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PubMed1995

Receptor specificity of influenza virus influences severity of illness in ferrets.

Weanling ferrets were inoculated intranasally with either wild-type or receptor-variant clones of influenza A/Memphis/102/72 to determine if changes in receptor specificity influence virulence of influenza virus infection. Over the 5 days after inoculation, receptor-variant inoculated ferrets had a lower mean elevation in body temperature, greater weight gain and less sneezing than the wild-type group. Influenza virus was recovered from the lungs of fewer receptor-variant infected ferrets (5/12 vs 11/12) and in lower titers than in wild-type infected ferrets at 5 days after inoculation. The viruses recovered from lung homogenates retained the same receptor specificity as the inoculum. Serum hemagglutination inhibition titers for the two groups were similar. These findings suggest that the receptor-variant clone is less virulent but elicits a similar immunogenic response compared with the wild-type clone.

Animals↗
PubMed1984

In vitro activation of the promutagens 2-acetamidofluorene, cyclophosphamide and 7,12-dimethylbenzanthracene by constitutive ferret and rat hepatic S-9 fractions.

The ability of the ferret to metabolically activate promutagenic compounds was compared with that of the rat, using the Salmonella/microsome assay. Three compounds which require biotransformation to mutagenic metabolites, 2-acetamidofluorene (2-AAF), cyclophosphamide (CPA), and 7,12-dimethylbenzanthracene (DMBA), were studied. Metabolic activation was provided by ferret or rat hepatic S-9 fractions at 5 levels for each chemical, and optimal S-9 levels as well as dose-response curves were obtained. Interspecies mutagenic activity was quantitated on the basis of mg liver, mg S-9 protein, and nmoles P-450. The slopes of the dose-response curves and the lowest chemical dose required for a significant response were also compared. Although constitutive levels of rat hepatic cytochrome P-450 were shown to be higher than those of the ferret, in vitro mutagenic activation by ferret S-9, at S-9 levels which caused activation in both species, was greater than or equivalent to activation by rat S-9 for these chemicals, based on all parameters studied. The results showed that the equilibrium between activation and detoxification reactions is dependent upon the chemical dose and S-9 level present.

2-Acetylaminofluorene↗
PubMed1991

Testosterone in MPOA elicits behavioral but not neuroendocrine responses in ferrets.

The amount of time male ferrets were engaged in neck gripping, mounting, and thrusting was quantified in 30-min tests with a receptive female before and after castration. Bilateral cannulae containing a total of approximately 2 mg testosterone propionate (TP) in cocoa butter were then stereotaxically aimed at the medial preoptic area (MPOA). Tests for sexual behavior were conducted on days 3, 7, 14, and 21 postimplantation. Ferrets were histologically categorized as either 1) Miss (implants not in MPOA), 2) Unilateral implant in MPOA, or 3) Bilateral implants in MPOA. The mean amount of time spent neck gripping, mounting, and thrusting increased significantly over castrate levels on postimplantation day 14 in the Bilateral group, but not in the Miss or Unilateral groups. In all groups, mean plasma testosterone concentrations were below or near the lower limit of detectability on the day before TP implantation and on postimplantation test days. In the same plasma samples, luteinizing hormone concentrations were within the normal range of castrated ferrets, and did not significantly decline after TP implantation. These results suggest that the MPOA is a neural site for androgen activation of certain components of reproductive behavior but not for negative feedback on gonadotropin secretion in male ferrets.

Animals↗
PubMed1993

A sex comparison of serotonin immunoreactivity and content in the ferret preoptic area/anterior hypothalamus.

Previous studies with rats raised the possibility that sexually dimorphic features of the medial preoptic area/anterior hypothalamus (POA/AH) may result, in part, from a sex difference in the serotonergic innervation of this region. We asked whether a similar phenomenon may occur in a carnivore, the ferret. A sexually dimorphic male nucleus of the dorsal POA/AH (Mn-POA/AH) has previously been characterized in Nissl-stained sections of the male ferret forebrain; this nucleus is absent in females. A nondimorphic ventral nucleus of the POA/AH is found in both sexes. In the present study numerous serotonin (5-HT) immunoreactive (ir) fibers were observed in the dorsal POA/AH of gonadectomized adult ferrets of both sexes. By contrast, in both sexes the ventral nucleus of the POA/AH had many fewer 5-HTir fibers. A similar difference in the distribution of immunoreactivity between dorsal and ventral POA/AH was observed for tyrosine hydroxylase (TH) localized in cell bodies and in nerve fibers and for H222ir estrogen receptors localized in cell nuclei. Likewise, in both sexes the content of 5-HT and dopamine (DA), measured by high pressure liquid chromatography with electrochemical detection, were significantly higher in the dorsal than the ventral POA/AH, thereby corroborating observed regional differences in 5-HTir and THir fibers, respectively. The present findings provide no support for the notion that sexually dimorphic cytoarchitectonic features of the dorsal POA/AH in ferrets are associated with a sex difference in the serotonergic innervation of this region.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1994

