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New trends in dentin/enamel adhesion.

The acid-etch-technique has provided an ideal surface for bonding to enamel by using 30-40% phosphoric acid. The resulting etch pattern is characterized by the profuse formation of microporosities which allow the penetration of monomers into those porosities to form resin tags that provide micromechanical retention. Successful attempts of bonding to dentin in a similar fashion have been reported more recently. Due to the specific properties of dentin, such as its tubular structure and its intrinsic wetness, bonding to dentin has not yet reached the ideal characteristics. In spite of the existing deficiencies in dentin adhesion, the increasing demand for esthetic restorations has generated intensive research on new esthetic materials with special focus on amalgam alternatives. The bonding mechanism of recent dentin bonding agents is based on the penetration of ambiphilic molecules into acid-etched dentin to form a lacework of dentin collagen and polymerized monomers. Dentin adhesive systems that contain a multitude of different bottles of different colors and shapes belong to the past. Because clinicians are increasingly eager to try new materials, the actual tendency calls for simplification of the bonding procedure e.g. one-bottle adhesive systems and all-in-one no-bottle materials. In spite of simpler materials, a separate etching step is still needed for one-bottle systems. Nevertheless, manufacturers of these simplified one-bottle materials recommend their use to bond polyacid-modified composites (compomers) without a separate etching step. The most recent addition to the group of simplified adhesives is the all-in-one no-bottle adhesives; one of these all-in-one systems, Prompt L-Pop (ESPE) has resulted in very promising laboratory results when used on enamel. In spite of the uncertainty about the capacity of all-in-one adhesives to etch enamel adequately in vivo, scanning electron microscopy studies have resulted in an enamel-etching pattern morphologically similar to that corresponding to phosphoric acid-etched enamel. While all-in-one adhesive systems have been reported to result in very satisfactory dentin bond strengths, results from other laboratories suggest that bonding to dentin with all-in-one adhesive systems will need to be somewhat improved. Clinical studies, which are the ultimate test for the acceptance of dentin adhesives, are now underway in several centers. Six-month data showed a very good clinical performance for this ultra-simplified all-in-one adhesive system.

Acid Etching, Dental↗

Adhesive luting of indirect restorations.

PURPOSE: To describe the potential of adhesive luting procedures with respect to (1) material characteristics and classifications, (2) film thickness, (3) overhang control, (4) bonding to different inlay materials, (5) adhesion to tooth substrates and the problem of hypersensitivities, (6) wear of luting composites, and (7) clinical performance. MATERIALS AND METHODS: A literature review of relevant studies of various in vitro and in vivo studies enables an overview of possibilities and limitations of adhesively luted indirect restorations. RESULTS: (1) Resin-based composites are the material of choice for adhesive luting. Both material properties and wear behavior of fine particle hybrid-type resin-based composites are superior to other materials. The use of compomers is questionable due to hygroscopic expansion and possible crack formation as proven for IPS Empress caps in vitro and in vivo. (2) Recent luting cements exhibit excellent flow characteristics with mean film thicknesses ranging between 8 microm and 21 microm. The ultrasonic insertion technique is recommended for viscous luting composites or conventional restorative composites utilizing their thixotropic properties. (3) For successful overhang control, good fit of the restoration (during luting) and high radiopacity of the cement (after luting) are indispensable. Overhang control is estimated easier when the ultrasonic insertion technique is applied. (4) The pre-treatments of ceramic inlays using hydrofluoric acid or silica coating result in effective bonding; for pre-treatment of resin-based composite inlays, silica coating is promising as well. (5) Bonding to enamel and dentin is proven clinically acceptable, but it should be performed with multi-step systems providing separate primers and bonding agents producing a perfect internal seal with almost no hypersensitivities. Dual-cured multi-step bonding agents provide the most promising potential. (6) The viscosity and filler content of the resin composite used for luting does not influence the wear characteristics within the marginal luting area in vivo. However, the ultrasonic insertion technique involving high viscosity materials provides enhanced handling characteristics for luting of tooth-colored inlays. (7) Clinical results with tooth-colored inlays and veneers are promising over periods of up to 10 yrs, including use in severely destroyed teeth.

