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Results for “SHIKIMIC ACID”

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At least 397 records · Page 22Linked to original sources
PubMed1978

Purification and characterization of quinate (shikimate) dehydrogenase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa.

The bifunctional enzyme quinate (shikimate) dehydrogenase (quinate: NAD+ oxidoreductase, EC 1.1.1.24), which catalyzes the first reaction in the inducible quinic acid catabolic pathway of Neurospora crassa, has been purified to homogeneity. The enzyme is a monomer of 41000 daltons with an s20,w = 2.94 S. However, electrophoresis under non-denaturing conditions revealed three protein species, which have both quinate and shikimate dehydrogenase activities. The enzyme, with a single binding site for both substrates, has a Km of 0.37 mM for quinate and of 1.18 mM for shikimate, although the V is about 3-fold higher with shikimate. Essential sulphydryl groups which were not localized in the active site were detected. Thermal stability of the enzyme was greatly enhanced by low concentrations of quinate, shikimate, NADH, or by high ionic strength.

Alcohol Oxidoreductases↗
PubMed2006

High shikimate production from quinate with two enzymatic systems of acetic acid bacteria.

3-Dehydroshikimate was formed with a yield of 57-77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.

Acetobacteraceae↗
PubMed1975

[Growth and sexual reproduction of the dermatophyte Arthroderma vanbreuseghemii Takashio (Trichophyton mentagrophytes (Robin blanchard) on a synthetic medium].

A synthetic medium without agar is proposed for the growth and the sexual reprpduction of the dermatophyte Arthroderma vanbreuseghemii (Trichophyton mentagrophytes). The determinations of mycelium dry weight showed that this species grew poorly with only one amino-acid as nitrogen source. A. vanbreuseghemii grew weakly on a mixture of glutamic and aspartic acid and serin. A small amount of aromatic amino-acids added to this mixture, increased the growth rate comparable to that obtained on peptone. The sexual reproduction was induced on synthetic medium with a propicious balance between carbon and nitrogen sources. Addition of 0.5 or 1g/1 of glucose to 50 mg N/1 supplied by the amino-acids mixture (including the aromatics) gave the optimal ratio. Using these conditions, many cleistothecia was produced by crossing two semi-compatible strains.

Amino Acids↗
PubMed2003

Antioxidant activity of 3-dehydroshikimic acid in liposomes, emulsions, and bulk oil.

The antioxidant activity of 3-dehydroshikimic acid (DHS), an intermediate in the biosynthesis of aromatic amino acids, was evaluated in three assay systems: bulk oil (lard), liposomes, and a 10% corn oil-in-water emulsion. Upon initiation of peroxidation in the liposome or emulsion systems, DHS exhibited weak antioxidant activity. In contrast, DHS displayed strong antioxidant activity in lard, suppressing peroxidation with activity comparable to that of tert-butylhydroquinone, propyl gallate, and gallic acid and superior to that of alpha-tocopherol. Two major DHS oxidation products, gallic acid and protocatechuic acid, were identified by gas chromatography/mass spectral analysis of lard extracts; both compounds are effective antioxidants in the bulk oil system. In the liposome system, DHS remained intact throughout the assay period. A small amount of gallic acid was observed in extracts of the emulsion; however, protocatechuic acid was not detected. A mechanism to explain the different activities of DHS in the three lipid systems is proposed.

Antioxidants↗
PubMed2003

Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize.

Laser-capture microdissection (LCM) allows for the one-step procurement of large homogeneous populations of cells from tissue sections. In mammals, LCM has been used to conduct cDNA microarray and proteomics studies on specific cell types. However, LCM has not been applied to plant cells, most likely because plant cell walls make it difficult to separate target cells from surrounding cells and because ice crystals can form in the air spaces between cells when preparing frozen sections. By fixing tissues, using a cryoprotectant before freezing, and using an adhesive-coated slide system, it was possible to capture large numbers (>10,000) of epidermal cells and vascular tissues (vascular bundles and bundle sheath cells) from ethanol:acetic acid-fixed coleoptiles of maize. RNA extracted from these cells was amplified with T7 RNA polymerase and used to hybridize a microarray containing approximately 8800 maize cDNAs. Approximately 250 of these were expressed preferentially in epidermal cells or vascular tissues. These results demonstrate that the combination of LCM and microarrays makes it feasible to conduct high-resolution global gene expression analyses of plants. This approach has the potential to enhance our understanding of diverse plant cell type-specific biological processes.

Aquaporins↗