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At least 397 records · Page 22Linked to original sources
PubMed2005

Contamination of Salmonella in retail meats and shrimps in the Mekong Delta, Vietnam.

From March 2000 to September 2001, 608 samples of retail meat (136 pork, 70 beef, 202 chicken, and 200 ducks) and 110 samples of retail shrimp from six provinces of the Mekong Delta in Vietnam were collected individually and examined for the prevalence of Salmonella. Of the 718 samples examined, 243 (33.8%) were Salmonella positive. Salmonella was isolated from 69.9% of the pork samples, 48.6% of the beef samples, 21.0% of the chicken meat samples, 22.3% of the duck meat samples, and 24.5% of the shrimp samples. From 261 Salmonella isolates, 24 different serovars were identified. The predominant serovars of the isolates were Salmonella Derby, Salmonella Weltevreden, and Salmonella London in pork; Salmonella Weltevreden, Salmonella London, and Salmonella Dessau in beef; Salmonella Emek, Salmonella Typhimurium, and Salmonella Dessau in chicken meat; Salmonella Lexington, Salmonella Derby, and Salmonella Dessau in duck meat; and Salmonella Weltevreden, Salmonella Tennessee, and Salmonella Dessau in shrimps. Salmonella Bovismorbificans, Salmonella Derby, Salmonella Dessau, and Salmonella Weltevreden were the most common serovars in all the samples examined. These results indicate a high rate of contamination by Salmonella in retail meats and shrimps in the Mekong Delta, Vietnam.

Animals↗
PubMed1976

[Diet influence on growth-characteristics of the shrimp Crangon Crangon L., estimated by the evolution of nucleic acid content (author's transl)].

1. Young sand-shrimps having an initial weight of 63 mg were reared for 4 months with a natural diet (A) and a compounded diet (B). 2. Growth is studied, both by the evolution of the fresh weight and the variation of cell-number and cell-size; these are estimated from total DNA and fresh-weight/DNA ratio evolution. 3. With diet A, the mean weight increment is a linear function of time, the daily weight-gain being equal to 2.8 mg/day. The increase in total DNA content per shrimp is a linear function of fresh weight; its value goes up from 187 to 1020 micromoles. The fresh-weight/DNA ratio, initially equal to 337.9 (mg/micromoles) ,remains constant. For the whole experiment, growth is only a result of cellular multiplication. 4. With diet B, the weight gain is inferior to that observed with diet A. The fresh weight gain curves show two visibly homogenous steps, each with a distinct growth-rate; the mean daily weight gain is equal to 1.7 mg/day during the first two months, and equal to 2.6 mg/day for the two following months (fig. 1). In reality, from the total DNA and fresh-weight/DNA ratio evolution, 3 successive steps can be discerned (fig. 2, fig. 3). During the first month, total DNA increases from 187 to 418 micromoles and fresh-weight/DNA ratio falls down to 273.9, a-19% decrease. For this period, growth is only the result of cellular multiplication, and the decrease of the weight-gain in comparison with diet A is attributed to a decrease in cellular size. During the second month, as the fresh weight increases from 114.5 to 161.2 mg, total DNA increases from 418 to 519 micromoles and the fresh-weight/DNA ratio returns to its initial value. Two thirds of the growth is the result of cellular multiplication, and the rest being due to cell-size enlarging. For the last two months, as the mean fresh weight increases from 161.2 to 296.7 mg, the DNA per shrimp, hence the cell-number, remains constant; but the fresh weight/DNA ratio, or the cell-size, is multiplied by 1.93. 5. By the method used for this study, it is possible to evidence influence of diet on the growth mechanisms themselves (cellular multiplication and cell enlarging). 6. The influence of diet on the growth of the shrimp could be explained by, either a diet qualitative deficiency, or a food-intake decrease. The possibility of a hormonal control has been suggested.

Aging↗
PubMedDate not supplied

Isolation of Vibrio and Pseudomonas from brown shrimp (Penaeus californiensis Holmes) intestine.