Acid Etching, Dental↗

Marginal integrity after fatigue loading of ceramic inlay restorations luted with three different cements.

PURPOSE: To evaluate the effect of fatigue loading on the quality of the cement around the margins of adhesive ceramic MOD inlays. MATERIALS AND METHODS: Non-metal IPS Corum porcelain inlays were made and cemented either with the resin-based composite Variolink, the compomer Dyract-Cem or the glass-ionomer cement Ketac-Cem. For each system, half of the specimens were subjected to thermal and mechanical load cycling to simulate fatigue loading. All teeth were then sectioned and evaluated by SEM on topography and by optical microscopy for dye penetration. RESULTS: Before loading, all systems showed good marginal integrity. After loading, inlays cemented with Variolink and Dyract-Cem showed no significant (P > 0.05) increase in marginal break down, however restorations cemented with Ketac-Cem, showed a significant (P < 0.05) deterioration at the margins.

Cementation↗

Orthodontic-restorative treatment of chipped or worn incisors.

PURPOSE: To evaluate the shear bond strength of esthetic adhesive restorations for worn or fractured incisal edges. MATERIALS AND METHODS: On 30 human extracted incisors the edge was reduced and a bevel was prepared in the thickness of enamel. Then, the incisal edges were restored, using a different adhesive system in each of the three groups to which the sample teeth had been randomly assigned. Group A: Scotchbond Multi-Purpose Plus, in combination with phosphoric acid, and Z100 as a restorative material. Group B: Scotchbond 1 after etching with phosphoric acid, plus Z100. Group C: F2000, a compomer, in combination with its Primer/Adhesive. The differences in the bond strength values among the three groups were tested for statistical significance. Also, the resistance to load of the restored teeth was compared with that of 10 intact incisors (Group D) (controls). A statistical analysis was performed to test the significance of the differences. RESULTS: No statistically significant difference in shear bond strength was found between Scotchbond Multi-Purpose Plus (17.6 MPa) and Scotchbond 1 (14.8 MPa), whereas F2000 gave significantly lower bond strength values (10.1 MPa). As compared with intact incisors, the teeth restored with either Scotchbond Multi-Purpose Plus or Scotchbond 1 did not perform significantly differently under load. On the other hand, the incisors restored with F2000 gave, as compared with the controls, a resistance to loads significantly lower and of a questionable reliability from a clinical standpoint. SEM observations performed on some of the samples revealed that when phosphoric acid was used to condition the substrate, a deep and uniform demineralization of the enamel was effected, and a mechanically retentive interlocking was created at the interface between resin and enamel. The Adhesive/Primer of F2000 was unable to produce an equally retentive morphology.

Acid Etching, Dental↗

Effect of prophylactic polishing protocols on the surface roughness of esthetic restorative materials.

Many polishing protocols have been evaluated in vitro for their effect on the surface roughness of restorative materials. These results have been useful in establishing protocols for in vivo application. However, limited research has focused on the subsequent care and maintenance of esthetic restorations following their placement. This investigation evaluated the effect of five polishing protocols that could be implemented at recall on the surface roughness of five direct esthetic restorative materials. Specimens (n=25) measuring 8 mm diameter x 3 mm thick were fabricated in an acrylic mold using five light-cured resin-based materials (hybrid composite, microfilled composite, packable composite, compomer and resin-modified glass ionomer). After photopolymerization, all specimens were polished with Sof-Lex Disks to produce an initial (baseline) surface finish. All specimens were then polished with one of five prophylactic protocols (Butler medium paste, Butler coarse paste, OneGloss, SuperBuff or OneGloss & SuperBuff). The average surface roughness of each treated specimen was determined from three measurements with a profilometer (Surface 1). Next, all specimens were brushed 60,000 times at 1.5 Hz using a brush-head force of 2 N on a Manly V-8 cross-brushing machine in a 50:50 (w/w) slurry of toothpaste and water. The surface roughness of each specimen was measured after brushing (Surface 2) followed by re-polishing with one of five protocols, then final surface roughness values were determined (Surface 3). The data were analyzed using repeated measures ANOVA. Significant differences (p=0.05) in surface roughness were observed among restorative materials and polishing protocols. The microfilled and hybrid resin composite yielded significantly rougher surfaces than the other three materials following tooth brushing. Prophylactic polishing protocols can be used to restore a smooth surface on resin-based esthetic restorative materials following simulated tooth brushing.

