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PubMed1992

Description and evaluation of a simple method for microbiological studies on otitis externa.

A simple cultural method for identification of the bacteria and yeasts likely to cause external otitis in divers is described. The method employs a commercial dip-slide system (Urotube) in which ear swabs are rolled onto the three agar surfaces, spread by a loop, and then incubated for a period of 18 h or more at 37 degrees C. The method was compared with traditional culture methodology on agar plates. Use of reference strains of bacteria and stored clinical isolates of bacteria and yeasts from ear infections showed stable patterns of growth for each species. Semiquantitative investigation of growth showed that there was a constant relationship between amount of growth on agar plates as compared to the dip-slide. Results were reproducible and were not affected by prolonged incubation. Directly inoculated swabs from normal ear canals and clinically infected ear canals showed identical findings by the traditional culture and Urotube methods. The Urotube method has also been evaluated in practical situations offshore with favorable experience. The Urotube method thus provides a rapid and simple way of identifying the causes of external otitis in divers in isolated areas where laboratory facilities are not readily available. This can have consequences for speed and choice of therapy in isolated environments.

Culture Media↗
PubMed1991

[Vibrio alginolyticus and swimmer's otitis externa. 2 cases and review of the literature].

We describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the Mediterranean shore, with Vibrio alginolyticus recovered from ear fluid. We describe the biochemical profile and sensitivity pattern (MIC) of both strains, comparing it to previously published data. A literature review was also performed, in which we found evidence for increasing concern of V. alginolyticus as an human pathogen. Also there is a need for considering halophilic vibrio as potential pathogens, specially if there is also epidemiologic support.

Adult↗
PubMed1994

Invasive otitis externa due to Aspergillus species: case report and review.

Aspergillus flavus, an unusual cause of malignant external otitis, was identified in pure culture of tissue from two patients in which histologic examinations demonstrated branching septate hyphae invading the temporal bone. Both patients presented with protracted external ear pain. Multiple attempts at treatment with topical agents (polymyxin B sulfate-neomycin sulfate-hydrocortisone), oral agents (cephalexin and ciprofloxacin), and parenteral agents (ceftazidime and tobramycin) were unsuccessful before definitive diagnosis was made. Treatment with amphotericin B, followed by a more protracted course of itraconazole, resulted in an apparent cure for these patients. Follow-up examination at 15 months after the discontinuation of therapy with itraconazole demonstrated no recurrence of infection. Previously reported cases have predominantly involved immunocompromised persons, and these cases are summarized.

Aged↗