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PubMed1990

Application of luminol-dependent chemiluminescence to assay opsonizing antibodies to procyclic forms of Trypanosoma congolense in the sera of dogs experimentally infected with heterologous stocks.

Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T. congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as 1:400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.

Animals↗
PubMed1989

[Determination of the luminol-dependent chemiluminescence of granulocytes].

The authors have defined the optimal conditions for the determination of granulocyte luminol-dependent chemiluminescence (LDCL) in normal donors; the granulocytes have been isolated from small volumes of blood. Granulocytic LDCL has been studied in granulocyte exposure to zymosan, zymosan-activated serum, phorbol-myristate acetate, and FMLP (formyl-L-methionyl-leucyl-phenylalanine) synthetic tripeptide. Significant individual differences in the granulocyte activities in normal subjects have been revealed.

Granulocytes↗
PubMed1989

Changes of immunological parameters in immunocompromised patients under selective decontamination of the digestive tract. Second Part: Investigations on the influence of drugs on zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes.

The influence of drugs used for selective decontamination, given in therapeutically effective concentrations, was examined in healthy controls and immunocompromised patients with hematologic systemic diseases by measuring the zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes. Drug-induced repression of phagocytic activity could usually be found both in healthy controls and in patients with hematologic systemic diseases. The combination of trimethoprim and sulfamerazine had a pronounced repressive effect. Such indications should be taken as a basis for further investigations in order to avoid additional iatrogenic restriction of defence. If possible, drugs with effects leading to repression of phagocytosis should not be used for selective decontamination.

Anti-Infective Agents↗
PubMed1989

[Luminol-amplified chemiluminescence evaluation of the microbicidal property of alveolar macrophages in response to drugs].

The present study was designed to evaluate the validity of luminol-dependent chemiluminescence (CL) to evaluate the microbicidal properties of alveolar macrophages (AM) in response to drugs. It was observed that production of CL is associated with H2O2 as well as O2-, 1O2, .OH. Furthermore, our data revealed that CL is dependent not only upon the extracellular release of these oxygen radicals, but also upon intracellular metabolic events, which thus allows a complete analytical study in any given state. It should also be noted from our data that the effect of antibacterial agents on CL was greatly affected by the source of the target cells. The defect of amplified CL in AM obtained from young rabbits might be attributable to immaturity of AM.

Animals↗
PubMed1989

Luminol-enhanced chemiluminescence of whole blood. Statistical analysis, and comparison of the responses of different subjects.

We studied the effects of different variables on luminol-enhanced chemiluminescence (CL) of whole blood, induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP), opsonized zymosan particles (OZP), or phosphate buffered saline (PBS). Use of correction factor based on blood neutrophil count and hemoglobin concentration decreased interindividual variation and improved normality of the frequency distributions of CL responses. In FMLP- and PBS-induced CL, day-to-day variation was not significant, but it was significant in OZP-induced CL. Although storage of blood samples for 8 hours decreased CL, the decrease was not evident in a study of initial activation, i.e. CL measured at one minute after application of FMLP. Our results suggest that FMLP-induced initial activation is well suited for epidemiological studies because it is easy to determine and is affected neither by day-to-day variation nor by storage of the blood samples for a few hours.

Adult↗
PubMed1985

Comparison of superoxide generation and luminol-dependent chemiluminescence with eosinophils and neutrophils from normal individuals.

Although eosinophilia is found in many allergic and hypersensitivity diseases, the function of the eosinophil is not clearly established. To evaluate and characterize this function, anticoagulated blood from normal subjects was separated into purified populations of both eosinophils and neutrophils by a modified method for Percoll gradients. With this separation procedure, highly purified populations of eosinophils (95.0% +/- 2.1%) and neutrophils (97.2% +/- 0.4%) were obtained. Functional response of these two isolated granulocyte cell types was measured by luminol-dependent chemiluminescence (CL) and superoxide generation to opsonized zymosan and phorbol 12-myristate 13-acetate (PMA). Both the eosinophil and neutrophil peak CL response and superoxide generation to zymosan (1 mg), in the presence of autologous serum (10%), were identical. In contrast, when PMA (10(-4) to 10(0) micrograms/ml) was the stimulant, eosinophil CL was at least twofold greater than the neutrophil light emission (1,595,741 +/- 122,435 cpm/5 X 10(5) cells vs. 765,448 +/- 24,171 cpm/5 X 10(5) cells; n = 6). This same differential in responsiveness was seen in superoxide generation. Thus, under certain conditions the eosinophil's respiratory burst may be greater than that of the neutrophil, and this differential in metabolic activity may contribute directly to the eosinophil's inflammatory potential.