The connectivity of the area postrema in the ferret.

The area postrema (AP) is the chemosensitive trigger zone for the emetic reflex. We have investigated the connectivity of the AP and adjacent solitary complex (SC) to identify possible sites of the motor emetic center. The AP and SC were infused with HRP or WGA-HRP in 30 ferrets that were perfused transcardially after 24-72 h. A block from the pons to upper cervical spinal cord, and one with hypothalamus and basal forebrain, was cut at 50 microns, reacted, and mounted. Data support the conclusion, at variance with those from other preparations, that in ferrets the AP has reciprocal connections only with the SC, which serves as a relay in both ascending and descending pathways between AP and higher levels of the neuraxis. Connectivity of the SC with brain stem and forebrain structures including the rostral ventrolateral medulla, parabrachial nuclei, paraventricular nucleus, and amygdala was demonstrated. At least in ferrets, our results suggest that the motor emetic center must be located within the SC. While this may not apply to all species, it is also possible that some reports of AP projections elsewhere were results of label within the SC. Alternatively, the somewhat different pattern of emesis in the ferret as compared to the dog (greater role for vagal inputs in response to radiation and cytotoxic drugs, lesser role for humoral inputs) may reflect differences in AP connectivity.

Animals↗
PubMed1988

An anomaly in the auditory brain stem projections of hypopigmented ferrets.

The number of cochlear nucleus (CN) neurones projecting to the ipsi- and contralateral inferior colliculus (IC) was studied in darkly pigmented and hypopigmented ferrets by the retrograde transport of wheat germ agglutinin-horseradish peroxidase. The contralateral projection in all divisions of the CN of both albino and 'red eyed' ferrets was normal. However, the ipsilateral projection from the anteroventral CN was significantly smaller in albino than in pigmented ferrets. The volume of each division of the CN was also measured and was found to be the same in albinos as in pigmented animals. The results show that hypopigmented ferrets have at least one anomaly among the connections of the auditory brain stem.

Albinism↗
PubMed1989

Brainstem auditory evoked response in the ferret (Mustela putorius).

The effect of click intensity, repetition rate and binaural interaction on the brainstem auditory evoked response (BAER) was examined in sixteen pigmented adult male ferrets. Potentials were recorded from platinum needle electrodes inserted over the vertex and left and right mastoids. Square waves, 100 microseconds in duration, were transduced by earphones enclosed in an assembly designed to fit securely over the ferret's external ear. The BAER in the ferret consists of four prominent vertex-positive peaks (P1-P4) and a fifth peak of smaller amplitude and more variable latency. The mean latencies of P1-P4 at 104 dB peak SPL were 0.96, 1.83, 2.75 and 3.62 ms. Reducing intensity over a 70 dB range resulted in a reduction in amplitude and a corresponding increase in latency ranging between 0.57 and 0.67 ms. Also, increasing click repetition rate resulted in a reduction in amplitude and an increase in latency. With intensity fixed at 104 dB peak SPL comparison of latencies at 10 and 50/s showed a mean increase of 20, 50, 60 and 80 microseconds for P1-P4, respectively. The effect of binaural interaction on the BAER was examined using the procedure of Dobie and Berlin (1979); the response evoked by binaural stimulation was subtracted from the summed left and right monaural responses to obtain a binaural interaction component. Binaural interaction in the ferret gave rise to a distinct vertex-negative wave with a latency similar to P4. An increase in click intensity over a 70 dB range resulted in a monotonic increase in amplitude and a decrease in latency of the binaural interaction component.

Acoustic Stimulation↗
PubMed2005

Histological and histochemical observations on salivary microliths in ferret.

