Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Histocompatibility Testing”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 415 records · Page 23Linked to original sources
PubMed1992

One year's experience with two different image analysis systems for automated reading of the contrast fluorescence test.

We have tested two different personal-computer-based color image analysis systems for automated reading of the microlymphocytotoxicity test (LCT) for HLA-A,B,C typing and screening. Over 17,000 single LCT reactions were prepared using the simultaneous double fluorescent variant of the LCT (contrast fluorescence test, CFT). All tests were read visually by experienced laboratory staff members. For digital image analysis, an automated scanning system was used. The reactions were first recorded on a videotape recorder using a color (CCD) videocamera und subsequently analyzed with the two different image analysis systems by specifically developed programs. Good correlation (r = 0.89) of the score values assigned by digital image analysis with the visual tray reading was obtained. Since also the other main performance characteristics of the prototype system were acceptable for routine application, we may conclude that digital image analysis is a feasible and very interesting new technique for automated evaluation of the LCT.

Algorithms↗
PubMed1990

Impact of RLA matching and cyclosporine on long-term survival in transplanted rabbits.

In order to measure the impact on long-term survival of rabbit leukocyte antigen (RLA) matching and the administration of cyclosporine (CSP) in allogeneic marrow transplantation of irradiated adult rabbits, four groups of five rabbits each were studied. Group 1 consisted of RLA-mismatched animals that did not receive CSP following transplant. All five animals died of infectious complications or graft-versus-host disease (GVHD) (median survival 12 days, range 6-20 days). Group 2 consisted of animals that were similarly mismatched but treated with CSP as postgrafting immunosuppression. All animals also died of GVHD or infection but the median survival was extended to 18 days, range 12-27 days. Group 3 consisted of RLA-matched animals that did not receive CSP. Median survival was 65 days, range 27-121 days, and two are still alive at 120 and 121 days after transplant. Three of five animals died of GVHD. The only group of animals to consistently achieve long-term survival was group 4; these animals were RLA matched and were treated with CSP. Five of five animals survived with a median survival of 564 days following transplant, range 220-806 days. All five became complete hematopoietic chimeras as determined by cytogenetic analysis of bone marrow lymphocytes. Thus, long-term survival after allogeneic bone marrow transplantation in the irradiated adult rabbit was achieved and was dependent upon the use of RLA-matched donors and the administration of cyclosporine as GVHD prophylaxis.

Animals↗
PubMed1988

The B-G region genes of the chicken MHC are responsible for lethal graft-versus-host disease in newly hatched chickens.

Using the genetic model of Prague recombinant congenic lines of chickens we found that incompatibility in the B-G region of the major histocompatibility complex (MHC) causes very severe graft-versus-host reaction (GVHR)-associated haemolytic anaemia in newly hatched chickens. Unexpectedly, mild or no signs of this GVH disease are elicited when a recipient chick and an adult donor of lymphocytes are incompatible in the whole B haplotype (B-F/L + B-G regions). On the other hand, the B-G region incompatibility alone (as has been described previously) is not sufficient to produce any GVH splenomegaly in embryos at 14 days of incubation. However, GVH splenomegaly in the donor-recipient combinations with the difference in the whole B haplotype (B-F/L + B-G regions) is significantly greater than in those with the B-F/L region difference only. These results confirm that the B-G region genes of chicken MHC are also involved in the histocompatibility reactions. Furthermore, a new hypothetical model for the structure of the chicken MHC is discussed.

Anemia, Hemolytic, Autoimmune↗