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PubMed1987

Development of postglucoprivic insulin-induced suckling and feeding in rats.

Increased food or milk intake in response to insulin-induced hypoglycemia cannot be demonstrated in the rat until pups reach weaning age. However, when food and suckling are withheld from insulin-treated 5- to 25-day-old rats until their altered blood glucose levels return to normal, their rate of milk intake via suckling from their anesthetized dam is increased over saline-treated control pups. This postglucoprivic action of insulin could not be demonstrated in rats consuming wet mash until pups reached 25-30 days of age. Nonnutritive oral stimulation from dry suckling during the glucoprivic episode is sufficient to disrupt postglucoprivic suckling in 20-day-old rats. In contrast consuming a small quantity of wet mash became an effective inhibitor of postglucoprivic suckling only when pups reached 25 days of age. These data demonstrate the existence of an insulin-sensitive neural system for suckling and feeding in infant rats and point to the involvement of multiple and changing oral factors during development in insulin-induced postglucoprivic feeding.

Animals↗
PubMed1975

Energy cost of square dancing.

This experiment was concerned with determining the energy cost of two popular Western square dancing routines: the "Mish-Mash," which is a relatively fast-moving dance with quick movements, and the "Singing" dance, which is a slower and more deliberate type of dance. The subjects were four middle-aged couples, veteran members of a local square dancing club. Sitting and standing pulmonary ventilations were determined through the use of the Tissot gasometer. Kofrańyi-Michaelis respirometers were employed for the dance routine ventilations. These apparatus were fitted with a Monoghan neoprene cushion plastic mask. Gas samples were collected in polyethylene metallized bags and analyzed for O2 and CO2 content. The net energy cost for the two dances was appropriately summarized. The results indicated that for the males the net average energy cost of the "Mish-Mash" dance was 0.085 and 0.077 kcal/min per kg for the "Singing" dance. For the females, the cost was 0.088 and 0.084 kcal/min per kg, respectively. A net average cost of these two dances yielded a caloric expenditure of 5.7 kcal/min for a 70-kg male and 5.2 kcal/min for a 60-kg female. It was indicated that during the course of a typical square dance evening, a 70-kg man would expend some 425 kcal. while a 60-kg female would burn some 390 kcal. The energy cost of the dances studied were determined to be within the permissible work load of a functional class 1 patient with diseases of the heart as determined by the American Heart Association.

Adult↗
PubMed1994

A chicken achaete-scute homolog (CASH-1) is expressed in a temporally and spatially discrete manner in the developing nervous system.

We have identified a basic helix-loop-helix encoding cDNA from embryonic chicken retina which shares sequence similarity with the achaete-scute family of genes of Drosophila. The deduced amino acid sequence of this chicken achaete-scute homolog (CASH-1) is identical, over the region encoding the basic helix-loop-helix domain, to the recently identified mammalian achaete-scute homolog (MASH-1) and to the Xenopus homolog (XASH1), and 70% identical, over the same region, to Drosophila achaete-scute complex members. The expression of CASH-1 is restricted to subsets of neuronal progenitor cells in the developing chicken nervous system, similar in distribution to that reported for MASH-1 and XASH1. In addition, in situ localization in the retina reveals a dynamic character of expression of the gene in a particular region of the CNS, and suggests that the expression of CASH-1 may be important in defining a particular stage in the progenitor cell necessary for the differentiation of particular neuronal phenotypes.

Amino Acid Sequence↗
PubMed1990

Effects of meals on gastric emptying and small intestinal transit times of a suspension in the beagle dog assessed using acetaminophen and salicylazosulfapyridine as markers.

Effects of the amount and the composition of meals on gastric emptying and small intestinal transit times of a suspension were investigated in beagle dogs using acetaminophen and salicylazosulfapyridine as markers. Gastric emptying time was affected both by the amount and the composition of a meal; it was prolonged proportionally to the amount of a solid meal and varied among the 3 kinds of test meals of the same energy content in the following rank order: lard greater than skimmed milk greater than mashed potatoes. The inter-individual variation of small intestinal transit time in a fed state was smaller than that in a fasted state, whereas the mean transit times in both states were similar. Small intestinal transit time was not affected by the amount of the solid meal. On the other hand, it varied among the 3 kinds of test meals in the following rank order: lard greater than mashed potatoes greater than skimmed milk. It is noteworthy that small intestinal transit time in the beagle dog is approximately 2 h shorter than that in humans both in fasted and fed states.

