Pathogenesis of Candida albicans infection following antibiotic therapy. I. The effect of antibiotics on the growth of Candida albicans.
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Production of a hyphal fringe around colonies grown on Pal's agar (sunflower seed agar) at 30 degrees C for 48 to 72 h provides a simple means of discriminating between isolates of C. dubliniensis and C. albicans with 100% accuracy. Of 128 C. dubliniensis isolates tested on this medium, all produced a hyphal fringe. In contrast, none of the 124 C. albicans isolates tested produced a hyphal fringe. Pal's medium has the added advantage of being prepared from inexpensive, readily available seeds.
The purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between C. dubliniensis and C. albicans. After 48 hours of incubation at 30 degrees C all 26 (100%) C. dubliniensis isolates tested produced chlamydospores on tomato juice agar as well as in the other three media evaluated. However, when we inoculated all media with C. albicans, the absence of chlamydospores became resulting in the following percents: tomato juice agar (92.47%), niger seed agar (96.7%), casein agar (91.39%), and sunflower seed agar (96.7%). These results indicate that tomato juice agar is another medium which can also be used in the first phenotypic differentiation between C. dubliniensis and C. albicans.
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The sensitivity to 5-fluorocytosine (5-FC), 5-fluorouracil (5-FU) and 5-fluorouridine (5-FUri) of 583 strains of C. albicans of human origin shows a relationship between their ecology, their serotype and their sensitivity to 5-FC. The incidence of the primary resistant strains isolated from patients with systemic candidiasis is of 4%, and 88.8% of the resistant strains belong to serotype B. In a closed community of premature infants the only resistant isolate amongst 78 strains recovered during hospitalization also belong to the B serotype and was introduced from outside. African strains of vaginal origin showed a higher incidence (21.6%) of resistance than did the european strains (4.5%) and this resistance was related to the B serotype predominant in the African environment. This raises the problem of the structure of the cell wall in connection with the active sites, the antigenic determinants and the enzymatic equipment.
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