Bacteria of the genera Vibrio, Pseudomonas and Aeromonas were isolated from the intestine of apparently healthy brown shrimp (Penaeus californiensis Holmes) cultured in a tidal pond. Species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. Isolation was done first in Marine broth, then in selective media (TCBS, Cetrimide and MacConkey). The oxidase negative strains were discarded as insignificant to shrimp culture. The identification of oxidase positive strains was based in morphological and colonial characteristics, biochemical capabilities, and both salinity and temperature tolerance. API 20E system and fatty acid analysis were also included. Three potentially pathogenic bacteria, Vibrio parahaemolyticus, Vibrio furnissii and Pseudomonas putida were isolated and identified from healthy shrimp intestine.

Animals↗
PubMed2001

Effects of nutritional factors and soil addition on growth, longevity and fecundity of the tadpole shrimp Triops newberryi (Notostraca: Triopsidae), a potential biological control agent of immature mosquitoes.

The notostracan tadpole shrimp (TPS) Triops newberryi Packard has potential to be used as a biocontrol agent of immature mosquitoes. Eggs, nymphal or adult shrimps are considered to be the stages for field introduction. To yield good growth of the shrimp and high production of shrimp eggs under artificial conditions, nutritional requirements of TPS for growth, survival and fecundity need to be elucidated. In the laboratory, we evaluated various nutritional and edaphic regimens, such as soil alone, mosquito larvae or rabbit pellets alone and various combinations of these three components for culturing. These factors influenced the growth, longevity and egg production profoundly. It was shown that the simulated natural conditions, i.e. full combination of all three factors, yielded the largest TPS with longest survival and highest egg production, followed by the combinations of any two components. Any single component, soil, mosquito larvae, or rabbit pellets, did not result in good growth, survival and egg production. By formulating optimal rearing substrates, this species of TPS will yield large numbers of all stages for experimentation and field introductions. Under optimal conditions, they mature in 7-8 days and survive for about one month. Each TPS is capable of producing up to 1,000 eggs during its lifetime. These studies developed nutritional regimens for TPS mass culturing procedures, where the eggs, nymphal and adult TPS can be mass cultured for field introduction and stocking in mosquito developmental sites.

Animal Feed↗
PubMed2001

Effect of probiotics on bacterial population and health status of shrimp in culture pond ecosystem.

The artificially manufactured probiotics having beneficial bacteria, Bacillus spp. was applied regularly in a modified extensive shrimp (Penaeus monodon) culture pond, located on the bank of Vellar estuary, Parangipettai. The populations of total heterotrophic bacteria (THB), beneficial bacteria (Bacillus spp.) and pathogenic bacteria (vibrios) were monitored in water and sediment of the pond. The results were compared with a control pond, situated in the same location having same water spread area, stocking density, species managed with same technologies and optimum environmental parameters in which no probiotic was applied. The populations of THB and Bacillus spp. in the experimental pond increased and the vibrios decreased after each application of probiotics. But the result of the control pond showed an increasing trend of the populations of THB, Bacillus spp. and vibrios towards days of culture. The control pond had lower levels of THB and Bacillus spp. and higher levels of vibrios than the probiotic applied (experimental) pond. Also the probiotics maintained optimum transparency and low organic load in the experimental pond as compared to control. In general, water and sediment had almost equal number of Bacillus spp. and vibrios, but sediment had higher THB load than water. The applications of probiotics lesser pathogenic vibrios and enhance beneficial bacilli in the culture leading to improved water quality, promoted growth and survival rates and increased the health status of the shrimp without stress and disease outbreaks. Thus the application of probiotics could lead to disease-free and profitable shrimp culture operations which will be helpful for shrimp farmers as most of them are now-a-days severely affected by microbial diseases.

Animals↗
PubMed2002

[Preliminary study on anti-baculovirus mechanism of feeding housefly larvae (Musca domestia) and population infection model of outbreaking epidemic disease of shrimp (Panaeus chinensis)].

Based on indoors individual infection and outdoor population infection of shrimp, population infection model was established and anti-baculovirus mechanism of housefly larvae was analyzed. The results showed that 50% death time of the shrimps feeding with housefly larval reasonably was prolonged 3-5 times compared with the control group. Prophenoloxidase system of prawns was activated while feeding shrimps with housefly larval 7-10 days before infection by baculovirus, and prophenoloxidase activity was 3 times higher than the control group. The establishment of population infection model illustrated clearly important role of housefly larvae in improving the immunity of shrimps from baculovirus.