Acetaminophen↗
PubMed1980

Bacterial contamination in feed ingredients, formulated chicken feed and reduction of viable bacteria by pelleting.

The number of bacteria in steam-pelleted or crumbled chicken food was compared with the number in individual ingredients and in mash. A few bacteria were detected in white-fish meal processed Alaska pollack. Highest yields were from alfalfa meal. Colonies numbering 3.5 x 10(3)-4.2 x 10(5) were obtained by heart-infusion agar culture, and 2.1 x 10(2)-7.0 x 10(4) with deoxycholate hydrogen sulphide lactose agar, from 1 g mash. After pelleting or crumbling the number of colonies in 1 g was reduced to 0-1.9 x 10(3) and 0-3.0 x 10(0) respectively. Bacteria in the lactose medium, mostly coliform, were observed in only 1 of 18 samples.

Animal Feed↗
PubMed2005

Amplified fragment length polymorphism of the AWA1 gene of sake yeasts for identification of sake yeast strains.

Sake yeasts are used for sake brewing and have a crucial role in the quality of sake, since they produce not only ethanol but also various compounds that provide sake flavors. Therefore, the appropriate selection and monitoring of a strain used in sake mash is important. However, the identification of specific sake yeast strains has been difficult, because sake yeasts have similar characteristics in taxonomic and physiological analyses. We found amplified fragment length polymorphisms (AFLPs) in the PCR products of the AWA1 gene of sake yeast strains. The AWA1 gene encodes a cell wall protein that is responsible for foam formation in sake mash. This polymorphism of the AWA1 gene can be used for the identification of sake yeast strains.

DNA Fingerprinting↗
PubMed2002

Production of acetone butanol ethanol from degermed corn using Clostridium beijerinckii BA101.

In this article we report on acetone butanol ethanol (ABE) fermentation characteristics of degermed corn when using Clostridium beijerinckii BA101. Recent economic studies suggested that recovery of germ from corn and hence corn oil would help to make the ABE fermentation process more economical. C. beijerinckii BA101 ferments corn mash efficiently to produce ABE under appropriate nutritional and environmental conditions. Corn mash contains germ/corn oil that is, possibly, ancillary to the production of butanol during the ABE fermentation process. Since the presence of corn oil is not a critical factor in solvent fermentation, it can be removed and this will allow for byproduct credit. Batch fermentation of degermed corn resulted in 8.93 g/L of total ABE production as compared with 24.80 g/L of total ABE when supplemented with P2 medium nutrients. During the course of the germ separation process, corn steeping is required prior to grinding and removing the germ. It is likely that some nutrients from the corn are leached out during the steeping process. This may reduce the rate of fermentation and impact the final concentration of butanol/ABE that can be achieved. Fermentation of degermed corn with corn steep liquor resulted in the production of 19.28 g/L of ABE.

Acetone↗
PubMedDate not supplied

Effect of ochratoxin A on Escherichia coli-challenged broiler chicks.

A study was conducted to evaluate the effects of ochratoxin A (OA) on Escherichia coli-challenged broiler chickens. Day-old broiler chicks were separated into two groups of 92 chicks each, with one group fed a control mash diet, and the other fed a mash diet containing 2 ppm OA. On day 14, each group was further separated into two groups, with one group inoculated with E. coli O78 (1 x 10(7) colony-forming units/0.5 ml), whereas the other group was not inoculated with E. coli. After E. coli inoculation on day 14, four birds from each group were euthanatized at 1, 2, 3, 5, 7, 10, 14, and 21 days postinoculation. Escherichia coli infection caused dullness, depression, huddling, and diarrhea. Mortality was 14.3% in chicks infected with E. coli but fed no OA. Mortality increased to 35.7% in chicks fed OA and infected with E. coli. Decreased body weight and reduced feed intake were observed in chicks fed OA, and the effects were more pronounced in chicks fed OA and infected with E. coli. Increased serum levels of aspartate aminotransferase, alanine aminotransferase, uric acid, and creatinine and decreased levels of total proteins, albumin, globulins, calcium, and phosphorus were observed in OA-fed birds. Escherichia coli infection did not cause significant alteration in any of the serum biochemical parameters. The presence of OA in poultry rations increased mortality and the severity of an E. coli infection.

