute disorders of cerebral circulation dynamic examinations of hydroxyproline excretion, total content of glycosamineglycans (GAGs) and content of the latters' fractions were carried out. During the first week of the disease the total content of the GAGs in the urine daily portion was found to be increased, this increase being statistically significant and correlating with the size of the expected brain lesion. The fractional spectrum of the GAGs changed towards greater content of sulphonated fractions. By the 3d--5th week of the disease the hydroxyproline concentration in the urine sharply rose, an evidence of the intense organizational processes within the ischemic focus by that time.

Aged↗
PubMed1978

[Urinary hydroxyproline excretion in liver cirrhosis].

In 50 patients with a laparoscopically ascertained liver cirrhosis the hydroxyproline excretion was estimated in the 24-hour-urine. There could not be found any significant differences between hydroxyprolinuria in normal persons and in patients with liver cirrhosis. The increased hydroxyproline excretion is an unspecific symptom and thus cannot contribute to the diagnosis and the observation of the course of a liver cirrhosis.

Adult↗
PubMed1980

[Rarefying osteopathy in rheumatology. Methods of evaluation and comparison of urinary hydroxyproline and serum proline iminopeptidase].

Serum proline iminopeptidase and (where possible) urinary hydroxyproline were determined in 214 subjects: normal subjects, subjects with chronic kidney disease, subjects with chronic liver disease, subjects with osteitis deformans, subjects with rheumatoid arthritis, and subjects with osteoporosis. The values of the two parameters fully corresponded. It is suggested, therefore, that proline iminopeptidase can be determined instead of hydroxyproline in cases where a primarily destructive bone disease in present.

Adult↗
PubMed1982

Increased renal excretion of 3 hydroxyproline in patients with active glomerular nephropathies and with polycystic renal disease.

The renal excretion of 3 hydroxyproline (3 HYP) and 4 hydroxyproline (4 HYP) was investigated in control subjects and in patients with various renal diseases. In normal adult subjects urinary 3 HYP was 12.5 +/- 3.5 (SD) mumoles/24 hr, 4 HYP was 226 +/- 62 mumoles/24 hr and the percentage ratio 3 HYP/4 HYP 5.4 +/- 0.5. This ratio was reduced during growth because of a relative excess of 4 HYP. In patients with acute glomerular disease (n = 12) 3 HYP was increased to 17.1 +/- 5.8 mumoles/24 hr (P less than 0.01), and the ratio 3 HYP/4 HYP was 7.3 +/- 0.7% (P less than 0.01). Such an increase in 3 HYP was not observed in patients with chronic glomerulonephritis (n = 24) where 3 HYP was 9.6 +/- 5.0 mumoles/24 hr and 3 HYP/4 HYP 5.7 +/- 1.6% or with diabetic glomerulopathy (n = 6). In patients with chronic interstitial nephritis (n = 8) the 3 HYP/4 HYP ratio was decreased except in patients with polycystic renal disease (PKD) where it was increased (P less than 0.001). The daily urinary content of 3 HYP and 4 HYP was slightly altered by renal insufficiency. Urinary 3 HYP did not change significantly in patients with GN with the nephrotic syndrome whatever the histological lesion. These results indicate that urinary 3 HYP: 1) is increased when glomerulonephritis is clinically acute or subacute; 2) is increased in PKD whatever the level of renal insufficiency.

Acute Kidney Injury↗
PubMedDate not supplied

Hydroxyproline and zinc excretion in patients with neoplastic breast disease.

Hydroxyproline and zinc excretion was evaluated in 87 patients with neoplastic breast disease; 40 of these had carcinoma, without radiological and scintigraphy demonstration of bone metastases. In the group of patients with breast cancer, the mean values of hydroxyproline and zinc excretion were found to be significantly higher than in the group with benign breast disease; only 12 of the patients with histologically detected breast cancer had hydroxyprolinuria levels which were higher than the upper limit of the values obtained in the control group. The authors, therefore, conclude that the patients in this group must be considered as being at greater risk.

Adolescent↗
PubMedDate not supplied

The effect of varying oxygen tensions on hydroxyproline synthesis in mouse calvaria in vitro.

Six-day-old mouse calvaria were grown in vitro on a grid culture system in Medium 199 containing titriated proline. Gas atmospheres containing various oxygen concentrations up to 25% were introduced to influence the rate of collagen formation as determined by the synthesis of labelled hydroxyproline in the explants. There was an increase in synthesis in response to 15% oxygen with a possible further small increase in 25% oxygen. Measurement of the release of unlabelled hydroxyproline into the medium as an estimate of collagen breakdown indicates an increase in resorption with increasing oxygen concentrations up to 20%. In this model, therefore, there is increased collagen turnover with increasing oxygen tension in the physiologic range. Although the observations reflect collagen formation and do not necessarily measure bone formation, the results are consistent with data derived from other sources suggesting that bone formation is increased by improved oxygenation.

