Therapy and prophylaxis of Tyzzer's disease. (In vivo antibiogram of three strains of B. piliformis).
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The delay between in-coming requests and results of bacteriological examinations was measured in a hospital laboratory before and after automation with an Abbott MS-2 system. Paradoxically, using this apparatus, which performs certain tasks within 6 hours instead of 12, increased the delay, mainly because of the multiple problems associated with automation. On the other hand, the delay was significantly reduced when a rapid micro-organism identification method (Micro ID) was used concomitantly. It is concluded that maximum efficiency could be achieved if bacteriological laboratories combined automated antibiotic susceptibility testing with a rapid germ identification method.
During the April 1979--June 1978 period, 1161 tests were carried out on the sensitivity to antibiotics and chemotherapeutical agents of Gram-negative bacillus strains isolated from 7632 urocultures. Resistance was tested under qualitative control against 13 antimicrobial agents by the Kirby-Bauer diffusimetric method. The resistance sensitivity and the presence of intermediary strains is shown for E. coli Citrobacter, Enterobacter, Klebsiella, Proteus, Morganella, Providencia, and Pseudomonas. Attention is drawn to the fairly high proportion of intermediary strains. Their role from the therapeutical point of view is reduced, but epidemiologically, their role is important since their evolution towards resistance or sensitivity cannot be foreseen.
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A total of 455 streptococcal cultures from bovine intramammary infection in 72 herds were studied to determine their sensitivity to 17 antimicrobial agents. More than 90% of the cultures were sensitive to carbenicillin, chloramphenicol, erythromycin, and penicillin. Gentamicin, neomycin, nitrofurazone, polymyxin, and streptomycin were ineffective in the in vitro inhibition of streptococci.
A total of one hundred and two cases of otitis media were screened for the isolation of bacterial flora of ears. Out of this, Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae accounting for 41.2%, 25.5% and 13.3% respectively were isolated. A further two hundred and four sputum samples from cases of lower respiratory tract infections were screened. Moraxella catarrhalis, S. aureus, S. pneumoniae and H. influenzae constituting 20.9%, 37%, 30% and 21% were isolated in that order. Thirty-one point seven percent of the Moraxella catarrhalis isolates were beta-lactamase positive. Beta-lactamase M. catarrhalis were resistant to penicillin and ampicillin while the non-beta-lactamase producers were sensitive to these antimicrobial agents. However, both beta-lactamase producers and non-producers were resistant to trimethoprim but sensitive to erythromycin, tetracycline and amoxycillin. Staphylococcus aureus, S. pneumoniae and H. influenzae was also sensitive to penicillin while S. pneumoniae was also sensitive to erythromycin and H. influenzae to chloramphenicol.
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