The fortuitous observation of salivary microliths in ferret was pursued in the present investigation. Major salivary glands obtained post-mortem from mature ferrets of either sex were examined with the use of histology and light microscopical histochemistry for calcium, protein, amino acids, mucosubstances and hydrolytic enzymes. Microliths were detected in most parotids, but were absent from submandibular and sublingual glands. The microliths were usually seen in lumens, and occasionally in parenchyma and interstices. They were variably stained for calcium, tryptophan, and neutral and acidic mucosubstances, similarly to acinar or ductal secretory granules. Unlike secretory granules, microliths showed autofluorescence, high levels of tyrosine and a low concentration of -SS- groups. Acid phosphatase and beta-glucuronidase reaction surrounded non-luminal microliths. The present data establish ferret as a new model for the investigation of salivary microliths and do not support the notion of microliths being almost absent from the parotid. Probably there is secretory inactivity in ferret parotid and this fosters the formation and accumulation of microliths containing calcium and disintegrated secretory material.

Acid Phosphatase↗
PubMed2004

Morphology of ferret subcutaneous adipose tissue after 6-month daily supplementation with oral beta-carotene.

Adipose tissue is an important retinoid depot and retinoids are known to influence white and brown adipocyte metabolism. Identifying nutrients that can affect the biological activity of the adipose organ would be of great medical interest in the light of the current obesity epidemic and related disorders in developed countries. The vast majority of mammal studies of chronic administration of oral beta-carotene have used murine models, while few have employed mammals exhibiting uptake and processing of intestinal beta-carotene similar to those of humans. While rodents transform practically all ingested beta-carotene into retinol, in ferrets, as in humans, part of the beta-carotene is absorbed and released into the circulation intact. We studied the effects of 6-month daily administration of two doses of oral beta-carotene (0.8 or 3.2 mg/kg/day) on ferret body weight, size of body fat depots, and, using morphological and morphometric methods, on subcutaneous (inguinal) white adipose tissue (WAT). Because of the oral mode of administration, liver, stomach, and small and large intestine were also studied. Control animals received the vehicle. Data show that at the end of treatment the higher dose induced significantly higher body weight compared with controls and significantly higher inguinal fat depot compared with animals treated with the lower dose. In addition, chronic treatment with beta-carotene induced a dose-dependent hypertrophy of white adipocytes and increased neoangiogenesis in subcutaneous WAT in all treated ferrets. Vasculogenesis was independent of adipocyte hypertrophy. We also found focally evident liver steatosis in the ferrets treated with the higher dose of beta-carotene. The other gastrointestinal tract organs studied were not significantly different from those of control animals.

Adipose Tissue↗
PubMed2006

Hepatic hemangiosarcoma in two ferrets (Mustela putorius furo).

Two ferrets were presented to the authors' clinic. Hemoperitoneum was diagnosed in one ferret, and an abdominal mass was palpated in the other. One ferret was euthanized and necropsied, and one ferret underwent exploratory laparotomy and liver lobectomy. In both cases, the histopathologic diagnosis was hepatic hemangiosarcoma.

Animals↗
PubMed2006

Pacemaker implantation in a ferret (Mustela putorius furo) with third-degree atrioventricular block.

A 7.5-year-old castrated male ferret (Mustela putorius furo) was diagnosed with third-degree atrioventricular (AV) block. A monopolar epicardial pacemaker system was implanted, resulting in a regular, paced cardiac rhythm with third-degree AV block at 140 beats per minute. Over the next 2 months, the ferret developed anorexia, interstitial pneumonia, intermittent diarrhea, and hind-limb weakness and had a slow and progressive recovery. The ferret developed clinical signs of congestive heart failure 4 months after the surgery, resulting in its death 3 weeks later. Necropsy results attributed the death to cardiac failure due to extensive myocardial mineralization. To the authors' knowledge this is the first published report of surgical report of surgical pacemaker implantation in a ferret.

Animals↗
PubMed2006

Comparison of odor and mating-induced glomerular activation in the main olfactory bulb of estrous female ferrets.