Cell Separation↗
PubMed1986

Bovine pneumonic pasteurellosis: evaluation of interactions between bovine pulmonary lavage cells and Pasteurella haemolytica (biotype A, serotype 1), using a luminol-dependent chemiluminescence assay.

In vitro interactions of bovine pulmonary lavage cells (PLC) and pathogenic isolates of Pasteurella haemolytica biotype A, serotype 1, were examined, using a luminol-dependent chemiluminescence (LDCL) assay. The PLC containing high concentrations of bovine alveolar macrophages were incubated with living and heat-killed P haemolytica at bacteria to PLC ratio of approximately 1:1. Kinetics of the mean LDCL response of bovine PLC to heat-killed P haemolytica cells were characterized by a gradual increase in the amount of light emitted over 150 minutes followed by a slight decrease at 180 minutes. In contrast, the LDCL responses of reaction mixtures containing living P haemolytica were characterized by the development of a maximal response at 60 minutes followed by a continued precipitous decrease in light emission to background values by 150 minutes. Differences were not noticed in the LDCL response of PLC suspensions from the same cow to 3 P haemolytica isolates. In each instance, reaction mixtures containing heat-killed bacteria had a similar LDCL profile that was characterized by continuous production of light over 180 minutes, whereas all reaction mixtures containing living bacteria underwent a precipitous decrease in light emission, which eventually resulted in a complete cessation of chemiluminescence. The PLC suspensions from different cattle did not respond to bacterial stimuli uniformly, with respect to the amplitude or detailed nature of the LDCL profile. The time that lapsed between the addition of living P haemolytica to PLC suspensions and the complete cessation of chemiluminescence varied for different cows.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1985

Initiation of luminol-based luminescence by injection of a single reagent, coupled with enhancement of light-output.

We describe the development of a single-reagent addition to initiate the light output in the oxidation of 6-(N-(4-aminobutyl-N-ethyl)isoluminol) hemisuccinamide (ABEI-H) as the final step in solid-phase luminescence immunoassays. Up until now, the disadvantage of luminol-based luminescence was the need to inject three solutions to optimise stability and performance in immunoassays. By combining these reagents, (pseudoperoxidase, sodium hydroxide and hydrogen peroxide), it has become possible to develop a system which is stable over several hours, and which, almost as a "spin-off" product, has a light-output between 2 and 5-fold that of the original system at the same time hardly altering the reagent blank. Such a system has the advantage that it can be easily adapted to automatic closed systems when these become available for luminescence immunoassays. With this implementation, single-injection light-initiation reactions are not restricted to acridinium-ester or fluorescein isothiocyanate luminescence.

Ferritins↗
PubMed1986

Enhancement of the luminol test by means of light amplification.

The type of device commonly referred to as a "starlight scope" will amplify available light by a factor of approximately 17 000. The use of this device will permit an image to be formed by exceedingly small amounts of blood when reacted with luminol reagent. Modification of the apparatus is necessary to permit focusing at short distances.

Blood Stains↗
PubMed1985

[Effect of bacterial lipopolysaccharide (S. minnesota R 595) on the luminol-dependent chemiluminescence of neutrophilic granulocytes in cord blood].

The effect of bacterial lipopolysaccharide (S. minnesota R 595) (LPS) on the luminol dependent chemiluminescence (LUM-CL) of resting and phagocytosing polymorphonuclear leukocytes (PMN) in cord blood or healthy adults has been investigated. The inhibitory effect of LPS on the LUM-CL of phagocytosing cord blood PMN has been much more pronounced than on the LUM-CL of adult PMN. The emission curves showed a delayed reaction in cord blood PMN and in contrast to this finding an accelerated reaction in adult PMN. LPS stimulated the photone emission of resting PMN of both groups in comparable intensity.

Adult↗
PubMed1982

Interaction between human neutrophils and group B streptococci (GBS) and group antigens, monitored by Luminol-dependent chemiluminescence.