Analysis of Variance↗

Amalgam repair: evaluation of bond strength and microleakage.

This study evaluated the tensile bond strength of "repaired" amalgams and compared the degree of microleakage. Amalgam (Cavex avalloy) was condensed into plastic tubes (3 mm in diameter, 10 mm in height) to the half-length. After storage in water at 37 degrees C for two days, the remaining parts of tubes were filled with amalgam (A), cavity varnish (CV)+A, Liner Bond 2V (LB2V)+A, 3M Opal Luting Cement (3MOLC)+A, Panavia F(PF)+A, Metabond(MB)+A, Fuji BondLC(FB)+A, HytacOSB(HOSB)+Hytac Aplitip (H), Liner Bond2V+Clearfil AP-X(CAP). The bond strengths for 15 samples of each restoration group were determined. For the microleakage study, MOD cavities of 90 extracted human premolars were used. The distal half of cavities were filled with amalgam. After storage in water at 37 degrees C for two days, the mesial half of the cavities were filled to simulate a clinical repair. The "repair" was placed using the procedures applied in the bond strength study. The teeth were stained with basic fuchsine (0.5%), sectioned and evaluated for dye penetration. In both parts of study, the data were analyzed by ANOVA and Duncan's multiple range tests. Bond strength values (MPa) were: A+PF+A 3.84+/-1.08, A+LB2V+A 3.15+/-0.97, A+LB2V+CAP 3.05+/-0.53, A+MB+A 2.86+/-0.88, A+HOSB+H 2.58+/-0.51, A+3MOLC+A 2.11+/-0.75, A+FB+A 0.68+/-0.59. The repaired A+A and A+CV+A groups were separated before testing. The A+PF+A group showed the highest bond strength (p<0.05). Microleakage in the cervical margins of repaired restorations was lower in the amalgam groups than microleakage in the resin composite and compomer groups. PF, MB, 3MOLC and FB performed better at the amalgam "repair" interface. The A+LB2V+A group showed no microleakage at both the occlusal and gingival test regions.

Analysis of Variance↗

In vitro efficacy of a one-bottle adhesive system with three restorative materials.

The shear bond strength to dentin of a new single-bottle adhesive with three restorative materials (composite resin, compomer, and amalgam) was evaluated. The results were statistically different (ANOVA, p < 0.05). Groups 1 and 2 were not significantly different from each other, but both were statistically higher than Group 3. The fracture mode was not related to the shear bond strength values.

Analysis of Variance↗

Effect of four intermediate layer treatments on microleakage of Class II composite restorations.

This in vitro study examines the marginal sealing ability of four different intermediate materials applied before placement of a condensable composite. Class II preparations were made with gingival margins placed 1.0 mm apical to the cementoenamel junction of 60 extracted teeth, randomly assigned to five groups of 12. Following restoration, teeth were thermocycled, soaked in 0.5% basic fuchsin, and sectioned longitudinally. The resin-modified glass ionomer cement demonstrated significantly less microleakage than the use of a dentin bonding agent alone or in combination with flowable composite, flowable compomer, or autoploymerizing composite (p < 0.05, Dunn's test). This study supports the use of the glass ionomer open sandwich technique in deep Class II direct composite restorations.

Compomers↗

Influence of adhesive application duration on dentin bond strength of single-application bonding systems.

