Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “INSEMINATION”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 415 records · Page 23Linked to original sources
PubMed1977

Relationships between number of sperm inseminated, number of eggs laid and fertility in turkeys.

Three trials were conducted in which a series of different quantities of semen was used for inseminations. Relationships between total number of sperm inseminated (TNS) and fertility and number of viable sperm inseminated (NOV) and fertility were examined. Interrelationships between numbers of sperm inseminated, number of eggs laid, and fertility were also examined. It was found that TNS and NOV were significantly correlated (P less than .05) with fertility. When the number of eggs laid was considered (sperm to egg ratios), correlations were highly significant (P less than .01). Multiple correlation using number of sperm inseminated and number of eggs laid as the independent variables showed that TNS and the number of eggs laid were significantly correlated (P less than .05) with fertility. When the number of sperm inseminated was corrected so that only live, normal, and motile sperm were considered, the correlation became highly significant (P less than .01). It was concluded that the number of sperm required for optimum fertility was dependent on the number of eggs laid.

Animals↗
PubMed1988

Effect of extender viscosity on the insemination dose for chickens.

When semen was diluted 1:2 with Beltsville poultry semen extender (BPSE) containing 4% (w/v) carboxymethyl cellulose (CMC), seminal viscosity was 14 times greater than that of semen similarly extended with BPSE and 2.2 times more viscous than neat semen. Under these conditions, CMC was not spermicidal (P greater than .05). Increased seminal viscosity did not enhance fertility (P greater than .05) over a 20-day interval following a single insemination of 33 or 100 X 10(6) spermatozoa/hen. In the case of semen extended with BPSE as well as BPSE containing CMC, the insemination dose of 100 x 10(6) spermatozoa gave superior fertility (P less than .05) compared with results of insemination with 33 X 10(6) spermatozoa. However, when fertility data from the 1st wk of egg collection were compiled, there was no difference in fertility (P greater than .05) due to insemination dose. These results demonstrate that although a conventional insemination dose could be reduced in order to use semen more efficiently, increasing seminal viscosity may not be warranted for artificial insemination programs.

Animals↗
PubMed1997

Donor insemination: attitudes of parents towards disclosure.

OBJECTIVE: To determine attitudes of parents of children conceived by donor insemination to telling the children of their origin. DESIGN: Questionnaire survey, in 1992-1993, of parents who had had a child by donor insemination at four New South Wales clinics from 1979 to 1990. SETTING: Three hospital-based clinics (two public, one private) and a private clinic service. PARTICIPANTS: 276 families who altogether had had 420 children by donor insemination. MAIN OUTCOME MEASURES: Number of children told that they had been conceived by donor insemination; parents' intentions, before conceiving and after the birth, of informing the child; and whether other people were informed of the child's origin. RESULTS: Of 393 families who could be contacted, 353 agreed to participate and 276 (70%) replied to the questionnaire. Only 22 of the 420 children (5.2%) had been informed of their origin. Before conceiving, 18% of parents (49/273) planned to tell the child. Seventy-one per cent of families (182/257) had told others of the origin of the child, but 94% (241/257) had not told the child. Of the 29% of families (75/257) who had not told others of the child's origins, none had told the child. As the children grow older, more parents decide not to tell them of their origin. CONCLUSIONS: Most parents of children conceived by donor insemination do not plan to tell their child, but most of these parents have told others, creating the potential for accidental disclosure. Because of the small number of children who are told of their origin, there may not be a need for government-regulated donor registers, provided donor insemination units maintain a high standard of record-keeping.

Adult↗
PubMedDate not supplied

Infectious complications of intrauterine insemination: a case report and literature review.