Animals↗
PubMed2003

Physico-chemical characteristics of the Vellar estuary in relation to shrimp farming.

All the physico-chemical parameters such as temperature, LEC, salinity, pH, dissolved oxygen and nutrients like total phosphorus, inorganic phosphate, nitrite and silicate studied in relation to shrimp farming. There are as many as 42 shrimp farms situated on the banks of Vellar estuary. These farms discharge the used water into the estuary, which may influence the biota there. In the present study the physico-chemical feature in relation to shrimp farming were studied in 3 stations of the estuary. When compared with the previous data from Vellar estuary there was no much difference in physico-chemical characteristics due to shrimp farming.

Animals↗
PubMed2004

[Effects of different temperatures on the growth and energy budget of Chinese shrimp, Fenneropenaeus chinensis].

The effect of temperature on the growth and energy budget of Chinese shrimp Fenneropenaeus chinensis was studied at 18, 22, 25, 28, 31 and 34 degrees C. The results showed that its specific growth rate in terms of body weight and energy accumulation increased with temperature between 18 degrees C and 31 degrees C, and then decreased significantly at 34 degrees C. The similar trend was observed in food consumption and apparent digestion rate. The food conversion efficiency in weight and energy were 28.99%-53.09% and 15.70%-7.24%, respectively, which decreased with increasing temperature. The optimum temperature for the growth of shrimp was calculated from the relationship of SGR, which was 29.7 degrees C in this study. The energy budget of shrimp showed that the energy assimilated from food decreased with increasing temperature, while that spent in metabolism increased with increasing temperature. The results indicated that the high growth rate of Chinese shrimp at suitable temperature mainly resulted from the significant increase of food consumption and apparent digestion rate at corresponding temperature regimes.

Algorithms↗
PubMedDate not supplied

Multiresidue determination of fluoroquinolones in shrimp by liquid chromatography-fluorescence-mass spectrometry.

An efficient multiresidue method for analysis of fluoroquinolones in shrimp has been developed in which quantitation by fluorescence and confirmation by Multiple Stage Mass Spectrometry (MS) is achieved simultaneously. In this method, shrimp tissue is extracted with ammoniacal acetonitrile and the extract is defatted and then evaporated. After dissolution in basic phosphate buffer, fluoroquinolones in the extract are separated by liquid chromatography and quantitated, taking advantage of their intense fluorescence. Eluate from the fluorescence detector enters the MS, which allows for confirmation by monitoring ratios of 2 prominent product ions in the MS3 or MS2 spectrum. Using this method, 8 fluoroquinolones have been analyzed in shrimp samples fortified at 10, 25, 50, or 100 ppb levels. Recoveries for desethyleneciprofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin, and difloxacin ranged from 75 to 92%, with relative standard deviation values of <6%. The limits of quantitation ranged from 0.1 to 1 ng/g. Enrofloxacin and ciprofloxacin were also successfully determined in enrofloxacin-incurred shrimp using this method.

Acetonitriles↗
PubMed2005

[Analysis of chloramphenicol in shrimps by gas chromatography tandem mass spectrometry].

OBJECTIVE: A sensitive method based on isotope dilution gas chromatography tandem mass spectrometry is described for the determination of the antibiotic chloramphenicol (Cap) in shrimp tissue. METHODS: Homogenized shrimp samples spiked with an isotopically labeled internal standard (d5-Cap) were extracted with ethyl acetate extraction, defatted with hexane, followed by a clean-up step on a SPE C18 cartridge, and then the elute was evaporated and derivatized with Sylon BFT {N,O-bis (trimethylsily) trifluoroacetamide[BSTFA]-trimethylchlorosilane [TMCS] ,99 + 1}. Analyzes were determined by GC/MS-MS in MRM mode. RESULTS: Internal calibration curves obtained for Cap/ d5-Cap in the 5 to 500 pg range were linear with correlation coefficients of 0.9958. Limit of quantitation (LOQ) was 0.3ng/g. Shrimp tissues were fortified with Cap at 0.3, 0.5, 1.0 ng/g level, average recoveries were 104%, 111% and 95% , respectively. CONCLUSION: The proposed method allows the detection, quantitation and confirmation of Cap in shrimps with high accuracy and sensitivity.