This study examined the relationship between the bond agent application duration and the dentin bond strength of several single-application bonding systems. The restorative material/ bonding systems used were Reactmer, with its single-application bonding system Reactmer Bond (RB, Shofu Inc, Kyoto, Japan); Palfique Estelite, with its single-application bonding system One-Up Bond F (OU, Tokuyama Co, Tokyo, Japan) and F2000 Compomer, with its bonding system Primer/Adhesive in Clicker (F2,3M Dental Products, St Paul, MN 55144, USA). Bovine mandibular incisors were mounted in self-curing resin and wet ground with #600 SiC to expose labial dentin. Adhesives were applied for 5, 10, 20, 30 and 60 seconds, and restorative materials were condensed into a Teflon mold (04 x 2 mm) on the dentin and light activated. Fifteen samples per test group were stored in 37 degrees C water for 24 hours, then shear tested at a crosshead speed of 1.0 mm/min. One-way ANOVA followed by Duncan test (p = 0.05) was done. SEM observations of the treated dentin surface were also conducted. The dentin bond strength ranged from 6.9 +/- 2.4 to 11.2 +/- 2.8 MPa for RB, 8.9 +/- 2.2 to 12.2 +/- 1.9 MPa for OU, and 7.8 +/- 3.1 to 11.4 +/- 2.6 MPa for F2. No significant differences were found among the 10-60 second application duration groups for the systems used. From the SEM observations, demineralization of the dentin surface was more pronounced with longer application duration. The data suggest that the duration of single-application bonding systems was not a crucial factor for determining dentin bond strength, even though morphological changes were observed on the dentin surface.

Adhesiveness↗

One-year retrospective clinical evaluation of hybrid composite restorations placed in United Kingdom general practices.

OBJECTIVE: The purpose of this study was to assess Pertac II restorations placed in general dental practice. METHOD AND MATERIALS: A total of 86 restorations (14 Class I, 10 Class II, 17 Class III, 5 Class IV, and 40 Class V) using a hybrid composite material (Pertac II) placed in 56 patients (mean age, 39 years) in 5 dental practices in the United Kingdom were assessed after 1 year by a trained evaluator and the dental practitioner who had placed the restoration. All the restorations were assessed for anatomic form, marginal adaptation, surface roughness, sensitivity or discomfort, gingival condition, and the presence or absence of secondary caries. RESULTS: Thirty-five (43%) restorations were placed in load-bearing situations, 9% of which were assessed as being in "heavy" occlusion. Five (6%) of the restorations were lost (all Class V restorations), 4 from premolars and 1 from a molar. Four were in wedge-shaped cavities and 1 small restoration was actually dislodged during examination. The remaining 81 (94%) restorations were found to be intact, with no secondary caries detected. A high percentage of optimal scores were recorded for both anatomic form and surface roughness. CONCLUSION: It is concluded that the evaluation demonstrated satisfactory clinical performance of Pertac II restorations after 1 year of clinical service in the wide variety of clinical situations seen in general dental practice. However, the potential for failure of hybrid composite materials in Class V situations appears high. It may be argued that clinicians who wish to use a resin-based material in a Class V situation should use a compomer or microfilled composite.

Adult↗

In vitro evaluation of fluoride-releasing restorative materials for sealing the root canals of overdenture abutments.

PURPOSE: The purpose of this study was to evaluate the ability of newer fluoride-releasing restorative materials to protect the root surface from acid attack. MATERIALS AND METHODS: The materials used were glass-ionomer cement (GIC), resin-modified glass-ionomer cement (RM-GIC), and a compomer (Comp). A composite resin (CR) was used as the control. The restored teeth were stored in deionized, distilled water for 14 days and subjected to 300 thermocycles (55 degrees C and 5 degrees C). The teeth were cycled in a demineralizing solution (pH 5.0 or 4.0) for 6 hours and in a remineralizing solution (pH 7.0) for 17 hours for 10 days. The depths of lesions created by acid challenge were measured at the interface of the tooth and the restorative material and then at a distance of 50, 100, and 300 microns from the tooth-restoration margin using polarized light microscopy and contact microradiography. RESULTS: At pH 4.0, there was significant difference in the depth at the interface between the tooth and the restorative material (P < .001). The GIC and RM-GIC were protective, and the lesion depths were significantly shallower than for Comp or CR. The protective effect varied depending on the distance from the interface of the tooth and the restorative material. At pH 5.0, the GIC and RM-GIC had no lesions at the interface, while the Comp and the CR had lesions (P < .001). CONCLUSION: Fluoride-releasing glass-ionomer cement seems to be an appropriate material to seal the root canals of overdenture abutments, because it has an inhibiting effect on demineralization at the cavity wall in vitro.

