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Results for “ROSANILINE DYES”

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At least 415 records · Page 23Linked to original sources
PubMed1999

Potential utility of sentinel node biopsy in the original surgical assessment of Hürthle cell tumors of the thyroid: 23-year institutional review of Hürthle cell neoplasms.

BACKGROUND AND OBJECTIVES: Great difficulty still exists in determining the potential malignancy of Hürthle cell tumors of the thyroid gland. Indications for the extent of resection vary greatly in the reported literature. Sentinel node biopsy has shown its usefulness as a prognostic indicator in both melanoma and breast cancer. The feasibility of using it as an integral part of Hürthle cell tumor surgery was investigated and is discussed. METHODS: Eleven patients diagnosed with Hürthle cell tumors between the years of 1975 and 1998 were reviewed. The last three patients had sentinel node biopsy with isosulfan blue dye as an integral part of their procedure. RESULTS: Sentinel node biopsy was accomplished without difficulty or complication in our last three patients. Two patients were considered to be benign by frozen section and final pathology. Their sentinel nodes were benign. One patient was considered malignant on both frozen and final pathology. His sentinel nodes as well as central node dissection revealed no lymphatic spread. CONCLUSIONS: Malignancy of Hürthle cell tumors of the thyroid is difficult to determine even on final pathological examination. The addition of nodal sampling may add valuable prognostic information. Sentinel node biopsy with isosulfan blue dye, although not previously reported for these tumors, appears to be a logical next step in the evolution of surgical management.

Adenoma, Oxyphilic↗
PubMed2001

Viability and infectivity of oocysts recovered from clams, Ruditapes philippinarum, experimentally contaminated with Cryptosporidium parvum.

This study confirms the important role of marine bivalve molluscs, destined for human consumption, as transmitters of cryptosporidiosis, zoonotic diarrhoeal disease caused by Cryptosporidium parvum. C. parvum oocysts recovered from seawater clams (Ruditapes philippinarum) were viable and infective in five of eight infected neonatal CD-1 Swiss mice. Oocysts were observed in clam gill and gastrointestinal tract tissue homogenates as well as in gill histological sections, by an immunofluorescent antibody technique. In vitro viability of recovered oocysts was also determined using fluorogenic vital dyes (75% viability).

Animals↗
PubMed2006

Bactericidal effect of malachite green and red laser on Actinobacillus actinomycetemcomitans.

The aim of this study was to investigate the ability of malachite green (MG) combined with a low-power red laser to kill Actinobacillus actinomycetemcomitans and to investigate MG photodegradation after photodynamic therapy (PDT) by optical absorption spectroscopy. The etiology of periodontal disease is that microorganisms form a bacterial biofilm on the surface of the teeth. It is an infectious disease and A. actinomycetemcomitans is considered an important agent in biofilm ecology. Instead of using antibiotics, PDT is an alternative approach to eradicate bacteria. Cultures of A. actinomycetemcomitans were exposed to a 30 mW diode red laser, in the presence or absence of MG. A group of cultures was treated in dark conditions in the presence of MG (0.01% w/v) for 5 min. In the presence of MG, two exposure times for laser irradiation were used: t=3 min (energy dose=5.4 J/cm(2)), and t=5 min (energy dose=9 J/cm(2)). The samples were diluted and bacterial colonies were counted and converted into colony forming units. Absorption spectra of the bacterial suspensions, MG, MG-stained bacterial suspensions, and photosensitized bacterial suspensions were obtained. A. actinomycetemcomitans can be photoinactivated by a red laser in the presence of MG. Significant differences were observed between the two energy doses used (p<0.05). Red laser alone and MG alone were not able to kill bacteria. Optical absorption showed that MG is photobleached after irradiation. These results indicate that A. actinomycetemcomitans can be photosensitized by red laser combined with MG and that the dye is photodegraded following irradiation.

Aggregatibacter actinomycetemcomitans↗
PubMed2005

Mechanism of TiO2-assisted photocatalytic degradation of dyes under visible irradiation: photoelectrocatalytic study by TiO2-film electrodes.