Animals↗
PubMedDate not supplied

Liquid chromatographic determination of the processing aid 4-hexylresorcinol in shrimp.

A rapid, sensitive, liquid chromatographic (LC) method has been developed for determination of residuals of the processing aid, 4-hexylresorcinol, on shrimp meat. An aqueous homogenate of shrimp meat is extracted with ethyl acetate followed by precolumn preparation on a silica Sep-Pak cartridge. LC determination is preformed with a Nova-Pak C18 column, with UV detection at 214 nm. Sensitivity was 0.006 micrograms, and recovery from shrimp meat samples of known 4-hexylresorcinol addition was 94%. Shrimp treated with 4-hexylresorcinol under the recommended dip protocol had mean residuals of 1.18 ppm, with a standard deviation of 0.13 ppm.

Animals↗
PubMed1991

[Progebiophilus bruscai (Isopoda:Bopyridae) parasitizing the shrimp Upogebia dawsoni (Thalassinoidea:Upogebiidae), in Baja California Sur, Mexico)].

Parasitism of the isopod Progebiophilus bruscai Salazar-Vallejo & Leija-Tristán over the common mud shrimp, Upogebia dawsoni Williams, was analysed in the Ensenada and Bahia de La Paz, Baja California Sur, Mexico. Mud-shrimps were collected in three similar sites that differ in grain size and in the anthropogenic organic matter enrichment. Four-hundred-nine mud-shrimps were collected; the largest abundance was registered in the organically enriched site, but they were significantly smaller and more heavily parasited than the animals from the site lacking such organic enrichment. The size of the parasite is clearly dependent on the size of the mud-shrimp. This is the first study of the upogebiid-bopyris relationship in Mexico.

Animals↗
PubMed1990

A case of a salivary calculus containing a limb of a shrimp--the structural analysis.

This study describes a case of a salivary calculus which contained the limb of a shrimp. Pathological findings seemed to show a stratiform structure of calculus around the foreign body at the center. However, when the cut surface of the salivary calculus was examined by a scanning electron microscope, it was suspected that the origin of the calculus was in another area next to the foreign body. As a result, it became clear that the foreign body was not the core, but that the core of the salivary calculus was somewhere else, and that the earlier foreign body theory needed to be reconsidered. As to the foreign body, the patient remembered eating a shrimp, which was probably the foreign body in question. The findings obtained from the analysis of other shrimp limb specimens were structurally similar. Therefore the suspicion that the foreign body was indeed the limb of a shrimp was increased.

Adult↗
PubMed1978

Decomposition in foods (chemical methods). Fluorometric determination of indole in shrimp.

A fluorometric method is described for determining indole in shrimp. The indole is extracted with n-hexane, partitioned into a methanol-saturated sodium chloride solution (9+1), and determined fluorometrically. The detection limit of the method is 0.04 microgram indole/g shrimp. Quantitative analytical data are presented for indole in samples of shrimp determined by the fluorometric method and the AOAC official colorimetric and gas-liquid chromatographic procedures. The correlation coefficient between the data of the fluorometric and colorimetric methods was 0.96. Indole recovered from 25 g samples of fresh shrimp spiked with 6 and 12 microgram indole/25 g ranged from 97 to 106%. The fluorescence response is linear in the range of 1 to 25 microgram indole/100 ml methanol solution and no significant change is noted in solutions kept in the dark 18 hr.

Decapoda↗
PubMed1981

High pressure liquid chromatographic method for indole in shrimp: development of method and collaborative study.