Aged↗

In vitro push-out strength of seven luting agents to dentin.

PURPOSE: New luting agents, described as resin-modified glass-ionomer cements and compomers, have been developed during the last decade to improve the retention of cemented restorations. The aims of this study were to (1) compare the push-out strength of these new luting materials against both conventional cements and bonding luting agents, and (2) evaluate the influence of dentin surface treatment on both glass-ionomer cement and 4-META adhesive resin push-out strength. MATERIALS AND METHODS: Conical standardized cavities were drilled in the center of coronal dentin disks. Ninety sandblasted Ni-Cr inlays, divided into nine batches, were luted into the cavities according to the surface treatment and the nature of the following luting agents: zinc phosphate cement, zinc polycarboxylate cement, type 1 glass-ionomer +/- polyacrylic acid, resin-modified glass-ionomer, polyacid-modified composite resin, filled bis-GMA phosphate ester resin, and 4-META adhesive resin +/- application of activated monomer. Each specimen was placed in a holding device, and a steel rod was used to apply a force on the inlay until rupture occurred. The push-out strength was calculated, and the failure mode was controlled. RESULTS: There were significant differences between some of the groups. The highest push-out strength was achieved by the 4-META adhesive resin after application of activated monomer. The lowest value was attained with zinc phosphate and polycarboxylate cements. CONCLUSION: Both resin-modified glass-ionomer and polyacid-modified composite resin luting materials exhibited a push-out strength similar to resin-based materials. Specific dentin surface treatments significantly enhanced the push-out strengths of glass-ionomer cement and 4-META adhesive resin.

Acid Etching, Dental↗

Microleakage of Class II packable resin composites lined with flowables: an in vitro study.

Flowable resin materials have been suggested as liners beneath packable composites to improve marginal integrity. This investigation evaluated the effect of low-viscosity liners on microleakage in Class II packable composite restorations. Twenty Class II cavities were prepared in extracted third molars for each of four packable composites (Heliomolar HB, Prodigy Condensable, Surefil and Tetric Condense). Ten restorations were placed for each material with their corresponding bonding agent per manufacturer's suggestion; in addition, 10 were placed with the flowable liner recommended by the manufacturer for that material. Samples were finished, stored in distilled water for at least 24 hours and thermocycled for 1,000 cycles between 5 degrees and 55 degrees C with a one-minute dwell time. Apices were sealed with epoxy cement and the teeth were varnished to within 1 mm of the margins. Samples were placed in 0.5% basic fuschin dye for 24 hours, rinsed, embedded in resin and sectioned to produce multiple sections. Microleakage was rated (0-4 ordinal scale) at both the occlusal and cervical margins. Data were analyzed with Kruskal-Wallis ANOVA for main effect and ranked sum analysis for pairwise testing (alpha = 0.05). All materials, either separately or in combination with a flowable liner, had greater leakage scores at the cervical margin compared to the occlusal margin. All packable systems tested did not yield a reduction in microleakage with the use of a flowable liner in vitro; however, the packable system with the flowable compomer used as a liner yielded significantly less overall microleakage compared to the three systems that used a resin composite liner.

Acrylic Resins↗

Effects of in-office bleaching products on surface finish of tooth-colored restorations.

A number of "high power" in-office bleaching products have recently been re-introduced into the market. The use of such strong oxidizing agents has raised questions as to possible adverse effects on tooth structure and restorative materials. This study evaluated the effects of 35% carbamide peroxide (Opalescence Quick) and 35% hydrogen peroxide (Opalescence Xtra) on the surface finish of four tooth-colored restorative materials (Spectrum TPH, Dyract AP, Reactmer and Fuji II LC). Twenty-seven matrix-finished specimens of each material were fabricated, stored in distilled water at 37 degrees C for seven days and randomly divided into three groups. Specimens in Group 1 were stored in distilled water at 37 degrees C (control). Specimens in Groups 2 and 3 were treated with 35% carbamide peroxide and 35% hydrogen peroxide, respectively. A total of three 30-minute bleaching sessions were conducted at one-week intervals. Storage medium during the hiatus period was distilled water at 37 degrees C. Surface roughness measurements were carried out using profilometry after each bleaching session. Data was analyzed using ANOVA/Scheffe's test at a 0.05 significance level. No significant difference in surface roughness was observed between the bleached and the control groups for all materials. In-office bleaching products are not detrimental to the surface finish of composites, compomers, giomers and resin-modified glass ionomer cements.

