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At least 397 records · Page 22Linked to original sources
PubMed1990

Corynebacteria: incidence among samples submitted to a clinical laboratory for culture.

Over the period of one year, 83 corynebacteria isolates were identified in our laboratory, and their clinical relevance assessed by reference to patients whose clinical notes were available. Eleven species of corynebacteria were identified including four biotypes of C. jeikeium; six organisms were non-typeable; C. jeikeium and C. xerosis predominated. Species identified in the literature as causing clinical infection were also isolated--though in smaller proportions--as were strains of C. jeikeium which were not multi-resistant to antibiotics. Immuno-compromised patients and those with renal impairment had an increased frequency of corynebacteria. The isolation of C. jeikeium from the blood of a neonate suggests that this may be a potential pathogen in these patients. Antibiotic susceptibility of an organism was not a reliable marker of significance, and a reliable biotyping scheme should be adopted.

Bacterial Typing Techniques↗
PubMed1990

[Transcutaneous pulmonary fine-needle aspiration biopsy under fluoroscopic guidance. Our experience].

The authors report the radiological method and the cytohistologic and bacteriologic technique of percutaneous fine-needle aspiration biopsy of the lung under fluoroscopic guidance. The results are reported of 620 biopsies of benign and malignant lung lesions; the biopsies were performed from January 1981 to September 1989. Surgery, follow-up, and autopsy confirmed the high diagnostic accuracy of this method. The advantages of fluoroscopic guidance are discussed and compared with those of CT guidance.

Adolescent↗
PubMed1990

[Diagnostic errors in silicotuberculosis (clinical-autopsy comparisons)].

Basing on 42 post-mortem examinations of silicotuberculosis cases, the contributors display the most frequently encountered errors in diagnosing this disease. Silicotuberculous bronchadenitis, conglomerate-cirrhotic lower-lobe silicotuberculosis and their complications (e.g. concomitant pericarditis and endobronchitis) are the most difficult cases for detection. To avoid diagnostic errors, a most detailed case history study is needed, along with a complex clinico-functional and tomography examinations, a wide use of endoscopy and bacteriological techniques.

Autopsy↗
PubMed1979

The bacteriology of perimandibular space infections.

The bacteriology of 21 perimandibular clised-space infections was studied prospectively using optimal bacteriological techniques to collect, transport, and process specimens. There was an average of six microbial species per specimen, including approximately four anaerobes and two aerobes. The predominant aerobes were alpha-hemolytic and non-hemolytic streptococci; the predominant anaerobes were peptostreptococci, Bacteroides melaninogenicus, and Fusobacterium nucleatum. These findings indicate that most perimandibular space infections involve a polymicrobial flora in which anaerobic bacteria are the dominant isolates. Antimicrobial decisions should account for these observations, particularly in cases where cultural data are not available or when anaerobic cultures are not performed with optimal techniques.

Adult↗
PubMed1987

[Nonclostridial obligate anaerobes--the main causative agents of lung abscesses].

The use of the anaerobic technique in bacteriological investigations has made it possible to establish the leading role of asporogenous anaerobes in the etiological structure of the causative agents of pulmonary abscesses. Asporogenous anaerobes have been isolated from 93% of all examined patients, and in 54.4% of such patients these microorganisms have proved to be an independent etiological factor. Among asporogenous anaerobes, the main causative agents of pulmonary abscesses have been found to belong to four genera: Bacteroides, Fusobacterium, Peptococcus and Peptostreptococcus. Pulmonary abscesses of purely aerobic etiology are rather rare (5.3%). The schemes of the antibacterial treatment of patients with pulmonary abscesses, developed with regard to the leading etiological role of asporogenous anaerobes and clinically approved, have permitted achieving a considerable improvement in the results of treatment and an essential economic effect.

Anti-Bacterial Agents↗
PubMed1987

[Submerged-phase gel electrophoresis in the analysis of plasmids for epidemiologic purposes].

The validity of the submarine agarose gel electrophoresis as routine method for plasmidic epidemiology was considered. Using standard plasmids, the efficiency of a performed protocol conditions (5 V/cm for 3 hours, 0.8% agarose) in screening plasmids of different size was studied. The results provide sharp profile resolution and an accurate estimate of molecular weights in the range from 1.2 to 112 megadaltons.