Photoelectrocatalytic degradation of various dyes under visible light irradiation with a TiO(2) nanoparticles electrode has been investigated to reveal the mechanism for TiO(2)-assisted photocatalytic degradation of dyes. The degradation of both cationic and anionic dyes at different biases, including the change in the degradation rate of the dyes and the photocurrent change with the bias potential, the degraded intermediates, the voltage-induced adsorption of dyes, the accumulation of electrons in the TiO(2) electrode, the effect of various additives such as benzoquinone (BQ) and N,N-dimethyl aniline (DMA), and the formation of active oxygen species such as O(2)(*-) and H(2)O(2) were examined by UV-visible spectroscopy, HPLC, TOC, and spin-trap ESR spectrometry. It was found that the dyes could controllably interact with the TiO(2) surface by external bias changes and charging of dyes. The cationic dyes such as RhB and MG underwent efficient mineralization at negative bias, but the N-dealkylation process predominated at positive bias under visible light irradiation. The discolorations of the anionic dyes SRB and AR could not be accelerated significantly at either negative or positive bias. At a negative bias of -0.6 V vs SCE, O(2)(*-) and dye(*+) were formed simultaneously at the electrode/electrolyte interface during degradation of cationic RhB. In the case of anionic dyes, however, it is impossible for the O(2)(*-) and dye cationic radical to coexist at the electrode/electrolyte surface. Experimental results imply both the superoxide anionic radical and the dye cationic radical are essential to the mineralization of the dyes under visible light-induced photocatalytic conditions.

Adsorption↗
PubMedDate not supplied

[Usefulness of lymphatic mapping with sub-areolar injection of patent blue dye, in the typification of breast cancer].

INTRODUCTION: Although the axillary radical dissection continues being the standard treatment for patient with breast cancer, the lymphatic mapping with biopsy of the sentinel node has arisen as a possibility to appropriately staging patients without palpable axillary nodes (NO) and to prevent axillary lymphadenectomy. The best method to perform it is with the association of radiocolloid and dye, nevertheless for this purpose an intraoperatively gamma-probe is needed, when is not possible to get it, the procedure must be done only with dye, therefore is important to select the most successful administration way for the sentinel node (SN) identification. OBJECTIVE: To show that the lymphatic mapping with subareolar injection of blue dye has a high success rate for lymphatic mapping in breast cancer patients. MATERIAL AND METHODS: Lymphatic mapping was practiced to 62 breast cancer patients with subareolar injection of blue dye. All the patients were subject to axillary dissection, the index of false negatives, sensitivity and negative predictive values were estimated. The need to perform the mapping with association of radiocolloid--dye was assessed according to the literature. RESULT: The SN was identified in 58 patients (93.5% success rate), the index of false negatives was of 5.2% with sensitivity of 93% and the negative predictive value was of 97.5%. CONCLUSIONS: The SN is a predictor of the axillary node state, the subareolar dye injection technique has a high success rate in the identification of the sentinel node.

Axilla↗
PubMed2001

Substituting Coomassie Brilliant Blue for bromophenol blue in two-dimensional electrophoresis buffers improves the resolution of focusing patterns.

In a new area of postgenomics challenges, the optimization of protein identification has become a central goal in microbiochemistry. In this work, we demonstrate that the substitution of Coomassie Brilliant Blue for bromophenol blue in two-dimensional electrophoresis (2-DE) buffers improves the focusing of whole proteins from Pseudomonas aeruginosa. This improvement of focusing concerns more particularly basic proteins. This enhancement may be attributed to a better transfer from the first to the second dimension, which probably highlights an increase in the solubility of proteins in the IPG strips. Hence, the use of an efficient tracking dye in the 2-DE buffers may enlarge protein recovery on proteome maps.

Bacterial Proteins↗
PubMed2001

Sentinel lymph node detection in patients with cervical cancer.