Analysis of Variance↗

The effect of different drying methods for single step adhesive systems on microleakage of tooth colored restorations.

The purpose of this in vitro study was to evaluate microleakage of tooth colored restoratives and accompanying single step adhesive systems using two drying methods (syringe air versus "sponge" applicator blotting). Eighty teeth were randomly assigned to four material groups. Class V cavity preparations, located half in enamel and half in cementum at the cementoenamel junction (CEJ), with a 1.0 mm enamel bevel were completed. The adhesive/composite groups included: (1) Single-Bond/Z-100 Composite, (2) Prime & Bond 2.1/Dyract AP Compomer, (3) OptiBond Solo Plus/Prodigy Composite, and (4) Scotchbond MultiPurpose/Z-100 Composite. Each material group (n=20), consisted of preparation Subgroups dried with syringe air (A), (n=10) and sponge applicators (B), (n=10). The preparations were conditioned, rinsed, and gently dried followed by placement of the primer/adhesive and restorative materials. All teeth were thermocycled, stained with methylene blue dye, invested in clear acrylic resin, and sectioned longitudinally through the center of the restoration. Readings were taken at the occlusal and gingival surface positions of each restoration section. A ratio (%) of wall length to amount of leakage along each wall was established. One-way Analysis of Variance (ANOVA) testing revealed: (1) no significant (p<0.05) differences existed between materials at the occlusal surface position in Subgroups A and B (syringe vs. applicator drying), (2) significantly (P<0.05) greater leakage of OptiBond Solo Plus compared to Single-Bond, Prime & Bond 2.1, and Scotchbond MultiPurpose at the gingival surface position in Subgroups A and B, (3) significantly (p<0.05) greater leakage of OptiBond Solo Plus compared to Single-Bond and Scotchbond MultiPurpose, combining the occlusal/gingival surface position scores, (4) no significant difference existed between Single-Bond Subgroups A/B, OptiBond Solo Plus Subgroups A/B, Scotchbond MultiPurpose Subgroups A/B, (5) significantly (p<0.05) greater leakage of Prime & Bond 2.1 Subgroup B compared to Subgroup A, (6) no significant (p<0.05) difference existed between material groups, except OptiBond Solo Plus (occlusal vs. gingival surface position), (7) no significant (p<0.05) difference between Single-Bond, OptiBond Solo Plus, and Scotchbond MultiPurpose comparing both Subgroups, same materials, and surface positions, and (8) no significant (p<0.05) difference existed between Prime & Bond 2.1, comparing both Subgroups, occlusal surface position. In the present study, significantly greater leakage was revealed with OptiBond Solo Plus compared to the other material groups, especially at the gingival surface positions. Significantly greater leakage was also recorded with OptiBond Solo Plus and Prime & Bond 2.1 sponge applicator drying as compared to the same materials, syringe air drying.

Absorption↗

Shear strength of sealants placed with non-rinse conditioning compared to a conventional acid etch-rinse technique.

