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At least 397 records · Page 22Linked to original sources
PubMed1997

[Microscopic identification of the powder of roots of genus Adenophora: II. The roots of Sect. Remotiflorae and Sect. Adenophora].

This paper deals with the microscopic characteristics of the powder of the roots of 11 species and subspecies of Genus Adenophora. The results show that Sect. Basiphyllae, Sect. Pachydiscus, Sect. Remotiflorae and Sect. Adenophora can be identified based on the amount and thickness of the wall as well as the characteristics of the pit of sclerified cork cells, and 29 species, subspecies and varieties can be identified based on the amount, shape and thickness of the walls of the sclerified cork cells, the diameter and pit of the vessels, the number and shape of the secretion of the laticifers, and the number of inulin crystals.

Magnoliopsida↗
PubMed2000

[Pharmacognosical study on secondary metabolites].

Clonal micropropagation on various medicinal plants was set up resulting in the regenerated plants which possessed a homogeneous quality. The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization of mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigene-BSA conjugate with mouse myeloma cells. Competitive enzyme-linked immunosorbent assay (ELISA) using MAb was set up as a high sensitive, specific and reproducible qualitative method. A method of determination for ginsenosides by using a unique western blotting was established. Immunoaffinity column chromatography using an anti-ginsenoside Rb1MAb has made possible a single-step separation of ginsenoside Rb1 from a crude ginseng extract. Single chain Fv gene of anti-forskolin MAb was prepared from mRNA of hybridoma secreting anti-forskolin MAb and cloned. Gene was constructed into a pET-28a(+) vector producing a scFv protein. Modeling of forskolin and scFV was investigated. THCA synthase was purified from the homogenate of Cannabis sativa leaves on successive column chromatographies. THCA synthase was confirmed to be homogeneity having 75 kDa. To obtain the corresponding cDNA clone of THCA synthase, a set of degenerate promers was constructed based on N-terinal and internal amino acid sequences of THCA synthase. The 5' and 3' ends of cDNA were amplified by RACE. A full sequencing has been determined to be corded a polypeptide having 545 amino acid residues. The cDNA clone was expressed in yeast system via PUC19 vector resulting in THCA synthase activity.

Animals↗
PubMed1997

[Commercial identification and resources survey on Chinese drug radix Ranunculi ternati].

Based on the collection of commercial products field investigation in distribution areas and main production places, and consultation of over 200 specimens the identification of traditional Chinese drug Maozhaocao was carried out. The results show that the present source of commercial "Maozhaocao" derives from 2 species of genus Ranunculus. The main species is Ranunculus ternatus, while R. polii is used only in a few regions. A resources survey of the drug was also conducted.

Conservation of Natural Resources↗
PubMed1997

[Continuity and changes of breeds of fupenzi].

Based on textual researches, it has been confirmed that Fupenzi first appeared in Han Dynasty. Both Fupenzi and Penglei had the same origin, but split up during Jin Dynasty into two different breeds. Through ages Fupenzi has been taken as the fruits of the plant Rubus of Rosaceae species. Among them, R. idaeus, R. corchorifolius and R. coreanus have long been in use. R. chingii is the new breed of modern Fupenzi.

Conservation of Natural Resources↗
PubMed1997

[Ontogeny of Curcuma longa L].

Based on the research of growth of organs, accumulation of dry matter, distribution of photosynthetic production and displacement of growth centre, the growth of turmeric can be divided into three stages. There are 13 leaves in the life of turmeric. Leaf formation takes place rapidly to form 8 leaves in the seedling stage. The leaf area and increase of dry matter per day in roots and leaves are smaller or lower in the seedling stage. In the daughter rhizome formation stage, NAR reaches the maximum (3.54/m2.d), and the leaf area reaches the maximum(3302.9 cm2/plant, LAI 4.95). Before the daughter rhizome formation stage, more than 75% of dry matters are distributed in leaves, and in the daughter rhizome formation stage, 50%-75% of dry matters are distrbuted in leaves. In the late growing period, more than 40% of dry matters are distributed in the daughter rhizome. The growth centre of turmeric changes two times in life. When CGRr is equal to CGRt, the crossing of CGRt and CGRr curves may be regarded as the evidence of the transfer of growth centre from leaves to daughter rhizome.

Pharmacognosy↗
PubMed1997

[Identification of Scutellaria barbata D. Don and its adultrants].

Studies have been made on the comparison and identification of morphological and anatomical characteristics of six species Scutellaria barbata and its adultrants. Several tables and drawings are given. This paper may be helpful to pharmacognosists for identifying commercial crude drugs.

Drug Contamination↗
PubMed1997

[Determination of oleanolic acid contents in differently-processed Achyranthes bidentata Bl. by HPLC].

The contents of oleanolic acid in differently-processed products of Achyranthes bidentata were determined by HPLC. The result showed the contents to be in the following sequence: salt-broiled product > alcohol steamed product > alcohol-broiled product > unprocessed product. The average recovery rate was 100.2% and RSD 1.54%. The determination method has thus proved up to the standard for quantitative analysis.

Chromatography, High Pressure Liquid↗
PubMed2001

[Pharmacognostical studies on cortex Indigoferae Pseudotinctoriae].

This paper reported the studies on descriptions, microscopical characteristics of cross section and podwer, chemical examination and TLC of Cortex indigoferae pseudotinctoriae. These studies provided referential information for clinics, quality control, development and identification of this crude drug.

Drugs, Chinese Herbal↗