Animal Feed↗
PubMedDate not supplied

Diet-induced changes in the male reproductive system of rats.

Either quantitative or qualitative modification of diet interferes markedly with the reproductive functions of the rat. The effect of chick mash or cowpeas (Vigna unquiculata) on body growth, accessory sex organ weights, serum testosterone, and epididymal histology was studied. Body growth was markedly decreased in cowpeas fed group. Binucleated, multinucleated giant cells (MGCs), and lymphocytes were found in large numbers in the lumen of caput and cauda epididymis of the cowpea-fed group. No noticeable effect was seen in chick mash-fed group. Serum testosterone levels were significantly decreased in cowpea-fed group. Presence of MGCs in cowpea-fed animals indicates epididymal dysfunction and probably renders them infertile.

Animals↗
PubMed1982

Self-selection of calcium during the rearing and early laying periods of White Leghorn pullets.

The ability of pullets to selectively consume calcium to meet the requirements of the rearing and early laying period was studied in White Leghorns. In the first of two experiments, 456 pullets were fed either an .89% calcium rearing diet (control) or a .35% calcium diet in combination with a separate source of 38% calcium chick-sized grit (calcium self-selection) from 35 to 126 days of age. Twenty-four pullets from each of these rearing treatments were selected for the second experiment at 112 days of age and were housed in individual laying cages. Rearing treatments were continued until 176 days of age with mash and grit consumption recorded daily for each pullet. From 177 to 225 days of age these pullets were fed a 3.50% calcium diet containing either ground limestone or oyster shell as a calcium source or a .35% calcium diet fed in combination with a separate source of oyster shell. Pullet calcium intake reflected the requirements for growth during the rearing period, calcium storage in the medullary bone 19 days prior to lay, and egg shell formation during the laying period; average calcium intake for these periods was 1.18, 2.08, and greater than 3.50%, respectively. Pullets also demonstrated the ability to compensate for rearing deficiencies by increased calcium consumption when fed oyster shell on an ad lib basis. Calcium consumption was found to be significantly higher on days when an oviposition occurred than when no eggs were laid. Mash consumption during the early laying period also reflected the reproductive status of the hen with the highest consumption on days when both oviposition and ovulation occurred, intermediate consumption when either oviposition or ovulation occurred, and lowest consumption when neither oviposition nor ovulation was detected.

Aging↗
PubMed1984

The roles of protein level and diet form in water consumption and abdominal fat pad deposition of broilers.

Two experiments were conducted to investigate the possible relationship of water intake and water/feed ratios in explaining abdominal fat changes associated with changes in dietary protein level and diet form. In Experiment 1, day-old male chicks from two commercial broiler stocks were fed diets containing 26, 22, and 17% protein from 0 to 19 days of age. Chicks fed the 26% protein diet consumed more water, had higher water/feed ratios, and had less abdominal fat than chicks fed lower protein diets. Chicks fed the 22% protein diet were intermediate to the 26 and 17% protein treatments in water consumption, water/feed ratios, and abdominal fat. In Experiment 2, day-old male chicks from two commercial broiler stocks were fed a diet in either mash or crumbled form from 0 to 20 days of age. Chicks fed crumbles had heavier body weights, consumed more feed and water, and had more abdominal fat than birds fed the mash diet. Superior body weights of birds fed the crumbled diet appeared to be due to higher relative growth rates immediately after hatch.

Abdomen↗
PubMed1984

A comparison of the effect of two force molting methods on performance of two commercial strains of laying hens.