The present study compared the shearing strength of a non-rinse conditioning (NRC) compomer sealant to that of a conventional sealant. Thirty molars and premolars were sectioned longitudinally. The resulting halves were divided into 4 groups. Cylinders of sealants were bonded to the sections using a gelatin capsule as a matrix, as follows: Group A (n = 15) NRC + Prime & Bond + Dyract Seal; Group B (n = 15) same as group A but with phosphoric acid and rinse; Group C (n = 15) conventional phosphoric acid + Helioseal; Group D (n = 15) NRC + Helioseal. After immersing the specimens in water for 7 days, the samples were mounted on the Instron testing machine at a 20 mm/min crosshead speed. The results were subjected to ANOVA with Scheffe test, with a significant level of .05. No shearing strength values could be obtained for the specimens of group D. Some cylinders separated from the enamel while still immersed in the water, and in others the adhesion was too low, and no readings could be observed. For all groups, failure occurred at the sealant-enamel interface. The mean value for group A was 5.33 MPa, significantly less than that of groups B 9.12 MPa and C 9.63 MPa (p < 0.006). No significant difference of the mean shear strength was evident between groups B and C. No rinse conditioning with Dyract Seal led to a considerably lower bond strength value than Dyract Seal and Helioseal with phosphoric acid and rinsing.

Acid Etching, Dental↗

Surface antibacterial properties of fissure sealants.

PURPOSE: Sealants form a physical barrier between the oral environment and deep fissures that contribute to caries prevention. It is postulated that sealants possessing antibacterial properties are advantageous. The purpose of this study was to evaluate the antibacterial properties of four pit and fissure sealants using direct contact test (DCT) and agar diffusion test (ADT). METHODS: For the DCT, 8 samples of Helioseal F (Vivadent), Ultraseal XT (Ultadent-Weldent), Conseal F (SDI), and Dyract Seal (Dentsply) were placed on the sidewalls of wells of a 96-microtiter plate. After polymerization, freshly grown Streptococcus mutans cells (circa 1 x 10(6)) were placed on the surface of each sample for 1 hour at 37 degrees C. Fresh media was then added to each well and bacterial growth was followed for 16 hours by temperature-controlled spectrophotometer. Similarly prepared samples were aged in phosphate buffered saline for 14 and 30 days and the DCT was repeated. The ADT was performed by placing samples in uniform wells punched in agar plates. RESULTS: Freshly polymerized samples in the DCT, Dyract Seal and Ultraseal XT possessed prominent antibacterial properties. Dyract Seal also demonstrated the most potent antibacterial properties, which lasted 14 days but faded within 30 days. In ADT, the halo in the bacterial lawn was measured after 48 hours, and only Dyract Seal demonstrated an inhibition zone. CONCLUSIONS: The compomer-based sealant Dyract Seal possessed the most potent and longest lasting antibacterial activity.

Analysis of Variance↗

Marginal adaptation of self-etching adhesives in Class II cavities.

PURPOSE: An in vitro dye-penetration study was performed on 2 different self-etching adhesives (Prompt L-Pop II, 3M-Espe; NRC, Prime&Bond NT Quix, Dentsply/DeTrey) in comparison to conventional conditioning of the cavity with phosphoric acid (Conditioner 36, P&B NT Quix, Dentsply/DeTrey). MATERIALS AND METHODS: Standardized mod cavities (one cervical margin in cementum, one in enamel) were prepared in 80 extracted human teeth (n = 20/group). Prompt L-Pop II was employed in two different sequences: A: placing the matrix band first, then applying the adhesive, B: first applying the adhesive, then placing the matrix band. The adhesive was cured in both cases. Conditioning and application of P&B NT Quix was carried out after placement of the matrix band in both procedures. The compomer Dyract AP was used as a restorative material in all cavities. After thermocycling, the median percentages of dye-penetrated (2% methylene blue) margins were determined separately for the cementum and enamel margins of the approximal boxes and for the lateral enamel margins of the boxes. RESULTS: The results showed that when using Prompt L-Pop II in Class II cavities, the adhesive should be applied before placing the matrix band (p < 0.0001). When the matrix band was placed first, P&B NT Quix showed superior results at a statistically significant level (p < 0.0001) in comparison to Prompt L-Pop II. There was no significant difference in the use of P&B NT Quix in combination with NRC or phosphoric acid in these Class II cavities. CONCLUSION: It is strongly recommended that Prompt L-Pop be applied prior to placement of the matrix band. Prime & Bond NT Quix seems to provide clinically relevant marginal seal in Class II cavities, regardless of whether NRC or phosphoric acid is used for conditioning.

Acid Etching, Dental↗