Possible molecular mechanism of loss of homologous and heterologous gap junctional intercellular communication in rat liver epithelial cell lines.
We have previously characterized a series of rat liver epithelial cell lines that exhibit levels of gap junctional intercellular communication (GJIC) which are inversely related to their levels of expression of transformed phenotypes. Cells of the non-tumorigenic line do not communicate with their tumorigenic counterparts. We have examined the molecular mechanisms involved in this loss of homologous and heterologous GJIC, employing a non-tumorigenic cell line, IAR 20, and a tumorigenic cell line, IAR 6-1. While both cell lines expressed a transcript coding for the gap junction protein, connexin 43 (cx 43), and similar levels of cx 43 protein, they exhibited different phosphorylation states of this protein, revealed by Western analysis. Immunohistochemical analysis showed that the non-tumorigenic IAR 20 cell line, but not the tumorigenic IAR 6-1 cells, was able to incorporate cx 43 gap junction plaques extensively into their plasma membranes. When IAR 20 and IAR 6-1 cells were co-cultured, cx 43 proteins were abundant in IAR 20 cells but IAR 20/IAR 20 cell boundaries were cx 43-positive, while IAR 20/IAR 6-1 boundaries were negative. The different phosphorylation state of cx 43 may partially explain the low GJIC of the IAR 6-1 cells and inability to communicate with their non-tumorigenic counterparts, but other mechanisms such as cell-cell recognition processes may also be involved.