Previously [S.K. Woodley, M.J. Baum, Differential activation of glomeruli in the ferret's main olfactory bulb by anal scent gland odors from males and females: an early step in mate identification, Eur. J. Neurosci. 20 (2004) 1025-1032], the receipt of intromission from a male activated glomeruli (indexed by Fos immunoreactivity in juxtaglomerular cells) in the main olfactory bulb (MOB) of estrous female ferrets which exceeded the activation seen after exposure to male anal scent gland odorants alone. We asked whether centrifugal inputs (e.g., from the locus coeruleus to the MOB) generated by the receipt of vaginal-cervical stimulation influence odor-induced MOB glomerular activation. We compared the activation of MOB glomeruli in estrous female ferrets which received a unilateral naris occlusion prior to exposure to: unscented air, volatile odorants from an anesthetized male, volatile + non-volatile odorants from direct physical contact with an anesthetized male, or mating stimulation. Little glomerular activation was observed in the MOB ipsilateral to an occluded naris, including females which received intromission. An equivalent distribution of activated glomeruli was observed in the ventral MOB of estrous females which either received mating stimulation or had direct physical contact with an anesthetized male. Considerably less glomerular activation occurred in females exposed only to volatile male odors. The MOB of female ferrets responded to body odorants from the opposite sex; however, there was no evidence that mating-induced centrifugal inputs directly activated MOB glomeruli or modified odor-induced glomerular activation.

Action Potentials↗
PubMed2005

Unilateral cochlear ablation in adult ferrets results in upregulation in calretinin immunostaining in the central nucleus of the inferior colliculus.

In the present study, unilateral cochlear ablations were performed in adult ferrets in order to determine whether an upregulation of the calretinin immunostained plexus in the central nucleus of the inferior colliculus occurs and if so, what the time course of this upregulation is. Accordingly, the mean gray level and the calretinin-immunostained area of the axonal plexus in the central nucleus of the inferior colliculus were evaluated at 1, 20 and 90 days after cochlear ablation. In unoperated animals, the calretinin-immunostained plexus was bilaterally symmetric. In ablated animals, both the mean gray level and the immunostained area of the plexus increased in the central nucleus of the inferior colliculus contralateral to the lesion compared with both the ipsilateral side and unoperated animals. This upregulation was present 24 h after the ablation and did not change at the two subsequent time points. In a previous study in young ferrets, the immunostained area of the plexus in the central nucleus of the inferior colliculus contralateral to the lesion increased 200% compared with control ferrets [J Comp Neurol 460 (2003) 585], whereas it increased only 33% in adult ferrets. These findings suggest that 1) calretinin upregulation in the contralateral central nucleus of the inferior colliculus following cochlear ablation occurs by 24 h after cochlear ablation and 2) there is an age-related decline in the magnitude of this upregulation after cochlear ablation.

Animals↗
PubMed2004

Stages and duration of spermatogenesis in the domestic ferret (Mustela putorius furo).

Classification of seminiferous tubules is the basis for understanding normal and abnormal spermatogenesis. The aim of the present study was to determine spermatogenic stages and the duration of the cycle in the domestic ferret using bromodeoxyuridine (BrdU) as a tracer. Eleven adult male ferrets that were maintained in a breeding condition were used. Testicular sections were stained with the periodic acid-Schiff reaction for light microscopy. To determine the cycle duration, six ferrets were injected intraperitoneally with BrdU, and testes were collected 3h later and 10 days and 3h later. BrdU was detected by immunohistochemistry. Seminiferous tubules were classified into eight stages, and frequencies of stages I-VIII were 10.6, 2.2, 7.9, 13.1, 22.3, 21.9, 14.0 and 8.0%, respectively. The most advanced BrdU-labeled cells at 3h post-injection were leptotene spermatocytes in stage VI and those at 10 days and 3h were pachytene spermatocytes in stage V. From differences in stage frequency and BrdU staining frequency between two time points, the duration of one cycle was estimated to be 13.0 days. The present observations indicate that stages and the cycle duration of the ferret spermatogenesis are similar to those reported in other carnivores.

Animals↗
PubMed2005

Evaluation of modified vaccinia virus Ankara based recombinant SARS vaccine in ferrets.

Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) remains a threat to cause epidemics as evidenced by recent sporadic cases in China. In this communication, we evaluated the efficacy and safety of two SARS vaccine candidates based on the recombinant modified vaccinia Ankara (MVA) expressing SARS-CoV spike or nucleocapsid proteins in ferrets. No clinical signs were observed in all the ferrets challenged with SARS-CoV. On the other hand, vaccination did not prevent SARS-CoV infection in ferrets. In contrast, immunized ferrets (particularly those immunized with rMVA-spike) exhibited significantly stronger inflammatory responses and focal necrosis in liver tissue after SARS-CoV challenge than control animals. Thus, our data suggest that enhanced hepatitis is linked to vaccination with rMVA expressing SARS-CoV antigens.