Animals↗
PubMed1994

[Effects of tanshionone to bleomycin induced pulmonary fibrosis of rats on histological changes and production of lipid peroxides and hydroxyproline].

Study was designed to explore the effect of sodium tanshionone IIA sulfonate on bleomycin-induced pulmonary fibrosis in rat. The amount of lipid peroxides (LPO), hydroxyproline in rat lung tissue homogenate was determined at 3, 7, 14, 28 day after administration respectively. The results suggest that the amount of LPO, hydroxyproline of rat lung homogenate were significantly decreased in sodium tanshionone IIA sulfonate group. In addition, histological changes and width of alveolar septa and percentage area of pulmonary fibrosis scars was measured. The results show that sodium tanshionone IIA sulfonate could ameliorate bleomycin induced pulmonary fibrosis in rat. The mechanism that sodium tanshionone IIA sulfonate could prevent and treat bleomycin-induced pulmonary fibrosis in rat was discussed.

Animals↗
PubMedDate not supplied

Serum hydroxyproline and hydroxylysine levels in patients with decompensated cirrhosis.

An increase in total (72.67-8.24 mumol/l) and free (14.14-1.97 mumol/l) hydroxyproline and total (7.16-0.72 mumol/l) and free (1.06-0.24 mumol/l) hydroxylysine was found in the serum of 30 patients with decompensated hepatic cirrhosis as compared to the values in 40 healthy subjects (48.70-5.89; 8.41-4.73; 4.34-2.71; 0.77-0.25 mumol/l, respectively). The results were not related to total serum protein concentration. The level of peptide-bound hydroxyproline and peptide-bound hydroxylysine was significantly higher in cirrhotic patients despite the decrease in serum protein content.

Adult↗
PubMed1994

Improved determination of small amounts of free hydroxyproline in biological fluids.

A fast, reliable, and sensitive (< 20 pmol) method for the quantification of 4-hydroxyproline is described. It ensures good separation of imino acid peaks, eliminates interference by primary amino acids, and guarantees full (96-105%) recovery of hydroxyproline (HYP). Interfering primary amino acids are derivatized by o-phthaldialdehyde and removed from the sample by use of a discardable C18 column. HYP is measured photometrically at 254 nm after a second derivatization with phenylisothiocyanate and isocratic separation on a reversed-phase HPLC column.

Chromatography, High Pressure Liquid↗
PubMed1993

Evaluation of new product for hydroxyproline determination in HPLC.

For the determination of hydroxyproline, HPLC methods are specific and sensitive, but expensive and time consuming. The aim of this paper was to evaluate a modification to the HPLC method, which employs a precolumn derivatization by DABSYL chloride. The modified method includes a further derivatization by orthophthalaldehyde (OPA), but it simplifies the chromatographic separation, saving time and reagents. Precision, recovery, linearity and accuracy for both methods have been evaluated. The linear regression analysis of the data has given the following correlation: Y = 1.04 X - 1.08 (r = 0.996). Moreover we checked that the preparation of the mobile phase for the modified method may not be strict. In fact, only two identifiable peaks are present: internal standard amino acid (1st peak) and hydroxyproline amino acid (2nd peak).

Chromatography, High Pressure Liquid↗
PubMedDate not supplied

[Calcium, phosphorus, hydroxyproline and nitrogen in inflammatory joint diseases].

The elimination of calcium, phosphorus, hydroxyproline and nitrogen was studied in 127 patients with inflammatory joint diseases and )6 healthy controls for 4 days. On the third day, 186 mg of calcium was administered intravenously. Provoked hypercalciuria tests were made in 35 males, 116 females with rheumatiod arthritis (RA), 18 males with ankylosing spondylitis (ASp), 8 postinfectious arthritis (PA) and 18 healthy controls (C). In 120 patients comparison was made between the ratios of eliminated P/hydroxyproline, Ca/hydroxyproline and P/Ca with regards to the results obtained in healthy controls. The kinetics of 47Ca were studied in 7 males with ASp and 4 C. The ratios Ca/P in serum and P/Ca in urine were studied in the same patients and compared with 21 C. The results show that the bone symptomatology of PA manifests itself by elimination of elevated amounts of all of the indicators studied, especially phosphorus. In RA there may be considerable oscillations of flow of urine due to the perspiration of patients. RA differs from decompensated coxarthrosis and gonarthrosis in that the patients eliminate significantly less calcium and phosphorus. Corticosteroids stimulate the elimination of hydroxyproline. Younger patients with RA (25-44) show changes compatible with osteoporosis, older females (45-64) display changes similar to those seen in osteomalacia, the oldest female patient (65-84) appear to have insufficient binding capacity for calcium. The hyposthesis is proposed that at the disease onset RA is characterized by an extremely marked syndrome of osteopathy. ASp is characterized by significantly reduced elimination of hydraxyproline, higher metabolic pool of calcium, lower elimination of calcium in urine and faeces and lower accretion to bone.

Age Factors↗