Intrauterine insemination is commonly performed in the treatment of infertility. Infectious complications associated with intrauterine insemination are frequently cited, though rarely reported. A review of the literature yields only five reported cases of pelvic infections subsequent to intrauterine insemination (IUI), and only two of these show firm evidence of infection, with none presenting bacteriologic confirmation. We report a case of Escherichia coli septicemia subsequent to an IUI performed on a patient with a large adenomyoma. Of 147 patients treated with IUI at Georgetown University from November 1987 through March 1990, this was the only infectious complication. The incidence of infectious complications in our series is thus 0.0068 (6.8 per 1,000 women). A review of infectious complications in 38 reported series on intrauterine inseminations reveals five infections in 3,129 patients. With the addition of our series to the literature, the prevalence of infectious complications is 1.83 per 1,000 women undergoing IUI. The rates were not significantly altered by semen washing with antibiotics, or the administration of prophylactic antibiotics to the woman (P = .81). We conclude that (1) the infection rate following IUI is small, (2) many of the infections subsequent to insemination were not associated with intrauterine insemination, (3) most reported cases of infection fail to show evidence for the actual presence of infection, and (4) the prevalence is unaltered by the administration of prophylactic antibiotics or washing the semen sample with antibiotics.

Adult↗
PubMed1991

[Intrauterine and intracervical insemination with frozen donor semen. A comparative study].

Intracervical insemination (ICI) with cryopreserved donor semen was performed in 38 women. These inseminations were performed about the time of ovulation twice per cycle in a total of 215 cycles during the period 1.1.1989 to 31.12.1990. Nine of these women became pregnant corresponding to 4% (9/215) per insemination cycle. From 1.12.1989, intrauterine insemination was introduced (IUC) with swim-up/wash-prepared cryopreserved donor semen prepared in the department. Twenty-two women who had undergone from three to 12 (average 6.1) ICI treatment cyclic without resulting pregnancy were submitted to IUI with cryopreserved donor semen at the time of ovulation once per cycle for a total of 32 cycles during the period 1.12.1989 to 31.12.1990. Ten of these (45%) became pregnant corresponding to 31% per insemination cycle. The reason for this significant improvement in the results as compared with ICI is to be found not only in alterations in the semen preparations and the technique of insemination but also in consequent employment of hormone stimulation and meticulous monitoring of the cycles. Employment of the described therapeutic schema for "programmed cycles" is recommended in order to minimize the need for resource-demanding cycle monitoring and to avoid weekend work.

Adult↗
PubMed1990

Pregnancy rates after double versus single insemination with frozen donor semen.

Ninety-nine females (mean age 34 years +/- 1) were seen for therapeutic donor insemination using frozen semen. All patients used over-the-counter urinary ovulation predictor kits, with the insemination scheduled either the day after the positive test (group I; n = 46) or consecutively the day of and the day after the positive test (group II; n = 53). Group I patients underwent a total of 113 cycles of insemination (mean 2.5 cycles/patient). Seven pregnancies were achieved in group I for an overall success of 15.2% and a monthly fecundability of 0.06. Group II patients underwent a total of 100 cycles of therapeutic donor insemination (mean of 1.9 cycles/patient). Twenty-one pregnancies were achieved in this group for an overall success rate of 39.6% and fecundability of 0.21. The estimated cumulative probability of conception (F) for 6 months was 0.32 in group I and 0.78 in group II. These data indicate that the F after therapeutic donor insemination with frozen semen is greater if two consecutive inseminations are performed at midcycle.

Cryopreservation↗
PubMed1989

HIV serology in artificially inseminated lesbians.

The purpose of this investigation was to examine the risks of HIV transmission from artificial insemination in a sample of lesbians residing in California and inseminated between 1979 and 1987. This population was selected because lesbians are considered to be at low risk for HIV infection, and have traditionally used semen from men engaging in high-risk behaviors, namely, homosexual men. Each of the 98 participants had blood drawn for the HIV antibody test (ELISA) and completed a questionnaire inquiring about her sexual, health, and reproductive history, including detailed information about her inseminations (e.g., vaginal vs. uterine, fresh vs. frozen semen, sexual orientation, and antibody status of donor). One-half of the women had homosexual or bisexual donors, many of whom resided in the San Francisco Bay area; most of these inseminations were with fresh semen. The women reported a marked decline in the use of homosexual donors after 1982 and a corresponding increase in the use of donations from sperm banks and health practitioners. Based on the women's reports, as many as 11 women may have received semen from an infected donor. However, none of the 98 women tested seropositive. We attribute our negative findings to the change to low-risk donors in the years when HIV became more prevalent in the population, and to the potentially lower rates of infectivity with artificial insemination than with heterosexual intercourse. Nevertheless, we recommend that women continue to follow the CDC guidelines for screening donors prior to artificial insemination.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Economic modeling of the use of gonadotropin-releasing hormone at insemination to improve fertility in dairy cows.