Interaction between the five serotypes of GBS and human neutrophils was investigated using Luminol-dependent chemiluminescence. A considerable variation in response, and a variable dependence pre-opsonisation was demonstrated both for while bacteria and HCl-extracts. These preliminary results may have relevance to clinical infection with GBS and demonstrate hitherto unrecognised differences in the nature of "group-specific" antigen extracts from GBS serotypes.

Antigens, Bacterial↗
PubMed1995

[Effect of pefloxacin on luminol-dependent chemiluminescence and leukocyte adhesion].

The in vitro action of pefloxacin on the luminole-dependent chemiluminescence and adherence of polymorphonuclear leukocytes of guinea pigs was studied within a wide range of the concentrations from 0.1 to 100 micrograms/ml. Pefloxacin did not influence the peak values of the chemiluminescence while in concentrations of 0.1 to 10 micrograms/ml it retarded its attenuation. After incubation of the leukocytes in the presence of 100 micrograms/ml of pefloxacin and the subsequent washing out the drug there was observed a decrease in the chemiluminescence. In concentrations of 0.1 to 100 micrograms/ml pefloxacin had no significant effect on the leukocyte adherence.

Animals↗
PubMed1995

[Effect of ciprofloxacin on luminol-dependent chemiluminescence and adhesion of leukocytes].

The in vitro effect of ciprofloxacin on the luminol-dependent chemiluminescence and adherence of polymorphonuclear leukocytes of guinea pigs was studied with the use of a wide variety of the drug concentrations from 0.1 to 1000 micrograms/ml under two different conditions: during incubation of the cells in the presence of the drug different concentrations and after the cell washing to remove ciprofloxacin. In concentrations of 1 to 100 micrograms/ml ciprofloxacin lowered the chemiluminescence at the average by 20 per cent and in a concentration of 1000 micrograms/ml the drug completely inhibited it. In concentrations of 0.1 to 100 micrograms/ml ciprofloxacin lowered as well the leukocyte adherence.

Animals↗
PubMed1994

A new technique for enhancing luminol luminescent detection of free radicals and reactive oxygen species.

A novel technique is reported which makes use of (1) an improved method for solubilizing luminol at neutral pH along with (2) the addition of dimethyl sulfoxide to stabilize superoxide anion in the solutions in which luminescence is detected. These improvements resulted in (1) very low blank values of luminescence and (2) an approximately 6-fold increase in sensitivity of detection of peroxide and superoxide, as well as stabilizing the superoxide radical anion. The technique can also be used to evaluate the availability of antioxidants in biological homogenates and fluids.

Antioxidants↗
PubMedDate not supplied

[Micro-determination of the intensity of luminol-dependent chemiluminescence of plasma].

The authors attempted to modify the macro-method for assessment of the intensity of luminol-dependent chemiluminescence by using small volumes of capillary blood, diluted 100-fold. They have demonstrated the compatibility of two methods for blood collection, from the vein and from the finger, on the basis of the rapid test of oxygen intoxication severity and individual sensitivity of the body to hyperoxia in vitro. Use of microvolumes of the sample for analysis will facilitate the clinical application of the test for the selection of the optimal HBO schemes.

Blood↗
PubMed1993

Enhanced eosinophil luminol-dependent chemiluminescence and complement receptor expression by platelet-activating factor and interleukin-5.

The cytokine interleukin-5 (IL-5) and the lipid mediator platelet-activating factor (PAF) have both been shown to be involved in eosinophil differentiation and activation. We have measured and compared the effect of PAF and IL-5 on human eosinophils in terms of their luminol-dependent chemiluminescence (CL) response and their expression of complement receptors, CR1 and CR3. Both IL-5 and PAF enhanced the eosinophil CL response. The optimal concentrations were 40 U/ml for IL-5, and 10(-6) M for PAF. The priming effect of IL-5 was slow and reached a maximal response after 90 minutes incubation. In contrast, the effect of PAF peaked early and declined during incubation. In the complement receptor study, only PAF was able to enhance CR3 expression (p < 0.05) while the effect of IL-5 on eosinophil complement receptor expression was negligible. These results provide evidence that both inflammatory mediator (PAF) and cytokine (IL-5) can activate eosinophils but the effects of IL-5 and PAF on eosinophil CL response appear to be distinct. The activation of eosinophils by PAF and IL-5 may occur through different mechanisms.

Asthma↗