A low-sodium diet (.08% Na) was used to force molt 409 hens. Another 421 hens were force molted by conventional water and feed restrictions. Birds were 68 weeks of age at that time, and two strains were about equally represented in each treatment group. These strains were: A, DeKalb; and B, Hisex. The low-sodium diet and water were provided ad libitum to the first group for the entire period of molt (42 days). The second group received no water for 3 days, and no feed for 4 days, after which incremental amounts of whole oats were provided as the only feed until Day 18. After Day 18, incremental amounts of a regular laying mash and decremental amounts of whole oats were provided through Day 26, when laying mash only was given ad libitum. Both treatment groups were in the same building and received the same lighting program during the force-molt period. This program consisted of: Days 1 to 3, no light; Days 4 to 18, 8 hr/day; Days 19 to 25, 9 hr/day; Day 26, 10 hr; then, light was increased 30 min weekly until 14 hr daily was attained. The low-sodium group ceased laying after 28 to 31 days, lost 8.7% of their premolt body weight, reduced feed consumption by 35%, and decreased egg production from 62.3% (in the 28 days preceding the molt) to 19% in the 42 days of molt; their mortality rate was 3.4%.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗
PubMed1987

Effect of lighting program and nutrition on reproductive performance of molted single comb White Leghorn hens.

Two adjoining rooms in a light-tight, fan-ventilated, insulated house were used for a study involving 320 Single Comb White Leghorn hens, 60 wk of age, placed two per cage. These hens were subjected to an induced molt which compared two lighting programs, two molt rations, two levels of total sulfur amino acids (TSAA), and two levels of ascorbic acid (AA) in a factorial arrangement. There were four treatments. Treatment 1 compared the Washington lighting program (WSU), consisting of an 8-h light photoperiod for 28 days beginning 7 days before fast with the North Carolina program (NCSU), consisting of a 24-h light photoperiod for 7 days prior to fast followed by 12 h light/day for 21 days. After 28 days, light duration was increased to 16 h/day in stages for both programs. Treatment 2 consisted of feeding cracked corn (CC) or 16% protein molt ration (MR) for 2 weeks: Treatment 3, feeding of 14% layer mash with either .60% or .65% TSAA; and Treatment 4, addition of either 0 or 50 ppm AA to the 14% layer mash. After molting, egg production was increased in the NCSU lighting program and .65% TSAA treatments. Feed conversion was improved by the NCSU lighting treatment. Deaths were fewer in diets with 50 ppm AA. Egg weight, specific gravity, and shell weight were not affected by any treatment. A significant light X molt diet interaction occurred due to better performance of MR birds compared with CC birds in the NCSU lighting program, whereas on the WSU lighting program, CC produced better performance. These data indicated that combining features of various molt programs may not produce optimum results.

Animal Husbandry↗
PubMed1987

Effect of lighting program and nutrition on feather replacement of molted single comb White Leghorn hens.

This experiment concerned the induced molt of 320 60-wk old Single Comb White Leghorn hens placed two per cage in two adjoining sections of a light-tight, fan-ventilated poultry building. Treatments compared were: 1) lighting program: Washington (WSU) vs. North Carolina (NCSU). The WSU program used 8 h light/day for 28 days beginning 7 days before fast. The NCSU program used continuous light for 7 days prior to fast, followed by 12 h light/day for 21 days. The light portion of the photoperiod was then increased in steps in both treatments to 16 h. 2) Molt diet: cracked corn (CC) vs. 16% protein molt ration (MR) for 14 days. 3) Laying diet. A 14% protein mash calculated to contain .60% or .65% total sulfur amino acids (TSAA) or ascorbic acid (AA) at 0 or 50 ppm added to the laying mash. Day 1 was designated as the first day of photoperiod modification. Primary and secondary feather loss and subsequent primary feather growth were all significantly increased by the WSU light program at 56, 84, and 112 days. Total and average growth of new primaries was significantly increased by MR at 56 days and total primary feather growth by .65% TSAA at 112 days. No effects due to AA were observed. Photoperiod had the greatest effect on molt per se, with MR and .65% TSAA increasing early and late growth, respectively.

Animal Nutritional Physiological Phenomena↗
PubMed1989

Water consumption and fecal moisture of laying hens fed wheat middlings and corn-soybean-alfalfa meal diets.

Hens, housed on the floor and fed a diet containing 89% wheat middlings (WM) either as mash or pellets, consumed more feed and water than hens fed corn-soybean meal-alfalfa meal (CSA) diets with either .15, .50, or .75%. NaCl. Moisture content of the hens' feces was not affected by mash or pellet processing of the WM diet. Feeding the .75% NaCl-CSA diet, however, significantly increased fecal moisture beyond that observed with the other treatments. Caged layers fed the WM diet voided more feces (2.6 fold) than did caged layers fed the .50% NaCl-CSA diet, suggesting that wet litter problems observed in the floor pens of birds fed WM were the result of a greater fecal volume voided rather than a higher moisture content per unit of feces.