Economic and sensitivity analysis methods were used to evaluate financial returns from use of gonadotropin-releasing hormone (GnRH) at the time of insemination to enhance fertility of dairy cows. A computer spread sheet was used to determine the best service(s) for GnRH treatment, the increase in conception rate required for economic benefit from treatment, and how profits from GnRH treatment are affected by drug cost, herd reproductive efficiency, and production costs. Financial returns increased from use of GnRH at insemination under most herd conditions. Herds with conception rates less than or equal to 45% benefited from GnRH treatment at any 1 or 2 inseminations. Herds with conception rates greater than or equal to 60% benefited from GnRH treatment only at second or later services. Selection of second and/or third insemination as the GnRH treatment service usually resulted in the greatest total return. The enhancement of fertility necessary to achieve the break-even point with GnRH treatment at third service was 2% for low- and 5% for high-conception-rate herds. Base-line herd conception rates, estrus detection efficiency, replacement costs, value of excess days not pregnant, and cost of treatment had the greatest effect on returns from treatment. Herds with high conception rates and low replacement costs were likely to realize the least benefit from GnRH treatment at insemination. On the basis of our findings, we concluded that GnRH treatment at insemination is a profitable procedure under most herd conditions. Optimal treatment regimens for specific herds may best be determined by using herd performance and management data for calculating returns.

Animals↗
PubMed1986

[The effect of Oestrophan Spofa (synthetic analog of prostaglandin F2 alpha) added to the insemination dose on pregnancy and fertility in sows].

Laboratory trials were conducted to study the effect of various concentrations of cloprostenol on the motility and morphological changes of the acrosomes of boar spermatozoa. As found, a cloprostenol concentration of 250 ng per ml of semen to 2500 ng per ml of diluted semen has no adverse effect on the motility of spermatozoa and on the morphological changes of their acrosomes. The concentration of 5000 ng of cloprostenol (in the Oestrophan Spofa product) per 1 ml of diluted semen negatively influences the motility of spermatozoa. An insemination dose of 100 ml of diluted sperm treated with 500 ng of cloprostenol was used for the artificial insemination of 152 sows; 166 sows of the same farm inseminated with untreated semen were used as controls. No gilts were included in the trial. Out of the 152 test sows, 113 conceived after the first insemination, i.e. 74.35%, and the average litter size was 10.04 piglets. In the control group, 125 sows delivered their litters, i.e. 75.30% of the total number, the average litter size being 9.96 piglets. Comparing the reproduction parameters of the experimental and control groups it can be said that the treatment of an insemination dose with 500 ng of cloprostenol immediately before insemination had no influence on the pregnancy rate and on the size of litters.

Animals↗
PubMed1974

[Synchronization of estrus by the use of TURISYNCHRON-premix and insemination time in young gilts].

In 664 primiparous gilts inseminated twice after oestrus synchronization, the timing of insemination had an important effect on conception rate and litter size. Best results (586 liver piglets per 100 pregnancies) were achieved by a first insemination on the afternoon of the 6th day. Another trial, with sexually mature gilts which were subsequently slaughtered, invloved artificial insemination at a planned time after synchronization of oestus with TRUISYNCHRON plus injection of Prolosan. Pregnancy rate and embryo count on the 29th day after synchronization were good when the first insemination was made on the evening of the 5th day and the second on the evening of the 6th day. Insemination on the morning and evening of the 6th day gave better results than on the evening of the 5th and morning of the 6th day.

Administration, Oral↗
PubMed1984

The cervical factor of infertility and intrauterine insemination.

Results with intrauterine insemination in 25 couples are presented. The indication for this procedure was a cervical factor of infertility. After an extensive fertility survey in both partners, intrauterine insemination was performed four to seven times per cycle with up to 0.8 ml fresh semen per insemination. Only the first ejaculatory spurt was used. Prophylactic antibiotics were given to all female partners during the insemination period. Within a mean of 6 months 13 couples reported pregnancies (52%). Except for one midtrimester abortion that was probably due to obstetrical reasons, the abortion rate was 33.3%. There were no side effects related to the insemination procedure. Our data indicate that, with appropriate safeguards, intrauterine insemination should be regarded as a safe and effective method for the treatment of the cervical factor of infertility.