Bacterial Typing Techniques↗
PubMed1977

[Current problems of medical microbiology: Achievements, tasks and perspectives].

Two important problems of current medical microbiology are discussed in this paper: 1) a change of etiological structure of infectious morbidity-elevation of the incidence of infections caused by conditioned pathogenic causative agents, and 2) postvaccinal complications. In the light of these problems the tasks of microbiological investigations for the immediate future are discussed. Tasks and prospects of study of the molecular structure of the pathogenicity factors of importance for the creation of chemical vaccines and genetic investigations which would provide construction of strains and preparations useful in practice are formulated.

Bacteriological Techniques↗
PubMed1986

Prevalence of pathogenic bacteria in patients with sore throat.

From 361 females and 452 males (527 primary care out-patients and 286 in-patients) a total of 813 throat swabs were examined for bacterial pathogens by conventional bacteriological techniques. Beta-haemolytic streptococci were grouped by the coagglutination method. Throat swabs from 14% of males and 15.6% of females were positive for bacterial pathogens; 75 (64% specimens grew BHS group A. All age groups of both sexes yielded a similar proportion of organisms. All pathogens were sensitive to penicillin, erythromycin and cephradine.

Adolescent↗
PubMed1987

Characterization and identification of Mycobacterium smegmatis in bovine mastitis.

Twenty morphologically similar strains of a Mycobacterium sp isolated from milk of cows with bovine mastitis in the US Department of Agriculture dairy herd were identified as M smegmatis. Mastitis isolates submitted from Vermont and Washington also were identified as M smegmatis. A scheme for presumptive identification of rapidly growing mycobacteria was developed, using culture media and bacteriologic techniques commonly used in mastitis diagnostic laboratories. Important tests used were acid-fast staining, rapidity of growth, pigmentation, nitrate reduction, arylsulfatase activity, mucic acid utilization, acid production from galactose, rhamnose, and inositol, and growth at 45 C and 52 C.

Animals↗
PubMed1985

[The bacteriology laboratory of tomorrow].

The development of needs and the conditions for performing examinations in clinical bacteriology leads us to consider that bacteriological analysis should eventually: be closer to clinical requirements. This results in an accelerated bacteriology (whose response-times approach those of other biological specialties) and in a consideration of the diagnostic and therapeutic importance of the response; be less costly. This leads to an increase in productivity, and involves an introduction of computers, automatic systems for antibiograms and identification, and appropriate and inexpensive bioreagents; consider the requirements of the technicians in order to avoid diminishing the interest of the work. This development demands a recognition of needs, whether expressed or not, and a rejection of olds habits that are not adapted to the requirements of a bacteriology which is closer to the patient, quicker, and finally, less costly.

Bacterial Infections↗
PubMed1979

Yersinia pseudotuberculosis infection in a cat.

A diagnosis of Yersinia pseudotuberculosis in a cat was established by microbiological and histological means. Clinical signs were general malaise, anorexia and vomition. Significant laboratory findings included a serum glutamicpyruvic transaminase elevation and the presence of urine bilirubin and urobilinogen. Histological examination of liver biopsies revealed numerous varying-sized foci of caseation necrosis containing large numbers of Gram-negative coccobacilli. Bacteriological techniques, treatment and control measures are discussed.

Animals↗
PubMed1986

[Interrelation of the enterotoxigenic properties and the antigenic structure of Escherichia].

The enzymatic signs and serological characteristics of Escherichia enterotoxigenic strains isolated from patients with acute intestinal diseases and from healthy persons were studied. The cultures were subdivided into 24 enzymatic variants and classified with 48 serogroups and 61 serovars. The enterotoxigenic properties of the strains were compared with their serological characteristics and enzymatic signs. The strains, isolated from different persons and classified with the same serovar, belonged to the same variant with respect to the type of enterotoxin they produced (only thermostable enterotoxin, only thermolabile enterotoxin, or both), were similar in the degree of their toxigenicity and belonged, as a rule, to the same enzymatic variant. The data on the presence of manifest interrelation between the enteropathogenicity of Escherichia and their structure, as well as on the stability of the enterotoxigenic properties of these organisms, indicate that in acute intestinal diseases the determination of Escherichia enterotoxigenic strains can be carried out by common bacteriological techniques with the use of specific agglutinating sera.

Acute Disease↗