Animal Feed↗
PubMed1990

Comparative effects of dietary tannins in ducks, chicks, and rats.

Studies were conducted to compare the effects of feeding high-tannin sorghum (HTS)- and low-tannin sorghum (LTS)-based diets suboptimal in protein to ducks, chicks, and rats. In the first series of experiments, Savanna (HTS) depressed both growth and feed efficiency of chicks and rats when compared with animals fed RS-610 (LTS). In contrast, ducks fed Savanna-based diets exhibited greater weight gains, but poorer feed efficiency values than birds fed RS-610. In a second series of experiments, Pioneer 8333 (LTS) and DeKalb BR-64 (HTS) sorghum-soybean meal diets were fed to chicks, ducks, and rats. As compared with animals fed 8333, BR-64 depressed both growth and feed efficiency of chicks and rats, but did not significantly affect either parameter in ducks. The lack of effect of HTS on duck growth may have been due to the formation of tannin-protein complexes upon exposure of the ground grain to water. This theory was based on the following observations: 1) ducks consumed water immediately after eating to facilitate swallowing the dry-mash diet; 2) a large amount of feed was deposited on the bottom of each water trough and subsequently consumed; and 3) ground HTS, which had been soaked for 4 h, had virtually no assayable tannin after drying. However, despite the marked reduction in assayable tannin content of treated HTS versus HTS that was dried only, the former material still depressed growth and feed conversion of rats. Thus, the lack of a growth-depressing effect of sorghum tannins in ducks fed dry-mash diets is both unique and unexplained.

Analysis of Variance↗
PubMed1992

The effect of meal feeding on small intestine weight.

Two trials were conducted using feed forms of pellets and mash to determine whether parts of the digestive tract (small intestine) were enlarged by meal feeding. Results obtained show that meal feeding of broilers significantly increases small intestine weight for both mash and pellet broiler groups when compared with continuously fed broilers. The body weight, feed conversion, and mortality did not differ between the continuous-fed versus meal-fed groups. Pelleting the diet during the growing period (3 to 6 wk of age) significantly improved both growth rate and feed conversion of broilers.

Animal Feed↗
PubMed2006

Decay of dinitroaniline herbicides and organophosphorus insecticides during brewing of lager beer.

This article examines the fate of four pesticides that can be present during the brewing of lager beer. For this purpose, malted barley was spiked at 2 mg/kg with pendimethalin and trifluralin (dinitroaniline herbicides) and fenitrothion and malathion (organophosphorus insecticides). Analyses of pesticide residues were carried out by a gas chromatograph with an electron capture detector, and their identity was confirmed by gas chromatography-mass spectrometry. Cleanup was necessary for the malt and spent grain samples. Beginning with mashing and ending with the final product 4 months later, various samples (spent grain, sweet wort, brewer wort, and beer) were taken to determine the concentration of the targeted residual pesticides during the various beer making phases. In all cases, the residual levels recorded in sweet wort sampled after the mashing phase were below the respective maximum residue limits established by Spanish legislation for barley. Significant proportions of pesticide residues (17 to 40%) were retained on the spent grain. Applying the standard first-order kinetics equation (r > 0.91), the half-lives obtained for the four compounds during the storage of the spent grain (3.5 months) varied from 138 days (fenitrothion) to 192 days (malathion and pendimethalin). Herbicide residues practically disappeared (<0.3%) after wort boiling, whereas the percentages of the remaining insecticides, fenitrothion and malathion, ranged from 3.5 to 4.3%, respectively, at this time. No residues of dinitroaniline compounds were detected in young beer, whereas there was a significant reduction in fenitrothion (58%) and malathion (71%) residues during fermentation. Lagering and filtering processes also reduced the content of the organophosphorus insecticides (33 to 37%). Finally, after the storage period (3 months), the content of fenitrothion was reduced by 75%, with malathion residues being below its detection limit.

Aniline Compounds↗