Adult↗
PubMed1984

[Orienting study on the treatment of cattle with human chorionic gonadotropin on the day of insemination].

A report on two experimental studies in which 1,500 I.U. of HCG were administered to dairy cows on the day of insemination. The first experimental studies were done in 113 subfertile animals in which clinical examination did not reveal any clinically perceptible changes. HCG was injected intravenously in the animals of the experimental group, 0.9 per cent sodium chloride being injected intravenously in the controls. The pregnancy rates were 47 (sixty-one animals) and 30 per cent (fifty-two animals) in the experimental group and in the controls respectively. This difference was significant (P = 0.047). The ultimate pregnancy rates were 66 and 58 per cent (P = 0.32) respectively. The other experimental studies were done on a single dairy herd. The animals to be inseminated all were alternately assigned to an experimental group and a group of controls, regardless of the sequence of insemination. The pregnancy rates in the experimental group and the group of controls were 40 (ninety-eight inseminations) and 43 per cent (ninety-six inseminations) respectively. In concluding, it can be stated that routine administration of HCG to cattle with a view to increasing the pregnancy rate of the first or second insemination cannot be expected to be effective. Treatment with HCG may possibly be successful in a particular group of cattle which have difficulty in becoming pregnant.

Animals↗
PubMed1994

[Intrauterine insemination with donor semen. Treatment of involuntary childlessness caused by male infertility].

Intrauterine insemination with cryopreserved donor semen was performed in 114 women in a total of menstrual 381 cycles during the period 1.2.1991 to 15.5.1993. Eighty-one pregnancies were recorded corresponding to a conception rate of 21% per insemination cycle. After ten cycles the probability of conception and expected delivery was respectively 96% and 86%. The results show that intrauterine insemination with donor semen is a good treatment for couples with male infertility. Ultrasonic measurement of the follicles and timing of hCG injection were used for optimal timing of intrauterine donor insemination. The quality of the semen used for insemination is important. It is recommended to inseminate with at least two million of spermatozoa where at least 20% have normal motility.

Female↗
PubMed1993

Results of 527 controlled artificial inseminations in dogs.

During 1990 and 1991, 527 artificial insemination (AI) procedures were performed by 40 veterinarians, under the Swedish Kennel Club control scheme, and reported to the Kennel Club within 14 days. The most common reason for AI (37.2%) was import of fresh and frozen semen or quarantine, i.e. introduction of new genetic material. Fresh semen (468 AIs) was deposited into the cranial vagina, and frozen semen (59 AIs) was inseminated transcervically into the uterus. Pregnancy rates were 54.7% with fresh and 39.0% with frozen semen. When corrected for stage of oestrus at the time of AI and semen quality, the pregnancy rates were 62.3 and 51.1%, respectively. Bitches inseminated with frozen semen had a 29.7% lower pregnancy rate and 30.5% smaller litter size than bitches receiving fresh semen; these differences were statistically significant. Forty-four bitches (8.4%) were also mated. Their pregnancy rate was significantly higher (88.6%; 90.7% corrected) than that of bitches inseminated with fresh semen only. Pregnancy rate was significantly higher in bitches inseminated twice with fresh semen than in those inseminated once only. Litter size and gestation length increased with the number of AIs. Most bitches whelped 62 days after a single AI with fresh or frozen semen, but the gestation period varied between 55 and 69 days. There were significant differences in fertility between breeds. Pup deaths occurred in 33.8% of the litters and the pup death rate during the first 3 weeks of life was 11.6%. The number of pups resulting from AI amounted to 1.1% of the total number of pups registered with the Swedish Kennel Club.

Animals↗
PubMed1993

Variation in spontaneous abortion rate relates to the indication for therapeutic donor insemination.