PURPOSE: We investigated the validity of sentinel lymph node (SLN) detection after radioactive isotope and/or blue dye injection in patients with cervical cancer. PATIENTS AND METHODS: Between December 1998 and May 2000, 50 patients (mean age 44 years) with cervical cancer FIGO stage I (n = 32), stage II (n = 16), or stage IV (n = 2) underwent SLN detection during primary operation (radical laparoscopic-vaginal or abdominal hysterectomy, exenteration). The day before surgery 1 ml of Albu-Res labeled with 50 MBq Technetium 99m was applied into the cervix at 3(00), 6(00), 9(00), and 12(00). Blue dye injection (Patentblue) occurred intraoperatively into the cervix at the same locations. RESULTS: The detection rate of SLN was 78%. Ten patients (20.0%) were diagnosed with lymph node metastases. No SLN was detected in 10 patients, of which 4 patients had positive lymph nodes. Sensitivity and negative predictive value were 83.3 and 97.1%, respectively. The false-negative rate was 16.6% (1 of 6 patients). After the combined injection, the detection rate, sensitivity, and negative predictive values were 100%. A mean of 2.7 pelvic and 2.6 para-aortic SLNs were detected. Para-aortic SLNs were located in the paracaval region in 66.6%, whereas pelvic SLNs were detected in 25.7% at the origin of the uterine artery and in 24.7% at the division of the common iliac artery. CONCLUSION: A combination of radioactively labeled albumin with blue dye allows successful detection of SLN in patients with cervical cancer. The clinical validity of this technique must be evaluated prospectively.

Adult↗
PubMed2005

Separation of synthetic food colourants in the mixed micellar system. Application to pharmaceutical analysis.

The paper presents a rapid method for the determination of commonly used synthetic food dyes by micellar electrokinetic capillary chromatography. Detection and separation conditions allowing complete resolution of 15 synthetic food colourants were investigated. The effect of different surfactants on the analytes mobility in relation to their structure was tested. After optimization procedure a dual micellar system was selected. All food dyes were separated in less then 20 min using a fused silica capillary in the borate/dodecylsulfate/deoxycholate buffer containing acetonitrile as organic modifier. The detection wavelength was set at 210nm. The method was successfully validated by determination of linearity ranges, detection limits, precision and repeatability for all colourants tested. In order to apply the method for pharmaceutical analysis a sample pretreatment procedures were found. Liquid pharmaceuticals were used as it or just after dilution with water. From tablets or capsules the colourants were isolated by adsorption on acidic aluminium oxide. The method was used for identification and if possible for quantification the synthetic food dyes in pharmaceuticals. The analytes are detectable at a concentration level 0.3-0.8 microg ml(-1).

Azo Compounds↗
PubMed2006

[Sentinel lymph nodes identification in vulvar cancer--methods and technique].

OBJECTIVE: To evaluate detection of sentinel lymph nodes (SLN) in squamous vulvar cancer with blue dye and 99mTc. The study describes technique of application, timing, management, detection rate (DR), specific side detection rate (SSDR) and false negative rate. DESIGN: Prospective clinical study. SETTING: Department of Obstetrics and Gynecology, Charles University Prague, 2nd Medical Faculty, Teaching Hospital Motol. PATIENTS AND METHODS: 46 women with squamous cell carcinoma tumors stage I or II, <4 cm with no clinical suspect lymph nodes were included. Blue dye alone was used in 16 women and the combination of 99mTc and blue dye was used in 30 women. Radiocoloid 99mTc was applied 3-5 hours and blue dye 3-5 minutes prior to ingvinal incision. RESULTS: We detected 88 SLN in 61 inguinal spaces. The detection rate in the blue dye group was 68.8 % (11 cases). One false negative SLN (6.3 %) appeared in this group. In blue dye+ 99mTc group detection rate was 100 % with no false negative SLN. CONCLUSION: Detection of SLN in squamous vulvar cancer with the combination of 99mTc and blue dye was statistically significantly more effective than using the blue dye alone.

Adult↗
PubMedDate not supplied

[Use of dye binding for quantitative determination of proteins in solutions (review)].

A review is presented of the methods used for estimation of protein content in solutions. These methods are based on measuring of some organic dyes binding with protein--amido-black 10B, coomassy brilliant blue R-250 and G-250, ponso G, violet sulfate, bromphenol blue, naphthol yellow S, orange G. Advantages and limitations of these methods were compared with Lowry et al (1951) method and with some other conventional methods for estimation of protein content. The methods based on dye binding might provide additional information and substitute the Lowry method if an adequate protein is selected for a calibration plot.

Amido Black↗
PubMed1985

Structure and biological activity of polysomes stained with Coomassie blue.

