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An allometric study of hippocampal components. A comparative study of the brains of the European hedgehog (Erinaceus europaeus), the tree shrew (Tupaia glis), and the marmoset monkey (Callithrix jacchus).

Brains of western European hedgehogs, common tree shrews and common marmosets were perfused and stained according to Timm's method. The volumes of hippocampal components were determined with semiautomated quantitative techniques and compared by allometric methods. The results support previous findings that the size of the hippocampus in marmosets and tree shrews is about twice that of the hippocampus of the European hedgehogs of the same body weight. Three hippocampal substructures account for most of this progression. These are the hilus, the deep zone of the molecular layer of the area dentata, and CA1 (especially the cellular layer) of Ammon's horn.

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Natural antibodies to EBV-VCA antigens in common marmosets (Callithrix jacchus) and response after EBV inoculation.

Healthy common marmosets from two separate colonies, one in England and one in France, were found to have antibodies cross-reacting with Epstein-Barr virus structural antigens (EBV-VCA). All seropositive animals were at least 2 years old. Experimental EBV infection of marmosets of different ages led to seroconversion of inoculated weanlings. Adult animals either developed antibody for the first time or showed an increase in their existing titers. Both control and infected animals developed a progressive interstitial nephritis. The lymphocytic infiltration was more extensive, diffuse and immature in appearance in inoculated animals, but no definite evidence of lymphoproliferative disease or lymphoma was found.

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Body weight changes throughout pregnancy in the common marmoset Callithrix jacchus.

Adult female common marmosets were weighed weekly for periods of 6-30 months. Of 27 animals, 25 were pregnant at some stage of the investigation. Patterns of body weight change throughout singleton, twin and triplet pregnancies were obtained and compared. Maternal weight increase was dependent on the number of young in utero; however, little or no change in weight was observed during the first 13 weeks of gestation, irrespective of the number of offspring delivered subsequently. The overall maternal:fetal body weight ratio was 1.00:0.21, ranging from 1.00:0.11 for singleton to 1.00:0.26 for triplet pregnancies. Lactation had little or no effect on maternal body weight during the first 4 weeks postpartum.

Animals↗

Reproductive status of adult daughters in family groups of common marmosets (Callithrix jacchus jacchus).

Urine samples were collected from 6 pubertal female common marmosets at approximately 3-day intervals over a period of 3-7 months. All 6 females were the eldest daughters in their family groups. 3 of these females were subsequently removed from their families and sampled in isolation for another 2-3 months. Levels of urinary pregnanediol-3 alpha-glucuronide were measured by radioimmunoassay in order to establish whether these females showed ovarian cyclicity. No cyclical fluctuations in the levels of pregnanediol were observed in the females housed with their families even though the females were of a reproductively mature age. Cyclicity was quickly established (within 18-22 days) when the females were removed from the family. These results are discussed in the light of similar recent investigations on callitrichid reproduction and the behavioural implications are considered.

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Medial hypothalamic lesions and sexual receptivity in the female common marmoset (Callithrix jacchus).

Medial hypothalamic lesions cause major deficits in sexual receptivity in a variety of mammalian species. Effects of such lesions upon sexual receptivity in female primates have not been investigated. Two experiments are described in which thermal lesions were made in the hypothalamus in 11 ovariectomized or ovariectomized/adrenalectomized, oestradiol-treated common marmosets. Proceptivity (sexual initiating behaviour) was reduced or abolished by lesions in the dorsomedial or ventromedial hypothalamus. Sexual receptivity was unaffected except in 3 females in which damage to the ventromedial area was associated with a 25-42% increase in terminations of males' mounts. However, this effect was reversed by treating females with oestradiol. Therefore, medial hypothalamic lesions have pronounced effects upon proceptivity in the marmoset, whereas effects upon receptivity are much less pronounced or absent. These results are discussed in relation to the evolution of the hormonal control of receptivity in anthropoid primates as compared to other mammals.

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Changes in fatty acid composition of the cardiac phospholipids of the cotton-eared marmoset (Callithrix jacchus) after feeding different lipid supplements.

After feeding marmosets different lipid supplements for 6 months, the distribution of phospholipid classes and the fatty acid composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and diphosphatidylglycerol (DPG) were determined in their cardiac membranes. Supplementing the diet with linoleic-acid-rich sunflower seed oil raised the level of 18:2,n-6 in both PC and PE, but did not change the level significantly in DPG. When 18:2,n-6 was increased, the level of arachidonic acid (20:4,n-6) was significantly decreased in PC and PE. No arachidonic acid was present in DPG. Supplementing the diet with mutton fat did not markedly increase the level of saturated fats, nor did it markedly reduce the level of arachidonic acid in any phospholipid component. No dietary treatment altered the distribution of the major phospholipid classes.

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Ascorbic acid requirement and assessment of ascorbate status in the common marmoset (Callithrix jacchus).

Marmosets can tolerate an ascorbic acid (AA) deficiency for several weeks without clinical symptoms. After being fed an AA-free diet for 3 months, nonspecific deficiency symptoms became obvious. Different dietary levels of AA resulted in corresponding serum ascorbate levels. The kidney threshold of AA in marmosets is comparable to that in humans. When the minimal AA requirement is defined as the amount that is necessary to maintain a serum AA level above the kidney threshold, then about 20 mg AA/kg body weight is needed. This intake was achieved in our trial with a diet containing 500 ppm AA. Thus, the AA requirement of marmosets is severalfold higher than the AA requirement of humans.

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Plasma progesterone levels throughout the ovarian cycle of the common marmoset (Callithrix jacchus).

Radioimmunoassay of progesterone in marmoset plasma has been used to determine ovarian cycle length. Total cycle length was 30.1 +/- 3.8 days (mean +/- SD, n = 30, range 24-41 days, median 29.5 days). The pre-ovulatory (follicular) phase, during which progesterone levels were below 10 ng/ml, lasted for 8.8 +/- 3.7 days (mean +/- SD, n = 30, range 3-20 days, median 8.5 days). The post-ovulatory (luteal) phase, during which progesterone levels were greater than 10 ng/ml, lasted for 21.5 +/- 2.2 days (mean +/- SD, n = 30, range 14-29 days, median 21.5 days). Total cycle length was almost twice that recorded in an earlier study. The reasons for this difference are discussed.

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