OBJECTIVE: To evaluate the relationship between indications for donor insemination and the rate of spontaneous abortion of these pregnancies. METHODS: All therapeutic donor insemination pregnancies from our clinic over a 15-year period were categorized according to the indications for insemination, recipient's age, cycle of conception, use of ovulation-inducing agents, use of fresh or frozen semen, and pregnancy outcome. Spontaneous abortion rates were compared between groups segregated according to the indication for donor insemination; differences in maternal age were taken into account. Statistical evaluation was done using chi 2 analysis. RESULTS: Women whose partners produced some sperm in their ejaculates had a spontaneous abortion rate of 21.8%, as compared to 15.4% for women whose partners were azoospermic (P < .05). When couples with oligospermia were segregated by degree of oligospermia, those with counts greater than 2 x 10(6)/mL had an abortion rate of 26.2%, as compared to 11.7% in those with counts less than 1 x 10(6)/mL (P < .025). CONCLUSIONS: The indication for performing therapeutic donor insemination has an influence on the resultant spontaneous abortion rate. Several possible reasons for this relationship may exist, but more data will be needed for verification. A better understanding of the influences that the indication for donor insemination has upon the outcome will facilitate more accurate counseling of candidate couples.

Abortion, Spontaneous↗
PubMed1996

Effect of sperm dose on pregnancy rate from intrauterine insemination: a retrospective analysis.

Both the number of motile spermatozoa inseminated and the site of insemination have been correlated with the probability of pregnancy in patients inseminated with donor sperm cells from fertile men. Nevertheless, more data on the minimum sperm dose required to achieve a pregnancy are needed to understand this apparent relationship. We analyzed retrospectively 2280 cycles of intrauterine insemination to test the hypothesis that intrauterine insemination requires a minimum number of motile sperm cells to maximize the pregnancy rate. Our analysis of 1761 cycles of intrauterine insemination using from 200,000 to more than 200 million motile sperm cells showed no significant relationship between sperm dose and pregnancy rate.

Adult↗
PubMed1996

Reproductive and financial impact of a do-it-yourself artificial insemination programme compared with keeping a bull.

The fertility of five dairy herds was studied for three years while they initiated a programme of do-it-yourself artificial insemination (DIY AI) and kept a herd bull, and the financial impact of the new programme was assessed. The herds of approximately 100 cows were all Friesian pedigree, changing to Friesian/Holstein. The DIY inseminators each operated within one herd and were all trained at the same centre. Within a herd, each cow inseminated by a bull was paired with a cow inseminated by AI. The paired cows calved during the same week and were of the same lactation number and genetic type. Each year 20 matched pairs, that is 40 cows per herd, were used, giving a total of 600 cow records. During the first two years, in the majority of herds the calving interval decreased in both the cows inseminated by DIY AI and by the bull. There was a higher culling rate for all cows in the first year, resulting in an improved Fertex score and improved profitability in the second year compared with the first. However, in the five herds combined it was only by the third year that the calving interval for the DIY AI cows was shorter (by 17.9 days) than for cows inseminated by the bull (P < 0.05). The first year of the DIY AI programme was a financial strain for the farmers, but the second and third years showed enough improvement to recover the losses incurred in initiating DIY AI, that is, the capital investment and learning period.

Animal Husbandry↗
PubMed1996

Effect of timing of frozen semen insemination on pregnancy rate in mares.

Thirty-four mares were inseminated with frozen semen from one stallion during 2 oestrous cycles, every 48 h until ovulation took place and within 12 h after ovulation. Semen was frozen using the Colorado method. The insemination dose was from 200 to 400 x 10(6) progressively motile spermatozoa. Ovaries were examined every 12 h to determine time of ovulation. Examination for pregnancy was carried out using ultrasonography, 15 days after ovulation. Thirty-five per cent of mares inseminated < 24 h and 23% of mares inseminated between 24-48 h before ovulation were pregnant (p = 0.388). The pregnancy rate in all mares inseminated before ovulation was 30%. In the mares inseminated within 12 h of ovulation, it was 18% (p = 0.253). Younger mares (aged 4-10 yr) had a higher pregnancy rate (59%) than older mares (aged 11-15 yr) (23%), but the difference was not statistically significant (p = 0.057).

Animals↗