Animals↗
PubMed2005

Prevention of disease in ferrets fed an inactivated whole cell Campylobacter jejuni vaccine.

Ferrets were used to demonstrate the potential of a killed whole cell vaccine prepared from Campylobacter jejuni to protect against disease. C. jejuni strain 81-176 was grown in BHI broth, formalin-fixed, and resuspended in PBS to a concentration of 10(10) cells per ml. This vaccine (CWC) or live organisms were delivered orally with a nasogastric tube into anesthetized animals treated to reduce gastric acidity and intestinal motility. When 5x10(10) CFU of the vaccine strain (Lior serotype 5) or one of two other serotypes, CGL-7 (Lior 4) or BT44 (Lior 9), was used to challenge the ferrets, all of the animals developed a mucoid diarrhea. If the animals had been challenged with 5x10(9) CFU of the homologous strain 1 month before challenge with 10(10) CFU, 80-100% protection against disease was seen. This protection was also obtained after an initial exposure to the 81-176 strain followed by challenge with either of the heterologous strains. CWC was used to see if protection demonstrated with the live organisms could be produced with the non-living preparation. When 10(9) cells of CWC was given as two doses 7 days apart with or without 25mug of a coadministered mucosal adjuvant, LT(R192G), only 40-60% of the animals were protected. If the regimen was changed to four doses given 48h apart, 80% of the animals were free of diarrhea after subsequent challenge. Increasing the number of cells in the four dose regimen to 10(10) cells did not improve protection. Animals given four doses of 10(10) cells combined with LT(R192G) were subsequently challenged with 10(10) cells of the homologous strain or the heterologous strain CGL-7. The CWC protected against both strains. Serum IgG antibody titers determined by ELISA showed little increase following the CWC four dose vaccination regimen, compared to animals given one dose of the live organism. On subsequent challenge, however, both CWC vaccinated and live-challenged ferrets showed comparable antibody titer increases above those obtained following the initial challenge or vaccination. Western blots were used to show that the immunodominant antigen in vaccinated animals was a 45kDa protein, while in ferrets challenged with live organisms the immunodominant antigen was a 62kDa protein. These data show that the CWC can be used to protect against disease caused by Campylobacter. They also show that protection and serum IgG responses do not depend upon the use of the mucosal adjuvant and that cross protection among some of the major serotypes of Campylobacter responsible for human disease is possible.

Animals↗
PubMed2005

Studies on vertical transmission of Trichinella spp. in experimentally infected ferrets (Mustela putorius furo), foxes (Vulpes vulpes), pigs, guinea pigs and mice.

Vertical transmission of Trichinella spiralis was evaluated in ferrets (n=21), foxes (n=11), pigs (n=12), guinea pigs (n=16), and mice (n=41). The placental barrier to be crossed by migratory Trichinella larvae varies structurally in different animal species. Ferrets and foxes have an endotheliochorial placenta structure, guinea pigs and mice a haemochorial, and pigs an epitheliochorial placenta. The non-encapsulating Trichinella pseudospiralis larvae have an extended muscle migration prior to entering a muscle cell. To evaluate if T. pseudospiralis was more likely to be transmitted to offspring, an additional group of foxes (n=11) infected with T. pseudospiralis was included. Two different dose levels were used for ferrets, pigs, guinea pigs, and mice. In pigs and guinea pigs, infection was given at different times of the gestation period. Vertical transmission, measured as recovery of muscle larvae in the offspring, was demonstrated in both ferrets groups, in all four guinea pig groups, and in the high dose mouse group, but not in any fox or pig groups.

Animals↗
PubMed2006

Motion perception deficits in albino ferrets (Mustela putorius furo).

Albino ferrets contrary to their pigmented conspecifics show no optokinetic nystagmus. Therefore, in this study motion perception was compared between pigmented and albino ferrets (Mustela putorius furo) trained to discriminate between coherently moving random dot patterns and dynamic noise stimuli in a two-alternative forced choice task. Fully coherently versus incoherently moving patterns could be distinguished by ferrets of both phenotypes. Motion coherence thresholds, however, were significantly higher in albinos. These results indicate that albino ferrets are not motion blind as could be expected from their total lack of optokinetic reactions. However, they are severely impaired in global motion perception.

Albinism, Ocular↗