[Bacteriological peptone obtained from residues of human placenta (author's transl)].
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Study of some parameters of the process of hydrolysis in addition to other experiments has made it possible to develop a method for obtaining a nutrient base for the cultivation of microorganisms with the use of cattle whole blood as protein substrate and papain as a hydrolyzing agent. The product thus obtained is characterized by high levels of amino and total nitrogen (4% and 14% respectively). Biological studies with the use of this product in different culture media and observations on the growth and characteristics of many microbial strains have shown the possibility of its use for the diagnosis of infectious diseases, for the control of the quality of foodstuffs and water.
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The course of the reaction cited in the heading was investigated by determination of the decrease of iodometrically ascertainable oxidation capacity (pH = 7,00; initial concentration: 5 X 10(-3) M active Halogen/1, 0,5 g peptone/1). From the curves depicted in fig. 1 the following scale of reactivity can be derived: dibromoisocyanuric acid greater than bromine greater than 1,3-dibromo-5,5-dimethylhydantoin greater than N-bromo-N'-chloro-dimethylhydantoin greater than clorine greater than trichloroisocyanuric acid greater than 1,3-dichloro-5,5-dimethylhydantoin greater than iodine greater than chloramine T. The reactivity differences tentatively are explained by electronic, kinetic resp. thermodynamic effects.
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Based on 16S rRNA gene sequence comparison, an isolate that was detected in sterile-filtered vegetable broth was classified as a novel member of the Erysipelothrix line of descent of the Firmicutes. Strain MF-EP02T resembles members of the two species of Erysipelothrix with validly published names, Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum, in morphology, fatty acid composition, lack of menaquinones in aerobically and anaerobically grown cultures, DNA G+C content and peptidoglycan amino acid composition. Distinct differences in physiological characteristics, however, support the allocation of this isolate to a novel species of the genus Erysipelothrix, for which the name Erysipelothrix inopinata sp. nov. (type strain, MF-EP02T=DSM 15511T=CIP 107935T) is proposed. Members of the Erysipelothrix line of descent are included in the family Erysipelotrichaceae fam. nov.