A collaborative study on the determination of indole in shrimp was conducted in which a high pressure liquid chromatographic (HPLC) method and a spectrofluorometric method were compared with the AOAC gas-liquid chromatographic (GLC) method (18.075-18.078, 13th ed.). In the HPLC method, 10 g shrimp was blended with methanol, an internal standard was added, and the extract was filtered. Indole was separated on an octadecylsilane reverse phase column, using 60% MeOH-H2O, and quantitated with a fluorescence detector (excitation 280 nm, emission 330 nm) by comparing the indole peak height with that of an internal standard, 2-methyl-indole. Recoveries at a 25 micrograms/100 g level averaged 104% with a range of 90-127%, and at a level of 35 micrograms/100 g averaged 102% with a range of 93-112%. In the spectrofluorometric method, 25 g shrimp was extracted with 2% EtOAc-hexane. After several washes, indole was partitioned into a saturated NaCl-MeOH solution and its fluorescence was measured (excitation 280 nm, emission 332 nm). Recoveries at a 25 micrograms/100 g level averaged 93% with a range of 0-255% and at a level of 35 micrograms/100 g averaged 64% with a range of 0-107%. Recoveries obtained by the AOAC-GLC method at a level of 25 micrograms/100 g averaged 96% with a range of 81-116% and at a level of 35 micrograms/100 g averaged 101% with a range of 81-119%. The coefficients of variation were 20, 10, and 64% at a 25 micrograms/100 g level for the GLC method, the HPLC method, and the spectrofluorometric method, respectively. The HPLC method was adopted as official first action for indole levels in shrimp exceeding 1 microgram/100 g.

Animals↗
PubMed1994

Characterization of insulin receptor from the muscle of the shrimp Penaeus japonicus (Crustacea: Decapoda).

The beta-subunit of the insulin receptor from the muscle of the shrimp Penaeus japonicus exists as multiple subtypes with M(r) of 79,000, 77,000 and 75,000. Only the subunit of M(r) 79,000 is autophosphorylated after the addition of insulin. The autophosphorylation occurred specifically at Tyr residues, as demonstrated by the specific subsequent dephosphorylation by the phosphotyrosyl protein phosphatase from the human placenta. The detergent, Triton X-100, and the metal ion, Mn2+, caused a noticeable enhancement of the autophosphorylation of shrimp insulin receptors from the muscle. Okadaic acid activated the kinase activity of the insulin-stimulated insulin receptor, but not the basal activity of the insulin receptor without the addition of insulin. Further studies comparing the insulin binding of the shrimp insulin receptor in the regulation of kinase activity of the multiple beta-subunit subtypes from the shrimp muscle are under way.

Amino Acid Sequence↗
PubMed1993

[Hygienic and commercial quality of Senegalese frozen shrimp].

100 samples of shrimps (frozen entire raw shrimps: FERS and frozen peeled raw shrimps: FPRS) collected in one urban factory, were studied to assess their hygienic and commercial quality. In addition to bacteriological analysis, the dosage of residual bisulphite content was carried out. It appeared that: level of aerobic plate was high for FERS: 2.55.10(4)/g and weak for FPRS: 2.45.10(5)/g; level of psychrotophic micro organisms was relatively high for FERS: 1.07.10(4)/g and very weak for FPRS: 0.53.10(4)/g; level of fecal coliform was high for fers: 30.62/g and FPRS: 68.75/g; Staphylococcus aureus and Anaerobic sulphite reducing organisms counts were low; neither salmonella nor Vibrio parahaemolyticus was found; residual bisulphite content is higher than the standard in 10 p.100 of samples. The upholding of the exported level of senegalese shrimps implies and improvement of their hygienic quality.

Animals↗
PubMedDate not supplied

Improved method for determination of sulfites in shrimp.

A method for the determination of sulfites in shrimp developed by Kim and Kim and modified by Paíno-Campa et al. for sausages has been further modified. Total sulfites are extracted by initially adding aluminum oxide to the sample and using an extraction solution at pH 12. In the reproducibility assays, coefficients of variation (CVs) were 4.8% for free sulfite and 7.8% for total sulfite in the edible part of the shrimp. In the inedible part, CVs were 7.5% and 8.9% for free and total sulfite, respectively. Mean recovery was 87.8% for the edible part and 84.4% for the inedible part of the shrimp. The method was compared with the optimized Monier-Williams method. The results obtained with both methods for determination of total SO2 in the edible part of the shrimp did not show significant differences (P = 0.05).

Animals↗