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At least 415 records · Page 23Linked to original sources
PubMed1986

[Use of the antibody neutralization test for detecting myoglobin in the urine in the diagnosis of myocardial infarction].

A total of 230 patients admitted to Moscow city hospitals were examined to study the diagnostic value of a kit "Diagnosticum for the detection of erythrocytic myoglobin, dry". Myocardial infarction was diagnosed in 165 of them: in 138 it was transmural, in 27 intramural and in 65 the diagnosis was not confirmed (they were entered into the control group). Myoglobinuria was detected on the 1st day of transmural MI in 95%, on the 2nd day in 92% and on the 3rd day in 82%. In patients with intramural MI myoglobinuria was noted in 72% of the cases. In the control group the test was false positive in 9%.

Adult↗
PubMed1988

Human immune response to botulinum pentavalent (ABCDE) toxoid determined by a neutralization test and by an enzyme-linked immunosorbent assay.

To determine the immune status of persons receiving botulinum pentavalent (ABCDE) toxoid and to evaluate the effectiveness of the vaccine, we surveyed immunized individuals for neutralizing antibodies to type A and to type B botulinum toxins. After the primary series of three immunizations administered at 0, 2, and 12 weeks, 21 of 23 persons tested (91%) had a titer for type A that was greater than or equal to 0.08 international units (IU)/ml, and 18 (78%) had a titer for type B of greater than or equal to 0.02 IU/ml. (One international unit is defined as the amount of antibody neutralizing 10,000 mouse 50% lethal doses of type A or B botulinum toxin). Just before the first annual booster, 10 of 21 (48%) and 14 of 21 (67%) people lacked a detectable titer for type A and for type B, respectively. After the first booster, all individuals tested had a demonstrable titer to both types A and B. Of 77 persons who had previously received from one to eight boosts of the toxoid, 74 (96%) had an A titer of greater than or equal to 0.25 IU/ml and would not require an additional booster, according to the recommendations of the Centers for disease Control. However, only 44 of 77 (57%) had a B titer of greater than or equal to 0.25 IU/ml. In each group by booster number, even the group having had eight boosts, at least one person would require reimmunization on the basis of B titer. There was a wide range of antibody levels among individuals at the same point in the immunization scheme. Results from an enzyme linked immunosorbent assay, with purified type A or type B neurotoxin as the capture antigen, were compared with neutralization test results on 186 serum samples for type A and 168 samples for type B. Statistically, the correlation coefficients for results from the two assays were high (r = 0.69, P < 0.0001, for type A and r = 0.77, P < 0.0001, for type B). However, due to the wide dispersion of values obtained, using enzyme-linked immunosorbent assay results to predict neutralizing antibody levels is unwarranted.

Antibody Formation↗
PubMed1974

The diagnosis of rabies in a horse by brain neutralization test.

A horse showing clinical signs of a neurological disorder was killed and various diagnostic tests for rabies were carried out. Histopathlogy revealed a nonsuppurative encephalitis. Fluorescent antibody test and mouse inoculation test were negative. A positive diagnosis of rabies was based on a high antibody titer (1:10,000) to rabies virus in brain tissue.

Animals↗
PubMed1993

[Determination of effectiveness of FMD vaccine type Asia 1 in neutralization tests in cattle].

On June 20th 1984 FMD type ASIA 1 appeared in two herds of cattle for the first time in Europe (Greece). All affected and suspected cattle (1200) were killed on July 4th to 13th 1984. Blood samples of 125 of these animals were collected and examined by neutralisation test. All susceptible animals in this area as well as of buffer zone were vaccinated against ASIA 1 (from FAO, produced by IFFA Merieux). In three weeks after this first vaccination blood samples from 148 animals were collected, examined and the results of the neutralisation test were correlated with the effectiveness of the vaccine. 17.2% from these animals have been found to have neutralisation titers less than 1.2.

Animals↗
PubMed1976

Analysis and optimization of a quantitative organic soil neutralization test for disinfectants.