Polysomes prestained with Coomassie blue were fractionated on sucrose density gradients giving rise to visible bands corresponding to different size classes of aggregates. Coomassie blue staining enhanced the capacities of brain and liver polysomes to synthesize proteins in vitro, including the synthesis of neuron-specific enolase. This positive action of the dye was restricted to polysomes and was not manifested when mRNAs isolated from prestained polysomes were tested in in vitro translation or reverse transcription, indicating that the action of the dye consists in stabilization of polysomal structure.

Animals↗
PubMed2006

Biodegradation of dye solution containing Malachite Green: optimization of effective parameters using Taguchi method.

In this paper, optimization of biological decolorization of synthetic dye solution containing Malachite Green was investigated. The effect of temperature, initial pH of the solution, type of algae, dye concentration and time of the reaction was studied and optimized using Taguchi method. Sixteen experiments were required to study the effect of parameters on biodegradation of the dye. Each of experiments was repeated three times to calculate signal/noise (S/N). Our results showed that initial pH of the solution was the most effective parameter in comparison with others and the basic pH was favorable. In this study, we also optimized the experimental parameters and chose the best condition by determination effective factors. Based on the S/N ratio, the optimized conditions for dye removal were temperature 25 degrees C, initial pH 10, dye concentration 5 ppm, algae type Chlorella and time 2.5h. The stability and efficiency of Chlorella sp. in long-term repetitive operations were also examined.

Animals↗
PubMed2003

Methylene blue dye as an alternative to isosulfan blue dye for sentinel lymph node localization.

BACKGROUND: Our study describes the use of methylene blue dye as an alternative to isosulfan blue dye to identify the sentinel lymph node (SLN). METHODS: A retrospective analysis was performed of 112 breast cancer patients (113 axillae) who underwent SLN biopsy (SLNB) with methylene blue dye and (99m)Tc-labeled sulfur colloid for SLN identification. All SLNs were submitted for intraoperative frozen section analysis, hematoxylin and eosin stain, and immunohistochemical evaluation. Patients with a pathologically negative SLN did not undergo further axillary lymph node dissection. RESULTS: Of 112 patients who underwent SLNB, the SLN was identified in 107 (95.5%); 104 (92.8%) were identified by methylene blue dye. In a subset of 99 patients with recorded isotope status in relation to blue nodes, concordant identification with both dye and isotope was observed in 94 (94.9%). Of patients with identified SLNs, 32 (29.9%) of 107 contained metastatic disease, with 31 (96.9%) of 32 identified by methylene blue dye. The SLN was the only positive node in 18 (60.0%) of 30 patients. CONCLUSIONS: SLNB with methylene blue dye is an effective alternative to isosulfan blue dye for accurately identifying SLNs in breast cancer patients.

Adult↗
PubMed2002

Background-free, fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes, zincon and ethyl violet.

A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion-pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet-colored bands. It is a rapid and end-point staining procedure, involving only fixing and staining steps that are completed in 1-1.5 h. The detection limit of this method is 8-15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye-based stains for routine laboratory purposes.

Azo Compounds↗
PubMedDate not supplied

An investigation of new commercial samples of methyl green and pyronin Y.

New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magnetic resonance, in addition to standardized simultaneous and sequential staining methods using purified Ethyl Green and pure Pyronin Y as reference dyes. The Methyl Green was found to be Ethyl Green contaminated with Crystal Violet. It did not have any advantages compared with Ethyl Green supplied by American dye companies. The Pyronine G sample was Pyronin Y with a high dye content that gave good staining results when used with purified Ethyl Green. Pyronin Y from Polysciences was found to be essentially pure Pyronin Y.

Chromatography, Thin Layer↗
PubMed1992

In vitro evaluation of dynamic fluid displacement in dentinal tubules activated on pin placement.

The use of cemented, friction-lock, and self-threading pins for improving retention has been essential for treatment in restorative dentistry, with the self-threading pin considered to be the most retentive. Cavity varnish has been suggested to prevent microleakage around pins. This study investigated the insertion of a self-threading pin when the pinhole was filled with a liquid dye. The results showed that pinholes filled with dye before pin placement had measurable dye displacement. Pinholes without pins displayed no measurable dye displacement through the dentin; this was also true when the dye fluid was removed before the pin placement. However, the dye in pinholes was displaced toward the path of least resistance, and fluid in a pinhole can contribute to crazing of the dentin during placement.

Dental Leakage↗