An analysis and optimization of a test to quantitatively assess the resistance of disinfectants to neutralization by organic soil is presented. The recommended method uses sterile, dry baker's yeast as a standard organic soil and 24-hr cultures of Staphylococcus aureus and Pseudomonas aeruginosa as test organisms. The test determines the maximum per cent (w/v) of organic soil which a disinfectant can tolerate and remain able to kill about 106 test organisms/ml in 10 min at 25 degrees C. This per cent is defined as the organic soil neutralization number. The procedure offers several advantages over other organic soil capacity tests.

Bacteriological Techniques↗
PubMed1973

Assay of variola virus on microtiter plates and its application to the neutralization test.

It was established that microfocus assay of the variola virus in HeLa cell cultures on Microtiter plates is a suitable method for titrating the virus in numerous specimens of small volume. The variola foci were formed within 48 hours of inoculation, and the standard error for the number of foci per well did not exceed 15%, with 96 flat-bottomed wells (6 mm diameter) containing 0.1 ml per well of the culture media on a plastic plate replacing 96 Petri dishes in the standard assay procedures. Microfocus assay was successfully applied to the quantification of neutralizing antibody against variola and vaccinia viruses, with a reproducibility equal to that of the standard focus reduction method on glass Petri dishes. Applications of this technique to the assay of field specimens and to the laboratory differentiation of variola viruses are discussed.

Antibodies, Viral↗
PubMedDate not supplied

[Comparative value of temperature marker and neutralization test for subtyping of herpes simplex viruses (author's transl)].

Two methods for the differentiation of herpes simplex virus (HSV)--rct and neutralization by specific antisera--have been applied to 8 prototype strains and 105 strains isolated from various clinical cases. The evaluation of the II/I index allows to choose the most specific rabbit antisera. The rct 40 degrees 3 C marker test gives comparable results for HSV grown in RK13 and human fibroblasts as well; it is in agreement with the immunological test in 48% of the cases. The delta tc values used to express the immunological typing allow to differentiate 76% of the strains (types 1 and 2). The others (24%) are equally neutralized by both specific antisera; they represent "intermediate" strains. The infection of man and animal by HSV (type 1, 2 or "intermediate") also produces heterospecific antibodies in variable amount. From clinical and immunological data rises the hypothesis that "intermediate" strains derive from HSV1 strains by antigenic variation during a persistent infection.

Animals↗
PubMed2002

[Standardization of neutralization tests using the COBL cell line and comparison with the particle agglutination test for measles serology].

The aim of the present study was the detection and comparison of measles antibody titers with particle agglutination (PA) and neutralization (Nt) methods, in the sera samples of 364 subjects from different age groups. PA method was performed with a commercial test kit (Serodiameasles, Fujirebio Com. Japan), and Nt test which was standardized in this study, by using COBL (cord blood) cell lines, has been started to use in our laboratory as a reference method. As a result, antibody titers detected by PA were in parallel to the titers which detected by Nt test, and it was concluded that the differences in antibody titers would arise from the differences of test principles and viral antigens.

Adolescent↗
PubMed1974

Microadaption of hemadsorption inhibition for neutralization tests with pig hemagglutinating encephalomyelitis virus.

A microneutralization test is described, in which secondary pig thyroid cells are used and end points are determined by hemadsorption with hamster erythrocytes. The method is evaluated and compared with a macromethod, in which the fluorescent-antibody staining technique is applied for reading titers. The micromethod was found to be useful because of its sensitivity and rapidity.

Analysis of Variance↗
PubMed1969

The chick embryo neutralization test in the assay of meningococcal antibody. 2. Response of the embryo to meningococcal endotoxin and to infection.

A previous report described the infection of the chick embryo with Neisseria meningitidis. Although death of embryos was preceded by multiplication of the organisms, the possibility exists that death is caused by the endotoxin contained in the inoculum or released by the bacteria lysing in vivo. A second important condition for the establishment of a satisfactory sero-protection test is that the embryo should act as a host and not merely as a growth medium for N. meningitidis.Results of tests with cortisone-treated embryos show that the pathogenic effect of live meningococcal challenge is mainly due to infection elicited by the meningococci and not to toxaemia. Also, the rate of clearance of meningococci from the blood of the embryo, increased by the addition of meningococcal immune serum, suggests that at this stage of embryonic development (12 days) the phagocytes are active in combating meningococcal infection.

Animals↗